Mutagenetix > Incidental Mutation

Incidental Mutation 'R6838:Mapk13'

537986

Institutional Source

Beutler Lab

Gene Symbol

Mapk13

Ensembl Gene

ENSMUSG00000004864

Gene Name

mitogen-activated protein kinase 13

Synonyms

p38 delta MAP kinase, SAPK4, Serk4

MMRRC Submission

044946-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.199) question?

Stock #

R6838 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

28988260-28997678 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to T at 28996535 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000004986 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004986] [ENSMUST00000129096]

AlphaFold

Q9Z1B7

Predicted Effect

probably null
Transcript: ENSMUST00000004986

SMART Domains

Protein: ENSMUSP00000004986
Gene: ENSMUSG00000004864

Domain	Start	End	E-Value	Type
S_TKc	25	308	8.72e-97	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000129096

SMART Domains

Protein: ENSMUSP00000115659
Gene: ENSMUSG00000004864

Domain	Start	End	E-Value	Type
Pfam:Pkinase	25	209	1.1e-49	PFAM
Pfam:Pkinase_Tyr	27	210	2e-24	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.7%

Validation Efficiency

97% (64/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the mitogen-activated protein (MAP) kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The encoded protein is a p38 MAP kinase and is activated by proinflammatory cytokines and cellular stress. Substrates of the encoded protein include the transcription factor ATF2 and the microtubule dynamics regulator stathmin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
PHENOTYPE: Mice homozygous for a null allele exhibit improved glucose tolerance, increased insulin secretion, decreased blood glucose, and decreased susceptibility to diet- or chemically-induced diabetes. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aim2	C	G	1: 173,291,546 (GRCm39)	T317R	probably damaging	Het
Aqp9	T	A	9: 71,019,498 (GRCm39)	M321L	probably benign	Het
Avil	G	A	10: 126,849,431 (GRCm39)	D576N	probably benign	Het
Bbs1	C	T	19: 4,953,880 (GRCm39)	M94I	possibly damaging	Het
Bms1	A	G	6: 118,393,455 (GRCm39)	V139A	probably benign	Het
Bscl2	A	T	19: 8,818,745 (GRCm39)	M57L	probably damaging	Het
C2cd5	A	G	6: 142,975,364 (GRCm39)	I794T	possibly damaging	Het
Cacna1s	C	T	1: 136,012,175 (GRCm39)	T539I	possibly damaging	Het
Cand1	T	C	10: 119,045,935 (GRCm39)	K990R	probably benign	Het
Capn7	A	G	14: 31,076,130 (GRCm39)	I311V	possibly damaging	Het
Cd180	A	G	13: 102,839,239 (GRCm39)	N41D	probably benign	Het
Cdin1	T	C	2: 115,607,471 (GRCm39)	F275L	possibly damaging	Het
Celsr1	G	A	15: 85,823,395 (GRCm39)	T1671I	probably benign	Het
Cep135	A	T	5: 76,780,062 (GRCm39)	Q798L	probably damaging	Het
Cfap65	T	C	1: 74,971,180 (GRCm39)	D46G	probably benign	Het
Cracd	A	T	5: 77,006,056 (GRCm39)	T806S	unknown	Het
Ddx46	G	T	13: 55,787,748 (GRCm39)		probably null	Het
Dnah7b	T	C	1: 46,230,948 (GRCm39)	L1402P	probably damaging	Het
