Mutagenetix > Incidental Mutation

Incidental Mutation 'R6576:Vmn2r87'

523512

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r87

Ensembl Gene

ENSMUSG00000091511

Gene Name

vomeronasal 2, receptor 87

Synonyms

EG625131

MMRRC Submission

044700-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6576 (G1)

Quality Score

196.009

Status

Not validated

Chromosome

Chromosomal Location

130307690-130333248 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 130314654 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 311 (L311F)

Ref Sequence

ENSEMBL: ENSMUSP00000129215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164227]

AlphaFold

E9PZX4

Predicted Effect

probably benign
Transcript: ENSMUST00000164227
AA Change: L311F

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000129215
Gene: ENSMUSG00000091511
AA Change: L311F

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	422	1.8e-27	PFAM
Pfam:NCD3G	508	562	1.8e-19	PFAM
Pfam:7tm_3	595	829	8.8e-55	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.2%

Validation Efficiency

92% (34/37)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933430I17Rik	A	T	4: 62,450,842 (GRCm39)	T102S	possibly damaging	Het
Aff4	C	A	11: 53,291,268 (GRCm39)	H743N	probably damaging	Het
Apba3	A	G	10: 81,108,925 (GRCm39)	T563A	probably benign	Het
Arhgap20	T	C	9: 51,760,578 (GRCm39)	S774P	probably benign	Het
Asap2	A	G	12: 21,294,704 (GRCm39)	Y528C	probably damaging	Het
Cep162	A	G	9: 87,099,198 (GRCm39)	S767P	probably benign	Het
Ces1b	T	A	8: 93,783,547 (GRCm39)	T558S	probably benign	Het
Chd8	T	C	14: 52,453,533 (GRCm39)	Y1176C	probably damaging	Het
Cldn15	A	T	5: 137,003,470 (GRCm39)	E157D	probably damaging	Het
Col4a3	T	A	1: 82,686,295 (GRCm39)		probably null	Het
Cpsf1	CCCCTGCATGAGGCAGGTCCC	CCCC	15: 76,481,655 (GRCm39)		probably null	Het
Dnaaf3	A	G	7: 4,526,379 (GRCm39)	I566T	probably benign	Het
Drc7	A	T	8: 95,801,886 (GRCm39)	I716F	probably damaging	Het
Eddm13	G	A	7: 6,280,541 (GRCm39)		probably benign	Het
Fat3	G	A	9: 16,288,506 (GRCm39)	T339I	probably damaging	Het
Fat4	T	C	3: 39,033,839 (GRCm39)	I2497T	probably benign	Het
Fmo6	A	G	1: 162,750,264 (GRCm39)	F264S	probably damaging	Het
Gtf2i	T	C	5: 134,292,556 (GRCm39)	D356G	probably damaging	Het
Id1	T	C	2: 152,578,583 (GRCm39)	V108A	probably benign	Het
Kcnq3	T	C	15: 65,897,027 (GRCm39)	D291G	possibly damaging	Het
Klc3	G	T	7: 19,131,905 (GRCm39)	D157E	possibly damaging	Het
Lasp1	C	T	11: 97,724,402 (GRCm39)	R94C	probably damaging	Het
Lmbr1	A	T	5: 29,496,308 (GRCm39)	M93K	probably damaging	Het
Lrrc75a	G	A	11: 62,496,695 (GRCm39)	P289L	probably damaging	Het
Med11	G	T	11: 70,343,996 (GRCm39)	K105N	probably benign	Het
Mrgprx2	A	T	7: 48,132,380 (GRCm39)	I146N	probably damaging	Het
Mrpl20	A	G	4: 155,891,371 (GRCm39)	I69V	probably benign	Het
Pik3c3	A	G	18: 30,475,794 (GRCm39)		probably benign	Het
Rad54b	T	A	4: 11,601,577 (GRCm39)	N377K	probably benign	Het
Rilp	A	G	11: 75,403,218 (GRCm39)		probably null	Het
Ripk2	A	T	4: 16,131,558 (GRCm39)		probably null	Het
Rnf167	A	C	11: 70,540,588 (GRCm39)	K156T	possibly damaging	Het
Shld2	T	C	14: 33,990,199 (GRCm39)	T236A	probably damaging	Het
Snx29	T	C	16: 11,532,920 (GRCm39)		probably null	Het
Sox6	T	A	7: 115,300,937 (GRCm39)	I177F	probably damaging	Het
Tln1	A	T	4: 43,555,419 (GRCm39)		probably null	Het
Unc13a	T	A	8: 72,106,122 (GRCm39)	T661S	probably benign	Het
Vmn2r23	A	G	6: 123,710,232 (GRCm39)	T512A	probably benign	Het
Wdr64	G	T	1: 175,633,494 (GRCm39)	S915I	possibly damaging	Het
Xpo5	T	A	17: 46,551,734 (GRCm39)		probably null	Het
Zswim8	T	C	14: 20,771,942 (GRCm39)	V1548A	probably benign	Het

