Mutagenetix > Incidental Mutation

Incidental Mutation 'R6919:Ascc3'

539509

Institutional Source

Beutler Lab

Gene Symbol

Ascc3

Ensembl Gene

ENSMUSG00000038774

Gene Name

activating signal cointegrator 1 complex subunit 3

Synonyms

Helic1, B630009I04Rik, ASC1p200

MMRRC Submission

045039-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.963) question?

Stock #

R6919 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

50468756-50727300 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 50521849 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 455 (E455*)

Ref Sequence

ENSEMBL: ENSMUSP00000036726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035606] [ENSMUST00000220355]

AlphaFold

E9PZJ8

Predicted Effect

probably null
Transcript: ENSMUST00000035606
AA Change: E455*

SMART Domains

Protein: ENSMUSP00000036726
Gene: ENSMUSG00000038774
AA Change: E455*

Domain	Start	End	E-Value	Type
coiled coil region	55	79	N/A	INTRINSIC
low complexity region	124	135	N/A	INTRINSIC
coiled coil region	329	356	N/A	INTRINSIC
DEXDc	474	686	1.71e-29	SMART
AAA	492	674	8.15e-2	SMART
Blast:DEXDc	718	763	4e-18	BLAST
HELICc	770	858	6.01e-16	SMART
Sec63	979	1288	3.53e-111	SMART
DEXDc	1324	1528	8.88e-28	SMART
AAA	1342	1492	4.27e-1	SMART
HELICc	1605	1695	2.28e-16	SMART
Sec63	1813	2178	6.37e-118	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000220355

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.8%

Validation Efficiency

99% (84/85)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to a family of helicases that are involved in the ATP-dependent unwinding of nucleic acid duplexes. The encoded protein is the largest subunit of the activating signal cointegrator 1 complex that is involved in DNA repair and resistance to alkylation damage. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

All alleles(16) : Targeted(2) Gene trapped(14)

