|Institutional Source||Beutler Lab|
|Gene Name||replication factor C (activator 1) 3|
|Synonyms||2810416I22Rik, Recc3, 38kDa, 38kDa|
|Is this an essential gene?||Probably essential (E-score: 0.944)|
|Stock #||R6883 (G1)|
|Chromosomal Location||151642756-151651242 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 151648284 bp|
|Amino Acid Change||Serine to Asparagine at position 85 (S85N)|
|Ref Sequence||ENSEMBL: ENSMUSP00000039621 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000038131]|
|Predicted Effect||probably benign
AA Change: S85N
PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
AA Change: S85N
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rfc3||
(F):5'- ACTGACCTAAGGGACCTCTG -3'
(R):5'- GATCGCAAAGACACATTATTCAGTG -3'
(F):5'- GGGACCTCTGACAACAGTATTTTAG -3'
(R):5'- GACTGTAGATAGTTGGCAG -3'