Mutagenetix > Incidental Mutation

Incidental Mutation 'R6664:Map3k21'

526908

Institutional Source

Beutler Lab

Gene Symbol

Map3k21

Ensembl Gene

ENSMUSG00000031853

Gene Name

mitogen-activated protein kinase kinase kinase 21

Synonyms

BC021891

MMRRC Submission

044784-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.151) question?

Stock #

R6664 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

126637189-126674179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 126668610 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 732 (E732G)

Ref Sequence

ENSEMBL: ENSMUSP00000034316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034316]

AlphaFold

Q8VDG6

Predicted Effect

probably benign
Transcript: ENSMUST00000034316
AA Change: E732G

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000034316
Gene: ENSMUSG00000031853
AA Change: E732G

Domain	Start	End	E-Value	Type
SH3	27	87	1.1e-18	SMART
TyrKc	110	382	6.04e-82	SMART
coiled coil region	402	474	N/A	INTRINSIC
low complexity region	478	492	N/A	INTRINSIC
low complexity region	661	677	N/A	INTRINSIC
low complexity region	740	758	N/A	INTRINSIC
low complexity region	766	788	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm5	T	C	4: 144,503,969 (GRCm39)	*394W	probably null	Het
AU040320	A	T	4: 126,729,443 (GRCm39)	Y535F	probably damaging	Het
Auh	G	C	13: 53,052,703 (GRCm39)	S138W	probably damaging	Het
Bmp2k	A	T	5: 97,235,989 (GRCm39)	K1137N	probably benign	Het
Btn1a1	A	G	13: 23,643,490 (GRCm39)	Y320H	probably benign	Het
Ccdc18	C	T	5: 108,315,966 (GRCm39)	Q479*	probably null	Het
Cyp4f18	A	T	8: 72,743,759 (GRCm39)	S399T	probably benign	Het
Dcaf13	T	C	15: 38,982,283 (GRCm39)	L83P	probably damaging	Het
Fbxo45	T	C	16: 32,057,234 (GRCm39)	N220S	probably damaging	Het
Fbxw16	T	C	9: 109,267,326 (GRCm39)	M302V	probably benign	Het
Fgb	A	T	3: 82,954,066 (GRCm39)	S57R	probably damaging	Het
Fryl	A	G	5: 73,289,824 (GRCm39)	V134A	probably damaging	Het
Gatad2a	A	G	8: 70,370,139 (GRCm39)	L189P	probably damaging	Het
Ifitm7	T	C	16: 13,801,613 (GRCm39)	T49A	probably benign	Het
Itih1	T	C	14: 30,655,393 (GRCm39)	T601A	probably damaging	Het
Jmjd4	T	C	11: 59,341,245 (GRCm39)	F59L	probably benign	Het
Magi2	C	T	5: 20,907,395 (GRCm39)	S1323L	probably benign	Het
Nr4a3	G	A	4: 48,056,006 (GRCm39)	R344Q	probably damaging	Het
Or1j12	A	G	2: 36,343,110 (GRCm39)	N171S	probably benign	Het
Or52e5	T	A	7: 104,719,395 (GRCm39)	S240R	possibly damaging	Het
Pik3cb	T	C	9: 98,976,591 (GRCm39)	T169A	possibly damaging	Het
Prl3a1	C	A	13: 27,454,194 (GRCm39)	Y57*	probably null	Het
Ripor2	A	T	13: 24,859,803 (GRCm39)	I164F	probably damaging	Het
Sik2	T	C	9: 50,846,757 (GRCm39)	E146G	probably damaging	Het
Szt2	G	A	4: 118,248,942 (GRCm39)	R581C	probably damaging	Het
Tango6	T	A	8: 107,468,746 (GRCm39)	D747E	probably damaging	Het
Vmn2r111	T	C	17: 22,778,032 (GRCm39)	N549S	possibly damaging	Het
Zfp945	A	G	17: 23,071,339 (GRCm39)	S187P	probably damaging	Het

