Incidental Mutation 'R6654:Gsta4'
ID 526561
Institutional Source Beutler Lab
Gene Symbol Gsta4
Ensembl Gene ENSMUSG00000032348
Gene Name glutathione S-transferase, alpha 4
Synonyms GST 5.7, mGsta4
MMRRC Submission 044775-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6654 (G1)
Quality Score 225.009
Status Validated
Chromosome 9
Chromosomal Location 78099248-78116631 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 78116381 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 197 (F197L)
Ref Sequence ENSEMBL: ENSMUSP00000034903 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034903] [ENSMUST00000213215]
AlphaFold P24472
PDB Structure CRYSTAL STRUCTURE OF MGSTA4-4 IN COMPLEX WITH GSH CONJUGATE OF 4-HYDROXYNONENAL IN ONE SUBUNIT AND GSH IN THE OTHER: EVIDENCE OF SIGNALING ACROSS DIMER INTERFACE IN MGSTA4-4 [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF MURINE ALPHA-CLASS GSTA4-4 [X-RAY DIFFRACTION]
Predicted Effect probably damaging
Transcript: ENSMUST00000034903
AA Change: F197L

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000034903
Gene: ENSMUSG00000032348
AA Change: F197L

DomainStartEndE-ValueType
Pfam:GST_N 5 77 1.2e-19 PFAM
Pfam:GST_C 99 192 5.2e-20 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000213215
Predicted Effect noncoding transcript
Transcript: ENSMUST00000216513
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.0%
  • 20x: 94.0%
Validation Efficiency 97% (35/36)
MGI Phenotype PHENOTYPE: Mice homozygous for disruptions in this gene display increased sensitivity to oxidative stress, increased alanine and aspartate transaminase levels, increased susceptibility to bacterial infection, reduced litter size, and impaired glucose tolerance. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 35 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Akr1b1 C T 6: 34,286,939 (GRCm39) V206M possibly damaging Het
Armc6 T C 8: 70,684,025 (GRCm39) E9G probably damaging Het
Bhlhe40 TG TGG 6: 108,641,818 (GRCm39) 254 probably null Het
Ddc A T 11: 11,830,452 (GRCm39) I64N probably damaging Het
Exd1 T A 2: 119,355,198 (GRCm39) probably null Het
Gm4847 C A 1: 166,457,956 (GRCm39) G466C probably damaging Het
Gm5431 T C 11: 48,785,427 (GRCm39) D316G possibly damaging Het
Irs2 A G 8: 11,056,486 (GRCm39) Y649H probably damaging Het
Kif16b T C 2: 142,543,197 (GRCm39) probably benign Het
Krtap12-1 T C 10: 77,556,537 (GRCm39) probably benign Het
Ktn1 G A 14: 47,927,457 (GRCm39) S537N probably damaging Het
Med12l G T 3: 59,169,713 (GRCm39) G1626W probably damaging Het
Mfng T A 15: 78,643,539 (GRCm39) T223S probably damaging Het
Msh3 T C 13: 92,481,550 (GRCm39) T321A probably benign Het
Myo7b T C 18: 32,123,322 (GRCm39) I672V possibly damaging Het
Nbas C T 12: 13,533,875 (GRCm39) Q1837* probably null Het
Nlrc4 G A 17: 74,752,523 (GRCm39) A620V possibly damaging Het
Nubpl T A 12: 52,357,516 (GRCm39) V310E probably damaging Het
Or4p21 T G 2: 88,277,016 (GRCm39) T89P possibly damaging Het
Or5aq6 A G 2: 86,923,394 (GRCm39) S116P probably benign Het
P2ry12 A G 3: 59,125,441 (GRCm39) L78P probably damaging Het
Pira1 A T 7: 3,738,928 (GRCm39) S560T probably benign Het
Pkd1l3 T G 8: 110,350,915 (GRCm39) S587A probably benign Het
Potefam1 T A 2: 111,002,229 (GRCm39) M154L unknown Het
Prkacb A G 3: 146,456,298 (GRCm39) V145A possibly damaging Het
Rpgrip1l T C 8: 91,946,833 (GRCm39) E1256G probably benign Het
Rsph4a A G 10: 33,788,988 (GRCm39) Q611R probably benign Het
Sorl1 T C 9: 41,891,941 (GRCm39) D1903G possibly damaging Het
Tmem39b A T 4: 129,580,619 (GRCm39) V291D probably damaging Het
Unc79 A C 12: 103,045,307 (GRCm39) K685Q probably damaging Het
Unc79 A T 12: 103,045,308 (GRCm39) K685I probably damaging Het
Vmn2r111 T C 17: 22,778,032 (GRCm39) N549S possibly damaging Het
Vmn2r112 G T 17: 22,822,450 (GRCm39) S376I possibly damaging Het
Zfp850 A T 7: 27,684,640 (GRCm39) C35* probably null Het
Zfp974 A G 7: 27,625,828 (GRCm39) V14A probably damaging Het
Other mutations in Gsta4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01137:Gsta4 APN 9 78,113,204 (GRCm39) missense possibly damaging 0.63
IGL02442:Gsta4 APN 9 78,116,447 (GRCm39) missense probably benign
IGL03088:Gsta4 APN 9 78,113,345 (GRCm39) unclassified probably benign
R1668:Gsta4 UTSW 9 78,111,570 (GRCm39) missense probably benign 0.00
R1766:Gsta4 UTSW 9 78,111,611 (GRCm39) nonsense probably null
R4578:Gsta4 UTSW 9 78,113,302 (GRCm39) missense probably benign 0.12
R8190:Gsta4 UTSW 9 78,105,654 (GRCm39) missense possibly damaging 0.80
R8825:Gsta4 UTSW 9 78,116,121 (GRCm39) unclassified probably benign
R9181:Gsta4 UTSW 9 78,105,597 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CTAGGCGTTGTCTGTTAAGAAC -3'
(R):5'- CTATATACCTTGCTTCTGGAATGC -3'

Sequencing Primer
(F):5'- GCCATCTGTCTGCATGTAAATG -3'
(R):5'- TCTGGAATGCTCTGCAGC -3'
Posted On 2018-07-23