Mutagenetix > Incidental Mutation

Incidental Mutation 'R6851:Vmn2r96'

534988

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r96

Ensembl Gene

ENSMUSG00000091679

Gene Name

vomeronasal 2, receptor 96

Synonyms

EG433070

MMRRC Submission

044955-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.156) question?

Stock #

R6851 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

18793282-18818419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 18802800 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 237 (M237V)

Ref Sequence

ENSEMBL: ENSMUSP00000156012 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165692] [ENSMUST00000177244] [ENSMUST00000231261] [ENSMUST00000231286]

AlphaFold

E9PZU5

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165692
AA Change: M45V

PolyPhen 2 Score 0.852 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000131564
Gene: ENSMUSG00000091679
AA Change: M45V

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	257	8.6e-20	PFAM
Pfam:NCD3G	317	370	3.7e-22	PFAM
Pfam:7tm_3	402	638	3.9e-53	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000177244
AA Change: M237V

PolyPhen 2 Score 0.947 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000135095
Gene: ENSMUSG00000091679
AA Change: M237V

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	245	4.2e-19	PFAM
Pfam:NCD3G	317	370	6.9e-21	PFAM
Pfam:7tm_3	400	639	3.4e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231261

Predicted Effect

possibly damaging
Transcript: ENSMUST00000231286
AA Change: M237V

PolyPhen 2 Score 0.947 (Sensitivity: 0.79; Specificity: 0.95)

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (41/41)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	A	T	17: 46,623,345 (GRCm39)	C856S	probably benign	Het
Arrdc2	T	C	8: 71,291,369 (GRCm39)	E153G	probably damaging	Het
Cacna1d	T	C	14: 29,764,739 (GRCm39)	D2033G	probably damaging	Het
Cacna1s	G	A	1: 136,020,432 (GRCm39)	R823Q	probably benign	Het
Cracr2a	A	T	6: 127,585,679 (GRCm39)	D159V	probably damaging	Het
Defa35	T	C	8: 21,555,146 (GRCm39)	I22T	possibly damaging	Het
Dgke	T	C	11: 88,943,309 (GRCm39)	T227A	probably benign	Het
Efcab3	G	A	11: 104,896,521 (GRCm39)	R4290K	probably benign	Het
Firrm	G	A	1: 163,792,336 (GRCm39)	R554C	probably damaging	Het
Galnt10	G	A	11: 57,656,458 (GRCm39)	R214Q	probably damaging	Het
Gpr180	T	C	14: 118,391,037 (GRCm39)	Y189H	probably damaging	Het
Hadh	A	G	3: 131,065,620 (GRCm39)	S13P	possibly damaging	Het
Hcn2	T	A	10: 79,564,947 (GRCm39)		probably null	Het
Irgm2	T	C	11: 58,110,641 (GRCm39)	S123P	possibly damaging	Het
Kcnv1	T	A	15: 44,972,594 (GRCm39)	I430F	probably damaging	Het
Kif13b	G	A	14: 65,010,514 (GRCm39)	C1271Y	probably damaging	Het
Klhdc3	A	T	17: 46,989,218 (GRCm39)	I48N	possibly damaging	Het
Lama5	G	A	2: 179,833,455 (GRCm39)	P1519L	probably damaging	Het
Mprip	T	A	11: 59,649,841 (GRCm39)	W1182R	probably damaging	Het
Mycbp2	A	T	14: 103,497,630 (GRCm39)		probably null	Het
Or12e1	T	A	2: 87,022,813 (GRCm39)	S261T	probably benign	Het
Or1j11	T	A	2: 36,311,832 (GRCm39)	C141S	probably damaging	Het
Or8b51	A	G	9: 38,569,481 (GRCm39)	V69A	probably benign	Het
Or8h9	T	C	2: 86,789,611 (GRCm39)	T64A	possibly damaging	Het
Or9m1b	T	A	2: 87,836,300 (GRCm39)	H265L	probably damaging	Het
Osbpl8	T	A	10: 111,106,479 (GRCm39)	Y295*	probably null	Het
Pex5	A	G	6: 124,380,113 (GRCm39)	S275P	possibly damaging	Het
Prrc2c	TTGCTGCTGCTGCTGCTGCTGCTGCTGC	TTGCTGCTGCTGCTGCTGCTGCTGC	1: 162,536,630 (GRCm39)		probably benign	Het
Sele	G	T	1: 163,881,521 (GRCm39)	G543C	probably damaging	Het
Serpina3k	A	C	12: 104,311,625 (GRCm39)	Y401S	probably benign	Het
Slc13a4	A	G	6: 35,278,668 (GRCm39)	S74P	probably damaging	Het
Spata31d1a	A	G	13: 59,851,725 (GRCm39)	L114P	unknown	Het
Syne1	G	T	10: 5,212,703 (GRCm39)	C3295*	probably null	Het
Tepsin	G	T	11: 119,987,787 (GRCm39)	H44N	probably damaging	Het
Tpp1	A	T	7: 105,398,919 (GRCm39)	V170E	probably damaging	Het
Trav3-1	T	C	14: 52,818,428 (GRCm39)	V34A	probably damaging	Het
Tsbp1	A	T	17: 34,679,146 (GRCm39)	Y303F	possibly damaging	Het
Ush2a	T	C	1: 188,265,402 (GRCm39)	V1642A	probably benign	Het
Vmn1r122	A	G	7: 20,867,845 (GRCm39)	I70T	probably benign	Het
Wdr75	A	G	1: 45,862,587 (GRCm39)	E802G	probably benign	Het
Zan	G	A	5: 137,394,453 (GRCm39)	T4462I	unknown	Het

