Mutagenetix > Incidental Mutation

Incidental Mutation 'R7029:Scrn2'

546190

Institutional Source

Beutler Lab

Gene Symbol

Scrn2

Ensembl Gene

ENSMUSG00000020877

Gene Name

secernin 2

Synonyms

SES2, D11Moh48

MMRRC Submission

045130-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7029 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

96920764-96924784 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to A at 96921262 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000021251 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021249] [ENSMUST00000021251] [ENSMUST00000153482]

AlphaFold

Q8VCA8

Predicted Effect

unknown
Transcript: ENSMUST00000021249
AA Change: D7E

SMART Domains

Protein: ENSMUSP00000021249
Gene: ENSMUSG00000020877
AA Change: D7E

Domain	Start	End	E-Value	Type
low complexity region	2	14	N/A	INTRINSIC
Pfam:Peptidase_C69	83	254	2e-10	PFAM
coiled coil region	362	395	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000021251

SMART Domains

Protein: ENSMUSP00000021251
Gene: ENSMUSG00000020878

Domain	Start	End	E-Value	Type
SCOP:d1h6ua2	44	150	1e-15	SMART
Blast:LRR	69	91	7e-6	BLAST
low complexity region	179	191	N/A	INTRINSIC
low complexity region	209	225	N/A	INTRINSIC
low complexity region	265	292	N/A	INTRINSIC
low complexity region	297	323	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000153482
AA Change: D7E

SMART Domains

Protein: ENSMUSP00000116606
Gene: ENSMUSG00000020877
AA Change: D7E

Domain	Start	End	E-Value	Type
low complexity region	2	14	N/A	INTRINSIC
Pfam:Peptidase_C69	59	181	2.4e-9	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034J05Rik	T	C	6: 146,853,841 (GRCm39)	Q271R	probably benign	Het
Abhd4	T	A	14: 54,500,164 (GRCm39)	W63R	probably damaging	Het
Adcy5	A	G	16: 35,120,018 (GRCm39)	M1176V	probably null	Het
Bach1	G	A	16: 87,516,179 (GRCm39)	R240Q	probably benign	Het
Brox	G	A	1: 183,065,750 (GRCm39)	P206L	possibly damaging	Het
Ccn3	A	G	15: 54,611,171 (GRCm39)	D102G	possibly damaging	Het
Def6	A	G	17: 28,444,943 (GRCm39)	K447R	probably benign	Het
Dna2	T	C	10: 62,799,773 (GRCm39)	S726P	probably damaging	Het
Ell	G	A	8: 71,031,879 (GRCm39)	V15I	probably damaging	Het
Entrep2	T	A	7: 64,409,075 (GRCm39)	T440S	probably benign	Het
Epha3	T	A	16: 63,593,698 (GRCm39)	D130V	probably benign	Het
Gys1	A	G	7: 45,089,008 (GRCm39)	T200A	possibly damaging	Het
Habp4	A	T	13: 64,309,939 (GRCm39)	H47L	probably benign	Het
Iqcn	G	T	8: 71,161,511 (GRCm39)	V235L	possibly damaging	Het
Kcnj10	C	A	1: 172,196,563 (GRCm39)	R26S	probably benign	Het
Klhl1	T	A	14: 96,755,632 (GRCm39)	D41V	probably benign	Het
Lyn	A	G	4: 3,782,996 (GRCm39)	T410A	probably damaging	Het
Mga	A	G	2: 119,754,031 (GRCm39)	T847A	probably damaging	Het
Mrgpra3	G	T	7: 47,239,290 (GRCm39)	T212N	probably benign	Het
Myh4	T	C	11: 67,137,251 (GRCm39)	F491L	probably benign	Het
Neurl4	T	A	11: 69,801,562 (GRCm39)	I1206N	probably damaging	Het
Pcdhb15	T	A	18: 37,608,621 (GRCm39)	W618R	possibly damaging	Het
Pomc	A	G	12: 4,010,146 (GRCm39)	H129R	probably damaging	Het
Ppm1l	C	A	3: 69,460,399 (GRCm39)	H325Q	probably benign	Het
Psme4	A	G	11: 30,722,474 (GRCm39)		probably benign	Het
Reep2	A	G	18: 34,978,342 (GRCm39)	I74V	probably null	Het
Robo2	T	G	16: 73,745,225 (GRCm39)	E850A	probably damaging	Het
Sfpq	G	A	4: 126,923,675 (GRCm39)	R673K	probably benign	Het
Sh2d3c	T	C	2: 32,644,581 (GRCm39)	*703R	probably null	Het
Spp1	A	G	5: 104,587,167 (GRCm39)	M85V	probably benign	Het
Srebf1	T	C	11: 60,097,810 (GRCm39)	E98G	probably damaging	Het
Srrm4	T	A	5: 116,582,851 (GRCm39)		probably benign	Het
Ticam1	A	T	17: 56,578,154 (GRCm39)	S314T	possibly damaging	Het
Tie1	T	C	4: 118,341,823 (GRCm39)	I209V	possibly damaging	Het
Vapa	G	A	17: 65,889,586 (GRCm39)	R194*	probably null	Het
Vcan	C	T	13: 89,838,360 (GRCm39)	D2395N	probably damaging	Het
Whamm	A	G	7: 81,241,574 (GRCm39)	H295R	probably benign	Het
Zdhhc14	A	G	17: 5,698,186 (GRCm39)	Y85C	probably damaging	Het
Zfp35	T	A	18: 24,136,583 (GRCm39)	F309Y	probably damaging	Het
Zfp423	C	A	8: 88,414,694 (GRCm39)	C1187F	probably damaging	Het
Zfp874b	T	C	13: 67,622,392 (GRCm39)	Y302C	probably damaging	Het

