Mutagenetix > Incidental Mutation

Incidental Mutation 'R6809:Or5g25'

533690

Institutional Source

Beutler Lab

Gene Symbol

Or5g25

Ensembl Gene

ENSMUSG00000075214

Gene Name

olfactory receptor family 5 subfamily G member 25

Synonyms

MOR175-2, Olfr1002, GA_x6K02T2Q125-47127746-47126790

MMRRC Submission

044922-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R6809 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85477707-85478663 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 85478317 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 116 (A116V)

Ref Sequence

ENSEMBL: ENSMUSP00000150405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099920] [ENSMUST00000215548]

AlphaFold

Q8VFK2

Predicted Effect

probably damaging
Transcript: ENSMUST00000099920
AA Change: A116V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097504
Gene: ENSMUSG00000075214
AA Change: A116V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.7e-50	PFAM
Pfam:7tm_1	41	290	2.5e-17	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215548
AA Change: A116V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.1%

Validation Efficiency

96% (51/53)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A130010J15Rik	T	A	1: 192,857,089 (GRCm39)	V147E	possibly damaging	Het
Abtb3	A	G	10: 85,467,240 (GRCm39)	T732A	probably benign	Het
Adgrv1	A	T	13: 81,621,072 (GRCm39)	M3893K	probably benign	Het
Aox3	T	C	1: 58,157,840 (GRCm39)	S66P	probably damaging	Het
Apeh	G	A	9: 107,969,878 (GRCm39)	H186Y	probably damaging	Het
Bloc1s5	A	G	13: 38,787,961 (GRCm39)	V141A	probably benign	Het
Bltp1	A	G	3: 36,928,431 (GRCm39)	N61S	probably damaging	Het
Col28a1	T	C	6: 7,999,468 (GRCm39)	Y1083C	probably damaging	Het
Crb1	A	G	1: 139,170,864 (GRCm39)	V842A	probably benign	Het
Dapk1	A	G	13: 60,899,103 (GRCm39)	S858G	probably benign	Het
Dlgap2	G	T	8: 14,229,619 (GRCm39)		probably benign	Het
Dpysl4	A	G	7: 138,673,576 (GRCm39)	D199G	probably benign	Het
Eml6	C	T	11: 29,753,161 (GRCm39)	V917I	probably benign	Het
Fut9	T	A	4: 25,620,647 (GRCm39)	T56S	probably benign	Het
Gtdc1	G	C	2: 44,465,563 (GRCm39)	S214*	probably null	Het
Gtdc1	A	G	2: 44,715,396 (GRCm39)	F49S	probably damaging	Het
Hoxd9	A	G	2: 74,529,590 (GRCm39)	Y282C	probably damaging	Het
Hrc	A	G	7: 44,985,803 (GRCm39)	E318G	probably benign	Het
Ikzf2	T	C	1: 69,609,661 (GRCm39)	T195A	probably damaging	Het
Krt36	A	T	11: 99,996,335 (GRCm39)	S30T	probably benign	Het
Lrp1	A	G	10: 127,390,925 (GRCm39)	F2991L	probably benign	Het
Lrrc34	T	A	3: 30,688,749 (GRCm39)	Q184L	possibly damaging	Het
Mapk1	A	T	16: 16,853,326 (GRCm39)	M49L	probably benign	Het
Mroh2a	G	T	1: 88,162,938 (GRCm39)	Q360H	probably benign	Het
Mrpl48	A	G	7: 100,195,574 (GRCm39)		probably benign	Het
Ncln	A	G	10: 81,323,512 (GRCm39)		probably null	Het
Nol4	T	A	18: 23,053,882 (GRCm39)	Q96L	probably damaging	Het
Or2y1e	G	T	11: 49,218,687 (GRCm39)	V150L	probably benign	Het
Padi2	A	G	4: 140,674,077 (GRCm39)		probably null	Het
Pak5	T	G	2: 135,939,501 (GRCm39)	I545L	possibly damaging	Het
Paqr5	T	A	9: 61,876,064 (GRCm39)	R157W	probably null	Het
Pard3b	G	A	1: 62,200,340 (GRCm39)	R293H	probably damaging	Het
Phf11d	C	A	14: 59,593,844 (GRCm39)	C110F	probably damaging	Het
Pkn2	G	A	3: 142,504,765 (GRCm39)	P804S	probably damaging	Het
Pnpla6	G	A	8: 3,584,611 (GRCm39)	C743Y	possibly damaging	Het
Pramel14	A	G	4: 143,719,651 (GRCm39)	V238A	probably benign	Het
Pramel58	C	A	5: 94,831,277 (GRCm39)	Q95K	probably benign	Het
Psip1	A	G	4: 83,386,879 (GRCm39)	M164T	probably benign	Het
Rgs22	C	G	15: 36,048,910 (GRCm39)	S914T	probably damaging	Het
Rufy4	G	A	1: 74,172,206 (GRCm39)	V310I	probably benign	Het
Ryr2	G	A	13: 11,741,816 (GRCm39)	L2122F	probably damaging	Het
Sgcb	T	G	5: 73,798,036 (GRCm39)	N142T	probably benign	Het
Slc27a6	A	G	18: 58,738,126 (GRCm39)	E394G	probably benign	Het
Slc29a2	T	C	19: 5,079,271 (GRCm39)	V298A	probably damaging	Het
Slf2	C	A	19: 44,931,907 (GRCm39)	T654N	probably damaging	Het
Spata4	A	C	8: 55,055,368 (GRCm39)	K123Q	possibly damaging	Het
Taar7b	T	C	10: 23,876,756 (GRCm39)	V307A	probably benign	Het
Tenm4	G	A	7: 96,202,703 (GRCm39)	R106H	probably benign	Het
Ttn	T	C	2: 76,693,983 (GRCm39)	E295G	possibly damaging	Het
Ubqln4	A	T	3: 88,462,679 (GRCm39)	Q48L	possibly damaging	Het
Ugt1a10	T	A	1: 87,983,647 (GRCm39)	D148E	probably damaging	Het
Vmn2r85	T	A	10: 130,261,795 (GRCm39)	N181Y	probably benign	Het
Wdfy3	C	T	5: 102,071,813 (GRCm39)	V1026I	possibly damaging	Het

