Mutagenetix > Incidental Mutation

Incidental Mutation 'R6824:Vmn2r61'

534053

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

Gprc2a-rs2, Casr-rs2, EG637873

MMRRC Submission

044936-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R6824 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41909477-41950179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 41949403 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Leucine at position 608 (V608L)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably benign
Transcript: ENSMUST00000166131
AA Change: V608L

PolyPhen 2 Score 0.102 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: V608L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adck2	A	G	6: 39,552,058 (GRCm39)	D275G	probably benign	Het
Cdh4	G	A	2: 179,439,351 (GRCm39)	R166H	probably damaging	Het
Chl1	A	G	6: 103,691,510 (GRCm39)	K1051E	probably damaging	Het
Chn2	A	T	6: 54,249,938 (GRCm39)	M16L	probably benign	Het
Cmas	A	G	6: 142,716,962 (GRCm39)	T285A	possibly damaging	Het
Cnga4	A	T	7: 105,056,036 (GRCm39)	M213L	probably benign	Het
Ctif	C	T	18: 75,654,782 (GRCm39)	R248Q	probably damaging	Het
Cyth3	T	C	5: 143,672,265 (GRCm39)	I60T	probably damaging	Het
Dach1	A	G	14: 98,256,328 (GRCm39)	I310T	possibly damaging	Het
Defb6	T	C	8: 19,278,099 (GRCm39)	I57T	probably benign	Het
Dnajc27	T	C	12: 4,156,897 (GRCm39)	V262A	possibly damaging	Het
Emsy	G	A	7: 98,242,614 (GRCm39)	T1175M	probably benign	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Fam50b	G	A	13: 34,931,084 (GRCm39)	E187K	possibly damaging	Het
Fat4	T	A	3: 39,011,674 (GRCm39)	V2258E	probably benign	Het
Gm136	G	A	4: 34,746,591 (GRCm39)	T140I	probably benign	Het
Gps1	T	C	11: 120,678,254 (GRCm39)	F265S	probably damaging	Het
Grik5	C	T	7: 24,745,780 (GRCm39)	R431Q	possibly damaging	Het
Hnrnpul2	T	C	19: 8,804,081 (GRCm39)	V560A	possibly damaging	Het
Kmt2b	A	G	7: 30,285,701 (GRCm39)		probably benign	Het
Krt8	T	C	15: 101,906,875 (GRCm39)	N317S	possibly damaging	Het
Lad1	A	G	1: 135,755,479 (GRCm39)	T252A	probably benign	Het
Maml2	G	A	9: 13,608,513 (GRCm39)	S743N	possibly damaging	Het
Mrpl9	C	A	3: 94,350,677 (GRCm39)	P7H	possibly damaging	Het
Myb	T	A	10: 21,021,019 (GRCm39)	H470L	probably benign	Het
Nicol1	T	C	5: 34,141,069 (GRCm39)		probably benign	Het
Nr1i3	T	C	1: 171,042,542 (GRCm39)	I56T	probably benign	Het
Nrdc	A	G	4: 108,900,622 (GRCm39)	Y338C	probably damaging	Het
Or13n4	A	G	7: 106,423,664 (GRCm39)	V23A	probably benign	Het
Pcdhb20	T	A	18: 37,638,752 (GRCm39)	M426K	probably benign	Het
Pde6d	T	C	1: 86,473,485 (GRCm39)	T104A	possibly damaging	Het
Ptprz1	A	T	6: 23,002,130 (GRCm39)	T1407S	probably benign	Het
Recql5	C	T	11: 115,814,038 (GRCm39)	R369Q	possibly damaging	Het
Rnf180	T	A	13: 105,318,023 (GRCm39)	D463V	probably damaging	Het
Sart3	C	T	5: 113,882,600 (GRCm39)		probably null	Het
Sdk2	T	C	11: 113,758,760 (GRCm39)	D488G	probably benign	Het
Slc9a9	C	T	9: 95,109,251 (GRCm39)	P538S	probably damaging	Het
Snap29	A	G	16: 17,240,370 (GRCm39)	K159E	probably benign	Het
Spaca1	A	G	4: 34,049,869 (GRCm39)	V43A	probably benign	Het
Stk10	T	C	11: 32,537,363 (GRCm39)	S191P	probably damaging	Het
Tbc1d1	A	G	5: 64,414,245 (GRCm39)	H73R	probably benign	Het
Tcerg1l	A	G	7: 137,995,844 (GRCm39)		probably null	Het
Tmem67	T	C	4: 12,051,449 (GRCm39)	Y793C	probably damaging	Het
Ttll12	A	T	15: 83,475,578 (GRCm39)		probably null	Het
Xpo4	A	T	14: 57,850,860 (GRCm39)	I348N	probably damaging	Het
Zfp941	G	A	7: 140,392,612 (GRCm39)	T249M	probably benign	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	41,950,175 (GRCm39)	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	41,916,438 (GRCm39)	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	41,909,550 (GRCm39)	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	41,916,258 (GRCm39)	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	41,909,661 (GRCm39)	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	41,950,130 (GRCm39)	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	41,950,015 (GRCm39)	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	41,909,639 (GRCm39)	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	41,949,613 (GRCm39)	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	41,949,466 (GRCm39)	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	41,926,158 (GRCm39)	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	41,926,070 (GRCm39)	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	41,924,892 (GRCm39)	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	41,949,361 (GRCm39)	missense	probably benign
IGL03290:Vmn2r61	APN	7	41,915,408 (GRCm39)	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	41,916,509 (GRCm39)	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	41,909,517 (GRCm39)	missense	probably benign
