Mutagenetix > Incidental Mutation

Incidental Mutation 'R6744:Or10al3'

532808

Institutional Source

Beutler Lab

Gene Symbol

Or10al3

Ensembl Gene

ENSMUSG00000059964

Gene Name

olfactory receptor family 10 subfamily AL member 3

Synonyms

GA_x6K02T2PSCP-2159633-2160598, MOR263-7, Olfr119

MMRRC Submission

044861-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R6744 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38007576-38012611 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 38012336 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 258 (Y258*)

Ref Sequence

ENSEMBL: ENSMUSP00000150099 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080483] [ENSMUST00000213732]

AlphaFold

Q7TRJ5

Predicted Effect

probably null
Transcript: ENSMUST00000080483
AA Change: Y258*

SMART Domains

Protein: ENSMUSP00000092919
Gene: ENSMUSG00000059964
AA Change: Y258*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	314	5.6e-59	PFAM
Pfam:7TM_GPCR_Srsx	41	311	1.7e-5	PFAM
Pfam:7tm_1	47	296	3.1e-23	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213732
AA Change: Y258*

Meta Mutation Damage Score

0.9666

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 96.6%

Validation Efficiency

98% (47/48)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahi1	T	A	10: 20,841,466 (GRCm39)	L251H	probably damaging	Het
Alk	A	T	17: 72,910,077 (GRCm39)	S210T	probably benign	Het
Arap2	A	G	5: 62,906,281 (GRCm39)	F246S	probably damaging	Het
Atad5	T	A	11: 80,024,858 (GRCm39)	N1749K	probably benign	Het
C87436	T	A	6: 86,423,046 (GRCm39)	S207T	probably damaging	Het
C8b	C	T	4: 104,631,543 (GRCm39)	R53W	probably damaging	Het
Catsperg2	A	G	7: 29,409,244 (GRCm39)	V619A	probably benign	Het
Cdc73	G	A	1: 143,577,887 (GRCm39)		probably benign	Het
Cdh4	C	A	2: 179,489,180 (GRCm39)	H297Q	possibly damaging	Het
Col3a1	G	T	1: 45,377,782 (GRCm39)		probably benign	Het
Crybg2	A	G	4: 133,816,207 (GRCm39)	N1328S	probably damaging	Het
Ctnnb1	T	A	9: 120,782,025 (GRCm39)	V346E	probably damaging	Het
Ctns	C	T	11: 73,076,111 (GRCm39)	G308E	probably damaging	Het
Cxxc4	AGGCGGCGGCGGCGGCGGCGGCGGC	AGGCGGCGGCGGCGGCGGCGGCGGCGGC	3: 133,945,891 (GRCm39)		probably benign	Het
Dnah6	A	G	6: 73,014,532 (GRCm39)	I3685T	probably damaging	Het
Dock6	T	C	9: 21,742,770 (GRCm39)	H775R	probably damaging	Het
Eef1ece2	A	T	16: 20,449,116 (GRCm39)	K165N	probably damaging	Het
Fbxl3	A	T	14: 103,320,730 (GRCm39)	V239D	probably damaging	Het
Gh	T	A	11: 106,192,230 (GRCm39)	K55*	probably null	Het
Havcr2	T	G	11: 46,345,887 (GRCm39)		probably null	Het
Kcnq2	T	A	2: 180,727,099 (GRCm39)	H576L	possibly damaging	Het
Kifap3	A	G	1: 163,676,239 (GRCm39)	N398S	probably benign	Het
Lama5	G	A	2: 179,833,455 (GRCm39)	P1519L	probably damaging	Het
Mblac1	A	G	5: 138,192,682 (GRCm39)	E8G	possibly damaging	Het
Mtor	C	T	4: 148,543,112 (GRCm39)	T290I	probably benign	Het
Nceh1	T	C	3: 27,295,938 (GRCm39)	Y400H	probably damaging	Het
Nek9	A	G	12: 85,376,703 (GRCm39)	V226A	probably benign	Het
Or10a3	T	C	7: 108,480,037 (GRCm39)	T259A	probably damaging	Het
Or2d4	A	G	7: 106,543,741 (GRCm39)	S156P	probably damaging	Het
Otud4	C	A	8: 80,400,407 (GRCm39)	Y1039*	probably null	Het
Pax4	T	C	6: 28,442,396 (GRCm39)	H331R	probably benign	Het
Piezo2	A	T	18: 63,165,960 (GRCm39)	Y2090*	probably null	Het
Ppp1r12a	T	A	10: 108,066,395 (GRCm39)	H195Q	probably damaging	Het
Ppp6r2	A	G	15: 89,140,864 (GRCm39)		probably null	Het
Prodh	A	G	16: 17,897,064 (GRCm39)	V23A	probably benign	Het
Psg20	G	A	7: 18,408,505 (GRCm39)	T405I	probably damaging	Het
Ptprf	T	C	4: 118,093,562 (GRCm39)	D360G	probably benign	Het
Qrfprl	T	A	6: 65,418,324 (GRCm39)	M164K	possibly damaging	Het
Rad18	A	T	6: 112,652,745 (GRCm39)	M284K	probably damaging	Het
Rgs17	T	C	10: 5,792,567 (GRCm39)	K60E	possibly damaging	Het
Sec31a	G	T	5: 100,540,358 (GRCm39)	Q39K	possibly damaging	Het
Slc22a22	G	T	15: 57,117,668 (GRCm39)	T291K	possibly damaging	Het
Sult2a6	C	T	7: 13,956,470 (GRCm39)	E264K	probably damaging	Het
Syne2	C	T	12: 76,121,221 (GRCm39)	R5896C	probably damaging	Het
Tctn1	A	T	5: 122,402,209 (GRCm39)	V75D	probably damaging	Het
Tmem214	A	G	5: 31,031,372 (GRCm39)	K409E	probably damaging	Het
Vcan	T	C	13: 89,853,301 (GRCm39)	Y553C	probably damaging	Het
Vmn1r49	C	T	6: 90,049,184 (GRCm39)	V273I	probably benign	Het

