Incidental Mutation 'R6703:Psg16'
ID528282
Institutional Source Beutler Lab
Gene Symbol Psg16
Ensembl Gene ENSMUSG00000066760
Gene Namepregnancy specific glycoprotein 16
SynonymsbCEA, Cea11
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.054) question?
Stock #R6703 (G1)
Quality Score225.009
Status Validated
Chromosome7
Chromosomal Location17074040-17133450 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 17090396 bp
ZygosityHeterozygous
Amino Acid Change Leucine to Proline at position 35 (L35P)
Ref Sequence ENSEMBL: ENSMUSP00000118977 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071399] [ENSMUST00000118367] [ENSMUST00000152671]
Predicted Effect probably benign
Transcript: ENSMUST00000071399
SMART Domains Protein: ENSMUSP00000071348
Gene: ENSMUSG00000066760

DomainStartEndE-ValueType
IG_like 6 52 1.42e2 SMART
IG 71 172 1.21e-2 SMART
IG 191 292 2.56e-1 SMART
IG_like 302 395 5.13e0 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000118367
SMART Domains Protein: ENSMUSP00000113025
Gene: ENSMUSG00000066760

DomainStartEndE-ValueType
IG_like 6 52 1.42e2 SMART
IG 71 172 1.21e-2 SMART
IG 191 292 2.56e-1 SMART
IGc2 308 372 3.56e-9 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000131153
Predicted Effect probably damaging
Transcript: ENSMUST00000152671
AA Change: L35P

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000118977
Gene: ENSMUSG00000066760
AA Change: L35P