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700086D15Rik	A	G	11: 65,043,356 (GRCm39)		probably benign	Het
6430548M08Rik	T	C	8: 120,872,221 (GRCm39)	S50P	probably damaging	Het
Aacs	A	G	5: 125,583,227 (GRCm39)	D261G	probably benign	Het
Abcc4	T	A	14: 118,832,306 (GRCm39)	T775S	probably benign	Het
Acsl6	A	G	11: 54,232,582 (GRCm39)		probably null	Het
Agbl5	T	A	5: 31,062,061 (GRCm39)	F196I	probably benign	Het
Ahnak2	T	C	12: 112,741,118 (GRCm39)	T985A	possibly damaging	Het
Ak1	A	T	2: 32,521,134 (GRCm39)	D101V	possibly damaging	Het
Alx1	A	G	10: 102,861,061 (GRCm39)	Y156H	probably damaging	Het
Angptl7	T	A	4: 148,584,488 (GRCm39)	S87C	probably benign	Het
Ankrd36	A	G	11: 5,579,299 (GRCm39)	T188A	probably benign	Het
Arhgap11a	A	C	2: 113,670,054 (GRCm39)	S356R	possibly damaging	Het
Atp6v0a2	G	A	5: 124,789,225 (GRCm39)		probably null	Het
B3gnt7	T	C	1: 86,233,416 (GRCm39)	W104R	probably damaging	Het
Bbs9	G	A	9: 22,723,840 (GRCm39)		probably null	Het
Cc2d2a	T	A	5: 43,860,557 (GRCm39)	D544E	probably benign	Het
Cic	C	T	7: 24,971,202 (GRCm39)	T311I	probably benign	Het
Cngb1	T	A	8: 95,975,003 (GRCm39)	R1157W	probably null	Het
Cntln	A	G	4: 85,033,605 (GRCm39)	H1310R	probably benign	Het
Cntnap5c	A	T	17: 58,600,948 (GRCm39)	I764F	probably benign	Het
Col26a1	T	C	5: 136,773,088 (GRCm39)	Q362R	possibly damaging	Het
Cyp2c29	G	A	19: 39,279,585 (GRCm39)	R100K	probably benign	Het
D17H6S53E	C	G	17: 35,346,222 (GRCm39)	D44E	probably damaging	Het
Dap3	A	G	3: 88,838,296 (GRCm39)	V65A	probably damaging	Het
Dna2	A	T	10: 62,792,782 (GRCm39)	I266F	probably damaging	Het
Dnah14	G	A	1: 181,412,631 (GRCm39)	G57E	probably benign	Het
Dock9	A	T	14: 121,880,564 (GRCm39)	V333E	probably benign	Het
Dpm1	A	G	2: 168,072,195 (GRCm39)	Y27H	probably damaging	Het
Dsp	A	T	13: 38,351,631 (GRCm39)	Y150F	possibly damaging	Het
Emilin3	A	T	2: 160,750,018 (GRCm39)	I577N	probably damaging	Het
Erap1	A	G	13: 74,819,552 (GRCm39)	T189A	probably benign	Het
Fat2	A	G	11: 55,173,597 (GRCm39)	I2372T	possibly damaging	Het
Fbn2	A	T	18: 58,257,259 (GRCm39)		probably null	Het
Gnl1	A	T	17: 36,298,425 (GRCm39)	R390*	probably null	Het
Hivep1	A	G	13: 42,336,928 (GRCm39)	I2336V	probably benign	Het
Il17rb	C	G	14: 29,726,228 (GRCm39)		probably null	Het
Itga9	T	C	9: 118,716,883 (GRCm39)	W396R	probably damaging	Het
Katnal2	A	G	18: 77,098,734 (GRCm39)	V152A	probably benign	Het
Kcnk3	A	G	5: 30,779,744 (GRCm39)	T265A	probably benign	Het
Klhl1	T	C	14: 96,374,030 (GRCm39)	Y672C	probably benign	Het
Leng8	C	A	7: 4,146,625 (GRCm39)	N412K	possibly damaging	Het
