Mutagenetix > Incidental Mutation

Incidental Mutation 'R6830:Mroh9'

534327

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

044940-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6830 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

162851871-162913239 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 162903935 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 26 (F26L)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

probably benign
Transcript: ENSMUST00000096608
AA Change: F26L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: F26L

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.1%

Validation Efficiency

98% (53/54)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503B20Rik	T	C	3: 146,356,716 (GRCm39)	D64G	possibly damaging	Het
Amtn	T	C	5: 88,525,956 (GRCm39)	L40P	probably damaging	Het
Ano6	A	G	15: 95,792,342 (GRCm39)	R90G	probably damaging	Het
Arfgef3	A	C	10: 18,540,637 (GRCm39)		probably null	Het
Asxl3	C	T	18: 22,658,445 (GRCm39)	P2152S	probably benign	Het
Atp7b	A	T	8: 22,512,381 (GRCm39)	V494E	probably damaging	Het
Bik	T	A	15: 83,428,409 (GRCm39)	Y146N	probably benign	Het
C1qtnf7	A	T	5: 43,766,436 (GRCm39)	I12F	possibly damaging	Het
Cacna1e	A	G	1: 154,289,720 (GRCm39)		probably null	Het
Ccdc168	A	G	1: 44,095,890 (GRCm39)	V1736A	probably benign	Het
Ccnj	A	G	19: 40,833,636 (GRCm39)	E231G	probably damaging	Het
Cd101	T	C	3: 100,901,012 (GRCm39)	K1020R	probably benign	Het
Cdh6	C	A	15: 13,044,860 (GRCm39)	V421L	probably benign	Het
Cplane1	T	C	15: 8,205,668 (GRCm39)	S135P	probably benign	Het
Decr1	T	C	4: 15,924,355 (GRCm39)		probably null	Het
Dmxl1	C	T	18: 50,054,091 (GRCm39)	P2566S	probably damaging	Het
Dock9	G	A	14: 121,860,330 (GRCm39)	P866L	probably damaging	Het
Epx	A	G	11: 87,759,452 (GRCm39)	F546L	probably damaging	Het
Fat4	T	A	3: 39,035,966 (GRCm39)	M3206K	probably benign	Het
Fgb	T	C	3: 82,952,332 (GRCm39)	D179G	probably benign	Het
Gbgt1	A	G	2: 28,395,220 (GRCm39)	D286G	probably damaging	Het
Gpn1	T	A	5: 31,664,832 (GRCm39)	S285R	probably benign	Het
Htt	T	A	5: 34,991,670 (GRCm39)	Y1212N	possibly damaging	Het
Kcnj13	T	C	1: 87,314,745 (GRCm39)	K159R	probably damaging	Het
Ldb2	T	A	5: 44,699,199 (GRCm39)	I80F	probably damaging	Het
Macrod2	T	A	2: 140,294,602 (GRCm39)	N89K	probably damaging	Het
Mdga2	G	A	12: 66,769,775 (GRCm39)	R173C	probably damaging	Het
Neil3	G	T	8: 54,052,514 (GRCm39)	N361K	probably benign	Het
Nepro	A	G	16: 44,551,720 (GRCm39)	R193G	probably damaging	Het
Or52d3	T	A	7: 104,229,447 (GRCm39)	I198N	probably damaging	Het
Pclo	A	T	5: 14,731,113 (GRCm39)	Q3205L	unknown	Het
Plekhm1	A	T	11: 103,267,715 (GRCm39)	I752N	probably damaging	Het
Podn	T	C	4: 107,878,614 (GRCm39)	T273A	possibly damaging	Het
Prep	T	A	10: 44,973,597 (GRCm39)	M235K	probably benign	Het
Ptprc	A	T	1: 137,999,993 (GRCm39)		probably null	Het
Reg2	A	G	6: 78,384,625 (GRCm39)	H119R	possibly damaging	Het
Rpe65	T	C	3: 159,319,805 (GRCm39)	V225A	probably benign	Het
Scn9a	T	C	2: 66,398,373 (GRCm39)	D79G	probably damaging	Het
Slc25a23	G	A	17: 57,360,804 (GRCm39)	R9*	probably null	Het
Snai3	A	G	8: 123,183,212 (GRCm39)	L111P	probably damaging	Het
Stk38	A	G	17: 29,218,981 (GRCm39)		probably null	Het
Stk38l	A	T	6: 146,668,269 (GRCm39)	I115F	possibly damaging	Het
Tmco6	T	A	18: 36,871,406 (GRCm39)		probably null	Het
Tollip	A	T	7: 141,452,451 (GRCm39)	M1K	probably null	Het
Trim40	T	C	17: 37,199,742 (GRCm39)	Y112C	possibly damaging	Het
Ttc27	T	A	17: 75,163,550 (GRCm39)	Y719*	probably null	Het
Ubald1	T	C	16: 4,697,584 (GRCm39)	D6G	probably damaging	Het
Vmn2r59	A	T	7: 41,693,171 (GRCm39)	S476R	probably benign	Het
Wfdc3	C	T	2: 164,576,178 (GRCm39)	G38R	possibly damaging	Het
Zbtb21	C	T	16: 97,753,161 (GRCm39)	G402D	probably damaging	Het
Zfp84	A	G	7: 29,475,911 (GRCm39)	Y201C	probably benign	Het
Zswim2	T	A	2: 83,770,028 (GRCm39)	H62L	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	162,873,350 (GRCm39)	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	162,907,072 (GRCm39)	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	162,852,227 (GRCm39)	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	162,888,191 (GRCm39)	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	162,866,700 (GRCm39)	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	162,875,435 (GRCm39)	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	162,908,144 (GRCm39)	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	162,883,172 (GRCm39)	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	162,875,551 (GRCm39)	splice site	probably null
