Mutagenetix > Incidental Mutation

Incidental Mutation 'R6746:Tarm1'

532829

Institutional Source

Beutler Lab

Gene Symbol

Tarm1

Ensembl Gene

ENSMUSG00000053338

Gene Name

T cell-interacting, activating receptor on myeloid cells 1

Synonyms

Gm9904, 9930022N03Rik

MMRRC Submission

044863-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R6746 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

3535016-3551140 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 3550978 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 2 (I2F)

Ref Sequence

ENSEMBL: ENSMUSP00000069745 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065703] [ENSMUST00000203020] [ENSMUST00000203821]

AlphaFold

B6A8R8

Predicted Effect

probably benign
Transcript: ENSMUST00000065703
AA Change: I2F

PolyPhen 2 Score 0.100 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000069745
Gene: ENSMUSG00000053338
AA Change: I2F

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
IG_like	34	120	9.45e0	SMART
IG	131	217	5.28e-3	SMART
transmembrane domain	256	278	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000203020
AA Change: I2F

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000145188
Gene: ENSMUSG00000053338
AA Change: I2F

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
IG_like	40	104	4.6e-3	SMART
IG_like	92	198	4.1e-2	SMART
IG	131	217	2.2e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000203821
AA Change: I2F

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000145491
Gene: ENSMUSG00000053338
AA Change: I2F

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
IG_like	34	120	9.45e0	SMART
IG	131	217	5.28e-3	SMART
transmembrane domain	256	278	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.3%

Validation Efficiency

100% (58/58)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	T	17: 24,565,195 (GRCm39)	L79*	probably null	Het
Acot3	A	G	12: 84,100,248 (GRCm39)	N8S	probably benign	Het
Adora2a	T	C	10: 75,169,442 (GRCm39)	V302A	probably benign	Het
Anpep	A	C	7: 79,488,933 (GRCm39)		probably null	Het
Arrb1	A	G	7: 99,250,357 (GRCm39)	K392E	probably benign	Het
Atp8a1	A	T	5: 67,908,392 (GRCm39)	N444K	probably benign	Het
Bltp3b	T	A	10: 89,623,020 (GRCm39)	N298K	probably benign	Het
Brinp2	C	T	1: 158,094,160 (GRCm39)	G181R	probably benign	Het
Cacna1g	A	T	11: 94,300,253 (GRCm39)	C2184*	probably null	Het
Cacna1h	C	T	17: 25,600,524 (GRCm39)	A1606T	probably damaging	Het
Ccdc30	T	C	4: 119,213,915 (GRCm39)	T205A	probably benign	Het
Celsr1	A	G	15: 85,915,696 (GRCm39)	I759T	probably damaging	Het
Chaf1a	A	G	17: 56,370,404 (GRCm39)	D623G	possibly damaging	Het
Col6a3	G	T	1: 90,706,767 (GRCm39)	N2115K	unknown	Het
Dync1h1	A	T	12: 110,618,087 (GRCm39)	T3209S	probably damaging	Het
Erich6	T	C	3: 58,523,987 (GRCm39)	D629G	possibly damaging	Het
Fahd1	G	T	17: 25,068,915 (GRCm39)	A54E	probably damaging	Het
Flrt3	C	T	2: 140,501,945 (GRCm39)	R561Q	probably damaging	Het
Grm6	A	T	11: 50,747,790 (GRCm39)	D334V	probably damaging	Het
Helb	A	T	10: 119,941,373 (GRCm39)	D438E	probably damaging	Het
Hmgcs1	T	C	13: 120,156,585 (GRCm39)		probably null	Het
Hnf4g	C	A	3: 3,722,170 (GRCm39)	Y441*	probably null	Het
Hspa13	A	T	16: 75,561,925 (GRCm39)	N91K	possibly damaging	Het
Ilvbl	T	C	10: 78,413,057 (GRCm39)	I193T	possibly damaging	Het
Itga7	T	C	10: 128,785,341 (GRCm39)	V848A	probably benign	Het
Kash5	C	T	7: 44,849,735 (GRCm39)	V63I	probably benign	Het
Lpin1	A	G	12: 16,615,529 (GRCm39)	M341T	probably benign	Het
Lypd5	G	T	7: 24,052,531 (GRCm39)		probably null	Het
Mrgprb1	A	C	7: 48,097,645 (GRCm39)	V89G	possibly damaging	Het
Nsun7	T	C	5: 66,441,080 (GRCm39)		probably null	Het
Oasl1	T	A	5: 115,075,242 (GRCm39)	V434E	probably damaging	Het
Or2ak6	G	A	11: 58,593,369 (GRCm39)	V281I	probably benign	Het
Or7g22	A	G	9: 19,048,774 (GRCm39)	M162V	probably benign	Het
Or8k16	T	C	2: 85,519,952 (GRCm39)	Y60H	probably damaging	Het
Otor	T	A	2: 142,921,955 (GRCm39)		probably null	Het
Pik3cg	G	A	12: 32,244,757 (GRCm39)	T899M	probably damaging	Het
Plaat5	A	G	19: 7,590,695 (GRCm39)	D74G	probably benign	Het
Pld2	C	A	11: 70,431,933 (GRCm39)	L52M	probably damaging	Het
Pon3	A	T	6: 5,230,786 (GRCm39)	M247K	possibly damaging	Het
Ppfia2	C	A	10: 106,742,319 (GRCm39)	Y1037*	probably null	Het
Ppm1m	A	T	9: 106,075,351 (GRCm39)	C99*	probably null	Het
Prss44	A	T	9: 110,644,361 (GRCm39)	*145C	probably null	Het
Ptpro	T	A	6: 137,371,821 (GRCm39)	Y613N	probably damaging	Het
Ralgapb	T	G	2: 158,318,056 (GRCm39)	V866G	probably damaging	Het
Rassf6	C	A	5: 90,757,633 (GRCm39)	R109L	possibly damaging	Het
Rbm4b	A	C	19: 4,812,031 (GRCm39)	T147P	probably benign	Het
Rpl7l1	T	C	17: 47,090,322 (GRCm39)	K104R	probably benign	Het
Ryr1	A	T	7: 28,816,829 (GRCm39)	I69N	possibly damaging	Het
Scd1	G	T	19: 44,394,927 (GRCm39)	F99L	probably benign	Het
Semp2l1	A	T	1: 32,585,844 (GRCm39)	I22N	probably benign	Het
Spint2	A	T	7: 28,958,848 (GRCm39)	S66T	probably benign	Het
Tenm4	T	C	7: 96,542,067 (GRCm39)	V1860A	probably damaging	Het
Usp38	A	G	8: 81,740,920 (GRCm39)	I49T	possibly damaging	Het
Vars2	A	G	17: 35,971,294 (GRCm39)		probably null	Het
Vmn2r27	T	G	6: 124,177,552 (GRCm39)	H484P	possibly damaging	Het
Wdr35	T	A	12: 9,053,982 (GRCm39)		probably null	Het
Zfp937	A	T	2: 150,081,343 (GRCm39)	K458*	probably null	Het

