Mutagenetix > Incidental Mutation

Incidental Mutation 'R6966:Csnk2b'

542026

Institutional Source

Beutler Lab

Gene Symbol

Csnk2b

Ensembl Gene

ENSMUSG00000024387

Gene Name

casein kinase 2, beta polypeptide

Synonyms

MMRRC Submission

045076-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R6966 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

35335172-35341029 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 35336758 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 170 (I170L)

Ref Sequence

ENSEMBL: ENSMUSP00000134673 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025246] [ENSMUST00000062657] [ENSMUST00000165306] [ENSMUST00000172765] [ENSMUST00000172854] [ENSMUST00000173043] [ENSMUST00000173114] [ENSMUST00000174024] [ENSMUST00000173380] [ENSMUST00000173915] [ENSMUST00000174306] [ENSMUST00000174779]

AlphaFold

P67871

Predicted Effect

silent
Transcript: ENSMUST00000025246

SMART Domains

Protein: ENSMUSP00000025246
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	8	191	9.99e-143	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000062657

SMART Domains

Protein: ENSMUSP00000052133
Gene: ENSMUSG00000043807

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Blast:CK_II_beta	21	115	1e-40	BLAST
Blast:CK_II_beta	125	170	3e-6	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000165306

SMART Domains

Protein: ENSMUSP00000133240
Gene: ENSMUSG00000092417

Domain	Start	End	E-Value	Type
low complexity region	59	76	N/A	INTRINSIC
ANK	133	163	4.36e-1	SMART
G_patch	246	292	1.45e-14	SMART

Predicted Effect

silent
Transcript: ENSMUST00000172765

SMART Domains

Protein: ENSMUSP00000134523
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	29	206	6.86e-132	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000172854

SMART Domains

Protein: ENSMUSP00000138033
Gene: ENSMUSG00000043807

Domain	Start	End	E-Value	Type
Blast:CK_II_beta	1	60	2e-18	BLAST
Blast:CK_II_beta	70	115	1e-6	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000173043

SMART Domains

Protein: ENSMUSP00000135684
Gene: ENSMUSG00000092417

Domain	Start	End	E-Value	Type
low complexity region	59	76	N/A	INTRINSIC
ANK	133	163	2.7e-3	SMART

Predicted Effect

silent
Transcript: ENSMUST00000173114

SMART Domains

Protein: ENSMUSP00000134218
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	8	191	9.99e-143	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000174024
AA Change: I170L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000134673
Gene: ENSMUSG00000024387
AA Change: I170L

Domain	Start	End	E-Value	Type
CK_II_beta	8	169	5.07e-71	SMART
Blast:CK_II_beta	189	220	6e-7	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000173380

SMART Domains

Protein: ENSMUSP00000134694
Gene: ENSMUSG00000092417

Domain	Start	End	E-Value	Type
low complexity region	59	76	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000173633

SMART Domains

Protein: ENSMUSP00000133826
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	2	91	1.28e-26	SMART
Blast:CK_II_beta	121	154	4e-7	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000173915

SMART Domains

Protein: ENSMUSP00000133425
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	8	58	6.45e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000174306

SMART Domains

Protein: ENSMUSP00000134413
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	8	122	3.01e-64	SMART

