Incidental Mutation 'R6711:Ankrd35'
ID 529121
Institutional Source Beutler Lab
Gene Symbol Ankrd35
Ensembl Gene ENSMUSG00000038354
Gene Name ankyrin repeat domain 35
Synonyms 4732436F15Rik
MMRRC Submission 044829-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6711 (G1)
Quality Score 225.009
Status Validated
Chromosome 3
Chromosomal Location 96577447-96598348 bp(+) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) C to T at 96590784 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamine to Stop codon at position 357 (Q357*)
Ref Sequence ENSEMBL: ENSMUSP00000047244 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000048427] [ENSMUST00000122960]
AlphaFold E9Q9D8
Predicted Effect probably null
Transcript: ENSMUST00000048427
AA Change: Q357*
SMART Domains Protein: ENSMUSP00000047244
Gene: ENSMUSG00000038354
AA Change: Q357*

DomainStartEndE-ValueType
ANK 53 82 4.03e-5 SMART
ANK 86 115 6.46e-4 SMART
ANK 119 148 4.36e-1 SMART
ANK 152 181 1.4e-4 SMART
ANK 185 214 2.25e-3 SMART
ANK 218 247 6.24e2 SMART
coiled coil region 294 339 N/A INTRINSIC
low complexity region 438 455 N/A INTRINSIC
low complexity region 487 498 N/A INTRINSIC
low complexity region 524 536 N/A INTRINSIC
coiled coil region 606 653 N/A INTRINSIC
coiled coil region 729 799 N/A INTRINSIC
low complexity region 815 829 N/A INTRINSIC
coiled coil region 847 956 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000122960
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130429
Meta Mutation Damage Score 0.9699 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.5%
Validation Efficiency 100% (47/47)
Allele List at MGI
Other mutations in this stock
Total: 46 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Akr1c21 A G 13: 4,627,374 (GRCm39) D156G probably damaging Het
Ano2 G A 6: 125,752,795 (GRCm39) A191T probably damaging Het
Cacna2d1 C T 5: 16,505,039 (GRCm39) T331I probably damaging Het
Ccdc81 T G 7: 89,537,006 (GRCm39) E214A probably damaging Het
Cdh4 A G 2: 179,532,724 (GRCm39) T729A probably damaging Het
Ceacam2 A G 7: 25,238,295 (GRCm39) L43P probably benign Het
Ces2h A T 8: 105,744,715 (GRCm39) R364S probably benign Het
Dcpp3 AGGCCATGCTGGCC AGGCC 17: 24,136,572 (GRCm39) probably benign Het
Entrep1 T C 19: 23,955,463 (GRCm39) N385S probably benign Het
Epb42 T A 2: 120,854,589 (GRCm39) probably benign Het
Fcgbp G A 7: 27,789,098 (GRCm39) V555M probably damaging Het
Ganc A T 2: 120,281,320 (GRCm39) H723L possibly damaging Het
Gfra2 A G 14: 71,203,715 (GRCm39) D31G probably damaging Het
Glrb A G 3: 80,752,281 (GRCm39) I443T probably benign Het
Hcfc1 A T X: 72,993,671 (GRCm39) C1165S probably damaging Homo
Hoxd10 A G 2: 74,524,507 (GRCm39) Y273C probably damaging Het
Iba57 T C 11: 59,049,369 (GRCm39) T267A probably damaging Het
Impg2 C A 16: 56,085,449 (GRCm39) P943H probably damaging Het