Other mutations in Or10al3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02209:Or10al3	APN	17	38,011,883 (GRCm39)	missense	probably damaging	1.00
IGL03339:Or10al3	APN	17	38,011,682 (GRCm39)	missense	probably damaging	0.99
R0092:Or10al3	UTSW	17	38,011,696 (GRCm39)	missense	probably damaging	0.98
R0207:Or10al3	UTSW	17	38,011,949 (GRCm39)	nonsense	probably null
R0378:Or10al3	UTSW	17	38,011,932 (GRCm39)	missense	probably damaging	1.00
R0408:Or10al3	UTSW	17	38,012,190 (GRCm39)	missense	probably benign
R0483:Or10al3	UTSW	17	38,012,188 (GRCm39)	missense	probably benign	0.01
R1595:Or10al3	UTSW	17	38,012,004 (GRCm39)	missense	probably benign	0.03
R1901:Or10al3	UTSW	17	38,012,312 (GRCm39)	missense	probably damaging	1.00
R1902:Or10al3	UTSW	17	38,012,312 (GRCm39)	missense	probably damaging	1.00
R2845:Or10al3	UTSW	17	38,011,714 (GRCm39)	missense	probably damaging	1.00
R2846:Or10al3	UTSW	17	38,011,714 (GRCm39)	missense	probably damaging	1.00
R4356:Or10al3	UTSW	17	38,011,790 (GRCm39)	missense	probably damaging	0.97
R4381:Or10al3	UTSW	17	38,011,790 (GRCm39)	missense	probably damaging	0.97
R7674:Or10al3	UTSW	17	38,011,573 (GRCm39)	missense	probably benign	0.03
R7677:Or10al3	UTSW	17	38,011,957 (GRCm39)	missense	probably damaging	1.00
R7994:Or10al3	UTSW	17	38,012,326 (GRCm39)	missense	probably damaging	0.99
R8305:Or10al3	UTSW	17	38,012,389 (GRCm39)	missense	probably benign	0.10
R8512:Or10al3	UTSW	17	38,012,071 (GRCm39)	missense	probably damaging	1.00
R9300:Or10al3	UTSW	17	38,011,815 (GRCm39)	missense	probably damaging	1.00
R9760:Or10al3	UTSW	17	38,012,434 (GRCm39)	missense	probably damaging	1.00
Z1177:Or10al3	UTSW	17	38,011,944 (GRCm39)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- TGCCTGTGGAGATACATCCC -3'
(R):5'- CAGCTCTGCAAGGTTCTGTTAC -3'

Sequencing Primer
(F):5'- GCCTGTGGAGATACATCCCAAAATG -3'
(R):5'- ATTGCCAGAGGTTTCTTCAGAC -3'

Posted On

2018-08-29