Mutagenetix > Incidental Mutation

Incidental Mutation 'R6674:Vmn2r88'

527222

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r88

Ensembl Gene

ENSMUSG00000000606

Gene Name

vomeronasal 2, receptor 88

Synonyms

V2r13, V2r3

MMRRC Submission

044794-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R6674 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

51648458-51656984 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 51651795 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 378 (I378V)

Ref Sequence

ENSEMBL: ENSMUSP00000022438 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022438] [ENSMUST00000159674] [ENSMUST00000162998] [ENSMUST00000228139]

AlphaFold

L7N1W8

Predicted Effect

probably benign
Transcript: ENSMUST00000022438
AA Change: I378V

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000022438
Gene: ENSMUSG00000000606
AA Change: I378V

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	457	8.3e-27	PFAM
Pfam:NCD3G	516	570	1.2e-18	PFAM
Pfam:7tm_3	603	838	1.9e-55	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000159674
AA Change: I370V

SMART Domains

Protein: ENSMUSP00000125126
Gene: ENSMUSG00000000606
AA Change: I370V

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	30	408	3.2e-30	PFAM
Pfam:NCD3G	463	516	1.2e-19	PFAM
Pfam:7tm_3	546	785	3.7e-81	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161565

Predicted Effect

probably benign
Transcript: ENSMUST00000162998

SMART Domains

Protein: ENSMUSP00000125409
Gene: ENSMUSG00000068399

Domain	Start	End	E-Value	Type
Pfam:Takusan	35	115	2.2e-25	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000163019
AA Change: I345V

SMART Domains

Protein: ENSMUSP00000124837
Gene: ENSMUSG00000000606
AA Change: I345V

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	52	399	3.7e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000228139
AA Change: I370V

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Armh3	T	C	19: 45,963,437 (GRCm39)	E92G	probably benign	Het
Cluh	A	G	11: 74,557,053 (GRCm39)	H1005R	probably damaging	Het
Coro2b	C	T	9: 62,339,709 (GRCm39)	R13H	probably damaging	Het
Depdc1a	T	A	3: 159,232,344 (GRCm39)	N698K	probably benign	Het
Dusp12	G	A	1: 170,707,317 (GRCm39)	T257I	probably benign	Het
Esf1	T	A	2: 139,962,726 (GRCm39)	K782*	probably null	Het
Extl1	T	C	4: 134,085,438 (GRCm39)	T560A	probably damaging	Het
Gpr179	C	A	11: 97,238,231 (GRCm39)		probably null	Het
Krt90	G	A	15: 101,465,761 (GRCm39)	L287F	probably damaging	Het
Mertk	T	C	2: 128,571,277 (GRCm39)	V77A	probably benign	Het
Nr4a2	C	T	2: 57,002,436 (GRCm39)	A6T	probably damaging	Het
Pdzd8	G	A	19: 59,289,801 (GRCm39)	P533L	probably damaging	Het
Plxnb1	G	A	9: 108,937,214 (GRCm39)	G1132R	probably benign	Het
Ppfia2	A	G	10: 106,763,633 (GRCm39)	I1209V	probably benign	Het
Slc26a9	A	T	1: 131,692,756 (GRCm39)	Q696L	probably benign	Het
Sphkap	A	T	1: 83,255,555 (GRCm39)	S444R	probably benign	Het
Tmem144	A	G	3: 79,746,490 (GRCm39)	F22L	possibly damaging	Het
Tomm20l	A	G	12: 71,158,307 (GRCm39)	D30G	probably damaging	Het
Trpc2	A	G	7: 101,745,264 (GRCm39)	T827A	probably benign	Het
Unc50	T	C	1: 37,476,539 (GRCm39)	V208A	probably benign	Het
Vmn1r125	T	C	7: 21,006,638 (GRCm39)	S179P	probably damaging	Het
Vmn1r225	A	C	17: 20,723,377 (GRCm39)	I273L	probably benign	Het
Vmn2r117	G	A	17: 23,679,023 (GRCm39)	Q734*	probably null	Het
Zfp507	C	T	7: 35,494,159 (GRCm39)	V295I	probably damaging	Het
Zfp981	A	G	4: 146,619,950 (GRCm39)	R35G	probably damaging	Het

