Mutagenetix > Incidental Mutation

Incidental Mutation 'R6940:Muc21'

540519

Institutional Source

Beutler Lab

Gene Symbol

Muc21

Ensembl Gene

ENSMUSG00000090588

Gene Name

mucin 21

Synonyms

epiglycanin, Gm9573

MMRRC Submission

045054-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R6940 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

35928815-35937529 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

C to A at 35934118 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134221 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164502] [ENSMUST00000174521]

AlphaFold

F7C950

Predicted Effect

unknown
Transcript: ENSMUST00000164502
AA Change: A23S

SMART Domains

Protein: ENSMUSP00000130987
Gene: ENSMUSG00000090588
AA Change: A23S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	26	39	N/A	INTRINSIC
low complexity region	42	59	N/A	INTRINSIC
low complexity region	76	144	N/A	INTRINSIC
low complexity region	149	578	N/A	INTRINSIC
low complexity region	580	653	N/A	INTRINSIC
low complexity region	655	1179	N/A	INTRINSIC
low complexity region	1183	1373	N/A	INTRINSIC
low complexity region	1383	1436	N/A	INTRINSIC
low complexity region	1438	1479	N/A	INTRINSIC
Pfam:Epiglycanin_C	1518	1605	3.8e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.8%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg1	C	A	9: 54,516,381 (GRCm39)	A667S	probably benign	Het
Ank2	G	A	3: 126,735,621 (GRCm39)		probably benign	Het
Arhgef28	A	G	13: 98,102,038 (GRCm39)	L812P	possibly damaging	Het
Armc5	C	T	7: 127,839,470 (GRCm39)	R263*	probably null	Het
Cadps2	A	T	6: 23,302,491 (GRCm39)	V740E	probably damaging	Het
Cckbr	T	C	7: 105,084,103 (GRCm39)	V279A	probably benign	Het
Cd101	T	C	3: 100,911,018 (GRCm39)	D880G	probably damaging	Het
Cep350	A	T	1: 155,804,297 (GRCm39)	S929T	probably benign	Het
Chaf1b	G	A	16: 93,702,853 (GRCm39)	R556H	probably benign	Het
Csn1s1	A	T	5: 87,822,882 (GRCm39)	Q69L	possibly damaging	Het
Dennd1b	T	C	1: 138,981,155 (GRCm39)		probably null	Het
Dnah11	T	C	12: 118,162,503 (GRCm39)	D22G	probably benign	Het
Dnah7a	T	C	1: 53,670,836 (GRCm39)	H472R	probably benign	Het
