Incidental Mutation 'R6947:Mettl25'
| ID |
540931 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mettl25
|
| Ensembl Gene |
ENSMUSG00000036009 |
| Gene Name |
methyltransferase like 25 |
| Synonyms |
BC067068 |
| MMRRC Submission |
045060-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.114)
|
| Stock # |
R6947 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
10 |
| Chromosomal Location |
105599050-105677241 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 105662053 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Phenylalanine to Leucine
at position 306
(F306L)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000038665
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000046638]
[ENSMUST00000176040]
[ENSMUST00000176924]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000046638
AA Change: F306L
PolyPhen 2
Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
|
| SMART Domains |
Protein: ENSMUSP00000038665
Gene: ENSMUSG00000036009
AA Change: F306L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Methyltransf_32
|
149 |
413 |
1.9e-30 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176040
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176924
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.8%
- 10x: 99.0%
- 20x: 96.0%
|
| Validation Efficiency |
100% (46/46) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 45 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adamts7 |
C |
A |
9: 90,073,857 (GRCm39) |
|
probably null |
Het |
| Adcy4 |
T |
C |
14: 56,015,848 (GRCm39) |
T414A |
possibly damaging |
Het |
| Bop1 |
A |
G |
15: 76,338,188 (GRCm39) |
F561L |
probably damaging |
Het |
| Ccdc18 |
T |
C |
5: 108,309,401 (GRCm39) |
V332A |
probably benign |
Het |
| Cep290 |
A |
G |
10: 100,365,918 (GRCm39) |
T1042A |
probably damaging |
Het |
| Cnot8 |
G |
T |
11: 58,008,331 (GRCm39) |
V266L |
probably benign |
Het |
| Cxxc4 |
C |
T |
3: 133,946,277 (GRCm39) |
S286F |
possibly damaging |
Het |
| Cyp2d12 |
A |
T |
15: 82,443,248 (GRCm39) |
I386L |
probably benign |
Het |
| Dgka |
A |
T |
10: 128,568,884 (GRCm39) |
I227N |
probably damaging |
Het |
| Eif2d |
T |
C |
1: 131,092,404 (GRCm39) |
V354A |
probably benign |
Het |
| Fgr |
T |
A |
4: 132,722,380 (GRCm39) |
|
probably null |
Het |
| Idnk |
A |
G |
13: 58,308,055 (GRCm39) |
|
probably null |
Het |
| Kmt2e |
T |
C |
5: 23,702,543 (GRCm39) |
S952P |
probably damaging |
Het |
| Kng1 |
A |
G |
16: 22,896,124 (GRCm39) |
H343R |
probably benign |
Het |
| Lgals8 |
G |
A |
13: 12,469,682 (GRCm39) |
|
probably benign |
Het |
| Lrit3 |
T |
A |
3: 129,582,883 (GRCm39) |
Q368L |
probably benign |
Het |
| Lypd8 |
A |
T |
11: 58,273,592 (GRCm39) |
T24S |
probably benign |
Het |
| Map3k4 |
G |
A |
17: 12,479,456 (GRCm39) |
Q704* |
probably null |
Het |
| Mcm3ap |
A |
G |
10: 76,351,500 (GRCm39) |
I1948V |
probably benign |
Het |
| Mos |
A |
C |
4: 3,871,585 (GRCm39) |
V77G |
probably damaging |
Het |
| Muc4 |
C |
T |
16: 32,596,177 (GRCm39) |
R3130C |
possibly damaging |
Het |
| Naxd |
T |
C |
8: 11,552,757 (GRCm39) |
V59A |
probably damaging |
Het |
| Nup107 |
A |
G |
10: 117,593,179 (GRCm39) |
V833A |
probably benign |
Het |
| Or1p4-ps1 |
C |
T |
11: 74,208,370 (GRCm39) |
S173L |
unknown |
Het |
| Or5m10b |
T |
C |
2: 85,699,271 (GRCm39) |
F112L |
probably benign |
Het |
| Pcnx2 |
C |
T |
8: 126,577,021 (GRCm39) |
|
probably null |
Het |
| Pde10a |
T |
A |
17: 9,188,424 (GRCm39) |
I908N |
probably damaging |
Het |
| Plcb1 |
A |
G |
2: 135,228,075 (GRCm39) |
K1058R |
probably benign |
Het |
| Rad17 |
A |
T |
13: 100,759,383 (GRCm39) |
F548Y |
probably damaging |
Het |
| Rad54b |
T |
G |
4: 11,569,859 (GRCm39) |
S58R |
possibly damaging |
Het |
| Rbm20 |
G |
A |
19: 53,839,696 (GRCm39) |
G895D |
probably damaging |
Het |
| Rgs22 |
T |
C |
15: 36,104,036 (GRCm39) |
|
probably null |
Het |
| Rhobtb3 |
A |
G |
13: 76,058,785 (GRCm39) |
S338P |
probably benign |
Het |
| Ruvbl2 |
A |
G |
7: 45,074,373 (GRCm39) |
|
probably null |
Het |
| Slc16a12 |
A |
G |
19: 34,650,007 (GRCm39) |
F343L |
probably benign |
Het |
| Slc22a28 |
A |
G |
19: 8,041,875 (GRCm39) |
L444P |
possibly damaging |
Het |
| Slc44a4 |
A |
T |
