Mutagenetix > Incidental Mutation

Incidental Mutation 'R7012:Vmn2r97'

545100

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r97

Ensembl Gene

ENSMUSG00000091491

Gene Name

vomeronasal 2, receptor 97

Synonyms

EG627367

MMRRC Submission

045113-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R7012 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19134584-19168333 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 19167756 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 670 (V670A)

Ref Sequence

ENSEMBL: ENSMUSP00000129313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168710] [ENSMUST00000232219]

AlphaFold

K7N6Z2

Predicted Effect

probably damaging
Transcript: ENSMUST00000168710
AA Change: V670A

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000129313
Gene: ENSMUSG00000091491
AA Change: V670A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	82	442	2.9e-36	PFAM
Pfam:NCD3G	513	566	4.9e-21	PFAM
Pfam:7tm_3	599	834	1.7e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000232219

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox3	T	C	5: 35,769,431 (GRCm39)	F686L	probably benign	Het
Adcy4	C	T	14: 56,017,376 (GRCm39)	V266I	possibly damaging	Het
Adgrb1	A	G	15: 74,401,750 (GRCm39)	T249A	probably damaging	Het
Adss1	A	G	12: 112,600,670 (GRCm39)	D213G	probably benign	Het
Ap1b1	T	G	11: 4,980,963 (GRCm39)	V453G	probably damaging	Het
Apold1	G	A	6: 134,961,007 (GRCm39)	G154R	probably damaging	Het
Birc5	A	G	11: 117,740,262 (GRCm39)	E29G	probably benign	Het
Clcn1	G	A	6: 42,267,542 (GRCm39)	R75H	probably benign	Het
Cngb1	T	A	8: 95,984,583 (GRCm39)	I868F	possibly damaging	Het
Cntn6	T	A	6: 104,703,223 (GRCm39)	V215E	probably damaging	Het
Cntn6	A	G	6: 104,751,441 (GRCm39)	I294V	probably benign	Het
Col6a2	A	T	10: 76,450,511 (GRCm39)	I140N	possibly damaging	Het
Cops5	A	G	1: 10,100,890 (GRCm39)	*147Q	probably null	Het
Dbr1	T	A	9: 99,465,374 (GRCm39)	Y317*	probably null	Het
Dock5	A	C	14: 68,060,035 (GRCm39)	V468G	probably damaging	Het
F13b	A	G	1: 139,444,096 (GRCm39)	I477V	probably benign	Het
Fhad1	CGG	CG	4: 141,645,602 (GRCm39)		probably null	Het
Git1	T	C	11: 77,390,606 (GRCm39)	L114P	probably damaging	Het
Greb1l	G	T	18: 10,529,707 (GRCm39)		probably null	Het
Itih4	A	G	14: 30,612,706 (GRCm39)	N244S	probably benign	Het
Lin28a	A	G	4: 133,746,040 (GRCm39)	S5P	probably damaging	Het
Lipt1	T	C	1: 37,915,060 (GRCm39)	I372T	probably benign	Het
Lysmd4	A	G	7: 66,875,765 (GRCm39)	T143A	probably benign	Het
Muc16	T	C	9: 18,406,914 (GRCm39)		probably null	Het
Or13c9	A	G	4: 52,936,193 (GRCm39)	L30P	probably damaging	Het
Or2y1g	A	T	11: 49,171,823 (GRCm39)	M283L	probably benign	Het
Or5t16	A	T	2: 86,819,051 (GRCm39)	H156Q	possibly damaging	Het
Or6e1	A	G	14: 54,519,674 (GRCm39)	I226T	possibly damaging	Het
Pclo	G	A	5: 14,800,493 (GRCm39)	G4438D	unknown	Het
Phlpp2	T	A	8: 110,603,486 (GRCm39)	F51I	possibly damaging	Het
Rab5c	G	A	11: 100,610,789 (GRCm39)	R40C	probably damaging	Het
Rxfp2	T	C	5: 150,004,659 (GRCm39)	V711A	probably benign	Het
Sbno2	A	T	10: 79,905,352 (GRCm39)		probably benign	Het
Setd2	T	A	9: 110,376,751 (GRCm39)	S189T	probably damaging	Het
Sez6	A	G	11: 77,868,621 (GRCm39)	N965S	probably benign	Het
Sh3d19	A	G	3: 85,992,320 (GRCm39)	N116S	probably benign	Het
Slc43a3	T	C	2: 84,777,313 (GRCm39)	Y221H	probably damaging	Het
Slco1a6	T	C	6: 142,032,287 (GRCm39)	I613V	probably benign	Het
Stag3	T	A	5: 138,295,871 (GRCm39)		probably null	Het
Ston1	T	C	17: 88,943,413 (GRCm39)	M273T	probably damaging	Het
Tbc1d32	A	T	10: 56,100,820 (GRCm39)	Y53N	probably damaging	Het
Tmem132b	T	A	5: 125,775,654 (GRCm39)	L376Q	probably damaging	Het
Trim60	A	G	8: 65,453,043 (GRCm39)	V402A	possibly damaging	Het
Tssk5	A	C	15: 76,257,745 (GRCm39)	N178K	probably damaging	Het
Ttll9	T	C	2: 152,844,982 (GRCm39)	I450T	possibly damaging	Het
Tyw1	T	G	5: 130,306,571 (GRCm39)		probably null	Het
Usp16	T	C	16: 87,255,632 (GRCm39)		probably null	Het
Vmn2r98	A	G	17: 19,286,530 (GRCm39)	N343D	probably benign	Het
Zfp472	T	G	17: 33,196,220 (GRCm39)	N98K	probably benign	Het

