Mutagenetix > Incidental Mutation

Incidental Mutation 'R6595:Vmn2r18'

524857

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r18

Ensembl Gene

ENSMUSG00000091794

Gene Name

vomeronasal 2, receptor 18

Synonyms

EG632671

MMRRC Submission

044719-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R6595 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

151485126-151529232 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 151485889 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 535 (T535I)

Ref Sequence

ENSEMBL: ENSMUSP00000127506 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165928]

AlphaFold

A0A3B2WB67

Predicted Effect

probably damaging
Transcript: ENSMUST00000165928
AA Change: T535I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127506
Gene: ENSMUSG00000091794
AA Change: T535I

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	403	5.7e-39	PFAM
Pfam:NCD3G	446	499	5.5e-20	PFAM
Pfam:7tm_3	531	767	5e-53	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.5%

Validation Efficiency

97% (37/38)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	A	T	7: 119,993,710 (GRCm39)	Y1310F	probably benign	Het
Ankrd54	A	G	15: 78,942,185 (GRCm39)	F148L	probably damaging	Het
Bag4	C	T	8: 26,259,528 (GRCm39)	D224N	probably damaging	Het
Bhlhe40	TG	TGG	6: 108,641,818 (GRCm39)	254	probably null	Het
Camk2b	A	T	11: 5,942,856 (GRCm39)	H126Q	probably damaging	Het
Camsap3	G	T	8: 3,654,186 (GRCm39)	V608L	probably damaging	Het
Camsap3	A	T	8: 3,658,742 (GRCm39)	M796L	probably damaging	Het
Cdh19	T	C	1: 110,853,517 (GRCm39)	D308G	probably benign	Het
Cfap299	A	T	5: 98,949,717 (GRCm39)	D217V	possibly damaging	Het
Cpsf1	T	C	15: 76,486,710 (GRCm39)	I275M	probably damaging	Het
Cuta	A	G	17: 27,157,856 (GRCm39)		probably null	Het
Dclk2	A	G	3: 86,699,374 (GRCm39)		probably benign	Het
Dst	T	G	1: 34,289,761 (GRCm39)	L784R	probably damaging	Het
Fbn1	T	C	2: 125,184,750 (GRCm39)	M1681V	possibly damaging	Het
Fbxo9	A	T	9: 77,994,494 (GRCm39)	D274E	probably damaging	Het
Frem2	T	A	3: 53,457,205 (GRCm39)	D2049V	probably damaging	Het
Fscn3	T	C	6: 28,430,174 (GRCm39)	Y115H	probably damaging	Het
Glp2r	G	T	11: 67,655,603 (GRCm39)	D46E	probably benign	Het
Gpat2	G	C	2: 127,273,838 (GRCm39)	G294R	possibly damaging	Het
Irx5	A	G	8: 93,086,247 (GRCm39)	Y110C	probably damaging	Het
Kdm5d	T	C	Y: 939,829 (GRCm39)	S994P	probably benign	Homo
Klhl2	A	T	8: 65,196,077 (GRCm39)	C555*	probably null	Het
Krtap4-7	A	T	11: 99,534,560 (GRCm39)	I101N	unknown	Het
Or13a27	A	G	7: 139,925,560 (GRCm39)	L114P	probably damaging	Het
Or4f57	A	T	2: 111,790,515 (GRCm39)	V301E	possibly damaging	Het
Pcdhb21	T	C	18: 37,648,961 (GRCm39)	S697P	probably damaging	Het
Pramel27	T	C	4: 143,579,326 (GRCm39)	C304R	probably damaging	Het
Rasgrf2	A	T	13: 92,167,361 (GRCm39)	H237Q	probably damaging	Het
Rnf216	A	T	5: 143,076,412 (GRCm39)	D157E	probably benign	Het
Rxrg	T	A	1: 167,454,905 (GRCm39)	F163I	probably damaging	Het
Soat2	T	A	15: 102,069,028 (GRCm39)	I351N	probably damaging	Het
Srp72	C	T	5: 77,132,047 (GRCm39)	T242I	probably benign	Het
Svopl	T	C	6: 38,018,002 (GRCm39)		probably null	Het
Tbc1d2b	G	A	9: 90,108,145 (GRCm39)	P469S	probably benign	Het
Tbkbp1	G	A	11: 97,029,578 (GRCm39)		probably benign	Het
Tecta	A	G	9: 42,295,523 (GRCm39)	V324A	probably damaging	Het
Twnk	T	C	19: 44,998,931 (GRCm39)	V557A	probably damaging	Het
Zc3h14	T	A	12: 98,723,285 (GRCm39)	S85T	probably damaging	Het

