Incidental Mutation 'R6603:Miip'
| ID |
525434 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Miip
|
| Ensembl Gene |
ENSMUSG00000029022 |
| Gene Name |
migration and invasion inhibitory protein |
| Synonyms |
D4Wsu114e |
| MMRRC Submission |
044726-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.078)
|
| Stock # |
R6603 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
4 |
| Chromosomal Location |
147945235-147953176 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to G
at 147950380 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Lysine to Threonine
at position 72
(K72T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000134085
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000030886]
[ENSMUST00000094481]
[ENSMUST00000119975]
[ENSMUST00000172710]
|
| AlphaFold |
A2A7Y5 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000030886
AA Change: K72T
PolyPhen 2
Score 0.923 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: K72T
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
13 |
24 |
N/A |
INTRINSIC |
|
low complexity region
|
60 |
69 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000094481
|
| SMART Domains |
Protein: ENSMUSP00000092054
Gene: ENSMUSG00000070583
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
22 |
32 |
N/A |
INTRINSIC |
|
coiled coil region
|
86 |
116 |
N/A |
INTRINSIC |
|
low complexity region
|
438 |
451 |
N/A |
INTRINSIC |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000119975
AA Change: K72T
PolyPhen 2
Score 0.923 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: K72T
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
13 |
24 |
N/A |
INTRINSIC |
|
Pfam:MIIP
|
41 |
382 |
1.4e-142 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000141166
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000141534
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000156374
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000172710
AA Change: K72T
PolyPhen 2
Score 0.923 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: K72T
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
13 |
24 |
N/A |
INTRINSIC |
|
low complexity region
|
60 |
69 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000174081
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000173034
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.5%
- 10x: 97.7%
- 20x: 92.9%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 56 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam18 |
A |
T |
8: 25,155,518 (GRCm39) |
F167Y |
possibly damaging |
Het |
| Ap3d1 |
A |
G |
10: 80,549,881 (GRCm39) |
S755P |
probably benign |
Het |
| Aph1a |
A |
T |
3: 95,802,808 (GRCm39) |
N136I |
probably damaging |
Het |
| AU040320 |
T |
A |
4: 126,686,046 (GRCm39) |
N207K |
probably benign |
Het |
| Caprin1 |
A |
T |
2: 103,605,856 (GRCm39) |
D377E |
probably benign |
Het |
| Col18a1 |
A |
G |
10: 76,899,811 (GRCm39) |
|
probably null |
Het |
| Ddi2 |
T |
C |
4: 141,411,181 (GRCm39) |
N577S |
probably damaging |
Het |
| Dip2c |
T |
A |
13: 9,704,624 (GRCm39) |
|
probably null |
Het |
| Dmbt1 |
A |
T |
7: 130,648,240 (GRCm39) |
|
probably null |
Het |
| Fam13a |
T |
C |
6: 58,964,174 (GRCm39) |
K86R |
probably benign |
Het |
| Fam168b |
