Mutagenetix > Incidental Mutation

Incidental Mutation 'R6975:Gm6871'

542416

Institutional Source

Beutler Lab

Gene Symbol

Gm6871

Ensembl Gene

ENSMUSG00000090744

Gene Name

predicted gene 6871

Synonyms

MMRRC Submission

045085-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R6975 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41193704-41210253 bp(-) (GRCm39)

Type of Mutation

synonymous

DNA Base Change (assembly)

T to C at 41196202 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000105843 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073410] [ENSMUST00000110214] [ENSMUST00000164677]

AlphaFold

L7N248

Predicted Effect

silent
Transcript: ENSMUST00000073410

SMART Domains

Protein: ENSMUSP00000073117
Gene: ENSMUSG00000090744

Domain	Start	End	E-Value	Type
KRAB	4	64	1.19e-16	SMART
ZnF_C2H2	131	153	3.44e-4	SMART
ZnF_C2H2	159	181	5.99e-4	SMART
ZnF_C2H2	187	209	3.34e-2	SMART
ZnF_C2H2	215	237	5.99e-4	SMART
ZnF_C2H2	243	265	1.28e-3	SMART
ZnF_C2H2	271	293	3.69e-4	SMART
ZnF_C2H2	299	321	1.36e-2	SMART
ZnF_C2H2	327	349	3.21e-4	SMART
ZnF_C2H2	355	377	2.61e-4	SMART
ZnF_C2H2	383	405	3.16e-3	SMART
ZnF_C2H2	411	433	5.14e-3	SMART

Predicted Effect

silent
Transcript: ENSMUST00000110214

SMART Domains

Protein: ENSMUSP00000105843
Gene: ENSMUSG00000090744

Domain	Start	End	E-Value	Type
KRAB	111	171	1.19e-16	SMART
ZnF_C2H2	238	260	3.44e-4	SMART
ZnF_C2H2	266	288	5.99e-4	SMART
ZnF_C2H2	294	316	3.34e-2	SMART
ZnF_C2H2	322	344	5.99e-4	SMART
ZnF_C2H2	350	372	1.28e-3	SMART
ZnF_C2H2	378	400	3.69e-4	SMART
ZnF_C2H2	406	428	1.36e-2	SMART
ZnF_C2H2	434	456	3.21e-4	SMART
ZnF_C2H2	462	484	2.61e-4	SMART
ZnF_C2H2	490	512	3.16e-3	SMART
ZnF_C2H2	518	540	5.14e-3	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000131240
Gene: ENSMUSG00000090744
AA Change: E29G

Domain	Start	End	E-Value	Type
internal_repeat_1	21	67	6.62e-7	PROSPERO
internal_repeat_1	105	151	6.62e-7	PROSPERO

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.2%
20x: 96.9%

Validation Efficiency

100% (46/46)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010315B03Rik	T	A	9: 124,056,687 (GRCm39)	H100L	probably benign	Het
Apaf1	A	T	10: 90,856,596 (GRCm39)	H870Q	probably damaging	Het
Aplf	T	C	6: 87,623,068 (GRCm39)	D358G	probably damaging	Het
Atp10a	A	T	7: 58,423,733 (GRCm39)	S233C	probably damaging	Het
Ccdc158	A	T	5: 92,814,579 (GRCm39)	Y82*	probably null	Het
Clba1	C	T	12: 112,773,217 (GRCm39)	P70L	possibly damaging	Het
Cubn	T	C	2: 13,491,600 (GRCm39)	D149G	probably damaging	Het
Cyp4f14	T	C	17: 33,133,608 (GRCm39)	T83A	probably benign	Het
Endog	A	G	2: 30,061,648 (GRCm39)		probably benign	Het
Extl3	T	C	14: 65,304,246 (GRCm39)	E721G	probably benign	Het
Gatad1	T	C	5: 3,693,540 (GRCm39)	R210G	probably benign	Het
Gcn1	A	G	5: 115,751,518 (GRCm39)	H2033R	probably damaging	Het
Gm12830	T	C	4: 114,702,246 (GRCm39)	M136T		Het
Hoxc4	T	C	15: 102,944,098 (GRCm39)	S159P	probably damaging	Het
Ift70a2	T	C	2: 75,806,752 (GRCm39)	R587G	probably damaging	Het
Ift70a2	A	T	2: 75,808,004 (GRCm39)	Y169*	probably null	Het
Igf2bp2	T	A	16: 21,880,611 (GRCm39)	Q494L	probably null	Het
Igfn1	T	A	1: 135,896,183 (GRCm39)	N1461I	probably damaging	Het
Il23r	C	A	6: 67,400,352 (GRCm39)	K659N	probably damaging	Het
Map3k11	A	G	19: 5,740,755 (GRCm39)	S161G	possibly damaging	Het
Naip6	T	A	13: 100,452,773 (GRCm39)	Q96L	probably damaging	Het
Nup133	T	C	8: 124,642,057 (GRCm39)	E802G	probably damaging	Het
Nutm1	G	A	2: 112,086,563 (GRCm39)	S56F	probably damaging	Het
Or10x4	T	A	1: 174,219,243 (GRCm39)	S203T	probably benign	Het
Or51a24	T	C	7: 103,734,002 (GRCm39)	N95S	probably benign	Het
Otop2	A	T	11: 115,220,152 (GRCm39)	S331C	possibly damaging	Het
Pira1	C	G	7: 3,740,319 (GRCm39)	A301P	probably damaging	Het
Pkd1l3	A	G	8: 110,387,539 (GRCm39)	R1818G	possibly damaging	Het
Pms1	A	T	1: 53,228,590 (GRCm39)	I886N	probably damaging	Het
Pnpla6	A	G	8: 3,588,068 (GRCm39)	Y1107C	probably damaging	Het
Ppp3ca	A	G	3: 136,611,062 (GRCm39)	T362A	probably damaging	Het
Rarb	A	G	14: 16,574,942 (GRCm38)	S25P	possibly damaging	Het
Rnf121	A	T	7: 101,673,218 (GRCm39)		probably null	Het
Sae1	T	C	7: 16,070,712 (GRCm39)	Y266C	probably damaging	Het
Scpep1	A	T	11: 88,838,031 (GRCm39)	F85L	probably damaging	Het
Shank1	T	A	7: 43,962,530 (GRCm39)		probably null	Het
Slc40a1	T	C	1: 45,948,652 (GRCm39)	K543E	probably benign	Het
Slc9a9	A	G	9: 94,842,499 (GRCm39)	Y350C	probably damaging	Het
Sun2	G	T	15: 79,618,420 (GRCm39)	Y246*	probably null	Het
Tas1r2	T	A	4: 139,397,031 (GRCm39)	I790N	probably damaging	Het
Tmtc2	T	C	10: 105,158,863 (GRCm39)	T577A	probably benign	Het
Trim24	T	G	6: 37,896,427 (GRCm39)		probably null	Het
Ttc12	A	G	9: 49,349,718 (GRCm39)	V693A	probably benign	Het
Zbtb22	A	C	17: 34,136,938 (GRCm39)	D361A	probably damaging	Het
Zfp276	T	A	8: 123,983,570 (GRCm39)	C324*	probably null	Het
Zfp536	A	G	7: 37,267,952 (GRCm39)	L488P	probably damaging	Het

