Mutagenetix > Incidental Mutation

Incidental Mutation 'R7010:Vmn2r1'

544954

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r1

Ensembl Gene

ENSMUSG00000027824

Gene Name

vomeronasal 2, receptor 1

Synonyms

V2r83, EG56544

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.133) question?

Stock #

R7010 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

63988968-64016905 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 64012146 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 669 (T669I)

Ref Sequence

ENSEMBL: ENSMUSP00000029406 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029406]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000029406
AA Change: T669I

PolyPhen 2 Score 0.187 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000029406
Gene: ENSMUSG00000027824
AA Change: T669I

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	86	504	6e-92	PFAM
Pfam:NCD3G	546	599	2.4e-17	PFAM
Pfam:7tm_3	632	866	4.1e-48	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
AL592187.3	A	T	15: 77,486,797 (GRCm39)	Y58F	probably benign	Het
Ano10	T	C	9: 122,082,190 (GRCm39)	T494A	probably damaging	Het
Asah2	A	T	19: 32,031,954 (GRCm39)	F72I	probably benign	Het
Atat1	T	C	17: 36,219,522 (GRCm39)	D114G	probably damaging	Het
Atp6v1e2	C	T	17: 87,251,773 (GRCm39)	M208I	probably benign	Het
Bicd1	A	G	6: 149,396,113 (GRCm39)	Y161C	probably damaging	Het
Camk2g	T	C	14: 20,791,512 (GRCm39)	S410G	probably benign	Het
Car2	C	T	3: 14,965,113 (GRCm39)	P249L	possibly damaging	Het
Cdh23	T	G	10: 60,366,770 (GRCm39)	I237L	probably benign	Het
Dlat	T	A	9: 50,569,274 (GRCm39)	K176N	probably damaging	Het
Dnajc12	T	A	10: 63,233,059 (GRCm39)	C67S	probably benign	Het
Fat1	G	T	8: 45,406,386 (GRCm39)	E1046*	probably null	Het
Gmip	G	A	8: 70,264,050 (GRCm39)	A137T	probably damaging	Het
Gpatch2l	G	A	12: 86,290,958 (GRCm39)	R47H	probably damaging	Het
Grk6	A	G	13: 55,598,113 (GRCm39)	I62V	possibly damaging	Het
Hook1	G	T	4: 95,903,048 (GRCm39)	L512F	probably damaging	Het
Ighe	T	C	12: 113,236,761 (GRCm39)	T36A		Het
Il17rc	A	G	6: 113,456,249 (GRCm39)	N338S	possibly damaging	Het
Itgb6	T	C	2: 60,480,322 (GRCm39)	Y338C	probably damaging	Het
Kcnd2	A	G	6: 21,216,707 (GRCm39)	Y137C	probably damaging	Het
L3mbtl3	T	A	10: 26,158,759 (GRCm39)		probably null	Het
Lcn3	T	C	2: 25,656,068 (GRCm39)	F41S	probably damaging	Het
Map3k8	T	C	18: 4,334,060 (GRCm39)	H344R	probably damaging	Het
Marf1	A	T	16: 13,954,865 (GRCm39)	I884N	probably damaging	Het
Nalcn	G	A	14: 123,530,877 (GRCm39)	T1387I	probably damaging	Het
Nrros	T	C	16: 31,962,398 (GRCm39)	T540A	probably damaging	Het
Or5p63	A	T	7: 107,811,349 (GRCm39)	I129N	probably damaging	Het
Pank2	T	C	2: 131,122,293 (GRCm39)	Y273H	probably benign	Het
Pgrmc2	A	G	3: 41,037,068 (GRCm39)	V121A	probably damaging	Het
Phldb2	C	T	16: 45,571,868 (GRCm39)	V1175M	probably damaging	Het
Prss3b	A	C	6: 41,009,247 (GRCm39)	S196A	probably benign	Het
Ranbp2	A	G	10: 58,290,393 (GRCm39)		probably null	Het
Rsrc1	C	T	3: 66,901,982 (GRCm39)	P44L	unknown	Het
Syt7	A	G	19: 10,395,354 (GRCm39)	T55A	probably benign	Het
Tfcp2l1	T	C	1: 118,581,457 (GRCm39)	S137P	probably damaging	Het
Tom1	T	A	8: 75,778,603 (GRCm39)	V140D	probably damaging	Het
Ttc23l	T	G	15: 10,515,224 (GRCm39)	I385L	probably damaging	Het
Vmn1r122	A	G	7: 20,867,896 (GRCm39)	V53A	probably damaging	Het
Vmn2r86	A	G	10: 130,291,726 (GRCm39)	L13P	probably benign	Het
Zfp958	T	C	8: 4,678,377 (GRCm39)	I134T	probably benign	Het