IGL01823:Mroh9	APN	1	162,883,178 (GRCm39)	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	162,890,071 (GRCm39)	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	162,885,648 (GRCm39)	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	162,903,149 (GRCm39)	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	162,908,145 (GRCm39)	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	162,852,205 (GRCm39)	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	162,854,071 (GRCm39)	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	162,866,766 (GRCm39)	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	162,888,331 (GRCm39)	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0629:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	162,893,693 (GRCm39)	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	162,871,552 (GRCm39)	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	162,854,078 (GRCm39)	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	162,852,110 (GRCm39)	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	162,852,161 (GRCm39)	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	162,884,347 (GRCm39)	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	162,873,279 (GRCm39)	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	162,866,714 (GRCm39)	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	162,854,082 (GRCm39)	missense	probably damaging	0.97
R1923:Mroh9	UTSW	1	162,903,860 (GRCm39)	missense	probably damaging	1.00
R2325:Mroh9	UTSW	1	162,854,099 (GRCm39)	splice site	probably null
R2511:Mroh9	UTSW	1	162,866,514 (GRCm39)	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	162,884,341 (GRCm39)	missense	probably damaging	1.00
R2973:Mroh9	UTSW	1	162,884,338 (GRCm39)	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	162,893,638 (GRCm39)	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	162,908,122 (GRCm39)	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	162,871,662 (GRCm39)	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	162,893,593 (GRCm39)	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	162,883,162 (GRCm39)	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	162,852,061 (GRCm39)	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	162,854,093 (GRCm39)	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5157:Mroh9	UTSW	1	162,871,690 (GRCm39)	missense	probably damaging	0.96
R5324:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5325:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	162,908,156 (GRCm39)	start gained	probably benign
R6005:Mroh9	UTSW	1	162,903,246 (GRCm39)	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	162,893,612 (GRCm39)	nonsense	probably null
R6414:Mroh9	UTSW	1	162,902,271 (GRCm39)	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	162,903,873 (GRCm39)	missense	probably damaging	1.00
R6540:Mroh9	UTSW	1	162,866,541 (GRCm39)	missense	possibly damaging	0.87
R6541:Mroh9	UTSW	1	162,885,607 (GRCm39)	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	162,903,130 (GRCm39)	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	162,873,610 (GRCm39)	missense	possibly damaging	0.86
R7026:Mroh9	UTSW	1	162,888,251 (GRCm39)	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	162,866,525 (GRCm39)	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	162,866,750 (GRCm39)	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	162,903,858 (GRCm39)	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	162,902,277 (GRCm39)	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	162,873,601 (GRCm39)	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	162,852,122 (GRCm39)	missense	probably benign
R7808:Mroh9	UTSW	1	162,866,678 (GRCm39)	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	162,866,802 (GRCm39)	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	162,866,544 (GRCm39)	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	162,890,096 (GRCm39)	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	162,852,294 (GRCm39)	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	162,883,250 (GRCm39)	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	162,899,595 (GRCm39)	splice site	probably null
R8960:Mroh9	UTSW	1	162,883,196 (GRCm39)	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	162,890,069 (GRCm39)	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	162,875,412 (GRCm39)	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	162,890,030 (GRCm39)	missense
R9596:Mroh9	UTSW	1	162,893,576 (GRCm39)	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	162,866,498 (GRCm39)	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	162,885,630 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCTGAAATAGACTGAATGAGTTGTG -3'
(R):5'- AAGCACAATGGCTGGTTGG -3'

Sequencing Primer
(F):5'- GAGTTGTGAATAATTAACTCCTTGCG -3'
(R):5'- GGGGAATCATCACATGGT -3'

Posted On

2018-09-12