Mutagenetix > Incidental Mutation

Incidental Mutation 'R6769:Gm7361'

543624

Institutional Source

Beutler Lab

Gene Symbol

Gm7361

Ensembl Gene

ENSMUSG00000059645

Gene Name

predicted gene 7361

Synonyms

MMRRC Submission

044885-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R6769 (G1)

Quality Score

40.0075

Status

Validated

Chromosome

Chromosomal Location

26462689-26469306 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 26462767 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 3 (L3*)

Ref Sequence

ENSEMBL: ENSMUSP00000073781 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074148]

AlphaFold

D3Z6R1

Predicted Effect

probably null
Transcript: ENSMUST00000074148
AA Change: L3*

SMART Domains

Protein: ENSMUSP00000073781
Gene: ENSMUSG00000059645
AA Change: L3*

Domain	Start	End	E-Value	Type
Pfam:Takusan	50	134	2.1e-26	PFAM
low complexity region	154	168	N/A	INTRINSIC
low complexity region	235	259	N/A	INTRINSIC

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 95.9%

Validation Efficiency

98% (39/40)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd8	G	T	8: 71,914,105 (GRCm39)	H174Q	probably damaging	Het
Alox5	C	A	6: 116,392,145 (GRCm39)		probably null	Het
Amer3	C	A	1: 34,627,330 (GRCm39)	A523D	possibly damaging	Het
Apoa4	G	A	9: 46,154,465 (GRCm39)	M355I	probably benign	Het
Atp10b	T	C	11: 43,094,079 (GRCm39)		probably null	Het
Atp9a	A	T	2: 168,516,820 (GRCm39)	Y340N	probably damaging	Het
C1rb	T	G	6: 124,554,364 (GRCm39)	S382A	probably benign	Het
Csmd1	A	G	8: 16,121,408 (GRCm39)	V1763A	possibly damaging	Het
Ctnnd1	C	A	2: 84,450,269 (GRCm39)	R317L	probably damaging	Het
Ctnnd1	A	T	2: 84,450,454 (GRCm39)	D255E	probably damaging	Het
Dipk1b	A	G	2: 26,524,875 (GRCm39)	R92G	probably benign	Het
Dmxl2	A	T	9: 54,323,808 (GRCm39)	I1192N	probably damaging	Het
Efcab5	A	G	11: 76,996,258 (GRCm39)	V1101A	probably damaging	Het
Eml2	G	A	7: 18,935,088 (GRCm39)	V432I	probably damaging	Het
Flg	T	C	3: 93,195,630 (GRCm39)		probably benign	Het
Fras1	A	G	5: 96,746,800 (GRCm39)	I623M	possibly damaging	Het
Gpr35	T	A	1: 92,910,426 (GRCm39)	M46K	probably damaging	Het
Ighv2-9	A	T	12: 113,842,930 (GRCm39)	F46I	possibly damaging	Het
Kdm4b	C	T	17: 56,658,754 (GRCm39)	A33V	possibly damaging	Het
Klhl5	T	A	5: 65,321,995 (GRCm39)	Y534N	probably damaging	Het
Mccc1	C	T	3: 36,043,992 (GRCm39)		probably null	Het
Memo1	A	T	17: 74,508,273 (GRCm39)	F270L	probably damaging	Het
Mnat1	A	G	12: 73,319,196 (GRCm39)	N273S	probably benign	Het
Niban2	C	T	2: 32,785,666 (GRCm39)			Het
Nxpe3	C	A	16: 55,686,471 (GRCm39)	G179V	probably damaging	Het
Or5b120	T	A	19: 13,480,318 (GRCm39)	F204I	possibly damaging	Het
Otp	A	G	13: 95,012,294 (GRCm39)	D6G	probably damaging	Het
Prdm5	T	A	6: 65,839,920 (GRCm39)	I265K	probably damaging	Het
Ptpn4	T	A	1: 119,643,698 (GRCm39)	N350I	probably benign	Het
Rassf10	A	G	7: 112,553,635 (GRCm39)	M79V	probably benign	Het
Rassf4	T	A	6: 116,618,433 (GRCm39)	D215V	possibly damaging	Het
Rbm12	A	T	2: 155,939,375 (GRCm39)	I299N	possibly damaging	Het
Scfd2	T	C	5: 74,692,117 (GRCm39)	H55R	probably benign	Het
Scn8a	A	G	15: 100,933,445 (GRCm39)	N1550D	probably benign	Het
Skic2	G	A	17: 35,064,166 (GRCm39)	R507*	probably null	Het
Slc30a5	A	C	13: 100,950,368 (GRCm39)	S240A	probably benign	Het
Slc7a4	A	G	16: 17,393,184 (GRCm39)	V205A	possibly damaging	Het
Syde2	G	A	3: 145,704,803 (GRCm39)	G318E	probably damaging	Het
Ttn	T	A	2: 76,538,883 (GRCm39)	D34649V	possibly damaging	Het
Vmn2r83	T	A	10: 79,313,856 (GRCm39)	S155T	probably damaging	Het

Other mutations in Gm7361

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02447:Gm7361	APN	5	26,462,853 (GRCm39)	missense	probably benign
IGL02550:Gm7361	APN	5	26,466,120 (GRCm39)	missense	possibly damaging	0.79
R0011:Gm7361	UTSW	5	26,463,876 (GRCm39)	splice site	probably benign
R0959:Gm7361	UTSW	5	26,467,051 (GRCm39)	missense	possibly damaging	0.86
R1246:Gm7361	UTSW	5	26,466,225 (GRCm39)	nonsense	probably null
R1580:Gm7361	UTSW	5	26,462,768 (GRCm39)	missense	probably damaging	0.97
R1654:Gm7361	UTSW	5	26,466,097 (GRCm39)	missense	probably damaging	0.99
R2065:Gm7361	UTSW	5	26,467,149 (GRCm39)	missense	probably damaging	1.00
R4864:Gm7361	UTSW	5	26,467,008 (GRCm39)	critical splice acceptor site	probably null
R5386:Gm7361	UTSW	5	26,463,903 (GRCm39)	missense	probably benign
R6282:Gm7361	UTSW	5	26,465,411 (GRCm39)	missense	probably benign	0.13
R6391:Gm7361	UTSW	5	26,463,960 (GRCm39)	missense	probably benign	0.37
R7205:Gm7361	UTSW	5	26,466,116 (GRCm39)	missense	probably damaging	0.99
R7497:Gm7361	UTSW	5	26,466,188 (GRCm39)	missense	probably benign
R7498:Gm7361	UTSW	5	26,466,188 (GRCm39)	missense	probably benign
R7499:Gm7361	UTSW	5	26,466,188 (GRCm39)	missense	probably benign
R8086:Gm7361	UTSW	5	26,465,446 (GRCm39)	missense	probably damaging	0.99
R8325:Gm7361	UTSW	5	26,467,154 (GRCm39)	missense	probably damaging	0.98
R8349:Gm7361	UTSW	5	26,465,387 (GRCm39)	missense	possibly damaging	0.95
R8449:Gm7361	UTSW	5	26,465,387 (GRCm39)	missense	possibly damaging	0.95
Z1177:Gm7361	UTSW	5	26,466,186 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AAAGGGCAGCTCAGCTTACTG -3'
(R):5'- TCCACATTACTGGAAAGGCC -3'

Sequencing Primer
(F):5'- AGCTCAGCTTACTGTCAGTACAGG -3'
(R):5'- AGGCCAGCTCAGGTAACC -3'

Posted On

2019-04-04