Other mutations in Vmn2r18

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01098:Vmn2r18	APN	5	151,496,296 (GRCm39)	missense	probably damaging	0.99
IGL01564:Vmn2r18	APN	5	151,508,633 (GRCm39)	missense	possibly damaging	0.69
IGL01602:Vmn2r18	APN	5	151,510,106 (GRCm39)	missense	possibly damaging	0.64
IGL01605:Vmn2r18	APN	5	151,510,106 (GRCm39)	missense	possibly damaging	0.64
IGL01619:Vmn2r18	APN	5	151,510,229 (GRCm39)	missense	probably benign	0.25
IGL02177:Vmn2r18	APN	5	151,510,274 (GRCm39)	missense	possibly damaging	0.76
IGL02682:Vmn2r18	APN	5	151,508,102 (GRCm39)	missense	probably damaging	0.99
IGL02751:Vmn2r18	APN	5	151,508,072 (GRCm39)	missense	probably benign	0.09
IGL02977:Vmn2r18	APN	5	151,510,149 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r18	UTSW	5	151,508,269 (GRCm39)	missense	probably benign	0.00
R0282:Vmn2r18	UTSW	5	151,508,668 (GRCm39)	missense	probably benign	0.02
R0529:Vmn2r18	UTSW	5	151,485,988 (GRCm39)	missense	probably damaging	0.99
R0812:Vmn2r18	UTSW	5	151,496,395 (GRCm39)	splice site	probably benign
R1467:Vmn2r18	UTSW	5	151,510,301 (GRCm39)	missense	possibly damaging	0.48
R1467:Vmn2r18	UTSW	5	151,510,301 (GRCm39)	missense	possibly damaging	0.48
R1506:Vmn2r18	UTSW	5	151,499,099 (GRCm39)	splice site	probably null
R1562:Vmn2r18	UTSW	5	151,510,301 (GRCm39)	missense	probably benign	0.12
R1637:Vmn2r18	UTSW	5	151,508,222 (GRCm39)	missense	probably damaging	1.00
R1651:Vmn2r18	UTSW	5	151,485,464 (GRCm39)	missense	probably damaging	1.00
R1883:Vmn2r18	UTSW	5	151,499,190 (GRCm39)	missense	probably benign	0.37
R1884:Vmn2r18	UTSW	5	151,499,190 (GRCm39)	missense	probably benign	0.37
R1914:Vmn2r18	UTSW	5	151,499,286 (GRCm39)	missense	probably benign	0.02
R2051:Vmn2r18	UTSW	5	151,486,016 (GRCm39)	missense	possibly damaging	0.90
R2056:Vmn2r18	UTSW	5	151,508,160 (GRCm39)	missense	probably damaging	0.98
R2252:Vmn2r18	UTSW	5	151,508,441 (GRCm39)	missense	possibly damaging	0.59
R2265:Vmn2r18	UTSW	5	151,510,127 (GRCm39)	missense	probably damaging	1.00
R2266:Vmn2r18	UTSW	5	151,510,127 (GRCm39)	missense	probably damaging	1.00
R2267:Vmn2r18	UTSW	5	151,510,127 (GRCm39)	missense	probably damaging	1.00
R2843:Vmn2r18	UTSW	5	151,485,749 (GRCm39)	missense	probably damaging	1.00
R3023:Vmn2r18	UTSW	5	151,485,148 (GRCm39)	missense	probably benign	0.13
R3694:Vmn2r18	UTSW	5	151,508,033 (GRCm39)	missense	probably benign	0.00
R3763:Vmn2r18	UTSW	5	151,508,644 (GRCm39)	missense	probably damaging	1.00
R3816:Vmn2r18	UTSW	5	151,485,148 (GRCm39)	missense	probably benign	0.13