Other mutations in Vmn2r96

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Vmn2r96	APN	17	18,804,077 (GRCm39)	missense	probably benign	0.00
IGL00563:Vmn2r96	APN	17	18,804,077 (GRCm39)	missense	probably benign	0.00
IGL00769:Vmn2r96	APN	17	18,804,081 (GRCm39)	missense	probably benign	0.00
IGL01314:Vmn2r96	APN	17	18,803,226 (GRCm39)	missense	probably benign	0.00
IGL01983:Vmn2r96	APN	17	18,817,527 (GRCm39)	missense	probably damaging	0.99
IGL02314:Vmn2r96	APN	17	18,804,221 (GRCm39)	missense	probably benign	0.01
IGL02600:Vmn2r96	APN	17	18,817,829 (GRCm39)	missense	probably benign
IGL02672:Vmn2r96	APN	17	18,818,376 (GRCm39)	missense	probably benign	0.02
IGL02750:Vmn2r96	APN	17	18,802,851 (GRCm39)	missense	probably benign	0.01
IGL03068:Vmn2r96	APN	17	18,803,137 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r96	APN	17	18,806,634 (GRCm39)	missense	possibly damaging	0.88
R0316:Vmn2r96	UTSW	17	18,802,827 (GRCm39)	missense	probably damaging	1.00
R0502:Vmn2r96	UTSW	17	18,804,262 (GRCm39)	missense	probably benign
R0580:Vmn2r96	UTSW	17	18,802,900 (GRCm39)	missense	probably damaging	1.00
R0652:Vmn2r96	UTSW	17	18,817,830 (GRCm39)	missense	probably benign	0.05
R0789:Vmn2r96	UTSW	17	18,802,738 (GRCm39)	missense	possibly damaging	0.81
R1462:Vmn2r96	UTSW	17	18,817,660 (GRCm39)	missense	possibly damaging	0.94
R1462:Vmn2r96	UTSW	17	18,817,660 (GRCm39)	missense	possibly damaging	0.94
R1660:Vmn2r96	UTSW	17	18,817,988 (GRCm39)	missense	probably benign	0.00
R1755:Vmn2r96	UTSW	17	18,802,915 (GRCm39)	missense	possibly damaging	0.50
R1843:Vmn2r96	UTSW	17	18,818,183 (GRCm39)	missense	probably benign	0.14
R1943:Vmn2r96	UTSW	17	18,806,664 (GRCm39)	missense	probably benign	0.09
R1993:Vmn2r96	UTSW	17	18,804,138 (GRCm39)	missense	probably damaging	1.00
R2018:Vmn2r96	UTSW	17	18,804,263 (GRCm39)	missense	probably benign
R2405:Vmn2r96	UTSW	17	18,818,102 (GRCm39)	missense	probably damaging	0.96
R3977:Vmn2r96	UTSW	17	18,817,941 (GRCm39)	missense	probably damaging	1.00
R3979:Vmn2r96	UTSW	17	18,817,941 (GRCm39)	missense	probably damaging	1.00
R4059:Vmn2r96	UTSW	17	18,818,339 (GRCm39)	missense	probably benign	0.20
R4693:Vmn2r96	UTSW	17	18,803,270 (GRCm39)	missense	probably benign	0.03
R4709:Vmn2r96	UTSW	17	18,803,088 (GRCm39)	missense	probably benign	0.36
R4776:Vmn2r96	UTSW	17	18,817,770 (GRCm39)	missense	probably damaging	1.00
R4882:Vmn2r96	UTSW	17	18,817,866 (GRCm39)	missense	probably damaging	1.00
R4920:Vmn2r96	UTSW	17	18,802,918 (GRCm39)	missense	probably benign	0.07
R5143:Vmn2r96	UTSW	17	18,804,120 (GRCm39)	missense	possibly damaging	0.65
R5301:Vmn2r96	UTSW	17	18,817,950 (GRCm39)	missense	probably damaging	0.98