Other mutations in Vmn2r88

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00990:Vmn2r88	APN	14	51,650,713 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51,650,517 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51,650,582 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51,654,259 (GRCm39)	missense	possibly damaging	0.59
IGL02308:Vmn2r88	APN	14	51,655,437 (GRCm39)	missense	possibly damaging	0.96
IGL02481:Vmn2r88	APN	14	51,651,611 (GRCm39)	missense	probably benign
IGL02483:Vmn2r88	APN	14	51,651,611 (GRCm39)	missense	probably benign
IGL03241:Vmn2r88	APN	14	51,655,830 (GRCm39)	missense	probably benign	0.03
R0052:Vmn2r88	UTSW	14	51,656,157 (GRCm39)	missense	possibly damaging	0.88
R0070:Vmn2r88	UTSW	14	51,651,597 (GRCm39)	missense	probably benign	0.08
R0799:Vmn2r88	UTSW	14	51,651,959 (GRCm39)	missense	possibly damaging	0.61
R0906:Vmn2r88	UTSW	14	51,655,666 (GRCm39)	missense	probably damaging	1.00
R1322:Vmn2r88	UTSW	14	51,651,565 (GRCm39)	missense	probably damaging	1.00
R1352:Vmn2r88	UTSW	14	51,656,007 (GRCm39)	missense	probably damaging	1.00
R1639:Vmn2r88	UTSW	14	51,654,244 (GRCm39)	missense	probably damaging	0.98
R1780:Vmn2r88	UTSW	14	51,656,029 (GRCm39)	missense	probably damaging	1.00
R1834:Vmn2r88	UTSW	14	51,650,487 (GRCm39)	splice site	probably benign
R1911:Vmn2r88	UTSW	14	51,655,671 (GRCm39)	missense	probably damaging	1.00
R2113:Vmn2r88	UTSW	14	51,655,651 (GRCm39)	missense	probably damaging	1.00
R2120:Vmn2r88	UTSW	14	51,650,665 (GRCm39)	missense	probably benign	0.00
R2126:Vmn2r88	UTSW	14	51,651,264 (GRCm39)	missense	probably benign	0.01
R2348:Vmn2r88	UTSW	14	51,651,461 (GRCm39)	missense	probably benign	0.00
R2881:Vmn2r88	UTSW	14	51,656,146 (GRCm39)	missense	probably damaging	0.97
R2884:Vmn2r88	UTSW	14	51,651,391 (GRCm39)	missense	probably damaging	1.00
R3081:Vmn2r88	UTSW	14	51,656,089 (GRCm39)	missense	probably damaging	0.99
R3933:Vmn2r88	UTSW	14	51,651,435 (GRCm39)	missense	probably benign	0.44
R3967:Vmn2r88	UTSW	14	51,650,647 (GRCm39)	missense	probably benign	0.06
R4091:Vmn2r88	UTSW	14	51,652,883 (GRCm39)	missense	probably damaging	1.00
R4378:Vmn2r88	UTSW	14	51,650,746 (GRCm39)	nonsense	probably null
R4397:Vmn2r88	UTSW	14	51,655,435 (GRCm39)	missense	probably damaging	1.00
R4418:Vmn2r88	UTSW	14	51,655,538 (GRCm39)	missense	probably damaging	1.00
R4609:Vmn2r88	UTSW	14	51,655,531 (GRCm39)	missense	probably damaging	0.98
R4647:Vmn2r88	UTSW	14	51,656,250 (GRCm39)	missense	probably benign	0.02
R4672:Vmn2r88	UTSW	14	51,655,612 (GRCm39)	missense	probably damaging	1.00
R4684:Vmn2r88	UTSW	14	51,650,791 (GRCm39)	missense	possibly damaging	0.95
R4686:Vmn2r88	UTSW	14	51,650,796 (GRCm39)	missense	probably benign	0.03
R4720:Vmn2r88	UTSW	14	51,650,702 (GRCm39)	missense	probably benign	0.01