Other mutations in Or5g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02591:Or5g25	APN	2	85,478,487 (GRCm39)	missense	probably damaging	0.98
IGL02873:Or5g25	APN	2	85,478,096 (GRCm39)	missense	possibly damaging	0.50
PIT4362001:Or5g25	UTSW	2	85,478,068 (GRCm39)	missense	probably damaging	1.00
R1241:Or5g25	UTSW	2	85,477,904 (GRCm39)	missense	probably damaging	1.00
R1261:Or5g25	UTSW	2	85,478,143 (GRCm39)	missense	probably damaging	1.00
R1666:Or5g25	UTSW	2	85,478,157 (GRCm39)	nonsense	probably null
R1902:Or5g25	UTSW	2	85,478,201 (GRCm39)	missense	possibly damaging	0.81
R1965:Or5g25	UTSW	2	85,478,090 (GRCm39)	missense	possibly damaging	0.94
R2096:Or5g25	UTSW	2	85,478,434 (GRCm39)	missense	probably benign	0.20
R4239:Or5g25	UTSW	2	85,478,647 (GRCm39)	missense	probably damaging	0.98
R4730:Or5g25	UTSW	2	85,478,336 (GRCm39)	missense	probably benign	0.39
R4948:Or5g25	UTSW	2	85,477,916 (GRCm39)	missense	probably benign	0.30
R5627:Or5g25	UTSW	2	85,477,991 (GRCm39)	missense	probably damaging	1.00
R5844:Or5g25	UTSW	2	85,478,239 (GRCm39)	missense	probably benign	0.36
R7399:Or5g25	UTSW	2	85,477,768 (GRCm39)	missense	possibly damaging	0.89
R7476:Or5g25	UTSW	2	85,478,512 (GRCm39)	missense	not run
R7805:Or5g25	UTSW	2	85,477,794 (GRCm39)	nonsense	probably null
R7960:Or5g25	UTSW	2	85,478,417 (GRCm39)	missense	possibly damaging	0.82
R8015:Or5g25	UTSW	2	85,478,136 (GRCm39)	missense	probably damaging	0.99
R8355:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8455:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8479:Or5g25	UTSW	2	85,478,447 (GRCm39)	missense	probably damaging	1.00
R8683:Or5g25	UTSW	2	85,478,410 (GRCm39)	missense	probably benign	0.35
R8699:Or5g25	UTSW	2	85,478,330 (GRCm39)	missense	possibly damaging	0.87
R8762:Or5g25	UTSW	2	85,478,034 (GRCm39)	missense	probably damaging	1.00
R8897:Or5g25	UTSW	2	85,478,187 (GRCm39)	missense	possibly damaging	0.92
R9280:Or5g25	UTSW	2	85,478,504 (GRCm39)	nonsense	probably null
R9674:Or5g25	UTSW	2	85,478,593 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- TGATTGATGAGATTTGGACCACAG -3'
(R):5'- TCGCAACTCTTCTGGGGAAC -3'

Sequencing Primer
(F):5'- ACAGTATGGAAGAATAAATGCAGAAG -3'
(R):5'- GGGAATGATCATTCTTATTCACCAGG -3'

Posted On

2018-09-12