Dnah8	A	G	17: 30,929,525 (GRCm39)	E1402G	probably damaging	Het
Dock9	A	G	14: 121,784,008 (GRCm39)	Y1989H	possibly damaging	Het
Ereg	A	G	5: 91,236,323 (GRCm39)	D50G	probably benign	Het
Evi5	G	T	5: 107,990,027 (GRCm39)	T64K	possibly damaging	Het
Frem3	A	C	8: 81,338,660 (GRCm39)	T318P	probably damaging	Het
Gpx1	A	G	9: 108,217,139 (GRCm39)	D81G	possibly damaging	Het
Gramd1a	T	C	7: 30,833,929 (GRCm39)	I499V	probably benign	Het
H4c12	A	T	13: 21,934,375 (GRCm39)	F101I	probably damaging	Het
Herc2	T	A	7: 55,758,526 (GRCm39)	D804E	probably damaging	Het
Hk1	T	C	10: 62,107,437 (GRCm39)	E846G	probably damaging	Het
Iars2	G	A	1: 185,061,342 (GRCm39)	A48V	probably damaging	Het
Invs	C	T	4: 48,283,278 (GRCm39)	T10M	possibly damaging	Het
Ism2	A	T	12: 87,326,975 (GRCm39)	D321E	probably benign	Het
Itpr1	T	C	6: 108,448,152 (GRCm39)	S231P	possibly damaging	Het
Kif17	A	T	4: 138,005,710 (GRCm39)		probably null	Het
Lvrn	A	G	18: 47,023,947 (GRCm39)	I765V	possibly damaging	Het
Map4k4	T	A	1: 40,015,882 (GRCm39)	C108S	probably damaging	Het
Mapkapk2	T	A	1: 130,985,740 (GRCm39)	K95*	probably null	Het
Mau2	A	T	8: 70,491,947 (GRCm39)		probably null	Het
Mex3a	A	G	3: 88,444,084 (GRCm39)	T387A	probably benign	Het
Myo5a	T	C	9: 75,061,165 (GRCm39)		probably null	Het
Ncbp3	A	T	11: 72,964,300 (GRCm39)	M417L	possibly damaging	Het
Nod2	A	C	8: 89,397,086 (GRCm39)	E810A	possibly damaging	Het
Nol3	A	T	8: 106,006,207 (GRCm39)	E152V	probably damaging	Het
Obox6	T	C	7: 15,567,664 (GRCm39)	E261G	possibly damaging	Het
Or2h1	A	T	17: 37,404,058 (GRCm39)	L236*	probably null	Het
Or8b12b	A	C	9: 37,684,348 (GRCm39)	Y131S	possibly damaging	Het
P3h2	T	C	16: 25,924,034 (GRCm39)	S134G	possibly damaging	Het
Plxna2	A	T	1: 194,487,222 (GRCm39)	R1592S	possibly damaging	Het
Ppp1r12a	C	T	10: 108,097,137 (GRCm39)	S250L	possibly damaging	Het
Rab38	A	G	7: 88,099,917 (GRCm39)	D144G	possibly damaging	Het
Septin1	T	C	7: 126,815,894 (GRCm39)	M176V	probably benign	Het
Spata22	C	T	11: 73,236,759 (GRCm39)	T355M	probably benign	Het
Tango6	A	G	8: 107,468,706 (GRCm39)	N734S	probably benign	Het
Tbc1d17	C	A	7: 44,493,738 (GRCm39)	R295L	probably damaging	Het
Thsd7a	G	T	6: 12,504,074 (GRCm39)	P360Q	probably damaging	Het
Tmem161b	C	A	13: 84,370,537 (GRCm39)		probably benign	Het
Tnnt1	T	C	7: 4,510,406 (GRCm39)	N239S	possibly damaging	Het
Tpst1	A	T	5: 130,131,279 (GRCm39)	M250L	probably benign	Het
Urb1	T	A	16: 90,578,994 (GRCm39)	D689V	possibly damaging	Het
Usp28	T	A	9: 48,911,730 (GRCm39)		probably null	Het
Vldlr	G	A	19: 27,225,370 (GRCm39)	D816N	probably damaging	Het
Vmn1r257	T	A	7: 22,391,142 (GRCm39)	M201L	probably benign	Het
Wdr1	A	G	5: 38,687,374 (GRCm39)	V219A	probably damaging	Het
Xndc1	T	C	7: 101,722,476 (GRCm39)	V47A	possibly damaging	Het
Zfp366	C	T	13: 99,365,015 (GRCm39)	P59S	possibly damaging	Het
Zfp366	A	G	13: 99,382,685 (GRCm39)	E616G	possibly damaging	Het
Zfp937	T	A	2: 150,081,266 (GRCm39)	I432K	probably benign	Het