IGL03402:Vmn2r61	APN	7	41,909,679 (GRCm39)	missense	probably benign
R0026:Vmn2r61	UTSW	7	41,924,898 (GRCm39)	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	41,949,941 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	41,915,335 (GRCm39)	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	41,915,442 (GRCm39)	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	41,949,844 (GRCm39)	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R1718:Vmn2r61	UTSW	7	41,950,121 (GRCm39)	missense	probably benign
R1835:Vmn2r61	UTSW	7	41,916,076 (GRCm39)	nonsense	probably null
R1920:Vmn2r61	UTSW	7	41,949,710 (GRCm39)	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	41,949,425 (GRCm39)	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	41,916,287 (GRCm39)	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	41,949,529 (GRCm39)	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	41,916,067 (GRCm39)	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	41,916,491 (GRCm39)	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	41,950,159 (GRCm39)	missense	probably benign
R4426:Vmn2r61	UTSW	7	41,950,157 (GRCm39)	missense	probably benign
R4484:Vmn2r61	UTSW	7	41,950,120 (GRCm39)	missense	probably benign
R4748:Vmn2r61	UTSW	7	41,916,565 (GRCm39)	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	41,916,459 (GRCm39)	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	41,950,132 (GRCm39)	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	41,916,520 (GRCm39)	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	41,949,478 (GRCm39)	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	41,949,953 (GRCm39)	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	41,909,646 (GRCm39)	missense	probably benign
R5497:Vmn2r61	UTSW	7	41,924,906 (GRCm39)	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	41,916,242 (GRCm39)	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	41,949,911 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,916,677 (GRCm39)	missense	probably benign	0.00
R5782:Vmn2r61	UTSW	7	41,949,253 (GRCm39)	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	41,916,455 (GRCm39)	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	41,909,616 (GRCm39)	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	41,915,915 (GRCm39)	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	41,949,242 (GRCm39)	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	41,916,659 (GRCm39)	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	41,915,283 (GRCm39)	nonsense	probably null
R6554:Vmn2r61	UTSW	7	41,926,139 (GRCm39)	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	41,949,580 (GRCm39)	missense	probably benign
R6768:Vmn2r61	UTSW	7	41,949,748 (GRCm39)	missense	probably damaging	1.00
R6930:Vmn2r61	UTSW	7	41,949,364 (GRCm39)	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	41,916,557 (GRCm39)	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	41,916,629 (GRCm39)	missense	possibly damaging	0.73
R7332:Vmn2r61	UTSW	7	41,909,534 (GRCm39)	missense	probably benign	0.00
R7359:Vmn2r61	UTSW	7	41,915,407 (GRCm39)	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	41,916,205 (GRCm39)	missense	not run
R7710:Vmn2r61	UTSW	7	41,916,472 (GRCm39)	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	41,916,097 (GRCm39)	missense	probably benign
R7839:Vmn2r61	UTSW	7	41,916,032 (GRCm39)	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	41,916,141 (GRCm39)	missense	probably benign	0.02
R8440:Vmn2r61	UTSW	7	41,916,080 (GRCm39)	missense	probably benign	0.02
R8499:Vmn2r61	UTSW	7	41,949,700 (GRCm39)	missense	probably damaging	0.99
R8771:Vmn2r61	UTSW	7	41,916,194 (GRCm39)	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	41,950,010 (GRCm39)	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	41,915,325 (GRCm39)	nonsense	probably null
R9290:Vmn2r61	UTSW	7	41,915,385 (GRCm39)	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	41,950,092 (GRCm39)	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	41,916,619 (GRCm39)	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	41,949,593 (GRCm39)	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	41,916,626 (GRCm39)	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	41,926,136 (GRCm39)	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	41,949,388 (GRCm39)	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	41,916,166 (GRCm39)	missense	probably benign	0.00
Z1176:Vmn2r61	UTSW	7	41,909,585 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GGATTGACACACGATCACTTTC -3'
(R):5'- TAGCTTTGGCCAACACTGTGG -3'

Sequencing Primer
(F):5'- ACACACGATCACTTTCATTTTTAAAC -3'
(R):5'- TTTGGCCAACACTGTGGAGAGAG -3'

Posted On

2018-09-12