Mutagenetix > Incidental Mutation

Incidental Mutation 'R6813:Or10g9b'

533957

Institutional Source

Beutler Lab

Gene Symbol

Or10g9b

Ensembl Gene

ENSMUSG00000060254

Gene Name

olfactory receptor family 10 subfamily G member 9B

Synonyms

MOR223-2, GA_x6K02T2PVTD-33705428-33704496, Olfr980

MMRRC Submission

044925-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6813 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

39917290-39918325 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 39917753 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 164 (H164L)

Ref Sequence

ENSEMBL: ENSMUSP00000150496 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073932] [ENSMUST00000215523] [ENSMUST00000216463]

AlphaFold

Q8VH08

Predicted Effect

probably benign
Transcript: ENSMUST00000073932
AA Change: H164L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000091386
Gene: ENSMUSG00000060254
AA Change: H164L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	1.4e-55	PFAM
Pfam:7tm_1	39	287	5.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215523

Predicted Effect

probably benign
Transcript: ENSMUST00000216463
AA Change: H164L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Meta Mutation Damage Score

0.2575

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 98.9%
20x: 96.9%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb2	C	A	4: 129,903,284 (GRCm39)	Q603K	probably damaging	Het
Adgrf3	A	G	5: 30,402,519 (GRCm39)	F503S	probably damaging	Het
Arfgap3	A	T	15: 83,214,794 (GRCm39)	M164K	probably benign	Het
Asb13	G	T	13: 3,695,029 (GRCm39)	V166F	probably damaging	Het
Atm	T	G	9: 53,408,535 (GRCm39)	R1103S	probably benign	Het
Atxn7l1	T	C	12: 33,417,123 (GRCm39)	I626T	probably damaging	Het
Brsk2	A	G	7: 141,556,214 (GRCm39)	I649V	probably benign	Het
Ccdc25	T	A	14: 66,093,882 (GRCm39)	M85K	probably benign	Het
Cdc42bpb	A	G	12: 111,294,049 (GRCm39)	V231A	probably damaging	Het
Clstn3	T	A	6: 124,413,894 (GRCm39)	M767L	probably benign	Het
Col6a6	C	T	9: 105,661,140 (GRCm39)	R323K	probably benign	Het
Cplane1	T	A	15: 8,258,766 (GRCm39)	N2337K	probably benign	Het
Creld1	A	G	6: 113,466,530 (GRCm39)	Y199C	probably damaging	Het
Csf1r	T	A	18: 61,245,806 (GRCm39)	D254E	probably benign	Het
Dab2ip	C	T	2: 35,620,485 (GRCm39)	Q1118*	probably null	Het
Dcun1d4	C	A	5: 73,678,300 (GRCm39)	S98R	possibly damaging	Het
Disp3	G	A	4: 148,344,387 (GRCm39)	P505L	probably benign	Het
Dlec1	A	T	9: 118,941,170 (GRCm39)	Q240L	probably benign	Het
Dnai4	T	C	4: 102,905,523 (GRCm39)	K753E	probably benign	Het
Edil3	T	A	13: 89,437,575 (GRCm39)	I392N	probably damaging	Het
Epha10	T	A	4: 124,796,486 (GRCm39)	S398R		Het
Ephb1	A	G	9: 101,887,247 (GRCm39)	I464T	possibly damaging	Het
Eps15	T	A	4: 109,137,599 (GRCm39)		probably null	Het
Fam111a	T	A	19: 12,564,706 (GRCm39)	C152S	probably damaging	Het
Flt3	T	C	5: 147,291,653 (GRCm39)	E599G	probably damaging	Het
Frmd3	T	C	4: 74,077,482 (GRCm39)	S259P	probably benign	Het
Hsfy2	A	G	1: 56,675,461 (GRCm39)	Y359H	possibly damaging	Het
Ifih1	C	T	2: 62,476,037 (GRCm39)	V80M	possibly damaging	Het
Il12rb2	A	C	6: 67,269,358 (GRCm39)	D818E	probably damaging	Het
Il4i1	A	G	7: 44,489,236 (GRCm39)	T334A	probably benign	Het
Irs2	G	A	8: 11,054,659 (GRCm39)	Q1258*	probably null	Het
Lsm1	T	G	8: 26,283,721 (GRCm39)	H44Q	probably benign	Het
Mgam	A	T	6: 40,727,099 (GRCm39)	M1257L	probably damaging	Het
Myc	T	C	15: 61,860,001 (GRCm39)	S225P	probably damaging	Het
Myh1	T	C	11: 67,111,286 (GRCm39)	V1575A	probably benign	Het
Myo9b	A	G	8: 71,775,949 (GRCm39)	D380G	probably damaging	Het
Ncoa7	T	A	10: 30,572,188 (GRCm39)	D157V	probably damaging	Het
Or2t44	G	T	11: 58,677,472 (GRCm39)	Q137H	probably benign	Het
Or7g16	G	A	9: 18,727,188 (GRCm39)	T134M	probably benign	Het
Or8b36	T	C	9: 37,937,129 (GRCm39)	V9A	probably damaging	Het
Or8b9	A	C	9: 37,766,810 (GRCm39)	E232A	possibly damaging	Het
Pccb	A	G	9: 100,905,268 (GRCm39)	V117A	probably damaging	Het
Pdp2	C	T	8: 105,321,131 (GRCm39)	H327Y	probably damaging	Het
Pdzph1	G	T	17: 59,281,431 (GRCm39)	Q284K	probably benign	Het
Phka2	G	A	X: 159,316,044 (GRCm39)	V230I	probably damaging	Het
Phldb1	A	T	9: 44,610,865 (GRCm39)	S751R	probably damaging	Het
Pira1	T	C	7: 3,739,002 (GRCm39)	H535R	probably benign	Het
Ppp1r1b	T	A	11: 98,240,002 (GRCm39)		probably null	Het
Ppp1r3a	A	G	6: 14,719,570 (GRCm39)	V448A	probably benign	Het
Pvrig-ps	A	T	5: 138,340,312 (GRCm39)	T28S	probably benign	Het
Rasgrf1	G	A	9: 89,892,537 (GRCm39)		probably null	Het
Scnn1g	T	C	7: 121,339,576 (GRCm39)	L125S	probably damaging	Het
Slc30a7	A	T	3: 115,775,460 (GRCm39)	D221E	probably benign	Het
Spata31e1	T	A	13: 49,940,872 (GRCm39)	R279S	probably benign	Het
Tmem30c	A	G	16: 57,101,622 (GRCm39)		probably null	Het
Tmem33	T	C	5: 67,421,802 (GRCm39)		probably null	Het
Ttc29	A	T	8: 79,060,249 (GRCm39)	T390S	probably benign	Het
Vamp5	G	A	6: 72,357,424 (GRCm39)		probably benign	Het
Vmn2r81	T	A	10: 79,104,439 (GRCm39)	F354Y	probably benign	Het
Vmn2r96	A	G	17: 18,802,116 (GRCm39)	H119R	probably benign	Het
Wdr3	G	A	3: 100,046,041 (GRCm39)	R931*	probably null	Het
Wtap	A	T	17: 13,186,397 (GRCm39)	N383K	probably damaging	Het
Zfp808	T	C	13: 62,320,849 (GRCm39)	Y693H	probably damaging	Het

