|Institutional Source||Beutler Lab|
|Gene Name||interleukin 12 receptor, beta 1|
|Is this an essential gene?||Probably non essential (E-score: 0.150)|
|Stock #||R6484 (G1)|
|Chromosomal Location||70808449-70821424 bp(+) (GRCm38)|
|Type of Mutation||unclassified (98 bp from exon)|
|DNA Base Change (assembly)||C to T at 70809704 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000148314 (fasta)|
|Predicted Effect||probably null
|Coding Region Coverage||
|Validation Efficiency||100% (60/60)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased serum IFN-gamma levels in response to recombinant IL-12 or LPS treatment, and failure of ConA-activated splenocytes to proliferate or secrete IFN-gamma in response to IL-12. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Il12rb1||
(F):5'- GCAGCTTGCTTTTCTAGGGC -3'
(R):5'- AACGATGTCTTCTCAACGCAGC -3'
(F):5'- CAGCTTGCTTTTCTAGGGCAAATG -3'
(R):5'- GCAGCAGCCATCACCTG -3'