Predicted Effect

silent
Transcript: ENSMUST00000174779

SMART Domains

Protein: ENSMUSP00000133684
Gene: ENSMUSG00000024387

Domain	Start	End	E-Value	Type
CK_II_beta	1	140	2.47e-83	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.2%
20x: 97.3%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes the beta subunit of the casein kinase 2 enzyme, which is a heterotetramer comprised of alpha and/or alpha-prime catalytic subunits and two regulatory beta subunits. Casein kinase 2 is involved in the regulation of several cellular processes including gene expression, protein synthesis and cell proliferation. Knockout of this gene in mice leads to embryonic lethality. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]
PHENOTYPE: Mice homozygous for a targeted mutation exhibit embryonic lethality post-implantation, growth retardation and arrest, a failure to form an inner cell mass and smaller size. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam26b	G	A	8: 43,974,472 (GRCm39)	R177C	possibly damaging	Het
Adgrb2	CG	C	4: 129,908,155 (GRCm39)		probably null	Het
Bahcc1	G	A	11: 120,173,985 (GRCm39)	V1582M	probably damaging	Het
Baiap2	A	T	11: 119,897,231 (GRCm39)	R529*	probably null	Het
Bcl9l	C	T	9: 44,420,685 (GRCm39)	Q1327*	probably null	Het
Brsk2	T	A	7: 141,538,270 (GRCm39)	C139S	possibly damaging	Het
Catsper3	T	A	13: 55,946,672 (GRCm39)	I123N	probably damaging	Het
Cd84	A	G	1: 171,713,976 (GRCm39)	N325D	possibly damaging	Het
Chid1	T	A	7: 141,076,297 (GRCm39)	Y357F	possibly damaging	Het
Clstn2	A	T	9: 97,408,459 (GRCm39)	Y416*	probably null	Het
Cnot1	A	T	8: 96,451,160 (GRCm39)	L2189Q	probably damaging	Het
Dnah3	T	C	7: 119,631,977 (GRCm39)	Q1326R	probably damaging	Het
Drd1	T	C	13: 54,207,564 (GRCm39)	I210V	probably damaging	Het
Eef1d	G	T	15: 75,775,558 (GRCm39)	Q34K	probably benign	Het
Fam184a	T	C	10: 53,531,095 (GRCm39)	T760A	probably benign	Het
Fnip1	G	T	11: 54,373,385 (GRCm39)	V199F	probably benign	Het
Glyatl3	T	C	17: 41,215,829 (GRCm39)	K226E	probably damaging	Het
Gm19410	C	T	8: 36,285,127 (GRCm39)	T2093I	possibly damaging	Het
Herc1	A	T	9: 66,318,347 (GRCm39)	E1206D	probably benign	Het
Hs6st1	G	A	1: 36,143,299 (GRCm39)	W411*	probably null	Het
Hsf2	C	A	10: 57,372,080 (GRCm39)	S60R	probably damaging	Het
Hyal5	C	A	6: 24,891,291 (GRCm39)	N368K	probably damaging	Het
Itgb2l	A	T	16: 96,231,843 (GRCm39)	F308I	probably benign	Het
Kdm7a	G	A	6: 39,129,773 (GRCm39)	L468F	probably damaging	Het
Lamp3	A	T	16: 19,518,403 (GRCm39)	L278*	probably null	Het
Ly6c1	A	T	15: 74,917,289 (GRCm39)		probably benign	Het
Mark3	A	G	12: 111,606,458 (GRCm39)	N524D	probably damaging	Het
Met	T	G	6: 17,531,531 (GRCm39)	L603R	possibly damaging	Het
Mical1	A	T	10: 41,355,750 (GRCm39)	Q198L	probably damaging	Het
Nacad	T	A	11: 6,552,634 (GRCm39)	I186F	possibly damaging	Het
Nt5c3b	A	T	11: 100,320,750 (GRCm39)	M257K	probably benign	Het
Nuak2	T	C	1: 132,252,770 (GRCm39)	M108T	possibly damaging	Het
Nub1	T	A	5: 24,894,470 (GRCm39)	Y51N	probably damaging	Het
Nxpe5	T	C	5: 138,237,679 (GRCm39)	S68P	probably damaging	Het
Or10ag58	C	T	2: 87,265,623 (GRCm39)	T264I	probably damaging	Het
Or5d44	A	G	2: 88,141,835 (GRCm39)	S102P	probably benign	Het
Or6c66	A	G	10: 129,461,633 (GRCm39)	V99A	probably benign	Het
Pias3	A	G	3: 96,609,511 (GRCm39)	D276G	probably damaging	Het
Setd7	A	G	3: 51,437,605 (GRCm39)	Y217H	probably damaging	Het
Slc23a1	A	T	18: 35,758,114 (GRCm39)	I142N	probably damaging	Het
Slx4ip	T	C	2: 136,910,144 (GRCm39)	S310P	probably damaging	Het
Tcp11l2	G	A	10: 84,427,133 (GRCm39)	R199Q	possibly damaging	Het
Tgm4	T	A	9: 122,880,207 (GRCm39)	D226E	possibly damaging	Het
Tspyl4	C	A	10: 34,173,673 (GRCm39)	A55E	probably benign	Het
Uap1l1	A	G	2: 25,254,950 (GRCm39)	I146T	probably damaging	Het
Ush2a	G	A	1: 188,308,441 (GRCm39)	G2030D	probably damaging	Het
Uts2r	G	A	11: 121,052,213 (GRCm39)	G359D	possibly damaging	Het
Vwa7	T	C	17: 35,236,072 (GRCm39)	S9P	probably benign	Het
Zbtb16	A	G	9: 48,568,654 (GRCm39)	C604R	probably damaging	Het
Zfp804b	A	G	5: 6,821,615 (GRCm39)	S483P	probably damaging	Het
Zfpm1	G	A	8: 123,058,904 (GRCm39)	A175T	probably damaging	Het

Other mutations in Csnk2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01979:Csnk2b	APN	17	35,336,992 (GRCm39)	missense	possibly damaging	0.92
R2318:Csnk2b	UTSW	17	35,337,037 (GRCm39)	missense	possibly damaging	0.88
R7053:Csnk2b	UTSW	17	35,335,422 (GRCm39)	intron	probably benign
R7693:Csnk2b	UTSW	17	35,336,972 (GRCm39)	missense	probably null	1.00
R7812:Csnk2b	UTSW	17	35,339,431 (GRCm39)	missense	probably benign	0.42
R7983:Csnk2b	UTSW	17	35,335,304 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- CTTTGGGATAGGCAAGCCTC -3'
(R):5'- ATCGGTGAGTGTTGAAAGCC -3'

Sequencing Primer
(F):5'- CAAGAAAGGGCTATGCTTGTCCTC -3'
(R):5'- AGAAGCTGCCTTCCTGTACTCAG -3'

Posted On

2018-11-28