Mutagenetix > Incidental Mutation

Incidental Mutation 'R7003:Vmn1r225'

544694

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r225

Ensembl Gene

ENSMUSG00000043537

Gene Name

vomeronasal 1 receptor 225

Synonyms

V1re5

MMRRC Submission

045108-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R7003 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20722561-20723457 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20723416 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 286 (M286V)

Ref Sequence

ENSEMBL: ENSMUSP00000056068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061660]

AlphaFold

Q8R2A5

Predicted Effect

probably null
Transcript: ENSMUST00000061660
AA Change: M286V

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000056068
Gene: ENSMUSG00000043537
AA Change: M286V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	287	8.9e-15	PFAM
Pfam:V1R	11	291	3.1e-24	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actbl2	T	A	13: 111,392,490 (GRCm39)	I275N	probably damaging	Het
Actr3b	A	C	5: 26,003,461 (GRCm39)	Y21S	probably damaging	Het
Adam6b	C	T	12: 113,453,662 (GRCm39)	Q160*	probably null	Het
Adgrv1	C	T	13: 81,670,223 (GRCm39)		probably null	Het
Akr1c6	A	G	13: 4,504,514 (GRCm39)	N300D	probably benign	Het
Alox8	T	C	11: 69,082,416 (GRCm39)	D170G	possibly damaging	Het
Amhr2	A	G	15: 102,354,768 (GRCm39)	N40S	probably benign	Het
Ap2a2	A	G	7: 141,209,109 (GRCm39)	N767S	probably benign	Het
Armc3	T	C	2: 19,274,839 (GRCm39)	I358T	probably damaging	Het
Atg2b	A	G	12: 105,620,508 (GRCm39)	S732P	probably benign	Het
Atp12a	A	G	14: 56,610,837 (GRCm39)	Y327C	possibly damaging	Het
Bcr	T	C	10: 74,897,393 (GRCm39)	V179A	probably benign	Het
Cep104	T	C	4: 154,078,018 (GRCm39)	L642P	probably benign	Het
Clspn	T	A	4: 126,486,513 (GRCm39)	S1302R	possibly damaging	Het
Cmip	T	C	8: 118,111,727 (GRCm39)	F153L	probably benign	Het
Cplane1	T	G	15: 8,258,246 (GRCm39)	L2164R	probably damaging	Het
D630039A03Rik	T	C	4: 57,910,521 (GRCm39)	D97G	probably damaging	Het
Dok7	A	T	5: 35,236,899 (GRCm39)	T396S	probably benign	Het
Dsel	C	T	1: 111,788,025 (GRCm39)	V837I	probably benign	Het
Etl4	C	T	2: 20,810,695 (GRCm39)	T926I	probably benign	Het
Gpatch2l	G	A	12: 86,290,958 (GRCm39)	R47H	probably damaging	Het
Gpr155	A	G	2: 73,173,961 (GRCm39)	I816T	probably damaging	Het
Hpn	C	T	7: 30,810,367 (GRCm39)		probably benign	Het
Inpp5e	A	G	2: 26,287,877 (GRCm39)	S640P	probably benign	Het
Irs3	C	T	5: 137,643,539 (GRCm39)	V82I	probably benign	Het
Kif16b	A	C	2: 142,600,749 (GRCm39)	D461E	possibly damaging	Het
Krba1	A	G	6: 48,390,014 (GRCm39)	T592A	possibly damaging	Het
Lgsn	T	A	1: 31,243,024 (GRCm39)	S369T	possibly damaging	Het
Lrrc4b	C	T	7: 44,094,580 (GRCm39)	P83S	probably damaging	Het
Mplkipl1	A	G	19: 61,164,319 (GRCm39)	S39P	possibly damaging	Het
Neil3	T	C	8: 54,054,001 (GRCm39)	T343A	possibly damaging	Het
Nt5e	A	G	9: 88,246,805 (GRCm39)	Y347C	probably damaging	Het
Or1j1	A	G	2: 36,703,047 (GRCm39)	I19T	possibly damaging	Het
Or2d3b	A	T	7: 106,514,319 (GRCm39)	T305S	probably benign	Het
Or2f2	C	T	6: 42,767,399 (GRCm39)	T142I	probably benign	Het
Or8g26	A	T	9: 39,096,239 (GRCm39)	Y255F	probably benign	Het
P2rx5	T	C	11: 73,058,800 (GRCm39)		probably null	Het
Phtf2	A	G	5: 20,999,399 (GRCm39)	V248A	probably benign	Het
Plekhd1	T	C	12: 80,768,734 (GRCm39)	C406R	possibly damaging	Het
Plod3	T	A	5: 137,018,498 (GRCm39)	N245K	probably damaging	Het
Polr3c	G	T	3: 96,630,954 (GRCm39)	H155Q	possibly damaging	Het
Psap	T	A	10: 60,135,276 (GRCm39)	C317S	probably damaging	Het
Rif1	A	G	2: 51,967,001 (GRCm39)	I97V	probably benign	Het
Rnf123	T	A	9: 107,940,882 (GRCm39)		probably null	Het
Rnf19a	G	A	15: 36,254,650 (GRCm39)	R303*	probably null	Het
Sdk1	G	A	5: 142,082,489 (GRCm39)	V1036I	probably benign	Het
Shc3	T	A	13: 51,620,588 (GRCm39)	Y146F	probably benign	Het
Skint6	A	G	4: 112,963,109 (GRCm39)	Y441H	probably benign	Het
Slc7a12	T	C	3: 14,570,580 (GRCm39)	I173T	probably damaging	Het
Spesp1	A	T	9: 62,189,302 (GRCm39)	S15T	possibly damaging	Het
Tarm1	A	T	7: 3,545,939 (GRCm39)		probably null	Het
Tenm3	T	C	8: 48,693,479 (GRCm39)	Y1817C	probably damaging	Het
Ttc9c	A	T	19: 8,795,904 (GRCm39)	L45Q	probably damaging	Het
Ube3a	C	T	7: 58,926,188 (GRCm39)	T322I	probably damaging	Het
Vac14	T	A	8: 111,439,430 (GRCm39)	V669E	probably damaging	Het
Zfp658	A	G	7: 43,224,172 (GRCm39)	K816E	possibly damaging	Het
Zfp846	T	C	9: 20,499,188 (GRCm39)	M1T	probably null	Het