Kidins220 A G 12: 25,048,750 (GRCm39) T145A probably damaging Het
Lmntd1 T C 6: 145,489,228 (GRCm39) Y11C probably benign Het
Lyst A T 13: 13,809,820 (GRCm39) T497S possibly damaging Het
Man1a G A 10: 53,809,588 (GRCm39) H406Y probably benign Het
Mme A T 3: 63,249,339 (GRCm39) K289N possibly damaging Het
Mrps21 G A 3: 95,777,895 (GRCm39) probably benign Het
Mtor A G 4: 148,536,824 (GRCm39) N33D possibly damaging Het
Ncoa1 C A 12: 4,372,904 (GRCm39) A166S probably benign Het
Ndrg2 A T 14: 52,147,782 (GRCm39) F112I possibly damaging Het
Neb A C 2: 52,113,076 (GRCm39) F36C probably benign Het
Neb A G 2: 52,146,299 (GRCm39) Y2893H probably damaging Het
Or8g22 A G 9: 38,958,162 (GRCm39) *229Q probably null Het
Pcdh15 A T 10: 74,478,219 (GRCm39) E231D possibly damaging Het
Pih1d2 T A 9: 50,529,310 (GRCm39) M1K probably null Het
Pla2g4e T C 2: 120,001,751 (GRCm39) N633D probably benign Het
Rph3al C A 11: 75,799,810 (GRCm39) G50* probably null Het
Rtcb A T 10: 85,774,963 (GRCm39) N477K possibly damaging Het
Scn10a A G 9: 119,438,979 (GRCm39) F1630S probably damaging Het
Srfbp1 T C 18: 52,621,373 (GRCm39) S145P probably damaging Het
St7 A G 6: 17,848,069 (GRCm39) E211G possibly damaging Het
Thbs2 T C 17: 14,910,527 (GRCm39) D24G probably benign Het
Tlr6 C A 5: 65,111,835 (GRCm39) M357I probably damaging Het
Tmem168 T C 6: 13,603,120 (GRCm39) Y82C probably damaging Het
Tnrc18 A T 5: 142,773,545 (GRCm39) L245Q unknown Het
Vmn1r8 T C 6: 57,013,444 (GRCm39) L165P probably damaging Het
Vps13b A G 15: 35,887,395 (GRCm39) Y3268C probably damaging Het
Zdhhc7 A G 8: 120,810,066 (GRCm39) I218T probably benign Het
Zfp213 A G 17: 23,778,485 (GRCm39) F209S probably benign Het
Other mutations in Ankrd35
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00819:Ankrd35 APN 3 96,590,350 (GRCm39) splice site probably null
IGL00896:Ankrd35 APN 3 96,591,592 (GRCm39) missense probably damaging 1.00
IGL01565:Ankrd35 APN 3 96,592,101 (GRCm39) missense probably damaging 0.99
IGL01837:Ankrd35 APN 3 96,587,982 (GRCm39) missense probably damaging 1.00
IGL02605:Ankrd35 APN 3 96,588,388 (GRCm39) splice site probably null
IGL02819:Ankrd35 APN 3 96,597,524 (GRCm39) missense possibly damaging 0.80
IGL02994:Ankrd35 APN 3 96,590,307 (GRCm39) splice site probably benign
IGL03083:Ankrd35 APN 3 96,592,117 (GRCm39) missense probably damaging 1.00
IGL03105:Ankrd35 APN 3 96,591,373 (GRCm39) missense probably benign
FR4304:Ankrd35 UTSW 3 96,591,163 (GRCm39) utr 3 prime probably benign
FR4342:Ankrd35 UTSW 3 96,590,831 (GRCm39) frame shift probably null
FR4737:Ankrd35 UTSW 3 96,591,165 (GRCm39) utr 3 prime probably benign
R0003:Ankrd35 UTSW 3 96,591,331 (GRCm39) missense probably damaging 1.00
R0047:Ankrd35 UTSW 3 96,591,379 (GRCm39) missense probably benign 0.00
R0551:Ankrd35 UTSW 3 96,591,276 (GRCm39) missense probably benign 0.08