R5046:Vmn2r88	UTSW	14	51,650,638 (GRCm39)	missense	probably benign	0.03
R5063:Vmn2r88	UTSW	14	51,648,603 (GRCm39)	missense	probably damaging	0.96
R5619:Vmn2r88	UTSW	14	51,651,367 (GRCm39)	missense	probably damaging	0.99
R5652:Vmn2r88	UTSW	14	51,656,029 (GRCm39)	missense	probably damaging	0.98
R6020:Vmn2r88	UTSW	14	51,655,606 (GRCm39)	nonsense	probably null
R6103:Vmn2r88	UTSW	14	51,652,826 (GRCm39)	missense	probably benign	0.17
R6799:Vmn2r88	UTSW	14	51,651,426 (GRCm39)	missense	probably benign	0.05
R7089:Vmn2r88	UTSW	14	51,656,100 (GRCm39)	missense
R7104:Vmn2r88	UTSW	14	51,651,253 (GRCm39)	missense
R7265:Vmn2r88	UTSW	14	51,655,776 (GRCm39)	missense
R7316:Vmn2r88	UTSW	14	51,651,712 (GRCm39)	missense
R7552:Vmn2r88	UTSW	14	51,648,315 (GRCm39)	splice site	probably null
R7611:Vmn2r88	UTSW	14	51,651,454 (GRCm39)	missense
R7667:Vmn2r88	UTSW	14	51,655,446 (GRCm39)	missense
R7682:Vmn2r88	UTSW	14	51,655,906 (GRCm39)	missense
R7755:Vmn2r88	UTSW	14	51,650,503 (GRCm39)	missense	probably benign	0.00
R7811:Vmn2r88	UTSW	14	51,656,160 (GRCm39)	missense
R7882:Vmn2r88	UTSW	14	51,650,503 (GRCm39)	missense	probably benign	0.00
R7957:Vmn2r88	UTSW	14	51,650,589 (GRCm39)	missense
R7998:Vmn2r88	UTSW	14	51,651,565 (GRCm39)	missense
R8142:Vmn2r88	UTSW	14	51,651,564 (GRCm39)	missense
R8186:Vmn2r88	UTSW	14	51,656,157 (GRCm39)	missense
R8348:Vmn2r88	UTSW	14	51,656,253 (GRCm39)	missense	probably damaging	0.97
R8448:Vmn2r88	UTSW	14	51,656,253 (GRCm39)	missense	probably damaging	0.97
R8483:Vmn2r88	UTSW	14	51,650,530 (GRCm39)	missense	possibly damaging	0.48
R8783:Vmn2r88	UTSW	14	51,651,523 (GRCm39)	missense
R8859:Vmn2r88	UTSW	14	51,656,263 (GRCm39)	missense	probably damaging	0.97
R8916:Vmn2r88	UTSW	14	51,648,593 (GRCm39)	missense
R8936:Vmn2r88	UTSW	14	51,655,983 (GRCm39)	missense	possibly damaging	0.88
R9004:Vmn2r88	UTSW	14	51,650,624 (GRCm39)	missense
R9038:Vmn2r88	UTSW	14	51,651,490 (GRCm39)	missense
R9063:Vmn2r88	UTSW	14	51,648,329 (GRCm39)	start gained	probably benign
R9311:Vmn2r88	UTSW	14	51,650,503 (GRCm39)	missense	probably benign	0.00
R9382:Vmn2r88	UTSW	14	51,656,197 (GRCm39)	missense
R9483:Vmn2r88	UTSW	14	51,648,641 (GRCm39)	missense
R9602:Vmn2r88	UTSW	14	51,651,189 (GRCm39)	missense
V5622:Vmn2r88	UTSW	14	51,650,584 (GRCm39)	missense	probably benign
X0024:Vmn2r88	UTSW	14	51,651,289 (GRCm39)	missense	possibly damaging	0.79
X0025:Vmn2r88	UTSW	14	51,654,259 (GRCm39)	missense	possibly damaging	0.59
Z1177:Vmn2r88	UTSW	14	51,655,644 (GRCm39)	missense
Z1177:Vmn2r88	UTSW	14	51,655,503 (GRCm39)	frame shift	probably null
Z1190:Vmn2r88	UTSW	14	51,650,658 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACAACCTCACAATGGGATGTC -3'
(R):5'- GTGTAGAAATCGTTGCTCTGAG -3'

Sequencing Primer
(F):5'- CCTCACAATGGGATGTCATCAC -3'
(R):5'- GAAATCGTTGCTCTGAGAGAATAATC -3'

Posted On

2018-07-23