Incidental Mutation 'R6986:Ehd4'
ID542949
Institutional Source Beutler Lab
Gene Symbol Ehd4
Ensembl Gene ENSMUSG00000027293
Gene NameEH-domain containing 4
Synonyms2210022F10Rik
MMRRC Submission
Accession Numbers
Is this an essential gene? Possibly essential (E-score: 0.586) question?
Stock #R6986 (G1)
Quality Score225.009
Status Not validated
Chromosome2
Chromosomal Location120089175-120154606 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 120097090 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Lysine at position 321 (M321K)
Ref Sequence ENSEMBL: ENSMUSP00000028755 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000028755]
Predicted Effect probably damaging
Transcript: ENSMUST00000028755
AA Change: M321K

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000028755
Gene: ENSMUSG00000027293
AA Change: M321K

DomainStartEndE-ValueType
Pfam:EHD_N 27 59 3e-20 PFAM
Pfam:MMR_HSR1 63 223 6.5e-7 PFAM
Pfam:Dynamin_N 64 224 3.9e-14 PFAM
EH 441 534 1.55e-39 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.3%
  • 20x: 98.1%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced male fertility. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adcy9 T C 16: 4,311,577 N482D probably damaging Het
Agbl3 A G 6: 34,839,452 K766E probably benign Het
Aldh3a1 T A 11: 61,214,251 I154N probably damaging Het
Cdca2 C T 14: 67,694,997 V486I probably benign Het
Celf3 G C 3: 94,487,717 A313P possibly damaging Het
Cgrrf1 T C 14: 46,832,129 probably benign Het
Chd7 T A 4: 8,859,285 N2458K possibly damaging Het
Chrna5 T A 9: 55,006,457 M429K possibly damaging Het
Cux2 A T 5: 121,868,579 L876Q possibly damaging Het
Cyb5rl T A 4: 107,070,876 S3T probably benign Het
Ell2 C A 13: 75,761,887 L119M probably damaging Het
Endod1 T C 9: 14,357,310 E293G probably damaging Het
Fam205c A G 4: 42,868,696 V309A possibly damaging Het
Fat3 G A 9: 16,021,335 T1434I probably damaging Het
Flrt2 T A 12: 95,780,685 I599N probably damaging Het
Gm6370 T A 5: 146,493,588 probably null Het
Gm7247 T C 14: 51,365,375 M56T possibly damaging Het
Herc2 G A 7: 56,106,453 R747H possibly damaging Het
Hist1h3g A G 13: 23,535,433 probably benign Het
Ints8 T C 4: 11,204,474 Y994C probably damaging Het
Irf1 A G 11: 53,774,140 T165A probably damaging Het
Lgr6 G T 1: 134,993,956 Q217K possibly damaging Het
Lrrc36 T C 8: 105,458,447 S619P probably damaging Het
Marc2 T C 1: 184,841,263 H53R probably benign Het
Morn4 T C 19: 42,078,014 N45D possibly damaging Het
Mtmr3 T C 11: 4,489,692 T583A probably damaging Het
Ncdn A G 4: 126,747,229 Y493H probably damaging Het
Nckap1 T A 2: 80,520,567 Y726F probably benign Het
Olfr225 A G 11: 59,614,098 K378R possibly damaging Het
Olfr822 G A 10: 130,075,330 V307M possibly damaging Het
Optc T A 1: 133,897,964 M275L probably benign Het
Perm1 A T 4: 156,218,519 K507* probably null Het
Pgbd5 C T 8: 124,384,473 E160K possibly damaging Het
Polr2k C A 15: 36,175,035 P14T probably benign Het
Ppp2r3d A T 9: 124,439,080 Y112* probably null Het
Pramef17 T A 4: 143,993,318 D159V probably damaging Het
Rnase9 T C 14: 51,039,080 N147S probably benign Het
Rnls C T 19: 33,382,381 G11D probably damaging Het
Sbf2 T C 7: 110,330,615 T333A probably damaging Het
Scn3a A T 2: 65,508,618 N572K probably damaging Het
Serinc3 A G 2: 163,627,971 L319P probably benign Het
Setbp1 T C 18: 78,857,839 D871G probably damaging Het
Sf3b2 T C 19: 5,279,895 T661A probably benign Het
Sgsm2 T A 11: 74,892,041 R30W probably damaging Het
Sh3tc1 A G 5: 35,723,944 S52P probably benign Het
Smox C T 2: 131,522,111 A45V possibly damaging Het
Snrpa A T 7: 27,192,964 M57K probably damaging Het
Srbd1 A T 17: 85,985,222 I973N probably damaging Het
Srp72 T A 5: 76,994,876 S428T probably benign Het
Ss18l2 A G 9: 121,712,605 Y50C probably damaging Het
St14 A G 9: 31,096,549 L578P probably damaging Het
Tcrg-V6 G T 13: 19,190,644 G40W possibly damaging Het
Ticam1 C T 17: 56,269,900 E732K probably benign Het
Tmc1 T C 19: 20,824,283 N460D probably benign Het
Tnni3k C T 3: 154,961,864 G280R probably damaging Het
Trpc3 A G 3: 36,655,016 probably null Het
Tshr C T 12: 91,533,957 A152V probably damaging Het
Tuft1 G A 3: 94,614,154 P358S probably damaging Het
Vmn2r120 A T 17: 57,509,340 S672T probably damaging Het
Vmn2r83 G T 10: 79,480,259 E496D probably benign Het
Vmn2r91 A G 17: 18,136,009 N646S probably benign Het
Zfp9 A G 6: 118,464,465 V412A possibly damaging Het
Zmynd8 G T 2: 165,833,415 H273N probably damaging Het
Other mutations in Ehd4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00514:Ehd4 APN 2 120091213 missense probably damaging 1.00
IGL00673:Ehd4 APN 2 120102220 missense probably damaging 1.00
IGL00919:Ehd4 APN 2 120097054 missense possibly damaging 0.75
IGL00964:Ehd4 APN 2 120127682 missense probably benign 0.38
IGL01801:Ehd4 APN 2 120102341 missense probably damaging 1.00
R0436:Ehd4 UTSW 2 120102341 missense probably damaging 1.00
R1398:Ehd4 UTSW 2 120127600 missense probably benign 0.07
R1818:Ehd4 UTSW 2 120102404 nonsense probably null
R1927:Ehd4 UTSW 2 120091492 missense probably benign 0.12
R3870:Ehd4 UTSW 2 120136953 missense probably damaging 1.00
R4178:Ehd4 UTSW 2 120154348 missense probably damaging 1.00
R5551:Ehd4 UTSW 2 120127619 missense possibly damaging 0.93
R6153:Ehd4 UTSW 2 120102423 missense probably damaging 1.00
R6164:Ehd4 UTSW 2 120102208 missense possibly damaging 0.70
R6172:Ehd4 UTSW 2 120102256 nonsense probably null
R6684:Ehd4 UTSW 2 120154334 missense probably damaging 1.00
R7282:Ehd4 UTSW 2 120091248 missense probably damaging 1.00
R7354:Ehd4 UTSW 2 120102132 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TGGGCCTGTGCAGATGTAAC -3'
(R):5'- AACTCTGGAAGTGCATTCTAGC -3'

Sequencing Primer
(F):5'- CCTGTGCAGATGTAACCAGCTAG -3'
(R):5'- GAAGTGCATTCTAGCTGCCCTTAG -3'
Posted On2018-11-28