Other mutations in Vmn2r1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00233:Vmn2r1	APN	3	64,012,389 (GRCm39)	nonsense	probably null
IGL00335:Vmn2r1	APN	3	64,012,809 (GRCm39)	missense	probably damaging	0.96
IGL01509:Vmn2r1	APN	3	64,010,466 (GRCm39)	missense	probably benign	0.03
IGL01641:Vmn2r1	APN	3	64,011,924 (GRCm39)	missense	probably benign	0.19
IGL01656:Vmn2r1	APN	3	63,989,274 (GRCm39)	missense	probably damaging	0.96
IGL01927:Vmn2r1	APN	3	63,989,105 (GRCm39)	missense	probably benign	0.01
IGL02093:Vmn2r1	APN	3	64,012,130 (GRCm39)	missense	probably benign
IGL02146:Vmn2r1	APN	3	64,012,104 (GRCm39)	missense	probably benign	0.39
IGL02186:Vmn2r1	APN	3	63,989,138 (GRCm39)	missense	probably benign	0.01
IGL02320:Vmn2r1	APN	3	63,989,180 (GRCm39)	missense	possibly damaging	0.61
IGL02423:Vmn2r1	APN	3	63,997,665 (GRCm39)	missense	probably benign	0.00
IGL02709:Vmn2r1	APN	3	64,012,355 (GRCm39)	missense	probably benign	0.24
R0034:Vmn2r1	UTSW	3	63,997,435 (GRCm39)	missense	probably damaging	1.00
R0064:Vmn2r1	UTSW	3	64,012,209 (GRCm39)	missense	possibly damaging	0.76
R0152:Vmn2r1	UTSW	3	63,989,240 (GRCm39)	missense	possibly damaging	0.92
R0305:Vmn2r1	UTSW	3	63,997,087 (GRCm39)	missense	probably damaging	1.00
R0314:Vmn2r1	UTSW	3	63,993,980 (GRCm39)	missense	probably damaging	1.00
R0317:Vmn2r1	UTSW	3	63,989,240 (GRCm39)	missense	possibly damaging	0.92
R0465:Vmn2r1	UTSW	3	63,989,180 (GRCm39)	missense	possibly damaging	0.61
R1144:Vmn2r1	UTSW	3	63,997,541 (GRCm39)	missense	probably damaging	1.00
R1163:Vmn2r1	UTSW	3	63,994,046 (GRCm39)	missense	probably benign	0.03
R1448:Vmn2r1	UTSW	3	64,008,734 (GRCm39)	missense	probably damaging	1.00
R1491:Vmn2r1	UTSW	3	63,997,034 (GRCm39)	missense	probably damaging	1.00
R1543:Vmn2r1	UTSW	3	63,996,994 (GRCm39)	missense	probably damaging	1.00
R1611:Vmn2r1	UTSW	3	64,011,958 (GRCm39)	nonsense	probably null
R1676:Vmn2r1	UTSW	3	63,997,603 (GRCm39)	nonsense	probably null
R1727:Vmn2r1	UTSW	3	63,989,163 (GRCm39)	missense	probably benign
R1851:Vmn2r1	UTSW	3	64,008,926 (GRCm39)	missense	probably benign	0.32
R3080:Vmn2r1	UTSW	3	63,997,205 (GRCm39)	missense	probably damaging	1.00
R3790:Vmn2r1	UTSW	3	63,994,185 (GRCm39)	critical splice donor site	probably null
R4111:Vmn2r1	UTSW	3	63,997,176 (GRCm39)	missense	probably benign	0.23
R4689:Vmn2r1	UTSW	3	64,012,074 (GRCm39)	missense	possibly damaging	0.94
R4747:Vmn2r1	UTSW	3	63,989,267 (GRCm39)	missense	probably benign	0.00
R4970:Vmn2r1	UTSW	3	63,997,544 (GRCm39)	missense	possibly damaging	0.81