Lrrc9	T	A	12: 72,553,167 (GRCm39)	F1356L	probably benign	Het
Map7	A	G	10: 20,046,828 (GRCm39)		probably benign	Het
Mei1	T	C	15: 81,966,131 (GRCm39)	F251S	probably damaging	Het
Mia2	A	G	12: 59,176,681 (GRCm39)	E9G	possibly damaging	Het
Ms4a13	C	A	19: 11,149,249 (GRCm39)	W182C	probably benign	Het
Muc16	C	T	9: 18,571,595 (GRCm39)	R308K	unknown	Het
Or4c103	G	A	2: 88,514,028 (GRCm39)	T16I	possibly damaging	Het
Or8g22	A	G	9: 38,958,827 (GRCm39)		probably benign	Het
Pcnt	A	G	10: 76,221,632 (GRCm39)	V1998A	probably benign	Het
Pgm2	A	T	5: 64,254,368 (GRCm39)	N51I	probably benign	Het
Piezo1	T	A	8: 123,217,020 (GRCm39)	H1333L	probably damaging	Het
Prg2	G	A	2: 84,813,600 (GRCm39)	V199M	probably damaging	Het
Prss51	T	C	14: 64,334,937 (GRCm39)	V182A	probably damaging	Het
Psmb9	A	T	17: 34,402,199 (GRCm39)	Y132*	probably null	Het
Ralyl	T	C	3: 13,842,091 (GRCm39)	Y76H	probably damaging	Het
Rnaset2a	A	T	17: 8,349,114 (GRCm39)	D174E	probably benign	Het
Rnft1	G	A	11: 86,386,156 (GRCm39)		probably null	Het
Robo2	T	C	16: 73,758,755 (GRCm39)	Y676C	probably damaging	Het
Samd9l	T	G	6: 3,376,313 (GRCm39)	Y316S	possibly damaging	Het
Siah3	T	A	14: 75,693,578 (GRCm39)	F28Y	possibly damaging	Het
Slc28a3	C	T	13: 58,721,257 (GRCm39)		probably null	Het
Slc8a1	A	G	17: 81,696,301 (GRCm39)	F911S	probably damaging	Het
Spata31e2	T	C	1: 26,722,015 (GRCm39)	Y1055C	probably benign	Het
Speg	T	A	1: 75,364,552 (GRCm39)	L156*	probably null	Het
Spopl	T	G	2: 23,407,873 (GRCm39)	M269L	probably benign	Het
Tacr1	C	T	6: 82,534,054 (GRCm39)	T360I	probably benign	Het
Tasor	T	A	14: 27,171,758 (GRCm39)	L397*	probably null	Het
Tmem262	A	G	19: 6,130,767 (GRCm39)	E95G	probably benign	Het
Tmem68	G	T	4: 3,569,669 (GRCm39)	T7N	possibly damaging	Het
Top3a	T	A	11: 60,640,319 (GRCm39)	I460F	probably damaging	Het
Trafd1	G	A	5: 121,522,137 (GRCm39)	R5*	probably null	Het
Trim16	T	A	11: 62,731,695 (GRCm39)	V435D	possibly damaging	Het
Tspan17	A	G	13: 54,943,846 (GRCm39)	D236G	probably damaging	Het
Tssk2	A	T	16: 17,717,565 (GRCm39)	M323L	probably benign	Het
Ubqln5	A	G	7: 103,778,215 (GRCm39)	V203A	probably benign	Het
Utrn	A	T	10: 12,569,214 (GRCm39)	L1134*	probably null	Het
Vmn1r37	C	T	6: 66,708,704 (GRCm39)	S73F	possibly damaging	Het
Vps13c	A	G	9: 67,834,734 (GRCm39)	K1688E	probably damaging	Het
Zbtb39	A	G	10: 127,577,711 (GRCm39)	D95G	probably damaging	Het
Zfhx3	T	A	8: 109,527,160 (GRCm39)	I1019N	probably damaging	Het
Zfp207	C	T	11: 80,286,329 (GRCm39)		probably benign	Het
Zmiz1	C	T	14: 25,644,062 (GRCm39)	T169M	probably damaging	Het