C |
A |
1: 34,875,822 (GRCm39) |
G21V |
probably damaging |
Het |
| Fbxl8 |
A |
T |
8: 105,994,842 (GRCm39) |
D118V |
probably damaging |
Het |
| Garin5b |
G |
A |
7: 4,761,431 (GRCm39) |
P427L |
possibly damaging |
Het |
| Git2 |
C |
A |
5: 114,869,052 (GRCm39) |
|
probably null |
Het |
| Gm17190 |
T |
G |
13: 96,218,770 (GRCm39) |
D35E |
possibly damaging |
Het |
| Gnb4 |
C |
T |
3: 32,639,295 (GRCm39) |
D333N |
probably damaging |
Het |
| Has2 |
T |
A |
15: 56,531,968 (GRCm39) |
D249V |
probably damaging |
Het |
| Ighv1-23 |
A |
G |
12: 114,728,141 (GRCm39) |
S94P |
probably damaging |
Het |
| Itpr2 |
A |
G |
6: 146,248,669 (GRCm39) |
I1029T |
probably damaging |
Het |
| Kctd16 |
T |
C |
18: 40,391,544 (GRCm39) |
I44T |
probably benign |
Het |
| Kpna1 |
A |
G |
16: 35,849,890 (GRCm39) |
|
probably null |
Het |
| Lonrf1 |
T |
A |
8: 36,690,095 (GRCm39) |
R654S |
probably damaging |
Het |
| Lrrc49 |
A |
C |
9: 60,501,052 (GRCm39) |
|
probably null |
Het |
| Mink1 |
C |
T |
11: 70,500,419 (GRCm39) |
P782S |
probably damaging |
Het |
| Mpped2 |
A |
G |
2: 106,697,322 (GRCm39) |
T266A |
probably benign |
Het |
| Naip1 |
G |
A |
13: 100,559,578 (GRCm39) |
S1142F |
probably benign |
Het |
| Naip1 |
C |
T |
13: 100,559,666 (GRCm39) |
G1113S |
probably benign |
Het |
| Nbr1 |
A |
G |
11: 101,446,931 (GRCm39) |
|
probably benign |
Het |
| Necab3 |
A |
T |
2: 154,396,842 (GRCm39) |
N46K |
probably damaging |
Het |
| Or51a8 |
A |
T |
7: 102,549,621 (GRCm39) |
I16F |
probably benign |
Het |
| Phtf1 |
C |
T |
3: 103,901,189 (GRCm39) |
R360C |
probably damaging |
Het |
| Plcl2 |
A |
G |
17: 50,914,145 (GRCm39) |
I385V |
probably benign |
Het |
| Prmt8 |
A |
T |
6: 127,706,376 (GRCm39) |
F138L |
probably benign |
Het |
| Prpf40a |
T |
G |
2: 53,042,975 (GRCm39) |
R436S |
probably damaging |
Het |
| Rab27b |
T |
A |
18: 70,118,375 (GRCm39) |
I181F |
probably damaging |
Het |
| Rasgrf1 |
A |
G |
9: 89,792,310 (GRCm39) |
E87G |
probably damaging |
Het |
| Scrib |
T |
A |
15: 75,934,572 (GRCm39) |
T674S |
probably benign |
Het |
| Slc9a4 |
A |
G |
1: 40,662,664 (GRCm39) |
S644G |
probably benign |
Het |
| Slc9a9 |
G |
A |
9: 94,821,599 (GRCm39) |
A329T |
probably damaging |
Het |
| Smc4 |
T |
C |
3: 68,929,794 (GRCm39) |
|
probably null |
Het |
| Sox7 |
T |
A |
14: 64,185,637 (GRCm39) |
H224Q |
probably benign |
Het |
| Spata31 |
T |
C |
13: 65,070,479 (GRCm39) |
S876P |
probably damaging |
Het |
| Syndig1 |
G |
A |
2: 149,845,208 (GRCm39) |
V244M |
probably damaging |
Het |
| Tas2r113 |
A |
T |
6: 132,870,421 (GRCm39) |
I150L |
probably benign |
Het |
| Tasor |
A |
G |
14: 27,168,343 (GRCm39) |
Y295C |
probably damaging |
Het |
| Tmem59l |
G |
A |
8: 70,939,006 (GRCm39) |
P56L |
probably benign |
Het |
| Tnfrsf8 |
A |
T |
4: 145,019,168 (GRCm39) |
D222E |
possibly damaging |
Het |
| Trim52 |
T |
C |
14: 106,344,483 (GRCm39) |
L47P |
probably damaging |
Het |
| Ttc34 |
T |
A |
4: 154,923,762 (GRCm39) |
I157K |
probably benign |
Het |
| Txndc16 |
A |
G |
14: 45,389,224 (GRCm39) |
F492S |
probably damaging |
Het |
| Ubr4 |
A |
G |
4: 139,182,897 (GRCm39) |
I428V |
probably benign |
Het |
| Vmn2r41 |
T |
A |
7: 8,141,359 (GRCm39) |
I702F |
probably damaging |
Het |
| Wdr12 |
T |
A |