Other mutations in Map3k21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00494:Map3k21	APN	8	126,671,412 (GRCm39)	missense	possibly damaging	0.52
IGL01919:Map3k21	APN	8	126,668,871 (GRCm39)	missense	probably damaging	0.97
IGL02065:Map3k21	APN	8	126,668,397 (GRCm39)	missense	probably benign	0.01
IGL02123:Map3k21	APN	8	126,652,849 (GRCm39)	missense	probably damaging	1.00
IGL02127:Map3k21	APN	8	126,668,886 (GRCm39)	missense	probably benign
IGL02863:Map3k21	APN	8	126,654,280 (GRCm39)	missense	probably benign	0.02
IGL03194:Map3k21	APN	8	126,650,801 (GRCm39)	missense	possibly damaging	0.90
PIT4142001:Map3k21	UTSW	8	126,664,047 (GRCm39)	missense	probably damaging	0.98
R0238:Map3k21	UTSW	8	126,671,709 (GRCm39)	missense	possibly damaging	0.67
R0238:Map3k21	UTSW	8	126,671,709 (GRCm39)	missense	possibly damaging	0.67
R0454:Map3k21	UTSW	8	126,668,858 (GRCm39)	missense	probably benign
R0654:Map3k21	UTSW	8	126,668,759 (GRCm39)	missense	probably benign	0.07
R1141:Map3k21	UTSW	8	126,668,471 (GRCm39)	missense	probably benign	0.32
R1177:Map3k21	UTSW	8	126,671,577 (GRCm39)	missense	probably benign	0.31
R1463:Map3k21	UTSW	8	126,668,876 (GRCm39)	missense	probably benign	0.00
R1472:Map3k21	UTSW	8	126,668,417 (GRCm39)	missense	probably benign
R1759:Map3k21	UTSW	8	126,671,519 (GRCm39)	missense	probably benign
R1988:Map3k21	UTSW	8	126,654,294 (GRCm39)	missense	probably benign	0.07
R2058:Map3k21	UTSW	8	126,665,461 (GRCm39)	missense	probably benign	0.01
R2117:Map3k21	UTSW	8	126,650,781 (GRCm39)	missense	probably benign	0.19
R2157:Map3k21	UTSW	8	126,664,005 (GRCm39)	missense	probably benign
R2436:Map3k21	UTSW	8	126,668,354 (GRCm39)	nonsense	probably null
R2507:Map3k21	UTSW	8	126,666,677 (GRCm39)	missense	possibly damaging	0.73
R3125:Map3k21	UTSW	8	126,668,593 (GRCm39)	missense	probably benign	0.26
R3746:Map3k21	UTSW	8	126,661,839 (GRCm39)	missense	probably damaging	1.00
R4016:Map3k21	UTSW	8	126,637,924 (GRCm39)	missense	probably damaging	1.00
R4647:Map3k21	UTSW	8	126,668,850 (GRCm39)	missense	probably benign
R4648:Map3k21	UTSW	8	126,668,850 (GRCm39)	missense	probably benign
R4864:Map3k21	UTSW	8	126,654,294 (GRCm39)	missense	probably benign	0.04
R5642:Map3k21	UTSW	8	126,665,563 (GRCm39)	missense	probably benign	0.17
R5694:Map3k21	UTSW	8	126,671,507 (GRCm39)	missense	probably benign	0.04
R5950:Map3k21	UTSW	8	126,668,499 (GRCm39)	missense	possibly damaging	0.93
R5982:Map3k21	UTSW	8	126,638,169 (GRCm39)	missense	probably damaging	1.00
R6440:Map3k21	UTSW	8	126,637,876 (GRCm39)	missense	probably damaging	1.00
R6550:Map3k21	UTSW	8	126,664,031 (GRCm39)	missense	probably damaging	1.00
R6668:Map3k21	UTSW	8	126,652,852 (GRCm39)	missense	possibly damaging	0.60
R6788:Map3k21	UTSW	8	126,666,605 (GRCm39)	missense	probably benign	0.28
R7369:Map3k21	UTSW	8	126,637,855 (GRCm39)	missense	possibly damaging	0.86
R7371:Map3k21	UTSW	8	126,661,804 (GRCm39)	missense	probably damaging	0.99
R7381:Map3k21	UTSW	8	126,671,717 (GRCm39)	missense	possibly damaging	0.83
R7388:Map3k21	UTSW	8	126,654,336 (GRCm39)	missense	probably damaging	1.00
R7397:Map3k21	UTSW	8	126,661,855 (GRCm39)	missense	probably damaging	1.00
R7497:Map3k21	UTSW	8	126,654,340 (GRCm39)	missense	probably damaging	0.99
R7562:Map3k21	UTSW	8	126,665,539 (GRCm39)	missense	probably damaging	1.00
R7564:Map3k21	UTSW	8	126,654,447 (GRCm39)	critical splice donor site	probably null
R7824:Map3k21	UTSW	8	126,637,702 (GRCm39)	missense	probably benign	0.01
R8286:Map3k21	UTSW	8	126,637,498 (GRCm39)	missense	probably benign	0.00
R8351:Map3k21	UTSW	8	126,671,472 (GRCm39)	missense	probably benign	0.00
R8451:Map3k21	UTSW	8	126,671,472 (GRCm39)	missense	probably benign	0.00
R8461:Map3k21	UTSW	8	126,671,361 (GRCm39)	missense	probably benign	0.05
R9005:Map3k21	UTSW	8	126,637,471 (GRCm39)	missense
R9074:Map3k21	UTSW	8	126,664,050 (GRCm39)	missense	probably damaging	0.98
R9156:Map3k21	UTSW	8	126,665,463 (GRCm39)	missense	possibly damaging	0.81
R9217:Map3k21	UTSW	8	126,638,027 (GRCm39)	missense	possibly damaging	0.47
R9474:Map3k21	UTSW	8	126,650,903 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTACCTCTTTGCAAAGACAGCC -3'
(R):5'- GCAGCAAACACTTCATGGAG -3'

Sequencing Primer
(F):5'- CTCATCAGAGGCTGAGAGTCG -3'
(R):5'- CACTTCATGGAGAGAGAAGACTTTG -3'

Posted On

2018-07-23