Mutagenetix > Incidental Mutation

Incidental Mutation 'R6741:Zmym4'

530440

Institutional Source

Beutler Lab

Gene Symbol

Zmym4

Ensembl Gene

ENSMUSG00000042446

Gene Name

zinc finger, MYM-type 4

Synonyms

Zfp262, 6330503C17Rik

MMRRC Submission

044858-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.586) question?

Stock #

R6741 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

126755732-126861928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 126808878 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 390 (S390P)

Ref Sequence

ENSEMBL: ENSMUSP00000101714 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106108]

AlphaFold

A2A791

Predicted Effect

possibly damaging
Transcript: ENSMUST00000106108
AA Change: S390P

PolyPhen 2 Score 0.796 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000101714
Gene: ENSMUSG00000042446
AA Change: S390P

Domain	Start	End	E-Value	Type
TRASH	341	377	6.53e-4	SMART
TRASH	389	429	7.22e-6	SMART
TRASH	441	479	1.77e0	SMART
TRASH	486	525	4.95e-4	SMART
TRASH	531	569	1.05e-2	SMART
TRASH	579	615	2.82e1	SMART
low complexity region	640	649	N/A	INTRINSIC
TRASH	687	723	8.49e-3	SMART
TRASH	729	764	1.14e-3	SMART
TRASH	772	810	4.48e-2	SMART
TRASH	816	851	2.06e-1	SMART
low complexity region	974	993	N/A	INTRINSIC
low complexity region	1002	1021	N/A	INTRINSIC
Pfam:DUF3504	1357	1527	1.7e-68	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000135003
AA Change: S138P

SMART Domains

Protein: ENSMUSP00000120813
Gene: ENSMUSG00000042446
AA Change: S138P

Domain	Start	End	E-Value	Type
TRASH	90	126	6.53e-4	SMART
TRASH	138	178	7.22e-6	SMART
TRASH	190	228	1.77e0	SMART
TRASH	235	274	3.05e-4	SMART
low complexity region	300	309	N/A	INTRINSIC
TRASH	347	383	8.49e-3	SMART
TRASH	389	424	1.14e-3	SMART
TRASH	432	470	4.48e-2	SMART
TRASH	476	511	2.06e-1	SMART
low complexity region	634	653	N/A	INTRINSIC
low complexity region	662	681	N/A	INTRINSIC
Pfam:DUF3504	1017	1187	1.5e-68	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151026

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152489

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152952

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.0%

Validation Efficiency

97% (34/35)