Other mutations in Vmn2r97

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00897:Vmn2r97	APN	17	19,167,921 (GRCm39)	missense	probably benign	0.37
IGL00962:Vmn2r97	APN	17	19,149,490 (GRCm39)	missense	probably damaging	1.00
IGL01704:Vmn2r97	APN	17	19,168,073 (GRCm39)	missense	probably damaging	0.99
IGL01888:Vmn2r97	APN	17	19,149,286 (GRCm39)	nonsense	probably null
IGL02429:Vmn2r97	APN	17	19,150,596 (GRCm39)	missense	possibly damaging	0.94
IGL02742:Vmn2r97	APN	17	19,149,432 (GRCm39)	missense	probably damaging	0.97
IGL02934:Vmn2r97	APN	17	19,149,947 (GRCm39)	missense	probably benign	0.00
IGL02978:Vmn2r97	APN	17	19,168,298 (GRCm39)	missense	probably benign	0.01
IGL03230:Vmn2r97	APN	17	19,149,668 (GRCm39)	missense	probably benign	0.10
IGL03241:Vmn2r97	APN	17	19,148,438 (GRCm39)	missense	probably benign	0.11
IGL03050:Vmn2r97	UTSW	17	19,167,900 (GRCm39)	missense	possibly damaging	0.84
PIT4469001:Vmn2r97	UTSW	17	19,149,878 (GRCm39)	missense	probably benign	0.00
R0482:Vmn2r97	UTSW	17	19,167,930 (GRCm39)	missense	probably damaging	1.00
R0514:Vmn2r97	UTSW	17	19,134,734 (GRCm39)	missense	probably benign	0.25
R0944:Vmn2r97	UTSW	17	19,167,665 (GRCm39)	missense	probably benign	0.13
R1061:Vmn2r97	UTSW	17	19,148,440 (GRCm39)	nonsense	probably null
R1546:Vmn2r97	UTSW	17	19,168,110 (GRCm39)	missense	probably damaging	1.00
R1725:Vmn2r97	UTSW	17	19,149,397 (GRCm39)	missense	probably benign	0.43
R1860:Vmn2r97	UTSW	17	19,167,648 (GRCm39)	missense	probably benign	0.01
R1938:Vmn2r97	UTSW	17	19,149,593 (GRCm39)	missense	probably benign	0.01
R1944:Vmn2r97	UTSW	17	19,160,500 (GRCm39)	missense	probably benign	0.00
R2027:Vmn2r97	UTSW	17	19,149,944 (GRCm39)	missense	unknown
R2106:Vmn2r97	UTSW	17	19,168,100 (GRCm39)	missense	probably damaging	1.00
R2151:Vmn2r97	UTSW	17	19,167,584 (GRCm39)	nonsense	probably null
R2153:Vmn2r97	UTSW	17	19,167,584 (GRCm39)	nonsense	probably null
R2154:Vmn2r97	UTSW	17	19,167,584 (GRCm39)	nonsense	probably null
R2516:Vmn2r97	UTSW	17	19,167,814 (GRCm39)	missense	probably benign
R3739:Vmn2r97	UTSW	17	19,148,413 (GRCm39)	missense	probably damaging	1.00
R3744:Vmn2r97	UTSW	17	19,149,890 (GRCm39)	missense	probably benign
R3885:Vmn2r97	UTSW	17	19,148,596 (GRCm39)	missense	possibly damaging	0.90
R3899:Vmn2r97	UTSW	17	19,167,873 (GRCm39)	missense	probably damaging	0.96
R4115:Vmn2r97	UTSW	17	19,148,332 (GRCm39)	missense	probably benign	0.01
R4247:Vmn2r97	UTSW	17	19,167,542 (GRCm39)	missense	possibly damaging	0.83
R4287:Vmn2r97	UTSW	17	19,168,337 (GRCm39)	intron	probably benign
R4439:Vmn2r97	UTSW	17	19,150,616 (GRCm39)	missense	probably benign	0.00
R4523:Vmn2r97	UTSW	17	19,149,333 (GRCm39)	missense	probably benign	0.03
R4783:Vmn2r97	UTSW	17	19,149,550 (GRCm39)	missense	probably benign
R4948:Vmn2r97	UTSW	17	19,167,561 (GRCm39)	missense	possibly damaging	0.95