Other mutations in Scrn2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01410:Scrn2	APN	11	96,921,396 (GRCm39)	missense	probably benign	0.03
IGL01580:Scrn2	APN	11	96,922,956 (GRCm39)	missense	probably benign
R1847:Scrn2	UTSW	11	96,923,021 (GRCm39)	missense	probably benign	0.22
R2509:Scrn2	UTSW	11	96,923,992 (GRCm39)	missense	possibly damaging	0.71
R2698:Scrn2	UTSW	11	96,923,122 (GRCm39)	unclassified	probably benign
R4361:Scrn2	UTSW	11	96,923,064 (GRCm39)	missense	probably null	1.00
R4927:Scrn2	UTSW	11	96,924,326 (GRCm39)	critical splice donor site	probably null
R5040:Scrn2	UTSW	11	96,921,709 (GRCm39)	missense	probably damaging	1.00
R5367:Scrn2	UTSW	11	96,923,953 (GRCm39)	missense	possibly damaging	0.95
R5534:Scrn2	UTSW	11	96,921,751 (GRCm39)	missense	probably benign	0.00
R6145:Scrn2	UTSW	11	96,923,679 (GRCm39)	missense	probably benign	0.35
R7082:Scrn2	UTSW	11	96,923,908 (GRCm39)	missense	possibly damaging	0.68
R7165:Scrn2	UTSW	11	96,924,634 (GRCm39)	missense	probably benign
R7468:Scrn2	UTSW	11	96,923,992 (GRCm39)	missense	possibly damaging	0.95
R7568:Scrn2	UTSW	11	96,921,712 (GRCm39)	missense	probably damaging	1.00
R7632:Scrn2	UTSW	11	96,923,968 (GRCm39)	missense	possibly damaging	0.51
R7950:Scrn2	UTSW	11	96,921,755 (GRCm39)	missense	probably damaging	1.00
R8239:Scrn2	UTSW	11	96,923,046 (GRCm39)	missense	probably damaging	1.00
R8398:Scrn2	UTSW	11	96,921,730 (GRCm39)	missense	probably benign	0.07
R8408:Scrn2	UTSW	11	96,921,869 (GRCm39)	missense	probably damaging	1.00
R8872:Scrn2	UTSW	11	96,922,961 (GRCm39)	missense	probably damaging	1.00
R9206:Scrn2	UTSW	11	96,922,962 (GRCm39)	missense	probably damaging	1.00
R9596:Scrn2	UTSW	11	96,923,907 (GRCm39)	missense	probably damaging	0.98
R9643:Scrn2	UTSW	11	96,921,342 (GRCm39)	missense	probably damaging	0.99
R9708:Scrn2	UTSW	11	96,922,928 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GACTCCAAGCCAAGTGCATTG -3'
(R):5'- GAAGGTCTGGGAATAACGTCTC -3'

Sequencing Primer
(F):5'- CTCCAAGCCAAGTGCATTGTTAGTG -3'
(R):5'- TGGGAATAACGTCTCAACCTTC -3'

Posted On

2019-05-13