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actrt3	A	C	3: 30,652,663 (GRCm39)	F144V	possibly damaging	Het
Arhgef12	A	G	9: 42,883,503 (GRCm39)	I1342T	probably benign	Het
Dbn1	A	G	13: 55,629,350 (GRCm39)		probably null	Het
Defb12	T	C	8: 19,164,757 (GRCm39)	E27G	probably benign	Het
Dennd2b	A	G	7: 109,144,304 (GRCm39)	Y534H	possibly damaging	Het
Dthd1	A	T	5: 63,000,289 (GRCm39)	H537L	probably damaging	Het
Ep400	A	T	5: 110,824,761 (GRCm39)	S2359T	unknown	Het
Epha5	T	C	5: 84,254,557 (GRCm39)	I572V	possibly damaging	Het
Grb10	C	A	11: 11,886,717 (GRCm39)		probably null	Het
Hectd2	T	C	19: 36,589,759 (GRCm39)	I628T	probably damaging	Het
Il17rb	T	A	14: 29,722,293 (GRCm39)	Q246L	possibly damaging	Het
Knop1	C	T	7: 118,445,061 (GRCm39)	R301Q	possibly damaging	Het
Lrp1b	T	C	2: 41,136,001 (GRCm39)	I1770M	possibly damaging	Het
Mgst1	A	C	6: 138,127,836 (GRCm39)	D66A	probably damaging	Het
Nags	A	G	11: 102,037,718 (GRCm39)	D237G	possibly damaging	Het
Nfatc2ip	T	A	7: 125,995,182 (GRCm39)	Q122L	possibly damaging	Het
Nod2	T	C	8: 89,379,694 (GRCm39)	V72A	probably damaging	Het
Notch3	G	T	17: 32,362,458 (GRCm39)	P1389Q	probably benign	Het
Or5ac25	A	G	16: 59,181,918 (GRCm39)	L221P	probably damaging	Het
Plekhn1	A	T	4: 156,306,249 (GRCm39)	I607N	probably damaging	Het
Plekho2	C	A	9: 65,471,197 (GRCm39)	R84L	probably damaging	Het
Prag1	A	T	8: 36,614,434 (GRCm39)	M1329L	probably benign	Het
Prpsap2	C	T	11: 61,631,771 (GRCm39)		probably null	Het
Ptprf	A	G	4: 118,080,565 (GRCm39)	S1230P	probably benign	Het
Rigi	A	G	4: 40,211,624 (GRCm39)	I648T	probably damaging	Het
Rptor	T	G	11: 119,786,803 (GRCm39)	L1256R	possibly damaging	Het
Sesn3	T	C	9: 14,231,636 (GRCm39)	I189T	possibly damaging	Het
Skic2	G	A	17: 35,064,166 (GRCm39)	R507*	probably null	Het
Slc39a4	C	T	15: 76,498,283 (GRCm39)	D385N	probably damaging	Het
Tnfsf13b	T	C	8: 10,057,314 (GRCm39)	F128S	possibly damaging	Het
Vmn1r49	A	G	6: 90,049,195 (GRCm39)	V269A	probably benign	Het
Vmn2r69	T	A	7: 85,061,724 (GRCm39)	E83D	probably benign	Het
Vmn2r76	T	C	7: 85,879,560 (GRCm39)	N247D	probably benign	Het