Other mutations in Ascc3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00264:Ascc3	APN	10	50,590,531 (GRCm39)	missense	probably damaging	0.99
IGL00690:Ascc3	APN	10	50,576,039 (GRCm39)	nonsense	probably null
IGL00897:Ascc3	APN	10	50,604,187 (GRCm39)	missense	probably benign	0.01
IGL01077:Ascc3	APN	10	50,525,413 (GRCm39)	splice site	probably benign
IGL01124:Ascc3	APN	10	50,608,569 (GRCm39)	missense	probably damaging	1.00
IGL01555:Ascc3	APN	10	50,626,618 (GRCm39)	missense	probably damaging	1.00
IGL02019:Ascc3	APN	10	50,566,235 (GRCm39)	missense	probably damaging	1.00
IGL02161:Ascc3	APN	10	50,726,623 (GRCm39)	nonsense	probably null
IGL02247:Ascc3	APN	10	50,526,686 (GRCm39)	missense	probably damaging	1.00
IGL02318:Ascc3	APN	10	50,604,250 (GRCm39)	nonsense	probably null
IGL02428:Ascc3	APN	10	50,721,791 (GRCm39)	nonsense	probably null
IGL02432:Ascc3	APN	10	50,576,589 (GRCm39)	missense	probably damaging	0.99
IGL02449:Ascc3	APN	10	50,576,695 (GRCm39)	missense	probably benign	0.00
IGL02640:Ascc3	APN	10	50,643,470 (GRCm39)	missense	possibly damaging	0.69
IGL02673:Ascc3	APN	10	50,536,769 (GRCm39)	missense	probably benign	0.01
IGL03144:Ascc3	APN	10	50,643,539 (GRCm39)	missense	probably benign	0.16
IGL03161:Ascc3	APN	10	50,494,168 (GRCm39)	missense	probably damaging	0.98
IGL03218:Ascc3	APN	10	50,699,949 (GRCm39)	missense	possibly damaging	0.89
algorithm	UTSW	10	50,594,472 (GRCm39)	missense	probably damaging	0.97
heuristic	UTSW	10	50,718,289 (GRCm39)	missense	probably damaging	0.99
network	UTSW	10	50,630,175 (GRCm39)	missense	possibly damaging	0.53
R0045:Ascc3	UTSW	10	50,594,498 (GRCm39)	nonsense	probably null
R0045:Ascc3	UTSW	10	50,594,498 (GRCm39)	nonsense	probably null
R0131:Ascc3	UTSW	10	50,611,425 (GRCm39)	missense	probably damaging	0.99
R0131:Ascc3	UTSW	10	50,611,425 (GRCm39)	missense	probably damaging	0.99
R0132:Ascc3	UTSW	10	50,611,425 (GRCm39)	missense	probably damaging	0.99
R0149:Ascc3	UTSW	10	50,484,089 (GRCm39)	missense	probably benign	0.31
R0165:Ascc3	UTSW	10	50,718,223 (GRCm39)	splice site	probably null
R0255:Ascc3	UTSW	10	50,521,154 (GRCm39)	missense	probably benign	0.00
R0310:Ascc3	UTSW	10	50,625,022 (GRCm39)	missense	probably benign	0.02
R0314:Ascc3	UTSW	10	50,514,095 (GRCm39)	missense	possibly damaging	0.92
R0362:Ascc3	UTSW	10	50,625,051 (GRCm39)	splice site	probably benign
R0418:Ascc3	UTSW	10	50,625,022 (GRCm39)	missense	probably benign	0.02
R0419:Ascc3	UTSW	10	50,625,022 (GRCm39)	missense	probably benign	0.02
R0421:Ascc3	UTSW	10	50,625,022 (GRCm39)	missense	probably benign	0.02
R0480:Ascc3	UTSW	10	50,611,348 (GRCm39)	missense	probably damaging	1.00
R0744:Ascc3	UTSW	10	50,721,762 (GRCm39)	missense	probably benign	0.17
R0833:Ascc3	UTSW	10	50,721,762 (GRCm39)	missense	probably benign	0.17
R1231:Ascc3	UTSW	10	50,699,756 (GRCm39)	missense	probably damaging	1.00
R1264:Ascc3	UTSW	10	50,518,615 (GRCm39)	splice site	probably benign
R1302:Ascc3	UTSW	10	50,480,890 (GRCm39)	start codon destroyed	probably null	1.00
R1751:Ascc3	UTSW	10	50,594,472 (GRCm39)	missense	probably damaging	0.97
R1767:Ascc3	UTSW	10	50,594,472 (GRCm39)	missense	probably damaging	0.97
R1769:Ascc3	UTSW	10	50,576,586 (GRCm39)	missense	probably damaging	1.00
R1840:Ascc3	UTSW	10	50,566,257 (GRCm39)	missense	probably benign	0.00
R1855:Ascc3	UTSW	10	50,494,018 (GRCm39)	missense	probably benign	0.01
R1953:Ascc3	UTSW	10	50,721,726 (GRCm39)	missense	probably benign
R1976:Ascc3	UTSW	10	50,525,262 (GRCm39)	missense	probably damaging	1.00