R4007:Vmn2r18	UTSW	5	151,508,711 (GRCm39)	missense	probably damaging	0.99
R4152:Vmn2r18	UTSW	5	151,485,730 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r18	UTSW	5	151,508,268 (GRCm39)	nonsense	probably null
R4362:Vmn2r18	UTSW	5	151,496,368 (GRCm39)	missense	probably damaging	1.00
R4618:Vmn2r18	UTSW	5	151,508,424 (GRCm39)	missense	possibly damaging	0.64
R4716:Vmn2r18	UTSW	5	151,485,602 (GRCm39)	missense	possibly damaging	0.87
R4817:Vmn2r18	UTSW	5	151,508,897 (GRCm39)	splice site	probably null
R5111:Vmn2r18	UTSW	5	151,485,913 (GRCm39)	missense	possibly damaging	0.85
R5692:Vmn2r18	UTSW	5	151,485,724 (GRCm39)	missense	possibly damaging	0.65
R6115:Vmn2r18	UTSW	5	151,508,462 (GRCm39)	missense	possibly damaging	0.81
R6244:Vmn2r18	UTSW	5	151,508,116 (GRCm39)	missense	probably damaging	1.00
R6997:Vmn2r18	UTSW	5	151,485,338 (GRCm39)	missense	possibly damaging	0.95
R7227:Vmn2r18	UTSW	5	151,496,264 (GRCm39)	missense	probably damaging	1.00
R7349:Vmn2r18	UTSW	5	151,485,682 (GRCm39)	nonsense	probably null
R7506:Vmn2r18	UTSW	5	151,508,485 (GRCm39)	missense	possibly damaging	0.51
R7590:Vmn2r18	UTSW	5	151,485,194 (GRCm39)	missense	probably damaging	1.00
R7721:Vmn2r18	UTSW	5	151,510,158 (GRCm39)	missense	possibly damaging	0.53
R7877:Vmn2r18	UTSW	5	151,508,437 (GRCm39)	missense	probably damaging	1.00
R7882:Vmn2r18	UTSW	5	151,485,329 (GRCm39)	missense	probably damaging	1.00
R7984:Vmn2r18	UTSW	5	151,485,526 (GRCm39)	missense	probably damaging	1.00
R8295:Vmn2r18	UTSW	5	151,508,621 (GRCm39)	missense	probably damaging	0.97
R8353:Vmn2r18	UTSW	5	151,485,373 (GRCm39)	missense	probably damaging	1.00
R8453:Vmn2r18	UTSW	5	151,485,373 (GRCm39)	missense	probably damaging	1.00
R8670:Vmn2r18	UTSW	5	151,485,854 (GRCm39)	missense	probably damaging	1.00
R8725:Vmn2r18	UTSW	5	151,508,462 (GRCm39)	missense	probably damaging	0.99
R8727:Vmn2r18	UTSW	5	151,508,462 (GRCm39)	missense	probably damaging	0.99
R8895:Vmn2r18	UTSW	5	151,485,140 (GRCm39)	missense	possibly damaging	0.46
R9286:Vmn2r18	UTSW	5	151,499,175 (GRCm39)	missense	probably benign	0.00
R9339:Vmn2r18	UTSW	5	151,485,132 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r18	UTSW	5	151,508,498 (GRCm39)	missense	probably damaging	0.99
Z1187:Vmn2r18	UTSW	5	151,508,497 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CCAATGTGAACACAAGTGCAAATG -3'
(R):5'- TGTGCCAGGCTAAACACAAC -3'

Sequencing Primer
(F):5'- CAAATGTTATTTGTTGCAGTATGCAG -3'
(R):5'- TGTGCCAGGCTAAACACAACATAAG -3'

Posted On

2018-06-22