Other mutations in Or10g9b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02256:Or10g9b	APN	9	39,917,349 (GRCm39)	missense	probably benign
IGL02378:Or10g9b	APN	9	39,917,769 (GRCm39)	missense	probably damaging	1.00
IGL03384:Or10g9b	APN	9	39,917,766 (GRCm39)	missense	probably benign
IGL03402:Or10g9b	APN	9	39,917,802 (GRCm39)	missense	probably benign	0.31
BB010:Or10g9b	UTSW	9	39,918,431 (GRCm39)	start gained	probably benign
PIT4651001:Or10g9b	UTSW	9	39,917,526 (GRCm39)	missense	probably damaging	0.97
R0013:Or10g9b	UTSW	9	39,917,651 (GRCm39)	missense	probably damaging	1.00
R1146:Or10g9b	UTSW	9	39,917,390 (GRCm39)	missense	possibly damaging	0.95
R1146:Or10g9b	UTSW	9	39,917,390 (GRCm39)	missense	possibly damaging	0.95
R4541:Or10g9b	UTSW	9	39,917,589 (GRCm39)	missense	possibly damaging	0.95
R4562:Or10g9b	UTSW	9	39,917,577 (GRCm39)	missense	probably damaging	0.99
R4731:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4732:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4733:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4825:Or10g9b	UTSW	9	39,918,038 (GRCm39)	missense	possibly damaging	0.72
R5619:Or10g9b	UTSW	9	39,918,039 (GRCm39)	missense	probably benign	0.07
R5770:Or10g9b	UTSW	9	39,917,634 (GRCm39)	missense	probably benign	0.01
R5791:Or10g9b	UTSW	9	39,918,030 (GRCm39)	missense	probably damaging	1.00
R6819:Or10g9b	UTSW	9	39,917,844 (GRCm39)	missense	probably benign	0.00
R6970:Or10g9b	UTSW	9	39,918,009 (GRCm39)	missense	probably benign	0.00
R7490:Or10g9b	UTSW	9	39,917,720 (GRCm39)	missense	probably damaging	1.00
R7511:Or10g9b	UTSW	9	39,918,229 (GRCm39)	missense	possibly damaging	0.63
R7933:Or10g9b	UTSW	9	39,918,431 (GRCm39)	start gained	probably benign
R8243:Or10g9b	UTSW	9	39,917,484 (GRCm39)	missense	probably benign	0.19
Z1088:Or10g9b	UTSW	9	39,917,892 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGAAGTGCGAATCCTCAGGATG -3'
(R):5'- TGGCACAGCTCTATTGCTTC -3'

Sequencing Primer
(F):5'- TCCTCAGGATGGAGCAGAC -3'
(R):5'- CACTTCCTGGGTAGCACCGAG -3'

Posted On

2018-09-12