Mutagenetix > Incidental Mutation

Incidental Mutation 'R6623:Vmn2r45'

524605

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r45

Ensembl Gene

ENSMUSG00000090662

Gene Name

vomeronasal 2, receptor 45

Synonyms

MMRRC Submission

044745-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6623 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

8474468-8491958 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 8474500 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 843 (T843A)

Ref Sequence

ENSEMBL: ENSMUSP00000129466 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164845]

AlphaFold

L7N2B5

Predicted Effect

probably benign
Transcript: ENSMUST00000164845
AA Change: T843A

PolyPhen 2 Score 0.090 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000129466
Gene: ENSMUSG00000090662
AA Change: T843A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	469	4.5e-26	PFAM
Pfam:NCD3G	512	565	6.4e-21	PFAM
Pfam:7tm_3	598	833	2.1e-54	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.2%
20x: 91.1%

Validation Efficiency

100% (29/29)

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	T	C	17: 46,634,388 (GRCm39)	K460E	probably damaging	Het
Acaca	A	C	11: 84,262,325 (GRCm39)		probably null	Het
Adamts1	A	G	16: 85,592,525 (GRCm39)	S628P	probably benign	Het
Ankrd13a	A	G	5: 114,924,818 (GRCm39)	N101S	probably benign	Het
Asic5	G	A	3: 81,915,892 (GRCm39)	V281M	probably damaging	Het
Atxn7	T	C	14: 14,099,972 (GRCm38)	S553P	probably damaging	Het
Cstdc2	A	T	2: 148,692,682 (GRCm39)	I40K	probably benign	Het
Fndc3c1	G	C	X: 105,478,679 (GRCm39)	L724V	possibly damaging	Homo
Hmcn1	A	G	1: 150,634,057 (GRCm39)	F843S	probably benign	Het
Igkv10-96	T	G	6: 68,609,158 (GRCm39)	S46R	probably damaging	Het
Itfg2	G	T	6: 128,388,620 (GRCm39)	A289D	probably damaging	Het
Klc1	C	A	12: 111,772,475 (GRCm39)	N597K	probably damaging	Het
Lnx2	A	G	5: 146,961,297 (GRCm39)	V545A	probably damaging	Het
Map3k11	A	G	19: 5,745,631 (GRCm39)	I344V	probably damaging	Het
Muc6	G	A	7: 141,223,981 (GRCm39)		probably benign	Het
Myrf	C	A	19: 10,200,723 (GRCm39)	A317S	probably benign	Het
Ncoa2	A	T	1: 13,251,521 (GRCm39)	C251S	probably damaging	Het
Or52i2	T	G	7: 102,319,241 (GRCm39)	M38R	possibly damaging	Het
Pcdhb12	C	T	18: 37,570,711 (GRCm39)	T619I	possibly damaging	Het
Plxna1	A	T	6: 89,299,753 (GRCm39)	M1672K	probably damaging	Het
Prl	G	T	13: 27,245,492 (GRCm39)	V72L	probably benign	Het
Prokr2	G	T	2: 132,215,494 (GRCm39)	N161K	probably damaging	Het
Ryr2	T	C	13: 11,724,951 (GRCm39)	D2454G	probably damaging	Het
Slc2a12	T	A	10: 22,540,799 (GRCm39)	M218K	probably damaging	Het
Sorcs3	C	T	19: 48,776,944 (GRCm39)	A992V	probably benign	Het
Stxbp2	T	C	8: 3,682,561 (GRCm39)	I50T	probably damaging	Het
Supt20	A	G	3: 54,625,715 (GRCm39)	I570M	possibly damaging	Het
Sv2b	T	A	7: 74,856,132 (GRCm39)	I53F	probably damaging	Het
Vmn1r113	T	A	7: 20,521,991 (GRCm39)	M261K	probably benign	Het
Vmn1r217	T	A	13: 23,298,846 (GRCm39)	I19F	possibly damaging	Het
Vmn2r69	T	A	7: 85,056,309 (GRCm39)	I610L	possibly damaging	Het