Dnah7b	G	A	1: 46,158,428 (GRCm39)	R337H	probably benign	Het
Eif2ak3	T	C	6: 70,869,386 (GRCm39)	V691A	possibly damaging	Het
Ern2	T	C	7: 121,785,369 (GRCm39)	R3G	probably benign	Het
Fat3	T	C	9: 15,828,096 (GRCm39)		probably null	Het
Fnbp4	T	C	2: 90,575,858 (GRCm39)	F20L	unknown	Het
Gtf2b	C	A	3: 142,484,016 (GRCm39)	D63E	probably damaging	Het
Hydin	A	G	8: 111,217,243 (GRCm39)	Y1377C	probably damaging	Het
Klf1	A	G	8: 85,628,724 (GRCm39)	T41A	possibly damaging	Het
Lce3d	G	A	3: 92,865,541 (GRCm39)	S52N	unknown	Het
Lrit1	G	C	14: 36,782,052 (GRCm39)	V242L	probably damaging	Het
Lrrfip1	T	A	1: 91,050,135 (GRCm39)		probably null	Het
Ltbp4	A	C	7: 27,008,369 (GRCm39)	V1313G	probably damaging	Het
Morc1	C	A	16: 48,300,208 (GRCm39)	S235*	probably null	Het
Mr1	T	G	1: 155,005,014 (GRCm39)	*342S	probably null	Het
Nol4l	T	A	2: 153,253,684 (GRCm39)	S630C	probably benign	Het
Nr4a3	C	T	4: 48,051,486 (GRCm39)	P80L	probably benign	Het
Or51a43	A	G	7: 103,717,620 (GRCm39)	I206T	possibly damaging	Het
Otof	A	G	5: 30,528,987 (GRCm39)	V1807A	probably damaging	Het
Pde5a	T	A	3: 122,572,681 (GRCm39)	V354E	possibly damaging	Het
Pmfbp1	A	T	8: 110,251,823 (GRCm39)	E355D	probably damaging	Het
Ppp2r2c	A	G	5: 37,084,875 (GRCm39)	D112G	probably damaging	Het
Prkacb	A	T	3: 146,457,254 (GRCm39)	I37N	probably damaging	Het
Prox2	T	C	12: 85,141,348 (GRCm39)	D285G	probably benign	Het
Ranbp3l	A	G	15: 9,041,792 (GRCm39)	S61G	probably benign	Het
Rnf214	T	A	9: 45,802,196 (GRCm39)	T289S	probably damaging	Het
Rnf217	G	T	10: 31,381,973 (GRCm39)		probably null	Het
Sc5d	A	T	9: 42,166,723 (GRCm39)	M272K	probably benign	Het
Slco6c1	T	C	1: 97,000,626 (GRCm39)	T542A	possibly damaging	Het
Sord	T	A	2: 122,094,536 (GRCm39)	I308N	probably damaging	Het
Syvn1	A	T	19: 6,101,214 (GRCm39)		probably benign	Het
Tas2r134	A	G	2: 51,518,148 (GRCm39)	H209R	probably benign	Het
Trak1	T	C	9: 121,272,784 (GRCm39)	V214A	possibly damaging	Het
Trpm5	C	A	7: 142,638,547 (GRCm39)	E322*	probably null	Het
Ttf2	T	C	3: 100,876,831 (GRCm39)	T17A	probably damaging	Het
Vmn2r53	T	C	7: 12,316,343 (GRCm39)	E492G	probably benign	Het
Xndc1	T	C	7: 101,727,094 (GRCm39)	V161A	probably benign	Het