1: 60,121,783 (GRCm39) |
H256L |
probably damaging |
Het |
| Xirp2 |
A |
G |
2: 67,346,888 (GRCm39) |
H3043R |
probably benign |
Het |
| Xrcc1 |
C |
T |
7: 24,270,459 (GRCm39) |
Q500* |
probably null |
Het |
| Zfp583 |
T |
C |
7: 6,328,475 (GRCm39) |
N38S |
probably damaging |
Het |
|
| Other mutations in Miip |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00531:Miip
|
APN |
4 |
147,950,322 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02134:Miip
|
APN |
4 |
147,949,735 (GRCm39) |
splice site |
probably benign |
|
|
IGL02829:Miip
|
APN |
4 |
147,947,518 (GRCm39) |
missense |
probably benign |
0.01 |
|
IGL03350:Miip
|
APN |
4 |
147,946,979 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0200:Miip
|
UTSW |
4 |
147,946,720 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1647:Miip
|
UTSW |
4 |
147,949,691 (GRCm39) |
missense |
probably benign |
0.02 |
|
R1783:Miip
|
UTSW |
4 |
147,950,231 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1848:Miip
|
UTSW |
4 |
147,947,549 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1944:Miip
|
UTSW |
4 |
147,950,422 (GRCm39) |
missense |
probably benign |
0.15 |
|
R3615:Miip
|
UTSW |
4 |
147,950,371 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3616:Miip
|
UTSW |
4 |
147,950,371 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3882:Miip
|
UTSW |
4 |
147,945,509 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R4579:Miip
|
UTSW |
4 |
147,945,518 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5183:Miip
|
UTSW |
4 |
147,947,526 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6054:Miip
|
UTSW |
4 |
147,950,135 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6056:Miip
|
UTSW |
4 |
147,946,792 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6304:Miip
|
UTSW |
4 |
147,947,540 (GRCm39) |
missense |
probably benign |
0.12 |
|
R6568:Miip
|
UTSW |
4 |
147,950,372 (GRCm39) |
missense |
probably benign |
|
|
R7639:Miip
|
UTSW |
4 |
147,947,021 (GRCm39) |
missense |
probably benign |
0.22 |
|
R7701:Miip
|
UTSW |
4 |
147,947,371 (GRCm39) |
missense |
probably null |
0.86 |
|
R7795:Miip
|
UTSW |
4 |
147,947,375 (GRCm39) |
missense |
probably benign |
0.17 |
|
R7796:Miip
|
UTSW |
4 |
147,947,375 (GRCm39) |
missense |
probably benign |
0.17 |
|
R7797:Miip
|
UTSW |
4 |
147,947,375 (GRCm39) |
missense |
probably benign |
0.17 |
|
R7872:Miip
|
UTSW |
4 |
147,947,375 (GRCm39) |
missense |
probably benign |
0.17 |
|
R7920:Miip
|
UTSW |
4 |
147,947,375 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8468:Miip
|
UTSW |
4 |
147,945,928 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8492:Miip
|
UTSW |
4 |
147,945,881 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8677:Miip
|
UTSW |
4 |
147,947,503 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8852:Miip
|
UTSW |
4 |
147,950,839 (GRCm39) |
start gained |
probably benign |
|
|
R8860:Miip
|
UTSW |
4 |
147,950,839 (GRCm39) |
start gained |
probably benign |
|
|
R9755:Miip
|
UTSW |
4 |
147,950,319 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TGTCTTGTCAGGTCCCAGAC -3'
(R):5'- CAGCAGGGTAAATTACAAGGTCC -3'
Sequencing Primer
(F):5'- TGGTCCTCCCAAAGACACTGG -3'
(R):5'- GGTAAATTACAAGGTCCACAGC -3'
|
| Posted On |
2018-06-22 |
Incidental Mutation