Other mutations in Zmym4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00537:Zmym4	APN	4	126,783,851 (GRCm39)	missense	probably benign
IGL00845:Zmym4	APN	4	126,794,406 (GRCm39)	missense	probably benign	0.28
IGL01122:Zmym4	APN	4	126,758,045 (GRCm39)	missense	probably damaging	1.00
IGL01374:Zmym4	APN	4	126,762,750 (GRCm39)	missense	probably damaging	1.00
IGL01564:Zmym4	APN	4	126,805,073 (GRCm39)	missense	possibly damaging	0.84
IGL02014:Zmym4	APN	4	126,794,462 (GRCm39)	missense	possibly damaging	0.67
IGL02187:Zmym4	APN	4	126,764,066 (GRCm39)	missense	probably damaging	0.97
IGL02887:Zmym4	APN	4	126,842,268 (GRCm39)	missense	probably damaging	0.96
IGL03371:Zmym4	APN	4	126,808,881 (GRCm39)	missense	possibly damaging	0.90
IGL03400:Zmym4	APN	4	126,816,920 (GRCm39)	missense	probably benign	0.12
arriba	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
foreclosed	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
Foreshortened	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
levantese	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
terminated	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
BB004:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
BB014:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R0149:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0361:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0423:Zmym4	UTSW	4	126,776,112 (GRCm39)	splice site	probably benign
R0532:Zmym4	UTSW	4	126,792,194 (GRCm39)	nonsense	probably null
R0745:Zmym4	UTSW	4	126,796,496 (GRCm39)	splice site	probably benign
R1183:Zmym4	UTSW	4	126,819,632 (GRCm39)	missense	probably damaging	0.99
R1401:Zmym4	UTSW	4	126,804,962 (GRCm39)	missense	probably benign	0.00
R1446:Zmym4	UTSW	4	126,776,275 (GRCm39)	missense	probably damaging	1.00
R1491:Zmym4	UTSW	4	126,776,105 (GRCm39)	critical splice acceptor site	probably null
R1566:Zmym4	UTSW	4	126,804,940 (GRCm39)	missense	possibly damaging	0.94
R1962:Zmym4	UTSW	4	126,796,463 (GRCm39)	missense	possibly damaging	0.47
R2398:Zmym4	UTSW	4	126,816,929 (GRCm39)	missense	probably damaging	1.00
R2930:Zmym4	UTSW	4	126,819,316 (GRCm39)	missense	probably benign	0.00
R3891:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R3892:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R4659:Zmym4	UTSW	4	126,842,221 (GRCm39)	splice site	probably null
R4702:Zmym4	UTSW	4	126,816,958 (GRCm39)	missense	probably benign	0.01
R5160:Zmym4	UTSW	4	126,763,977 (GRCm39)	missense	probably damaging	0.97
R5614:Zmym4	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
R5773:Zmym4	UTSW	4	126,799,163 (GRCm39)	missense	possibly damaging	0.52
R6450:Zmym4	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
R7023:Zmym4	UTSW	4	126,762,593 (GRCm39)	missense	probably damaging	1.00
R7315:Zmym4	UTSW	4	126,776,385 (GRCm39)	missense	probably benign	0.09
R7468:Zmym4	UTSW	4	126,776,029 (GRCm39)	missense	probably benign	0.40
R7546:Zmym4	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
R7609:Zmym4	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
R7764:Zmym4	UTSW	4	126,819,409 (GRCm39)	missense	probably benign	0.06
R7897:Zmym4	UTSW	4	126,783,332 (GRCm39)	missense	possibly damaging	0.76
R7918:Zmym4	UTSW	4	126,804,797 (GRCm39)	critical splice acceptor site	probably null
R7927:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R8129:Zmym4	UTSW	4	126,808,956 (GRCm39)	missense	possibly damaging	0.87
R8240:Zmym4	UTSW	4	126,798,188 (GRCm39)	critical splice donor site	probably null
R8248:Zmym4	UTSW	4	126,799,162 (GRCm39)	missense	possibly damaging	0.56
R8261:Zmym4	UTSW	4	126,798,360 (GRCm39)	missense	probably damaging	1.00
R8313:Zmym4	UTSW	4	126,804,762 (GRCm39)	missense	probably benign	0.19
R8353:Zmym4	UTSW	4	126,800,905 (GRCm39)	missense	possibly damaging	0.46
R8747:Zmym4	UTSW	4	126,787,198 (GRCm39)	missense	probably damaging	1.00
R8787:Zmym4	UTSW	4	126,816,953 (GRCm39)	missense	probably benign	0.41
R8795:Zmym4	UTSW	4	126,799,819 (GRCm39)	missense	probably benign	0.35
R8948:Zmym4	UTSW	4	126,758,060 (GRCm39)	missense	probably damaging	1.00
R9218:Zmym4	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
R9233:Zmym4	UTSW	4	126,776,310 (GRCm39)	missense	probably damaging	0.99
R9286:Zmym4	UTSW	4	126,783,812 (GRCm39)	missense	probably damaging	1.00
R9468:Zmym4	UTSW	4	126,800,993 (GRCm39)	missense	probably benign	0.01
R9542:Zmym4	UTSW	4	126,799,164 (GRCm39)	missense	probably benign	0.00
R9756:Zmym4	UTSW	4	126,771,502 (GRCm39)	missense	probably damaging	1.00
R9776:Zmym4	UTSW	4	126,804,942 (GRCm39)	missense	possibly damaging	0.51
U24488:Zmym4	UTSW	4	126,819,453 (GRCm39)	missense	possibly damaging	0.69
Z1177:Zmym4	UTSW	4	126,801,005 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GAGCTAAAAGAGATTGAGTACTTCC -3'
(R):5'- AAATGCTTCCTTCAGTTCCAGC -3'

Sequencing Primer
(F):5'- GATTGAGTACTTCCCAGAAAAATGG -3'
(R):5'- GCCACAGCTGTTCGAGTTTC -3'

Posted On

2018-08-01