17: 35,147,044 (GRCm39) |
Q358L |
probably null |
Het |
| Sowaha |
A |
G |
11: 53,369,225 (GRCm39) |
F504L |
probably benign |
Het |
| Syne1 |
A |
G |
10: 5,125,789 (GRCm39) |
L6035P |
probably damaging |
Het |
| Thbs2 |
A |
T |
17: 14,910,029 (GRCm39) |
M190K |
possibly damaging |
Het |
| Tmem171 |
A |
G |
13: 98,824,950 (GRCm39) |
F227L |
possibly damaging |
Het |
| Trp53 |
A |
G |
11: 69,479,307 (GRCm39) |
K162E |
possibly damaging |
Het |
| Ttn |
T |
A |
2: 76,724,732 (GRCm39) |
K2098* |
probably null |
Het |
| Usp30 |
T |
C |
5: 114,241,821 (GRCm39) |
S88P |
probably benign |
Het |
| Zfp276 |
C |
A |
8: 123,981,643 (GRCm39) |
D63E |
probably benign |
Het |
|
| Other mutations in Mettl25 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00310:Mettl25
|
APN |
10 |
105,662,295 (GRCm39) |
missense |
probably benign |
|
|
IGL00698:Mettl25
|
APN |
10 |
105,629,201 (GRCm39) |
missense |
probably null |
0.31 |
|
IGL00766:Mettl25
|
APN |
10 |
105,615,443 (GRCm39) |
splice site |
probably benign |
|
|
IGL01360:Mettl25
|
APN |
10 |
105,659,058 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Mettl25
|
APN |
10 |
105,659,068 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02088:Mettl25
|
APN |
10 |
105,659,111 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02623:Mettl25
|
APN |
10 |
105,662,185 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL03245:Mettl25
|
APN |
10 |
105,662,358 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL03134:Mettl25
|
UTSW |
10 |
105,661,888 (GRCm39) |
nonsense |
probably null |
|
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1297:Mettl25
|
UTSW |
10 |
105,659,126 (GRCm39) |
missense |
probably benign |
0.11 |
|
R1482:Mettl25
|
UTSW |
10 |
105,662,451 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1526:Mettl25
|
UTSW |
10 |
105,668,844 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R1542:Mettl25
|
UTSW |
10 |
105,661,981 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1589:Mettl25
|
UTSW |
10 |
105,615,493 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1901:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1902:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R2016:Mettl25
|
UTSW |
10 |
105,633,167 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2355:Mettl25
|
UTSW |
10 |
105,599,316 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2920:Mettl25
|
UTSW |
10 |
105,601,038 (GRCm39) |
splice site |
probably null |
|
|
R3149:Mettl25
|
UTSW |
10 |
105,662,214 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4547:Mettl25
|
UTSW |
10 |
105,661,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4646:Mettl25
|
UTSW |
10 |
105,662,416 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4652:Mettl25
|
UTSW |
10 |
105,615,509 (GRCm39) |
nonsense |
probably null |
|
|
R5020:Mettl25
|
UTSW |
10 |
105,662,068 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R5435:Mettl25
|
UTSW |
10 |
105,615,447 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7075:Mettl25
|
UTSW |
10 |
105,665,785 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7729:Mettl25
|
UTSW |
10 |
105,601,871 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8074:Mettl25
|
UTSW |
10 |
105,661,941 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8108:Mettl25
|
UTSW |
10 |
105,659,040 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8415:Mettl25
|
UTSW |
10 |
105,662,002 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8937:Mettl25
|
UTSW |
10 |
105,601,122 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8949:Mettl25
|
UTSW |
10 |
105,668,714 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9497:Mettl25
|
UTSW |
10 |
105,677,090 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9772:Mettl25
|
UTSW |
10 |
105,633,127 (GRCm39) |
missense |
probably benign |
|
|
RF009:Mettl25
|
UTSW |
10 |
105,669,100 (GRCm39) |
intron |
probably benign |
|
|
Z1176:Mettl25
|
UTSW |
10 |
105,661,959 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- CCTCCAGATCTTCAATAATGTCATGC -3'
(R):5'- GCCCAGAGAGTTGAAAGTGC -3'
Sequencing Primer
(F):5'- GTCATGCAATTGTGAATCAGCAGTG -3'
(R):5'- CCCAGAGAGTTGAAAGTGCCAAAG -3'
|
| Posted On |
2018-11-28 |
Incidental Mutation