DomainStartEndE-ValueType
low complexity region 24 40 N/A INTRINSIC
IG 46 143 4.29e-3 SMART
IG 162 261 2.94e-1 SMART
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.3%
  • 20x: 95.0%
Validation Efficiency 99% (73/74)
Allele List at MGI
Other mutations in this stock
Total: 71 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700020N01Rik T A 10: 21,621,659 Y66* probably null Het
1700080E11Rik T C 9: 105,144,559 Y96C possibly damaging Het
Akap13 T C 7: 75,602,898 S259P probably damaging Het
Aoc1 A C 6: 48,905,714 T197P probably damaging Het
Blnk A T 19: 40,962,506 probably null Het
Ccser1 A T 6: 61,638,511 K94* probably null Het
Cfap54 T A 10: 92,868,734 D2828V unknown Het
Clec4n A G 6: 123,235,594 Q89R probably null Het
Col6a3 T C 1: 90,779,439 D1984G unknown Het
Col6a3 C T 1: 90,792,462 R1552Q probably benign Het
Csnk2a1 A G 2: 152,258,688 T93A probably benign Het
Dna2 T A 10: 62,973,294 I1055N possibly damaging Het
Dnm3 A G 1: 162,318,687 F296L probably benign Het
Dock7 T C 4: 98,946,672 E1822G probably damaging Het
Dpyd A T 3: 118,897,200 probably null Het
Dyrk4 A T 6: 126,890,082 I329N probably damaging Het
E4f1 C A 17: 24,447,131 R231L probably damaging Het
Fat1 A G 8: 44,953,046 T945A probably benign Het
Fkbp7 A T 2: 76,671,762 M99K probably damaging Het
Flad1 T C 3: 89,408,590 S222G probably benign Het
H1fnt C A 15: 98,257,272 probably benign Het
Ighg2b T C 12: 113,305,033 probably benign Het
Iqub T A 6: 24,449,745 N707I probably damaging Het
Itln1 C T 1: 171,530,583 C199Y probably damaging Het
Kctd3 G A 1: 188,996,529 R137C probably damaging Het
Lamp5 C G 2: 136,059,563 N102K possibly damaging Het
Larp7 C T 3: 127,544,224 M395I probably damaging Het
Lpcat3 C T 6: 124,663,222 A5V probably benign Het
Lrrc43 C T 5: 123,499,469 T233M possibly damaging Het
Map4k1 T G 7: 29,002,396 S803A possibly damaging Het
Mef2c T A 13: 83,625,406 C134S possibly damaging Het
Mgst2 T C 3: 51,664,612 probably null Het
Mug2 T G 6: 122,078,694 I1112R probably benign Het
Myo15 A G 11: 60,492,992 I1622V probably benign Het
Ndor1 A G 2: 25,249,890 F142S possibly damaging Het
Nectin3 A G 16: 46,463,842 S160P probably damaging Het
Nynrin A G 14: 55,864,478 T535A possibly damaging Het
Olfr1290 T A 2: 111,490,109 probably null Het
Olfr165 T C 16: 19,407,372 I215V probably benign Het
Olfr727 A G 14: 50,127,231 Y218C probably damaging Het
Olfr916 A T 9: 38,657,777 I205N possibly damaging Het
Pcdh10 G A 3: 45,381,299 V683M possibly damaging Het
Per2 C T 1: 91,427,949 E696K probably damaging Het
Pif1 T C 9: 65,593,263 V490A probably damaging Het
Plekhn1 A T 4: 156,224,793 Y219N probably benign Het
Ppl T G 16: 5,089,464 E989A probably damaging Het
Prkdc T G 16: 15,670,528 S505A probably benign Het
Ptges A G 2: 30,903,121 V33A possibly damaging Het
Qser1 G A 2: 104,777,325 T1416I possibly damaging Het
Rab3a A G 8: 70,756,448 D77G probably damaging Het
Rtn3 A T 19: 7,435,045 V788D probably damaging Het
Rtn4r T C 16: 18,151,191 L161P probably damaging Het
Rtn4rl1 G A 11: 75,265,528 R262Q probably benign Het
S1pr3 A T 13: 51,419,439 I219F probably damaging Het
Sec23b A T 2: 144,559,189 probably null Het
Senp6 A G 9: 80,121,921 E522G probably damaging Het
Slc27a6 A G 18: 58,609,839 N533S probably benign Het
Slc39a5 T A 10: 128,397,782 D282V probably damaging Het
Smc1b C T 15: 85,092,031 R825Q probably benign Het
Snx14 A G 9: 88,422,914 I109T probably damaging Het
Sorl1 A T 9: 42,071,201 V361E probably damaging Het
Sox5 G T 6: 143,833,465 S648R probably damaging Het
Sptbn2 G A 19: 4,749,814 S2161N probably benign Het
Sptbn2 C A 19: 4,749,815 S2161R probably benign Het
St6galnac2 A G 11: 116,684,387 S209P probably benign Het
Tmub1 T C 5: 24,446,946 S7G probably benign Het
Trpm5 C A 7: 143,069,318 probably benign Het
Tusc5 A T 11: 76,694,162 probably null Het
Vmn2r106 C T 17: 20,268,463 C558Y probably damaging Het
Zfp438 A G 18: 5,214,044 S305P probably benign Het
Zfp780b T C 7: 27,971,641 T81A possibly damaging Het
Other mutations in Psg16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01993:Psg16 APN 7 17093706 missense probably benign 0.08
IGL02258:Psg16 APN 7 17095292 missense probably damaging 1.00
R0379:Psg16 UTSW 7 17130658 missense probably benign 0.09
R0389:Psg16 UTSW 7 17095163 missense probably benign
R0443:Psg16 UTSW 7 17095163 missense probably benign
R1231:Psg16 UTSW 7 17095305 nonsense probably null
R1594:Psg16 UTSW 7 17093823 missense probably damaging 1.00
R2064:Psg16 UTSW 7 17093748 missense possibly damaging 0.91
R2118:Psg16 UTSW 7 17090623 missense probably benign 0.33
R3806:Psg16 UTSW 7 17090684 missense probably benign 0.24
R4397:Psg16 UTSW 7 17090698 missense possibly damaging 0.68
R4583:Psg16 UTSW 7 17095172 missense probably benign 0.01
R4685:Psg16 UTSW 7 17090534 missense probably benign 0.00
R4929:Psg16 UTSW 7 17095106 missense possibly damaging 0.79
R5310:Psg16 UTSW 7 17090635 missense probably damaging 0.99
R6106:Psg16 UTSW 7 17095166 missense possibly damaging 0.73
R6320:Psg16 UTSW 7 17088187 missense probably damaging 1.00
R6702:Psg16 UTSW 7 17090396 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GTGCTGATCTCTGTAGAGGAGT -3'
(R):5'- TGCCCCATCACACTTACTTTAGT -3'

Sequencing Primer
(F):5'- AACCACAGTTTCTGGTCAGG -3'
(R):5'- ACTTTAGTGTTCAGTGTGTATACAAC -3'
Posted On2018-07-24