Other mutations in Vmn2r45

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01287:Vmn2r45	APN	7	8,488,622 (GRCm39)	missense	probably benign	0.00
IGL01322:Vmn2r45	APN	7	8,484,332 (GRCm39)	missense	possibly damaging	0.49
IGL01402:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01404:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01529:Vmn2r45	APN	7	8,486,493 (GRCm39)	missense	probably benign
IGL01596:Vmn2r45	APN	7	8,486,272 (GRCm39)	missense	probably damaging	0.98
IGL01646:Vmn2r45	APN	7	8,486,337 (GRCm39)	missense	probably benign	0.18
IGL01819:Vmn2r45	APN	7	8,488,556 (GRCm39)	missense	probably benign	0.00
IGL02096:Vmn2r45	APN	7	8,484,443 (GRCm39)	missense	probably damaging	0.99
IGL02130:Vmn2r45	APN	7	8,486,556 (GRCm39)	missense	possibly damaging	0.46
IGL02409:Vmn2r45	APN	7	8,488,727 (GRCm39)	missense	probably benign	0.03
IGL02517:Vmn2r45	APN	7	8,486,185 (GRCm39)	nonsense	probably null
IGL02633:Vmn2r45	APN	7	8,488,728 (GRCm39)	missense	probably benign	0.01
IGL02940:Vmn2r45	APN	7	8,475,369 (GRCm39)	missense	probably damaging	0.99
IGL03281:Vmn2r45	APN	7	8,486,603 (GRCm39)	missense	probably damaging	1.00
IGL03358:Vmn2r45	APN	7	8,474,715 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
BB014:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R0382:Vmn2r45	UTSW	7	8,486,098 (GRCm39)	missense	probably benign	0.06
R0532:Vmn2r45	UTSW	7	8,474,820 (GRCm39)	missense	probably damaging	0.99
R0932:Vmn2r45	UTSW	7	8,478,380 (GRCm39)	missense	probably damaging	1.00
R1125:Vmn2r45	UTSW	7	8,488,542 (GRCm39)	missense	probably benign	0.00
R1580:Vmn2r45	UTSW	7	8,474,746 (GRCm39)	missense	possibly damaging	0.95
R1817:Vmn2r45	UTSW	7	8,475,372 (GRCm39)	missense	probably damaging	1.00
R1996:Vmn2r45	UTSW	7	8,475,024 (GRCm39)	missense	probably damaging	1.00
R2050:Vmn2r45	UTSW	7	8,475,021 (GRCm39)	missense	probably damaging	1.00
R2284:Vmn2r45	UTSW	7	8,488,765 (GRCm39)	missense	probably benign	0.02
R4019:Vmn2r45	UTSW	7	8,474,580 (GRCm39)	nonsense	probably null
R4227:Vmn2r45	UTSW	7	8,486,277 (GRCm39)	missense	probably damaging	0.98
R4381:Vmn2r45	UTSW	7	8,474,912 (GRCm39)	nonsense	probably null
R4618:Vmn2r45	UTSW	7	8,486,436 (GRCm39)	missense	probably benign	0.00
R4624:Vmn2r45	UTSW	7	8,484,341 (GRCm39)	missense	probably damaging	1.00
R4704:Vmn2r45	UTSW	7	8,486,535 (GRCm39)	nonsense	probably null
R4735:Vmn2r45	UTSW	7	8,486,472 (GRCm39)	missense	probably damaging	1.00
R4868:Vmn2r45	UTSW	7	8,484,480 (GRCm39)	missense	probably benign	0.06
R4983:Vmn2r45	UTSW	7	8,486,116 (GRCm39)	missense	probably damaging	0.99
R5246:Vmn2r45	UTSW	7	8,486,251 (GRCm39)	missense	probably benign	0.00
R5430:Vmn2r45	UTSW	7	8,486,333 (GRCm39)	nonsense	probably null
R5504:Vmn2r45	UTSW	7	8,486,176 (GRCm39)	missense	probably benign	0.03
R5511:Vmn2r45	UTSW	7	8,474,832 (GRCm39)	missense	probably benign	0.19
R5745:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R5814:Vmn2r45	UTSW	7	8,474,475 (GRCm39)	missense	probably benign	0.00
R6223:Vmn2r45	UTSW	7	8,486,301 (GRCm39)	missense	probably benign
R6267:Vmn2r45	UTSW	7	8,475,207 (GRCm39)	missense	probably benign	0.26
R6999:Vmn2r45	UTSW	7	8,486,219 (GRCm39)	missense	probably benign
R7242:Vmn2r45	UTSW	7	8,488,612 (GRCm39)	nonsense	probably null
R7491:Vmn2r45	UTSW	7	8,484,342 (GRCm39)	missense	probably benign	0.27
R7620:Vmn2r45	UTSW	7	8,486,222 (GRCm39)	nonsense	probably null
R7719:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7720:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7853:Vmn2r45	UTSW	7	8,485,987 (GRCm39)	missense	possibly damaging	0.90
R7873:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R7882:Vmn2r45	UTSW	7	8,486,409 (GRCm39)	missense	possibly damaging	0.82
R7927:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R8684:Vmn2r45	UTSW	7	8,486,511 (GRCm39)	missense	probably damaging	0.99
R8780:Vmn2r45	UTSW	7	8,484,371 (GRCm39)	missense	possibly damaging	0.94
R8811:Vmn2r45	UTSW	7	8,474,881 (GRCm39)	missense	probably damaging	1.00
R8893:Vmn2r45	UTSW	7	8,488,619 (GRCm39)	missense	probably damaging	1.00
R9043:Vmn2r45	UTSW	7	8,474,904 (GRCm39)	missense	possibly damaging	0.92
R9208:Vmn2r45	UTSW	7	8,486,298 (GRCm39)	missense	probably damaging	1.00
R9327:Vmn2r45	UTSW	7	8,474,532 (GRCm39)	missense	probably damaging	1.00
R9586:Vmn2r45	UTSW	7	8,486,050 (GRCm39)	missense	probably benign	0.01
R9599:Vmn2r45	UTSW	7	8,478,457 (GRCm39)	critical splice acceptor site	probably null
U24488:Vmn2r45	UTSW	7	8,475,361 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r45	UTSW	7	8,474,484 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- TTTATGGACACTGTAGGGAAGTTC -3'
(R):5'- TGACGCATTCAATGAAGCCAAG -3'

Sequencing Primer
(F):5'- CACTGTAGGGAAGTTCAGTAGATATG -3'
(R):5'- GAAGCCAAGTTCTTGACCTTCAG -3'

Posted On

2018-06-22