Incidental Mutation 'R6986:Olfr225'
ID542985
Institutional Source Beutler Lab
Gene Symbol Olfr225
Ensembl Gene ENSMUSG00000044061
Gene Nameolfactory receptor 225
SynonymsGA_x6K02T0073K-490-1545, MOR256-51
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.059) question?
Stock #R6986 (G1)
Quality Score225.009
Status Not validated
Chromosome11
Chromosomal Location59612966-59613882 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 59614098 bp
ZygosityHeterozygous
Amino Acid Change Lysine to Arginine at position 378 (K378R)
Ref Sequence ENSEMBL: ENSMUSP00000051482 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000055276] [ENSMUST00000213169]
Predicted Effect possibly damaging
Transcript: ENSMUST00000055276
AA Change: K378R

PolyPhen 2 Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000051482
Gene: ENSMUSG00000044061
AA Change: K378R

DomainStartEndE-ValueType
Pfam:7tm_4 29 307 6.6e-47 PFAM
Pfam:7tm_1 40 289 4.3e-22 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000213169
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.3%
  • 20x: 98.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adcy9 T C 16: 4,311,577 N482D probably damaging Het
Agbl3 A G 6: 34,839,452 K766E probably benign Het
Aldh3a1 T A 11: 61,214,251 I154N probably damaging Het
Cdca2 C T 14: 67,694,997 V486I probably benign Het
Celf3 G C 3: 94,487,717 A313P possibly damaging Het
Cgrrf1 T C 14: 46,832,129 probably benign Het
Chd7 T A 4: 8,859,285 N2458K possibly damaging Het
Chrna5 T A 9: 55,006,457 M429K possibly damaging Het
Cux2 A T 5: 121,868,579 L876Q possibly damaging Het
Cyb5rl T A 4: 107,070,876 S3T probably benign Het
Ehd4 A T 2: 120,097,090 M321K probably damaging Het
Ell2 C A 13: 75,761,887 L119M probably damaging Het
Endod1 T C 9: 14,357,310 E293G probably damaging Het
Fam205c A G 4: 42,868,696 V309A possibly damaging Het
Fat3 G A 9: 16,021,335 T1434I probably damaging Het
Flrt2 T A 12: 95,780,685 I599N probably damaging Het
Gm6370 T A 5: 146,493,588 probably null Het
Gm7247 T C 14: 51,365,375 M56T possibly damaging Het
Herc2 G A 7: 56,106,453 R747H possibly damaging Het
Hist1h3g A G 13: 23,535,433 probably benign Het
Ints8 T C 4: 11,204,474 Y994C probably damaging Het
Irf1 A G 11: 53,774,140 T165A probably damaging Het
Lgr6 G T 1: 134,993,956 Q217K possibly damaging Het
Lrrc36 T C 8: 105,458,447 S619P probably damaging Het
Marc2 T C 1: 184,841,263 H53R probably benign Het
Morn4 T C 19: 42,078,014 N45D possibly damaging Het
Mtmr3 T C 11: 4,489,692 T583A probably damaging Het
Ncdn A G 4: 126,747,229 Y493H probably damaging Het
Nckap1 T A 2: 80,520,567 Y726F probably benign Het
Olfr822 G A 10: 130,075,330 V307M possibly damaging Het
Optc T A 1: 133,897,964 M275L probably benign Het
Perm1 A T 4: 156,218,519 K507* probably null Het
Pgbd5 C T 8: 124,384,473 E160K possibly damaging Het
Polr2k C A 15: 36,175,035 P14T probably benign Het
Ppp2r3d A T 9: 124,439,080 Y112* probably null Het
Pramef17 T A 4: 143,993,318 D159V probably damaging Het
Rnase9 T C 14: 51,039,080 N147S probably benign Het
Rnls C T 19: 33,382,381 G11D probably damaging Het
Sbf2 T C 7: 110,330,615 T333A probably damaging Het
Scn3a A T 2: 65,508,618 N572K probably damaging Het
Serinc3 A G 2: 163,627,971 L319P probably benign Het
Setbp1 T C 18: 78,857,839 D871G probably damaging Het
Sf3b2 T C 19: 5,279,895 T661A probably benign Het
Sgsm2 T A 11: 74,892,041 R30W probably damaging Het
Sh3tc1 A G 5: 35,723,944 S52P probably benign Het
Smox C T 2: 131,522,111 A45V possibly damaging Het
Snrpa A T 7: 27,192,964 M57K probably damaging Het
Srbd1 A T 17: 85,985,222 I973N probably damaging Het
Srp72 T A 5: 76,994,876 S428T probably benign Het
Ss18l2 A G 9: 121,712,605 Y50C probably damaging Het
St14 A G 9: 31,096,549 L578P probably damaging Het
Tcrg-V6 G T 13: 19,190,644 G40W possibly damaging Het
Ticam1 C T 17: 56,269,900 E732K probably benign Het
Tmc1 T C 19: 20,824,283 N460D probably benign Het
Tnni3k C T 3: 154,961,864 G280R probably damaging Het
Trpc3 A G 3: 36,655,016 probably null Het
Tshr C T 12: 91,533,957 A152V probably damaging Het
Tuft1 G A 3: 94,614,154 P358S probably damaging Het
Vmn2r120 A T 17: 57,509,340 S672T probably damaging Het
Vmn2r83 G T 10: 79,480,259 E496D probably benign Het
Vmn2r91 A G 17: 18,136,009 N646S probably benign Het
Zfp9 A G 6: 118,464,465 V412A possibly damaging Het
Zmynd8 G T 2: 165,833,415 H273N probably damaging Het
Other mutations in Olfr225
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00090:Olfr225 APN 11 59613321 missense possibly damaging 0.86
IGL01835:Olfr225 APN 11 59613339 missense probably damaging 0.99
IGL02006:Olfr225 APN 11 59613159 missense probably damaging 1.00
IGL03195:Olfr225 APN 11 59613803 missense probably damaging 1.00
R0148:Olfr225 UTSW 11 59613494 missense probably damaging 1.00
R0831:Olfr225 UTSW 11 59613654 missense possibly damaging 0.70
R1924:Olfr225 UTSW 11 59613123 missense possibly damaging 0.58
R2184:Olfr225 UTSW 11 59613138 missense probably damaging 1.00
R5772:Olfr225 UTSW 11 59613886 missense probably benign
R7405:Olfr225 UTSW 11 59614073 missense possibly damaging 0.53
Predicted Primers PCR Primer
(F):5'- ATAGAAACCTGCTCGACCCG -3'
(R):5'- CTATGGGAGAGAGCAAGTGTTC -3'

Sequencing Primer
(F):5'- GCACTGACAGGACCTGGAACTC -3'
(R):5'- GAGAGAGCAAGTGTTCCCATTACTTC -3'
Posted On2018-11-28