Other mutations in Tarm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03229:Tarm1	APN	7	3,545,413 (GRCm39)	missense	probably damaging	1.00
R0096:Tarm1	UTSW	7	3,546,067 (GRCm39)	missense	probably benign	0.23
R0282:Tarm1	UTSW	7	3,546,006 (GRCm39)	missense	probably damaging	1.00
R0308:Tarm1	UTSW	7	3,545,187 (GRCm39)	splice site	probably benign
R3768:Tarm1	UTSW	7	3,546,097 (GRCm39)	missense	probably benign	0.10
R4732:Tarm1	UTSW	7	3,545,416 (GRCm39)	nonsense	probably null
R4733:Tarm1	UTSW	7	3,545,416 (GRCm39)	nonsense	probably null
R4982:Tarm1	UTSW	7	3,537,612 (GRCm39)	missense	probably damaging	1.00
R5336:Tarm1	UTSW	7	3,546,084 (GRCm39)	missense	probably damaging	0.99
R6128:Tarm1	UTSW	7	3,537,720 (GRCm39)	missense	probably benign	0.04
R6892:Tarm1	UTSW	7	3,546,006 (GRCm39)	missense	probably damaging	1.00
R7003:Tarm1	UTSW	7	3,545,939 (GRCm39)	critical splice donor site	probably null
R7414:Tarm1	UTSW	7	3,545,318 (GRCm39)	missense	probably benign	0.05
R8439:Tarm1	UTSW	7	3,546,037 (GRCm39)	missense	possibly damaging	0.69
R8925:Tarm1	UTSW	7	3,537,719 (GRCm39)	missense	possibly damaging	0.94
R8927:Tarm1	UTSW	7	3,537,719 (GRCm39)	missense	possibly damaging	0.94
R9608:Tarm1	UTSW	7	3,551,062 (GRCm39)	start gained	probably benign
R9687:Tarm1	UTSW	7	3,544,457 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GCCCTAACATGTACAGTGAACTC -3'
(R):5'- TGAGGCAAGTCTCTGAAGGG -3'

Sequencing Primer
(F):5'- AACTCACTGAAATTTCTGGTTTGTCC -3'
(R):5'- TGTTGCAACAGATAGCATCAGC -3'

Posted On

2018-08-29