|Institutional Source||Beutler Lab|
|Gene Name||coagulation factor III|
|Synonyms||TF, Cf3, tissue factor, Cf-3, CD142|
|Is this an essential gene?||Probably non essential (E-score: 0.087)|
|Stock #||R6867 (G1)|
|Chromosomal Location||121723537-121735048 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 121729371 bp|
|Amino Acid Change||Serine to Proline at position 77 (S77P)|
|Ref Sequence||ENSEMBL: ENSMUSP00000029771 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000029771]|
|Predicted Effect||possibly damaging
AA Change: S77P
PolyPhen 2 Score 0.928 (Sensitivity: 0.81; Specificity: 0.94)
AA Change: S77P
|Predicted Effect||probably benign
|Coding Region Coverage||
|Validation Efficiency||100% (43/43)|
FUNCTION: This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]
PHENOTYPE: Homozygotes for targeted null mutations exhibit impaired blood vessel development, retarded growth, and, in most cases, midgestational lethality. On a mixed background, some mutants survive to birth and appear to be normal. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in F3||
(F):5'- AGAGCTAACCTGGTTACTGGG -3'
(R):5'- GTGAAGGCATTACAAACCAAGC -3'
(F):5'- AGCTAACCTGGTTACTGGGTGAAC -3'
(R):5'- AACCAAGCTACTTACTGTCTCGG -3'