Incidental Mutation 'R6836:Alox8'
ID 534581
Institutional Source Beutler Lab
Gene Symbol Alox8
Ensembl Gene ENSMUSG00000020891
Gene Name arachidonate 8-lipoxygenase
Synonyms 8S-LOX, 8S-lipoxygenase, 8-LOX, Alox15b
Accession Numbers
Essential gene? Probably non essential (E-score: 0.114) question?
Stock # R6836 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 69074758-69088669 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 69080715 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Glycine at position 209 (R209G)
Ref Sequence ENSEMBL: ENSMUSP00000021262 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000021262] [ENSMUST00000094078]
AlphaFold O35936
Predicted Effect possibly damaging
Transcript: ENSMUST00000021262
AA Change: R209G

PolyPhen 2 Score 0.820 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000021262
Gene: ENSMUSG00000020891
AA Change: R209G

DomainStartEndE-ValueType
LH2 2 122 1.07e-36 SMART
Pfam:Lipoxygenase 163 662 5.5e-70 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000094078
AA Change: R209G

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
SMART Domains Protein: ENSMUSP00000091621
Gene: ENSMUSG00000020891
AA Change: R209G

DomainStartEndE-ValueType
LH2 2 122 1.07e-36 SMART
Pfam:Lipoxygenase 205 405 6.9e-44 PFAM
Pfam:Lipoxygenase 402 640 1.9e-60 PFAM
Meta Mutation Damage Score 0.0821 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 97.9%
Validation Efficiency 100% (62/62)
MGI Phenotype FUNCTION: This gene belongs to the lipoxygenase (LOX) gene family whose members encode enzymes that catalyze the addition of molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield fatty acid hydroperoxides. The encoded enzyme preferentially metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic acid (8-HETE), while metabolizing linoleic acid less efficiently. The gene may also function as a tumor suppressor. This gene is located in a cluster of related genes that spans approximately 75 kilobases on chromosome 11. [provided by RefSeq, Jan 2013]
Allele List at MGI
Other mutations in this stock
Total: 60 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamtsl2 T C 2: 26,971,718 (GRCm39) M1T probably null Het
Aim2 C G 1: 173,291,546 (GRCm39) T317R probably damaging Het
Asb5 A G 8: 55,038,106 (GRCm39) M210V probably benign Het
Atp2c2 T A 8: 120,461,154 (GRCm39) L249Q probably damaging Het
AU018091 T G 7: 3,213,986 (GRCm39) D77A probably damaging Het
Bahcc1 A T 11: 120,162,583 (GRCm39) K294* probably null Het
Baz2b C T 2: 59,747,769 (GRCm39) R1298Q probably damaging Het
Bivm T A 1: 44,182,296 (GRCm39) N501K possibly damaging Het
Bpifb5 T A 2: 154,069,985 (GRCm39) I145N probably benign Het
Casq2 A T 3: 101,994,076 (GRCm39) N41I probably damaging Het
Ccdc18 T C 5: 108,345,833 (GRCm39) L993P probably damaging Het
Cdcp3 A T 7: 130,798,233 (GRCm39) I81F possibly damaging Het
Cfap44 A G 16: 44,224,442 (GRCm39) D50G probably damaging Het
Clca3a2 T A 3: 144,512,144 (GRCm39) I91F probably damaging Het
Crebbp T A 16: 3,997,886 (GRCm39) H66L possibly damaging Het
Cyfip2 T C 11: 46,163,467 (GRCm39) T321A probably benign Het
Dido1 A G 2: 180,304,100 (GRCm39) V1268A probably benign Het
E030030I06Rik A C 10: 22,024,391 (GRCm39) V174G probably damaging Het
Gm20730 G T 6: 43,058,767 (GRCm39) probably null Het
Gm36176 T C 10: 77,682,976 (GRCm39) probably benign Het
Gper1 T A 5: 139,412,435 (GRCm39) M260K probably damaging Het
Igfbp2 T C 1: 72,888,817 (GRCm39) L89P probably damaging Het
Katnal1 T C 5: 148,830,974 (GRCm39) N200S probably damaging Het
Kcnk13 G T 12: 100,027,948 (GRCm39) R341L probably damaging Het
Klhl20 T A 1: 160,932,976 (GRCm39) E277D probably benign Het
Lingo1 T C 9: 56,527,056 (GRCm39) Y517C probably damaging Het
Lrrk1 C T 7: 65,992,527 (GRCm39) E82K probably benign Het
Mtor T A 4: 148,573,955 (GRCm39) V1275D possibly damaging Het
Ncan C T 8: 70,552,965 (GRCm39) S1089N possibly damaging Het
Notch4 C T 17: 34,805,074 (GRCm39) T1643I probably damaging Het
Or13a25 A C 7: 140,248,093 (GRCm39) I298L possibly damaging Het
Or1i2 A G 10: 78,448,424 (GRCm39) L17P probably damaging Het
Or2y15 A T 11: 49,350,904 (GRCm39) T133S possibly damaging Het
Or4c10 T C 2: 89,760,683 (GRCm39) C177R probably damaging Het
Pcdhgb6 T C 18: 37,876,015 (GRCm39) V241A probably benign Het
