Mutagenetix > Incidental Mutation

Incidental Mutation 'R6784:Or10ag2'

531544

Institutional Source

Beutler Lab

Gene Symbol

Or10ag2

Ensembl Gene

ENSMUSG00000043274

Gene Name

olfactory receptor family 10 subfamily AG member 2

Synonyms

Olfr1123, MOR264-17, GA_x6K02T2Q125-48917235-48918206

MMRRC Submission

044898-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.281) question?

Stock #

R6784 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87248394-87249365 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 87248796 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 135 (R135C)

Ref Sequence

ENSEMBL: ENSMUSP00000058786 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054974] [ENSMUST00000216208]

AlphaFold

A2AT85

Predicted Effect

probably benign
Transcript: ENSMUST00000054974
AA Change: R135C

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000058786
Gene: ENSMUSG00000043274
AA Change: R135C

Domain	Start	End	E-Value	Type
Pfam:7tm_4	44	321	4.4e-52	PFAM
Pfam:7tm_1	54	303	2.6e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216208
AA Change: R133C

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.2%

Validation Efficiency

98% (46/47)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aass	C	T	6: 23,093,895 (GRCm39)	S142N	probably null	Het
Aida	A	G	1: 183,103,346 (GRCm39)	Y275C	probably damaging	Het
Arrdc4	A	G	7: 68,398,594 (GRCm39)	I74T	probably benign	Het
C1ql4	A	G	15: 98,985,290 (GRCm39)	V107A	probably benign	Het
Ccnh	T	C	13: 85,360,884 (GRCm39)	V284A	probably benign	Het
Cd180	A	G	13: 102,839,213 (GRCm39)	E32G	probably damaging	Het
Chd6	T	C	2: 160,808,174 (GRCm39)	D1680G	probably damaging	Het
Cog7	A	T	7: 121,563,516 (GRCm39)		probably null	Het
Cyp2j6	T	A	4: 96,423,741 (GRCm39)	Q209L	possibly damaging	Het
Dars1	A	T	1: 128,319,084 (GRCm39)	V116E	probably damaging	Het
Dnah10	A	G	5: 124,854,890 (GRCm39)	K1932E	probably damaging	Het
Dock9	A	G	14: 121,780,926 (GRCm39)	S2086P	probably damaging	Het
Espl1	C	G	15: 102,207,660 (GRCm39)	R375G	probably benign	Het
Fer1l6	C	T	15: 58,443,275 (GRCm39)	S526L	possibly damaging	Het
Galnt9	G	A	5: 110,768,465 (GRCm39)	R587H	probably damaging	Het
Gigyf2	G	T	1: 87,371,396 (GRCm39)	V1170L	probably damaging	Het
Gm14412	G	T	2: 177,009,133 (GRCm39)	T41K	probably benign	Het
Gm45861	A	G	8: 27,990,086 (GRCm39)		probably null	Het
H2bc4	T	C	13: 23,868,483 (GRCm39)	I90T	probably damaging	Het
Hoxc5	A	G	15: 102,922,322 (GRCm39)		probably benign	Het
Jkampl	A	T	6: 73,445,918 (GRCm39)	Y210*	probably null	Het
Klra1	T	C	6: 130,349,817 (GRCm39)	D207G	probably benign	Het
Lancl2	A	G	6: 57,680,240 (GRCm39)	N57D	probably benign	Het
Map2k4	T	C	11: 65,582,577 (GRCm39)		probably benign	Het
Mgat3	G	T	15: 80,096,401 (GRCm39)	Q409H	probably damaging	Het
Mpp3	T	A	11: 101,892,974 (GRCm39)		probably null	Het
Myh1	T	A	11: 67,105,396 (GRCm39)	L1062Q	probably damaging	Het
Nebl	T	A	2: 17,439,725 (GRCm39)	K183*	probably null	Het
Npat	G	C	9: 53,469,458 (GRCm39)	D315H	probably damaging	Het
Nt5c2	A	G	19: 46,912,766 (GRCm39)	V63A	probably damaging	Het
Opn1sw	T	A	6: 29,379,846 (GRCm39)	E129D	probably damaging	Het
Or1e29	T	G	11: 73,667,676 (GRCm39)	H159P	probably damaging	Het
Or51t4	A	T	7: 102,597,722 (GRCm39)	T17S	possibly damaging	Het
Or5p1	A	T	7: 107,916,989 (GRCm39)	D296V	probably damaging	Het
Prdm16	T	C	4: 154,407,764 (GRCm39)	Y1153C	probably damaging	Het
Prdm6	T	A	18: 53,669,698 (GRCm39)	D105E	probably benign	Het
Pwwp4c	C	T	X: 73,684,021 (GRCm39)	R355H	probably damaging	Homo
Rbm27	T	C	18: 42,434,929 (GRCm39)	M331T	probably benign	Het
S1pr1	A	G	3: 115,505,710 (GRCm39)	Y295H	probably damaging	Het
Sgpp1	G	C	12: 75,782,243 (GRCm39)	P32R	probably benign	Het
Sla2	C	T	2: 156,725,589 (GRCm39)	S3N	unknown	Het
Slc10a6	A	G	5: 103,776,896 (GRCm39)	I68T	probably damaging	Het
Slc5a1	T	C	5: 33,315,460 (GRCm39)	F493S	probably benign	Het
Snx14	A	T	9: 88,263,845 (GRCm39)	Y847N	probably benign	Het
Tmc1	A	G	19: 20,805,015 (GRCm39)		probably null	Het
Vps8	C	A	16: 21,381,957 (GRCm39)	Q1130K	probably benign	Het