R2004:Ascc3	UTSW	10	50,493,838 (GRCm39)	missense	probably damaging	1.00
R2013:Ascc3	UTSW	10	50,525,908 (GRCm39)	missense	probably damaging	0.99
R2017:Ascc3	UTSW	10	50,566,307 (GRCm39)	missense	probably benign	0.00
R2040:Ascc3	UTSW	10	50,604,227 (GRCm39)	missense	probably benign
R2043:Ascc3	UTSW	10	50,576,616 (GRCm39)	missense	probably damaging	1.00
R2165:Ascc3	UTSW	10	50,597,935 (GRCm39)	missense	probably damaging	1.00
R2226:Ascc3	UTSW	10	50,630,148 (GRCm39)	missense	probably benign	0.07
R2310:Ascc3	UTSW	10	50,624,988 (GRCm39)	missense	probably benign	0.15
R2405:Ascc3	UTSW	10	50,607,774 (GRCm39)	missense	probably damaging	1.00
R2424:Ascc3	UTSW	10	50,494,297 (GRCm39)	missense	probably benign	0.14
R3410:Ascc3	UTSW	10	50,576,196 (GRCm39)	missense	probably damaging	1.00
R3617:Ascc3	UTSW	10	50,494,281 (GRCm39)	missense	probably benign	0.00
R3771:Ascc3	UTSW	10	50,596,814 (GRCm39)	splice site	probably benign
R3783:Ascc3	UTSW	10	50,604,350 (GRCm39)	missense	probably damaging	1.00
R3891:Ascc3	UTSW	10	50,718,289 (GRCm39)	missense	probably damaging	0.99
R3892:Ascc3	UTSW	10	50,718,289 (GRCm39)	missense	probably damaging	0.99
R4435:Ascc3	UTSW	10	50,597,981 (GRCm39)	missense	probably benign	0.14
R4509:Ascc3	UTSW	10	50,718,339 (GRCm39)	missense	probably benign	0.00
R4520:Ascc3	UTSW	10	50,536,766 (GRCm39)	missense	probably benign
R4521:Ascc3	UTSW	10	50,536,766 (GRCm39)	missense	probably benign
R4522:Ascc3	UTSW	10	50,536,766 (GRCm39)	missense	probably benign
R4524:Ascc3	UTSW	10	50,536,766 (GRCm39)	missense	probably benign
R4581:Ascc3	UTSW	10	50,587,121 (GRCm39)	missense	probably damaging	1.00
R4701:Ascc3	UTSW	10	50,596,760 (GRCm39)	missense	possibly damaging	0.66
R4704:Ascc3	UTSW	10	50,535,110 (GRCm39)	missense	probably benign	0.02
R4768:Ascc3	UTSW	10	50,576,595 (GRCm39)	missense	probably damaging	1.00
R4823:Ascc3	UTSW	10	50,589,329 (GRCm39)	missense	probably damaging	1.00
R4906:Ascc3	UTSW	10	50,625,227 (GRCm39)	missense	probably damaging	1.00
R4937:Ascc3	UTSW	10	50,699,894 (GRCm39)	missense	probably damaging	1.00
R5001:Ascc3	UTSW	10	50,699,744 (GRCm39)	missense	probably damaging	1.00
R5151:Ascc3	UTSW	10	50,514,059 (GRCm39)	missense	probably damaging	0.99
R5263:Ascc3	UTSW	10	50,592,757 (GRCm39)	missense	probably benign	0.00
R5302:Ascc3	UTSW	10	50,583,873 (GRCm39)	missense	probably benign	0.09
R5436:Ascc3	UTSW	10	50,535,079 (GRCm39)	missense	probably damaging	0.99
R5455:Ascc3	UTSW	10	50,725,679 (GRCm39)	missense	probably benign	0.06
R5474:Ascc3	UTSW	10	50,725,634 (GRCm39)	missense	probably benign	0.25
R5744:Ascc3	UTSW	10	50,586,977 (GRCm39)	missense	probably benign
R5781:Ascc3	UTSW	10	50,514,074 (GRCm39)	missense	probably damaging	1.00
R5850:Ascc3	UTSW	10	50,587,049 (GRCm39)	missense	probably damaging	1.00
R5867:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R5868:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R5869:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R6031:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R6031:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R6032:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R6032:Ascc3	UTSW	10	50,718,279 (GRCm39)	nonsense	probably null
R6109:Ascc3	UTSW	10	50,525,343 (GRCm39)	missense	probably benign	0.37
R6122:Ascc3	UTSW	10	50,494,021 (GRCm39)	missense	probably benign
R6128:Ascc3	UTSW	10	50,526,734 (GRCm39)	missense	probably damaging	1.00