Phf11b T A 14: 59,565,572 (GRCm39) T102S possibly damaging Het
Pkd1 T A 17: 24,800,233 (GRCm39) V2998E probably damaging Het
Ppp1r13b T C 12: 111,801,629 (GRCm39) S352G probably benign Het
Ptprh C T 7: 4,554,134 (GRCm39) V778M probably damaging Het
Ptprz1 C T 6: 23,030,664 (GRCm39) Q1008* probably null Het
Ralgapa1 A T 12: 55,651,058 (GRCm39) probably null Het
Rbm20 G A 19: 53,802,500 (GRCm39) G336E probably damaging Het
Sdsl T C 5: 120,600,167 (GRCm39) I77V probably benign Het
Serpina3b G A 12: 104,100,341 (GRCm39) E308K probably benign Het
Sfrp5 G A 19: 42,190,149 (GRCm39) T101I probably damaging Het
Slc2a13 C T 15: 91,205,835 (GRCm39) V451I probably benign Het
Slc6a9 C T 4: 117,725,083 (GRCm39) A559V possibly damaging Het
Spg11 C T 2: 121,890,016 (GRCm39) A2109T probably damaging Het
Stard9 C T 2: 120,530,324 (GRCm39) R2194C probably benign Het
Tfeb T C 17: 48,097,123 (GRCm39) probably null Het
Tiam2 T A 17: 3,464,655 (GRCm39) I128N probably benign Het
Tmem161b C A 13: 84,370,537 (GRCm39) probably benign Het
Tpr T G 1: 150,312,424 (GRCm39) probably null Het
Traf3ip1 T C 1: 91,448,722 (GRCm39) I456T probably benign Het
Ttll4 T A 1: 74,728,572 (GRCm39) D892E probably damaging Het
Ubr1 T A 2: 120,727,156 (GRCm39) probably null Het
Vmn2r74 T A 7: 85,606,630 (GRCm39) I239F probably benign Het
Wdfy3 C T 5: 102,100,865 (GRCm39) V251M probably damaging Het
Xylb T A 9: 119,220,820 (GRCm39) L531H probably damaging Het
Zfp960 T A 17: 17,308,434 (GRCm39) C383S probably damaging Het
Other mutations in Alox8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01411:Alox8 APN 11 69,079,516 (GRCm39) missense probably benign
IGL01878:Alox8 APN 11 69,087,864 (GRCm39) missense probably benign 0.00
IGL02342:Alox8 APN 11 69,077,053 (GRCm39) missense probably damaging 1.00
IGL02694:Alox8 APN 11 69,077,455 (GRCm39) missense probably damaging 0.99
IGL03246:Alox8 APN 11 69,076,841 (GRCm39) missense probably damaging 1.00
IGL03373:Alox8 APN 11 69,077,443 (GRCm39) missense probably benign 0.00
R0567:Alox8 UTSW 11 69,082,348 (GRCm39) critical splice donor site probably null
R1575:Alox8 UTSW 11 69,076,067 (GRCm39) missense possibly damaging 0.94
R1688:Alox8 UTSW 11 69,080,732 (GRCm39) missense probably benign 0.01
R2021:Alox8 UTSW 11 69,077,114 (GRCm39) missense probably damaging 0.98
R2041:Alox8 UTSW 11 69,088,517 (GRCm39) missense possibly damaging 0.67
R2175:Alox8 UTSW 11 69,078,592 (GRCm39) missense possibly damaging 0.85
R2237:Alox8 UTSW 11 69,076,597 (GRCm39) missense probably benign 0.00
R3821:Alox8 UTSW 11 69,077,308 (GRCm39) missense probably damaging 0.98
R4870:Alox8 UTSW 11 69,077,394 (GRCm39) missense probably damaging 1.00
R6836:Alox8 UTSW 11 69,077,331 (GRCm39) missense probably damaging 1.00
R7003:Alox8 UTSW 11 69,082,416 (GRCm39) missense possibly damaging 0.70
R7158:Alox8 UTSW 11 69,076,696 (GRCm39) missense probably benign 0.00
R7316:Alox8 UTSW 11 69,077,064 (GRCm39) missense probably benign 0.01
R7513:Alox8 UTSW 11 69,078,670 (GRCm39) missense probably benign 0.34
R9515:Alox8 UTSW 11 69,075,950 (GRCm39) missense probably damaging 1.00
R9721:Alox8 UTSW 11 69,087,911 (GRCm39) missense probably benign 0.19
X0065:Alox8 UTSW 11 69,076,079 (GRCm39) missense probably damaging 1.00
Z1177:Alox8 UTSW 11 69,076,047 (GRCm39) missense probably damaging 1.00
Z1186:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1186:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1187:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1187:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1188:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1188:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1189:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1189:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1190:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1190:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1191:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1191:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1192:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1192:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- ACCTCTTTCGTGTCTATGGGAC -3'
(R):5'- ACTTACATGGTTCACTCAGCCC -3'

Sequencing Primer
(F):5'- GCATGGAGCCCAGCTAAAGTC -3'
(R):5'- TTCACTCAGCCCTGCCCAAG -3'
Posted On 2018-09-12