Other mutations in Mapk13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00329:Mapk13	APN	17	28,995,379 (GRCm39)	missense	probably damaging	1.00
IGL01918:Mapk13	APN	17	28,994,304 (GRCm39)	missense	probably damaging	1.00
IGL02265:Mapk13	APN	17	28,996,692 (GRCm39)	splice site	probably benign
IGL02451:Mapk13	APN	17	28,995,387 (GRCm39)	missense	probably damaging	1.00
IGL02977:Mapk13	APN	17	28,995,322 (GRCm39)	missense	probably damaging	1.00
IGL03118:Mapk13	APN	17	28,996,709 (GRCm39)	missense	probably benign	0.14
IGL03188:Mapk13	APN	17	28,995,557 (GRCm39)	intron	probably benign
R0501:Mapk13	UTSW	17	28,995,327 (GRCm39)	missense	probably damaging	1.00
R0538:Mapk13	UTSW	17	28,994,229 (GRCm39)	missense	probably damaging	1.00
R2240:Mapk13	UTSW	17	28,997,085 (GRCm39)	missense	probably damaging	0.98
R4368:Mapk13	UTSW	17	28,996,539 (GRCm39)	splice site	probably null
R4613:Mapk13	UTSW	17	28,988,426 (GRCm39)	missense	probably damaging	1.00
R4649:Mapk13	UTSW	17	28,997,461 (GRCm39)	nonsense	probably null
R4684:Mapk13	UTSW	17	28,989,023 (GRCm39)	missense	probably damaging	1.00
R4796:Mapk13	UTSW	17	28,994,528 (GRCm39)	missense	probably damaging	1.00
R4863:Mapk13	UTSW	17	28,995,284 (GRCm39)	missense	probably damaging	1.00
R4923:Mapk13	UTSW	17	28,997,197 (GRCm39)	missense	probably benign
R5220:Mapk13	UTSW	17	28,997,465 (GRCm39)	missense	probably benign	0.00
R5247:Mapk13	UTSW	17	28,996,725 (GRCm39)	missense	probably benign	0.01
R5370:Mapk13	UTSW	17	28,995,326 (GRCm39)	nonsense	probably null
R6843:Mapk13	UTSW	17	28,994,427 (GRCm39)	splice site	probably null
R7187:Mapk13	UTSW	17	28,995,361 (GRCm39)	missense	probably damaging	1.00
R9018:Mapk13	UTSW	17	28,996,760 (GRCm39)	missense	probably benign
R9227:Mapk13	UTSW	17	28,994,532 (GRCm39)	missense	probably damaging	1.00
R9230:Mapk13	UTSW	17	28,994,532 (GRCm39)	missense	probably damaging	1.00
R9241:Mapk13	UTSW	17	28,990,187 (GRCm39)	missense	probably damaging	0.99
R9249:Mapk13	UTSW	17	28,988,490 (GRCm39)	missense	probably damaging	1.00
R9274:Mapk13	UTSW	17	28,988,490 (GRCm39)	missense	probably damaging	1.00
R9777:Mapk13	UTSW	17	28,997,075 (GRCm39)	missense	probably damaging	1.00
Z1088:Mapk13	UTSW	17	28,996,507 (GRCm39)	missense	possibly damaging	0.48

Predicted Primers

PCR Primer
(F):5'- AAGTAGCCTCCTTCTGACCTGG -3'
(R):5'- AGGATTTGGCCTGGAAGAC -3'

Sequencing Primer
(F):5'- AAGAGCCTGCCGTGGTTAG -3'
(R):5'- ACAGAAAAATATGGAATGTCTTCAGG -3'

Posted On

2018-10-18