R6351:Ascc3	UTSW	10	50,596,769 (GRCm39)	missense	probably damaging	0.99
R6368:Ascc3	UTSW	10	50,576,081 (GRCm39)	missense	probably damaging	1.00
R6369:Ascc3	UTSW	10	50,576,081 (GRCm39)	missense	probably damaging	1.00
R6409:Ascc3	UTSW	10	50,721,676 (GRCm39)	missense	probably benign	0.09
R6472:Ascc3	UTSW	10	50,596,783 (GRCm39)	missense	probably benign	0.03
R6474:Ascc3	UTSW	10	50,624,932 (GRCm39)	missense	probably benign	0.01
R6480:Ascc3	UTSW	10	50,587,049 (GRCm39)	missense	probably damaging	1.00
R6553:Ascc3	UTSW	10	50,718,273 (GRCm39)	missense	probably benign	0.05
R6572:Ascc3	UTSW	10	50,566,343 (GRCm39)	nonsense	probably null
R6585:Ascc3	UTSW	10	50,718,273 (GRCm39)	missense	probably benign	0.05
R6656:Ascc3	UTSW	10	50,526,021 (GRCm39)	nonsense	probably null
R6669:Ascc3	UTSW	10	50,716,469 (GRCm39)	missense	probably benign
R6675:Ascc3	UTSW	10	50,626,659 (GRCm39)	nonsense	probably null
R6790:Ascc3	UTSW	10	50,521,808 (GRCm39)	missense	probably damaging	1.00
R6856:Ascc3	UTSW	10	50,625,158 (GRCm39)	missense	probably damaging	1.00
R6862:Ascc3	UTSW	10	50,725,742 (GRCm39)	missense	probably null	0.51
R6936:Ascc3	UTSW	10	50,606,057 (GRCm39)	missense	probably damaging	0.98
R6953:Ascc3	UTSW	10	50,521,762 (GRCm39)	missense	probably benign	0.00
R6957:Ascc3	UTSW	10	50,604,278 (GRCm39)	missense	probably damaging	1.00
R7022:Ascc3	UTSW	10	50,592,725 (GRCm39)	missense	possibly damaging	0.55
R7050:Ascc3	UTSW	10	50,716,446 (GRCm39)	missense	probably benign	0.43
R7358:Ascc3	UTSW	10	50,590,448 (GRCm39)	nonsense	probably null
R7479:Ascc3	UTSW	10	50,525,895 (GRCm39)	missense	probably damaging	1.00
R7538:Ascc3	UTSW	10	50,721,796 (GRCm39)	missense	probably damaging	1.00
R7838:Ascc3	UTSW	10	50,604,393 (GRCm39)	missense	probably benign	0.04
R8021:Ascc3	UTSW	10	50,607,744 (GRCm39)	missense	probably benign	0.02
R8134:Ascc3	UTSW	10	50,643,554 (GRCm39)	missense	probably benign	0.02
R8252:Ascc3	UTSW	10	50,518,706 (GRCm39)	missense	probably benign
R8348:Ascc3	UTSW	10	50,494,173 (GRCm39)	missense	probably benign
R8351:Ascc3	UTSW	10	50,725,693 (GRCm39)	missense	probably benign
R8356:Ascc3	UTSW	10	50,526,003 (GRCm39)	missense	probably benign	0.38
R8362:Ascc3	UTSW	10	50,518,692 (GRCm39)	missense	possibly damaging	0.93
R8395:Ascc3	UTSW	10	50,525,400 (GRCm39)	missense	possibly damaging	0.93
R8448:Ascc3	UTSW	10	50,494,173 (GRCm39)	missense	probably benign
R8957:Ascc3	UTSW	10	50,576,208 (GRCm39)	missense	probably damaging	1.00
R9004:Ascc3	UTSW	10	50,718,276 (GRCm39)	missense	probably benign
R9133:Ascc3	UTSW	10	50,630,175 (GRCm39)	missense	possibly damaging	0.53
R9200:Ascc3	UTSW	10	50,521,787 (GRCm39)	missense	possibly damaging	0.55
R9328:Ascc3	UTSW	10	50,535,015 (GRCm39)	missense	probably damaging	1.00
R9377:Ascc3	UTSW	10	50,608,858 (GRCm39)	missense	possibly damaging	0.62
R9412:Ascc3	UTSW	10	50,525,230 (GRCm39)	missense	probably benign	0.00
R9576:Ascc3	UTSW	10	50,494,254 (GRCm39)	missense	possibly damaging	0.71
R9796:Ascc3	UTSW	10	50,608,801 (GRCm39)	nonsense	probably null
X0021:Ascc3	UTSW	10	50,576,686 (GRCm39)	missense	possibly damaging	0.88
X0025:Ascc3	UTSW	10	50,526,692 (GRCm39)	missense	probably benign	0.00
X0026:Ascc3	UTSW	10	50,608,574 (GRCm39)	missense	probably damaging	1.00
Z1177:Ascc3	UTSW	10	50,594,517 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACTCCTGAATGCTTTATCAGTATCG -3'
(R):5'- TTGTTTCATGTATAAGGAACTCGCG -3'

Sequencing Primer
(F):5'- CTTACCAGAGCAGTTGCT -3'
(R):5'- AGGAACTCGCGTACATATTTTTCACC -3'

Posted On

2018-11-06