Other mutations in Muc21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
BB009:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
FR4304:Muc21	UTSW	17	35,933,013 (GRCm39)	intron	probably benign
R0334:Muc21	UTSW	17	35,933,614 (GRCm39)	intron	probably benign
R0946:Muc21	UTSW	17	35,929,105 (GRCm39)	missense	probably benign	0.32
R1117:Muc21	UTSW	17	35,930,920 (GRCm39)	intron	probably benign
R1345:Muc21	UTSW	17	35,932,489 (GRCm39)	intron	probably benign
R1697:Muc21	UTSW	17	35,931,540 (GRCm39)	intron	probably benign
R1750:Muc21	UTSW	17	35,931,940 (GRCm39)	intron	probably benign
R1756:Muc21	UTSW	17	35,930,131 (GRCm39)	intron	probably benign
R1946:Muc21	UTSW	17	35,933,416 (GRCm39)	intron	probably benign
R1978:Muc21	UTSW	17	35,933,857 (GRCm39)	intron	probably benign
R1991:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R1992:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R2063:Muc21	UTSW	17	35,932,297 (GRCm39)	intron	probably benign
R2356:Muc21	UTSW	17	35,932,563 (GRCm39)	intron	probably benign
R2866:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R3826:Muc21	UTSW	17	35,932,504 (GRCm39)	intron	probably benign
R4020:Muc21	UTSW	17	35,930,953 (GRCm39)	intron	probably benign
R4474:Muc21	UTSW	17	35,931,496 (GRCm39)	intron	probably benign
R4677:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R4786:Muc21	UTSW	17	35,930,221 (GRCm39)	intron	probably benign
R5071:Muc21	UTSW	17	35,931,444 (GRCm39)	intron	probably benign
R5173:Muc21	UTSW	17	35,931,633 (GRCm39)	intron	probably benign
R5283:Muc21	UTSW	17	35,932,224 (GRCm39)	intron	probably benign
R5446:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5542:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5716:Muc21	UTSW	17	35,931,675 (GRCm39)	intron	probably benign
R5913:Muc21	UTSW	17	35,934,123 (GRCm39)	intron	probably benign
R6011:Muc21	UTSW	17	35,933,074 (GRCm39)	intron	probably benign
R6198:Muc21	UTSW	17	35,931,808 (GRCm39)	intron	probably benign
R6394:Muc21	UTSW	17	35,931,058 (GRCm39)	intron	probably benign
R6786:Muc21	UTSW	17	35,934,057 (GRCm39)	intron	probably benign
R7082:Muc21	UTSW	17	35,932,093 (GRCm39)	missense	unknown
R7103:Muc21	UTSW	17	35,932,432 (GRCm39)	missense	unknown
R7110:Muc21	UTSW	17	35,933,510 (GRCm39)	intron	probably benign
R7139:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7165:Muc21	UTSW	17	35,932,870 (GRCm39)	missense	unknown
R7200:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7204:Muc21	UTSW	17	35,932,105 (GRCm39)	intron	probably benign
R7289:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7290:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7295:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7319:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R7462:Muc21	UTSW	17	35,931,568 (GRCm39)	missense	unknown
R7529:Muc21	UTSW	17	35,930,123 (GRCm39)	missense	unknown
R7718:Muc21	UTSW	17	35,933,728 (GRCm39)	missense	unknown
R7762:Muc21	UTSW	17	35,932,977 (GRCm39)	missense	unknown
R7788:Muc21	UTSW	17	35,929,798 (GRCm39)	missense	unknown
R7798:Muc21	UTSW	17	35,932,146 (GRCm39)	missense	unknown
R7831:Muc21	UTSW	17	35,929,651 (GRCm39)	missense	unknown
R7896:Muc21	UTSW	17	35,930,917 (GRCm39)	missense	unknown
R7899:Muc21	UTSW	17	35,931,493 (GRCm39)	intron	probably benign
R7932:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R8025:Muc21	UTSW	17	35,931,879 (GRCm39)	intron	probably benign
R8077:Muc21	UTSW	17	35,930,628 (GRCm39)	intron	probably benign
R8090:Muc21	UTSW	17	35,932,617 (GRCm39)	missense	unknown
R8169:Muc21	UTSW	17	35,932,072 (GRCm39)	missense	unknown
R8184:Muc21	UTSW	17	35,933,722 (GRCm39)	missense	unknown
R8209:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8226:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8464:Muc21	UTSW	17	35,933,098 (GRCm39)	intron	probably benign
R8670:Muc21	UTSW	17	35,932,540 (GRCm39)	missense	unknown
R8783:Muc21	UTSW	17	35,930,875 (GRCm39)	missense	unknown
R8856:Muc21	UTSW	17	35,931,865 (GRCm39)	missense	unknown
R9155:Muc21	UTSW	17	35,932,131 (GRCm39)	missense	unknown
R9214:Muc21	UTSW	17	35,931,838 (GRCm39)	missense	unknown
R9353:Muc21	UTSW	17	35,930,545 (GRCm39)	missense	unknown
R9618:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R9621:Muc21	UTSW	17	35,932,720 (GRCm39)	missense	unknown
R9679:Muc21	UTSW	17	35,930,491 (GRCm39)	missense	unknown
RF025:Muc21	UTSW	17	35,933,771 (GRCm39)	intron	probably benign
Z1176:Muc21	UTSW	17	35,932,137 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,951 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,817 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GTCAGGGTGGGCTTAGAGTC -3'
(R):5'- ATGTAGGCTAGGCTGGTCTAAAATT -3'

Sequencing Primer
(F):5'- GCTTAGAGTCTGAGGCAATGC -3'
(R):5'- AATTCCAGTGCCAGGGATTC -3'

Posted On

2018-11-06