R5507:Vmn2r96	UTSW	17	18,818,091 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r96	UTSW	17	18,804,221 (GRCm39)	missense	probably benign	0.01
R6181:Vmn2r96	UTSW	17	18,804,126 (GRCm39)	missense	probably benign
R6339:Vmn2r96	UTSW	17	18,804,124 (GRCm39)	missense	possibly damaging	0.84
R6404:Vmn2r96	UTSW	17	18,817,793 (GRCm39)	missense	probably damaging	1.00
R6452:Vmn2r96	UTSW	17	18,804,117 (GRCm39)	missense	probably benign	0.07
R6749:Vmn2r96	UTSW	17	18,818,352 (GRCm39)	missense	probably damaging	0.99
R6813:Vmn2r96	UTSW	17	18,802,116 (GRCm39)	missense	probably benign	0.04
R6944:Vmn2r96	UTSW	17	18,817,891 (GRCm39)	missense	probably benign	0.16
R6949:Vmn2r96	UTSW	17	18,818,100 (GRCm39)	missense	probably damaging	1.00
R6962:Vmn2r96	UTSW	17	18,818,283 (GRCm39)	missense	probably damaging	0.99
R6990:Vmn2r96	UTSW	17	18,804,082 (GRCm39)	missense	probably benign
R7149:Vmn2r96	UTSW	17	18,817,989 (GRCm39)	missense	possibly damaging	0.89
R7346:Vmn2r96	UTSW	17	18,803,029 (GRCm39)	missense	probably benign	0.15
R7385:Vmn2r96	UTSW	17	18,803,302 (GRCm39)	missense	probably damaging	1.00
R7442:Vmn2r96	UTSW	17	18,793,662 (GRCm39)	missense	probably benign	0.02
R7509:Vmn2r96	UTSW	17	18,802,995 (GRCm39)	missense	probably benign	0.02
R7652:Vmn2r96	UTSW	17	18,793,832 (GRCm39)	missense	probably benign
R7659:Vmn2r96	UTSW	17	18,793,749 (GRCm39)	missense	probably benign	0.00
R7753:Vmn2r96	UTSW	17	18,806,663 (GRCm39)	missense	possibly damaging	0.46
R7855:Vmn2r96	UTSW	17	18,818,130 (GRCm39)	missense	possibly damaging	0.59
R8166:Vmn2r96	UTSW	17	18,802,744 (GRCm39)	missense	probably damaging	1.00
R8260:Vmn2r96	UTSW	17	18,804,243 (GRCm39)	missense	probably benign	0.04
R8323:Vmn2r96	UTSW	17	18,803,023 (GRCm39)	missense	probably damaging	0.99
R8787:Vmn2r96	UTSW	17	18,818,250 (GRCm39)	missense	probably damaging	1.00
R8837:Vmn2r96	UTSW	17	18,802,888 (GRCm39)	missense	probably benign
R8933:Vmn2r96	UTSW	17	18,804,241 (GRCm39)	missense	probably benign	0.11
R9306:Vmn2r96	UTSW	17	18,803,226 (GRCm39)	missense	probably benign	0.00
R9481:Vmn2r96	UTSW	17	18,793,621 (GRCm39)	start gained	probably benign
R9626:Vmn2r96	UTSW	17	18,793,758 (GRCm39)	missense	probably benign	0.14
R9629:Vmn2r96	UTSW	17	18,803,257 (GRCm39)	missense	probably benign	0.15
Z1088:Vmn2r96	UTSW	17	18,817,628 (GRCm39)	missense	possibly damaging	0.86
Z1177:Vmn2r96	UTSW	17	18,818,376 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- TCTCCTGAGTGACCATGGTC -3'
(R):5'- AGGCTCCCATGGAATGAGTC -3'

Sequencing Primer
(F):5'- GACCATGGTCAGTATCCTTCTC -3'
(R):5'- GCTCCCATGGAATGAGTCTAACATG -3'

Posted On

2018-09-12