R4981:Vmn2r97	UTSW	17	19,160,436 (GRCm39)	nonsense	probably null
R5029:Vmn2r97	UTSW	17	19,168,173 (GRCm39)	missense	probably damaging	1.00
R5200:Vmn2r97	UTSW	17	19,148,615 (GRCm39)	missense	probably damaging	1.00
R5541:Vmn2r97	UTSW	17	19,148,617 (GRCm39)	nonsense	probably null
R5637:Vmn2r97	UTSW	17	19,167,628 (GRCm39)	nonsense	probably null
R5765:Vmn2r97	UTSW	17	19,167,442 (GRCm39)	nonsense	probably null
R5885:Vmn2r97	UTSW	17	19,168,035 (GRCm39)	missense	possibly damaging	0.50
R6272:Vmn2r97	UTSW	17	19,167,861 (GRCm39)	missense	possibly damaging	0.70
R6553:Vmn2r97	UTSW	17	19,150,566 (GRCm39)	nonsense	probably null
R6818:Vmn2r97	UTSW	17	19,168,193 (GRCm39)	missense	possibly damaging	0.95
R6880:Vmn2r97	UTSW	17	19,134,770 (GRCm39)	missense	probably benign	0.00
R7023:Vmn2r97	UTSW	17	19,134,663 (GRCm39)	missense	probably damaging	1.00
R7044:Vmn2r97	UTSW	17	19,134,629 (GRCm39)	missense	probably benign	0.05
R7191:Vmn2r97	UTSW	17	19,150,548 (GRCm39)	missense	probably damaging	1.00
R7503:Vmn2r97	UTSW	17	19,148,470 (GRCm39)	missense	probably benign
R7862:Vmn2r97	UTSW	17	19,167,416 (GRCm39)	missense	probably damaging	1.00
R7876:Vmn2r97	UTSW	17	19,149,326 (GRCm39)	missense	probably damaging	0.97
R7890:Vmn2r97	UTSW	17	19,149,802 (GRCm39)	missense	probably benign	0.00
R7936:Vmn2r97	UTSW	17	19,150,662 (GRCm39)	missense	probably damaging	1.00
R7978:Vmn2r97	UTSW	17	19,167,854 (GRCm39)	missense	probably damaging	1.00
R8405:Vmn2r97	UTSW	17	19,134,802 (GRCm39)	critical splice donor site	probably null
R8755:Vmn2r97	UTSW	17	19,168,104 (GRCm39)	missense	probably damaging	1.00
R8790:Vmn2r97	UTSW	17	19,160,472 (GRCm39)	missense	probably damaging	1.00
R8850:Vmn2r97	UTSW	17	19,149,607 (GRCm39)	missense	probably benign	0.00
R9060:Vmn2r97	UTSW	17	19,134,585 (GRCm39)	start codon destroyed	probably null	0.94
R9079:Vmn2r97	UTSW	17	19,149,640 (GRCm39)	missense	probably benign
R9252:Vmn2r97	UTSW	17	19,167,849 (GRCm39)	missense	probably benign	0.00
R9278:Vmn2r97	UTSW	17	19,134,762 (GRCm39)	missense	probably benign	0.00
R9342:Vmn2r97	UTSW	17	19,149,368 (GRCm39)	missense	probably benign
R9422:Vmn2r97	UTSW	17	19,149,333 (GRCm39)	missense	probably benign	0.03
R9496:Vmn2r97	UTSW	17	19,149,227 (GRCm39)	missense	probably damaging	1.00
R9571:Vmn2r97	UTSW	17	19,149,919 (GRCm39)	missense	probably benign
R9601:Vmn2r97	UTSW	17	19,134,770 (GRCm39)	missense	probably benign
R9672:Vmn2r97	UTSW	17	19,149,442 (GRCm39)	missense	probably benign	0.00
R9773:Vmn2r97	UTSW	17	19,168,221 (GRCm39)	missense	probably benign	0.01
R9795:Vmn2r97	UTSW	17	19,167,561 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGACACTCCTATTGTCAAGGC -3'
(R):5'- AACAGCTGAGCCCTTGTTGC -3'

Sequencing Primer
(F):5'- TGTCAAGGCAAATAATCGAGCTCTG -3'
(R):5'- GCTGAGCCCTTGTTGCATAAAATG -3'

Posted On

2019-05-13