R5033:Vmn2r1	UTSW	3	64,012,501 (GRCm39)	missense	probably damaging	1.00
R5086:Vmn2r1	UTSW	3	63,997,418 (GRCm39)	missense	probably benign	0.00
R5112:Vmn2r1	UTSW	3	63,997,544 (GRCm39)	missense	possibly damaging	0.81
R5385:Vmn2r1	UTSW	3	64,008,819 (GRCm39)	missense	possibly damaging	0.89
R5629:Vmn2r1	UTSW	3	64,012,538 (GRCm39)	missense	possibly damaging	0.87
R5762:Vmn2r1	UTSW	3	63,997,474 (GRCm39)	missense	probably benign	0.24
R5867:Vmn2r1	UTSW	3	64,011,990 (GRCm39)	missense	probably benign
R5893:Vmn2r1	UTSW	3	63,993,974 (GRCm39)	missense	probably damaging	1.00
R6037:Vmn2r1	UTSW	3	63,989,150 (GRCm39)	missense	probably benign	0.00
R6037:Vmn2r1	UTSW	3	63,989,150 (GRCm39)	missense	probably benign	0.00
R6290:Vmn2r1	UTSW	3	64,012,873 (GRCm39)	missense	probably benign	0.02
R6443:Vmn2r1	UTSW	3	64,012,374 (GRCm39)	missense	possibly damaging	0.78
R6464:Vmn2r1	UTSW	3	64,008,766 (GRCm39)	missense	probably benign
R6826:Vmn2r1	UTSW	3	64,012,567 (GRCm39)	nonsense	probably null
R6874:Vmn2r1	UTSW	3	64,012,376 (GRCm39)	missense	probably damaging	1.00
R6882:Vmn2r1	UTSW	3	63,997,529 (GRCm39)	missense	possibly damaging	0.64
R6983:Vmn2r1	UTSW	3	63,989,118 (GRCm39)	missense	probably benign
R7144:Vmn2r1	UTSW	3	63,997,362 (GRCm39)	missense	probably damaging	1.00
R7341:Vmn2r1	UTSW	3	64,012,877 (GRCm39)	makesense	probably null
R7510:Vmn2r1	UTSW	3	63,993,922 (GRCm39)	missense	probably damaging	1.00
R7557:Vmn2r1	UTSW	3	63,997,475 (GRCm39)	missense	probably damaging	0.99
R7895:Vmn2r1	UTSW	3	63,997,130 (GRCm39)	missense	possibly damaging	0.90
R8108:Vmn2r1	UTSW	3	64,010,471 (GRCm39)	missense	probably damaging	0.98
R8209:Vmn2r1	UTSW	3	63,997,199 (GRCm39)	missense	possibly damaging	0.64
R8365:Vmn2r1	UTSW	3	63,994,034 (GRCm39)	missense	possibly damaging	0.80
R8514:Vmn2r1	UTSW	3	63,993,942 (GRCm39)	missense	probably benign	0.11
R8554:Vmn2r1	UTSW	3	63,997,334 (GRCm39)	missense	probably damaging	0.98
R8980:Vmn2r1	UTSW	3	64,010,501 (GRCm39)	missense
R9140:Vmn2r1	UTSW	3	63,997,465 (GRCm39)	missense	probably benign	0.05
R9239:Vmn2r1	UTSW	3	64,011,959 (GRCm39)	missense	probably damaging	0.99
R9441:Vmn2r1	UTSW	3	64,012,674 (GRCm39)	missense	probably damaging	1.00
R9549:Vmn2r1	UTSW	3	63,997,493 (GRCm39)	missense	probably benign	0.17
R9771:Vmn2r1	UTSW	3	63,997,559 (GRCm39)	missense	possibly damaging	0.79
X0065:Vmn2r1	UTSW	3	63,997,678 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCTCGCTTATGAAGAGGCAC -3'
(R):5'- GCTCCAACACCAAGTATGCTG -3'

Sequencing Primer
(F):5'- AAGAGGCACTGGGGTTCACTC -3'
(R):5'- GTATGCTGCACATATACCAATCTC -3'

Posted On

2019-05-13