Other mutations in Or10ag2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Or10ag2	APN	2	87,248,910 (GRCm39)	missense	possibly damaging	0.75
IGL01067:Or10ag2	APN	2	87,248,714 (GRCm39)	missense	probably benign	0.00
IGL01526:Or10ag2	APN	2	87,249,319 (GRCm39)	missense	probably damaging	0.99
IGL01580:Or10ag2	APN	2	87,248,880 (GRCm39)	missense	probably benign	0.06
IGL01986:Or10ag2	APN	2	87,248,880 (GRCm39)	missense	probably benign	0.00
IGL02503:Or10ag2	APN	2	87,248,636 (GRCm39)	missense	probably benign	0.03
IGL02527:Or10ag2	APN	2	87,249,181 (GRCm39)	missense	probably damaging	1.00
IGL02731:Or10ag2	APN	2	87,249,051 (GRCm39)	missense	probably benign	0.00
IGL03215:Or10ag2	APN	2	87,248,412 (GRCm39)	missense	probably benign	0.05
IGL03366:Or10ag2	APN	2	87,248,587 (GRCm39)	missense	possibly damaging	0.88
R0645:Or10ag2	UTSW	2	87,248,612 (GRCm39)	nonsense	probably null
R1857:Or10ag2	UTSW	2	87,248,992 (GRCm39)	missense	probably damaging	1.00
R2175:Or10ag2	UTSW	2	87,248,500 (GRCm39)	missense	probably damaging	1.00
R3691:Or10ag2	UTSW	2	87,248,514 (GRCm39)	missense	probably benign	0.20
R4082:Or10ag2	UTSW	2	87,248,801 (GRCm39)	nonsense	probably null
R4635:Or10ag2	UTSW	2	87,249,043 (GRCm39)	missense	probably benign	0.05
R4877:Or10ag2	UTSW	2	87,248,907 (GRCm39)	nonsense	probably null
R5190:Or10ag2	UTSW	2	87,249,187 (GRCm39)	missense	probably damaging	1.00
R5253:Or10ag2	UTSW	2	87,249,012 (GRCm39)	missense	possibly damaging	0.64
R6266:Or10ag2	UTSW	2	87,249,350 (GRCm39)	missense	probably benign	0.01
R6909:Or10ag2	UTSW	2	87,248,959 (GRCm39)	missense	probably damaging	1.00
R7255:Or10ag2	UTSW	2	87,249,286 (GRCm39)	missense	probably damaging	0.96
R8076:Or10ag2	UTSW	2	87,248,889 (GRCm39)	missense	probably benign	0.00
R8116:Or10ag2	UTSW	2	87,249,081 (GRCm39)	missense	probably damaging	1.00
R8315:Or10ag2	UTSW	2	87,248,995 (GRCm39)	missense	probably damaging	1.00
R8888:Or10ag2	UTSW	2	87,248,659 (GRCm39)	missense	probably benign	0.00
R8895:Or10ag2	UTSW	2	87,248,659 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAAGAACTGACCCTGCTCTG -3'
(R):5'- CTGGTGGGATATCACAAATGAAGTG -3'

Sequencing Primer
(F):5'- TGCTCTGCAGACCCCTATG -3'
(R):5'- GAAGTGATTAATTCTGTTAGAGCCAC -3'

Posted On

2018-08-29