Other mutations in Vmn2r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r87	APN	10	130,333,247 (GRCm39)	start codon destroyed	probably null	1.00
IGL01295:Vmn2r87	APN	10	130,307,878 (GRCm39)	missense	probably damaging	1.00
IGL01411:Vmn2r87	APN	10	130,308,429 (GRCm39)	missense	probably benign	0.03
IGL01680:Vmn2r87	APN	10	130,315,586 (GRCm39)	nonsense	probably null
IGL01822:Vmn2r87	APN	10	130,307,991 (GRCm39)	missense	probably damaging	1.00
IGL01835:Vmn2r87	APN	10	130,314,978 (GRCm39)	missense	probably damaging	1.00
IGL01965:Vmn2r87	APN	10	130,314,924 (GRCm39)	missense	possibly damaging	0.49
IGL02562:Vmn2r87	APN	10	130,314,513 (GRCm39)	missense	probably damaging	1.00
IGL02665:Vmn2r87	APN	10	130,333,049 (GRCm39)	missense	probably benign	0.16
IGL03202:Vmn2r87	APN	10	130,333,091 (GRCm39)	missense	probably benign
FR4304:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4340:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4342:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4589:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
LCD18:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
R0344:Vmn2r87	UTSW	10	130,315,806 (GRCm39)	missense	probably damaging	1.00
R0374:Vmn2r87	UTSW	10	130,307,848 (GRCm39)	missense	probably damaging	1.00
R0384:Vmn2r87	UTSW	10	130,307,712 (GRCm39)	missense	probably benign
R1144:Vmn2r87	UTSW	10	130,312,098 (GRCm39)	splice site	probably benign
R1172:Vmn2r87	UTSW	10	130,313,453 (GRCm39)	missense	probably benign	0.03
R1860:Vmn2r87	UTSW	10	130,315,755 (GRCm39)	missense	probably benign	0.00
R1866:Vmn2r87	UTSW	10	130,308,441 (GRCm39)	missense	possibly damaging	0.88
R1897:Vmn2r87	UTSW	10	130,307,829 (GRCm39)	missense	probably damaging	1.00
R2360:Vmn2r87	UTSW	10	130,315,631 (GRCm39)	missense	probably damaging	0.99
R2909:Vmn2r87	UTSW	10	130,314,865 (GRCm39)	missense	probably damaging	0.99
R3874:Vmn2r87	UTSW	10	130,315,856 (GRCm39)	missense	possibly damaging	0.62
R4113:Vmn2r87	UTSW	10	130,315,691 (GRCm39)	missense	probably benign
R4190:Vmn2r87	UTSW	10	130,308,556 (GRCm39)	missense	probably damaging	1.00
R4197:Vmn2r87	UTSW	10	130,315,779 (GRCm39)	missense	possibly damaging	0.55
R4201:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4202:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4368:Vmn2r87	UTSW	10	130,315,676 (GRCm39)	missense	probably benign	0.44
R4485:Vmn2r87	UTSW	10	130,315,678 (GRCm39)	nonsense	probably null
R4537:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably benign	0.12
R4590:Vmn2r87	UTSW	10	130,315,014 (GRCm39)	missense	possibly damaging	0.69
R4752:Vmn2r87	UTSW	10	130,314,336 (GRCm39)	nonsense	probably null