Other mutations in Gm6871

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00586:Gm6871	APN	7	41,195,845 (GRCm39)	missense	possibly damaging	0.67
R1005_Gm6871_627	UTSW	7	41,195,682 (GRCm39)	missense	probably damaging	1.00
R0419:Gm6871	UTSW	7	41,222,869 (GRCm39)	missense	probably benign	0.00
R1005:Gm6871	UTSW	7	41,195,682 (GRCm39)	missense	probably damaging	1.00
R1544:Gm6871	UTSW	7	41,195,514 (GRCm39)	splice site	probably null
R1553:Gm6871	UTSW	7	41,195,822 (GRCm39)	missense	probably benign	0.00
R1674:Gm6871	UTSW	7	41,223,059 (GRCm39)	missense	possibly damaging	0.46
R1710:Gm6871	UTSW	7	41,195,901 (GRCm39)	missense	probably damaging	1.00
R1743:Gm6871	UTSW	7	41,195,876 (GRCm39)	missense	probably damaging	0.98
R1777:Gm6871	UTSW	7	41,195,143 (GRCm39)	missense	probably benign	0.23
R1844:Gm6871	UTSW	7	41,222,892 (GRCm39)	missense	probably benign	0.03
R2508:Gm6871	UTSW	7	41,197,414 (GRCm39)	missense	probably benign	0.11
R2966:Gm6871	UTSW	7	41,222,864 (GRCm39)	missense	probably benign	0.07
R3155:Gm6871	UTSW	7	41,223,079 (GRCm39)	missense	probably benign	0.03
R3156:Gm6871	UTSW	7	41,223,079 (GRCm39)	missense	probably benign	0.03
R3967:Gm6871	UTSW	7	41,196,148 (GRCm39)	missense	probably damaging	0.99
R4156:Gm6871	UTSW	7	41,195,510 (GRCm39)	missense	probably damaging	0.96
R4238:Gm6871	UTSW	7	41,195,204 (GRCm39)	missense	probably damaging	1.00
R4239:Gm6871	UTSW	7	41,195,204 (GRCm39)	missense	probably damaging	1.00
R4240:Gm6871	UTSW	7	41,195,204 (GRCm39)	missense	probably damaging	1.00
R4731:Gm6871	UTSW	7	41,196,173 (GRCm39)	missense	probably benign	0.01
R4732:Gm6871	UTSW	7	41,196,173 (GRCm39)	missense	probably benign	0.01
R4733:Gm6871	UTSW	7	41,196,173 (GRCm39)	missense	probably benign	0.01
R4910:Gm6871	UTSW	7	41,223,016 (GRCm39)	missense	probably benign	0.03
R5269:Gm6871	UTSW	7	41,197,525 (GRCm39)	missense	probably damaging	0.99
R5371:Gm6871	UTSW	7	41,222,992 (GRCm39)	missense	probably benign	0.07
R6222:Gm6871	UTSW	7	41,196,006 (GRCm39)	missense	probably damaging	0.99
R8006:Gm6871	UTSW	7	41,195,106 (GRCm39)	missense	probably benign	0.10
R8150:Gm6871	UTSW	7	41,197,185 (GRCm39)	missense
R9019:Gm6871	UTSW	7	41,195,262 (GRCm39)	missense	probably damaging	1.00
Z1176:Gm6871	UTSW	7	41,195,837 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CTCTCCAGTATGTGTTCTTTTATGCA -3'
(R):5'- GGGAACACCAAGACTGTGAACA -3'

Sequencing Primer
(F):5'- GTGATGTGCAAAGGCTTTACCAC -3'
(R):5'- CACCAAGACTGTGAACAATGTG -3'

Posted On

2018-11-28