Other mutations in Vmn1r225

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Vmn1r225	APN	17	20,723,081 (GRCm39)	missense	probably damaging	1.00
IGL01830:Vmn1r225	APN	17	20,722,717 (GRCm39)	missense	probably damaging	0.96
IGL02943:Vmn1r225	APN	17	20,722,567 (GRCm39)	missense	possibly damaging	0.67
R0544:Vmn1r225	UTSW	17	20,722,718 (GRCm39)	missense	probably benign	0.44
R1126:Vmn1r225	UTSW	17	20,722,588 (GRCm39)	missense	probably benign	0.03
R1809:Vmn1r225	UTSW	17	20,722,918 (GRCm39)	missense	probably benign	0.04
R1928:Vmn1r225	UTSW	17	20,723,071 (GRCm39)	missense	probably benign	0.00
R2044:Vmn1r225	UTSW	17	20,722,852 (GRCm39)	missense	possibly damaging	0.50
R2191:Vmn1r225	UTSW	17	20,723,147 (GRCm39)	missense	probably damaging	0.98
R2206:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R2207:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R2680:Vmn1r225	UTSW	17	20,723,055 (GRCm39)	missense	probably benign	0.00
R3740:Vmn1r225	UTSW	17	20,723,261 (GRCm39)	missense	possibly damaging	0.56
R3807:Vmn1r225	UTSW	17	20,723,114 (GRCm39)	nonsense	probably null
R4196:Vmn1r225	UTSW	17	20,723,237 (GRCm39)	missense	probably benign	0.00
R4970:Vmn1r225	UTSW	17	20,722,831 (GRCm39)	missense	possibly damaging	0.74
R5129:Vmn1r225	UTSW	17	20,723,378 (GRCm39)	missense	probably damaging	1.00
R5130:Vmn1r225	UTSW	17	20,723,047 (GRCm39)	missense	possibly damaging	0.81
R5187:Vmn1r225	UTSW	17	20,723,177 (GRCm39)	missense	probably damaging	0.96
R5580:Vmn1r225	UTSW	17	20,723,101 (GRCm39)	missense	probably damaging	1.00
R6563:Vmn1r225	UTSW	17	20,722,763 (GRCm39)	missense	probably benign	0.03
R6674:Vmn1r225	UTSW	17	20,723,377 (GRCm39)	missense	probably benign	0.06
R7143:Vmn1r225	UTSW	17	20,722,646 (GRCm39)	missense	probably benign	0.22
R7422:Vmn1r225	UTSW	17	20,723,059 (GRCm39)	missense	probably benign	0.25
R7651:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R7952:Vmn1r225	UTSW	17	20,722,589 (GRCm39)	missense	probably damaging	0.98
R8097:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R8696:Vmn1r225	UTSW	17	20,723,419 (GRCm39)	missense	probably damaging	1.00
R8823:Vmn1r225	UTSW	17	20,722,823 (GRCm39)	missense	probably benign	0.08
R9007:Vmn1r225	UTSW	17	20,723,449 (GRCm39)	missense	probably damaging	0.96
R9041:Vmn1r225	UTSW	17	20,722,577 (GRCm39)	missense	possibly damaging	0.53
R9147:Vmn1r225	UTSW	17	20,722,577 (GRCm39)	missense	possibly damaging	0.53
R9148:Vmn1r225	UTSW	17	20,722,577 (GRCm39)	missense	possibly damaging	0.53
R9312:Vmn1r225	UTSW	17	20,722,960 (GRCm39)	missense	probably benign	0.39
R9401:Vmn1r225	UTSW	17	20,722,912 (GRCm39)	missense	probably damaging	1.00
R9401:Vmn1r225	UTSW	17	20,722,911 (GRCm39)	nonsense	probably null
R9488:Vmn1r225	UTSW	17	20,722,793 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn1r225	UTSW	17	20,722,753 (GRCm39)	missense	possibly damaging	0.65

Predicted Primers

PCR Primer
(F):5'- TGTCTCCTGAAAACAGAGCC -3'
(R):5'- AACTGGTGAAGATCCTGTGAC -3'

Sequencing Primer
(F):5'- TCTCCTGAAAACAGAGCCACACAG -3'
(R):5'- CTGGTGAAGATCCTGTGACTATAGAC -3'

Posted On

2019-05-13