R4873:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4875:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4923:Vmn2r87	UTSW	10	130,314,435 (GRCm39)	missense	probably damaging	0.99
R4970:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5049:Vmn2r87	UTSW	10	130,308,298 (GRCm39)	missense	probably damaging	0.96
R5112:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5187:Vmn2r87	UTSW	10	130,333,208 (GRCm39)	missense	probably null	0.99
R5618:Vmn2r87	UTSW	10	130,315,817 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r87	UTSW	10	130,308,226 (GRCm39)	missense	probably benign	0.02
R6220:Vmn2r87	UTSW	10	130,315,807 (GRCm39)	missense	probably benign	0.01
R6287:Vmn2r87	UTSW	10	130,314,291 (GRCm39)	critical splice donor site	probably null
R6383:Vmn2r87	UTSW	10	130,314,869 (GRCm39)	missense	probably damaging	1.00
R6742:Vmn2r87	UTSW	10	130,308,396 (GRCm39)	missense	probably damaging	1.00
R7086:Vmn2r87	UTSW	10	130,333,178 (GRCm39)	missense	probably benign	0.00
R7162:Vmn2r87	UTSW	10	130,313,416 (GRCm39)	missense	probably benign	0.08
R7419:Vmn2r87	UTSW	10	130,307,992 (GRCm39)	missense	probably damaging	1.00
R7425:Vmn2r87	UTSW	10	130,314,761 (GRCm39)	missense	probably damaging	1.00
R7443:Vmn2r87	UTSW	10	130,308,588 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r87	UTSW	10	130,314,940 (GRCm39)	missense	probably damaging	0.99
R7663:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably damaging	0.97
R7716:Vmn2r87	UTSW	10	130,308,018 (GRCm39)	missense	probably benign	0.09
R7793:Vmn2r87	UTSW	10	130,313,413 (GRCm39)	missense	probably benign	0.05
R7806:Vmn2r87	UTSW	10	130,315,679 (GRCm39)	missense	probably benign
R7841:Vmn2r87	UTSW	10	130,333,095 (GRCm39)	missense	probably benign	0.31
R8326:Vmn2r87	UTSW	10	130,308,180 (GRCm39)	missense	possibly damaging	0.67
R8411:Vmn2r87	UTSW	10	130,308,126 (GRCm39)	missense	probably damaging	0.99
R8445:Vmn2r87	UTSW	10	130,313,335 (GRCm39)	missense	probably damaging	1.00
R8540:Vmn2r87	UTSW	10	130,314,762 (GRCm39)	missense	possibly damaging	0.74
R8892:Vmn2r87	UTSW	10	130,308,105 (GRCm39)	missense	probably damaging	0.99
R9093:Vmn2r87	UTSW	10	130,308,165 (GRCm39)	missense	probably benign	0.08
R9623:Vmn2r87	UTSW	10	130,315,794 (GRCm39)	missense	probably damaging	1.00
R9667:Vmn2r87	UTSW	10	130,314,776 (GRCm39)	missense	probably damaging	1.00
R9797:Vmn2r87	UTSW	10	130,312,064 (GRCm39)	missense	probably benign	0.44
R9797:Vmn2r87	UTSW	10	130,308,138 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r87	UTSW	10	130,308,183 (GRCm39)	missense	probably damaging	0.98
Z1176:Vmn2r87	UTSW	10	130,307,713 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TTGTGCAGTGCTGTCCATTC -3'
(R):5'- TGTGATCCCAGAAACCATGAAG -3'

Sequencing Primer
(F):5'- GTGCAGTGCTGTCCATTCCAATG -3'
(R):5'- TCCCAGAAACCATGAAGATATACATG -3'

Posted On

2018-06-22