R1420:Ankrd35 UTSW 3 96,592,054 (GRCm39) missense probably benign 0.13
R1455:Ankrd35 UTSW 3 96,585,471 (GRCm39) missense probably damaging 1.00
R2201:Ankrd35 UTSW 3 96,586,564 (GRCm39) missense possibly damaging 0.93
R3522:Ankrd35 UTSW 3 96,592,378 (GRCm39) missense probably damaging 1.00
R3605:Ankrd35 UTSW 3 96,589,497 (GRCm39) nonsense probably null
R4166:Ankrd35 UTSW 3 96,586,471 (GRCm39) splice site probably null
R4651:Ankrd35 UTSW 3 96,591,343 (GRCm39) missense probably benign 0.00
R4668:Ankrd35 UTSW 3 96,586,524 (GRCm39) missense probably damaging 1.00
R4916:Ankrd35 UTSW 3 96,591,438 (GRCm39) missense probably benign
R4921:Ankrd35 UTSW 3 96,592,140 (GRCm39) missense possibly damaging 0.61
R4953:Ankrd35 UTSW 3 96,590,989 (GRCm39) missense possibly damaging 0.56
R5180:Ankrd35 UTSW 3 96,587,789 (GRCm39) missense probably damaging 1.00
R5583:Ankrd35 UTSW 3 96,592,219 (GRCm39) missense probably damaging 1.00
R5604:Ankrd35 UTSW 3 96,592,215 (GRCm39) missense probably benign 0.02
R5613:Ankrd35 UTSW 3 96,590,334 (GRCm39) missense possibly damaging 0.76
R6165:Ankrd35 UTSW 3 96,590,623 (GRCm39) missense possibly damaging 0.93
R6413:Ankrd35 UTSW 3 96,592,129 (GRCm39) missense probably damaging 0.96
R6834:Ankrd35 UTSW 3 96,590,599 (GRCm39) missense possibly damaging 0.68
R6841:Ankrd35 UTSW 3 96,577,742 (GRCm39) missense probably damaging 1.00
R7028:Ankrd35 UTSW 3 96,590,650 (GRCm39) missense possibly damaging 0.92
R7396:Ankrd35 UTSW 3 96,590,813 (GRCm39) missense probably damaging 1.00
R7425:Ankrd35 UTSW 3 96,592,104 (GRCm39) missense not run
R7815:Ankrd35 UTSW 3 96,592,117 (GRCm39) missense probably damaging 1.00
R7887:Ankrd35 UTSW 3 96,592,216 (GRCm39) missense probably damaging 1.00
R8103:Ankrd35 UTSW 3 96,586,997 (GRCm39) missense possibly damaging 0.93
R8318:Ankrd35 UTSW 3 96,592,038 (GRCm39) missense probably damaging 1.00
R8492:Ankrd35 UTSW 3 96,589,529 (GRCm39) critical splice donor site probably null
R8527:Ankrd35 UTSW 3 96,589,353 (GRCm39) missense probably damaging 1.00
R8542:Ankrd35 UTSW 3 96,589,353 (GRCm39) missense probably damaging 1.00
R8742:Ankrd35 UTSW 3 96,586,502 (GRCm39) missense probably damaging 1.00
R8963:Ankrd35 UTSW 3 96,587,003 (GRCm39) nonsense probably null
R9029:Ankrd35 UTSW 3 96,591,460 (GRCm39) missense probably benign 0.43
R9229:Ankrd35 UTSW 3 96,592,215 (GRCm39) missense probably benign 0.02
R9488:Ankrd35 UTSW 3 96,589,343 (GRCm39) missense probably damaging 1.00
R9669:Ankrd35 UTSW 3 96,587,797 (GRCm39) missense probably damaging 1.00
R9761:Ankrd35 UTSW 3 96,591,733 (GRCm39) missense possibly damaging 0.80
Z1177:Ankrd35 UTSW 3 96,591,086 (GRCm39) missense probably damaging 0.96
Predicted Primers PCR Primer
(F):5'- ATCTCCTCCTAAGAACTCACGG -3'
(R):5'- CTTCATGATGGATCTCAGCCG -3'

Sequencing Primer
(F):5'- GCGGTGCTCAGAAGAGTG -3'
(R):5'- GCCGGGGCTAATTCTTCAG -3'
Posted On 2018-07-24