Mutagenetix > Incidental Mutation

Incidental Mutation 'R7033:Muc4'

546482

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

045134-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R7033 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

32555089-32602761 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

C to A at 32576698 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000096106
AA Change: T2066N

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: T2066N

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Meta Mutation Damage Score

0.0796

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

96% (70/73)

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700024B05Rik	A	G	14: 41,819,374 (GRCm39)	Y105C	probably damaging	Het
Acr	T	C	15: 89,453,703 (GRCm39)	S81P	probably benign	Het
Akr1c14	T	C	13: 4,129,178 (GRCm39)		probably null	Het
Ank2	C	A	3: 126,738,499 (GRCm39)	E2375*	probably null	Het
Avpi1	A	G	19: 42,113,416 (GRCm39)	W14R	probably damaging	Het
Brd4	A	G	17: 32,417,989 (GRCm39)	V55A	probably benign	Het
Casp8ap2	A	G	4: 32,639,392 (GRCm39)	N149D	probably damaging	Het
Ccl25	A	T	8: 4,399,641 (GRCm39)		probably benign	Het
Celf5	A	G	10: 81,298,548 (GRCm39)	L299P	probably damaging	Het
Cfap418	A	G	4: 10,898,014 (GRCm39)	T199A	probably benign	Het
Cfap57	T	G	4: 118,470,323 (GRCm39)	T186P	possibly damaging	Het
Chrm4	A	G	2: 91,758,692 (GRCm39)	M367V	probably benign	Het
Col1a2	A	T	6: 4,516,904 (GRCm39)		probably benign	Het
Crybg3	G	A	16: 59,374,528 (GRCm39)	P2242L	probably damaging	Het
Cspg4	T	C	9: 56,795,358 (GRCm39)	V1031A	probably damaging	Het
Dbnl	C	A	11: 5,748,102 (GRCm39)	P313T	probably benign	Het
Dnah1	A	G	14: 30,986,882 (GRCm39)	F3637L	probably damaging	Het
Dnah7a	A	T	1: 53,518,820 (GRCm39)	I2979N	probably damaging	Het
Dusp10	T	C	1: 183,769,802 (GRCm39)	V256A	possibly damaging	Het
Dync2i1	T	C	12: 116,175,511 (GRCm39)	M889V	probably benign	Het
Erlin2	T	C	8: 27,521,792 (GRCm39)	V164A	probably benign	Het
Fam110a	T	C	2: 151,812,131 (GRCm39)	D213G	probably damaging	Het
Fkbp1a	T	C	2: 151,399,420 (GRCm39)		probably null	Het
Foxg1	G	A	12: 49,431,503 (GRCm39)		probably benign	Het
Gm12185	T	C	11: 48,806,826 (GRCm39)	S122G	probably benign	Het
Gm2042	A	T	12: 87,927,051 (GRCm39)	D456V	probably damaging	Het
Grin2b	A	G	6: 135,900,036 (GRCm39)	Y282H	probably damaging	Het
Gys2	T	C	6: 142,418,448 (GRCm39)	D27G	probably benign	Het
H2-DMa	T	A	17: 34,355,971 (GRCm39)		probably null	Het
Hectd4	T	A	5: 121,502,631 (GRCm39)	I4245N	possibly damaging	Het
Incenp	A	G	19: 9,870,736 (GRCm39)	Y298H	unknown	Het
Ints2	C	T	11: 86,123,911 (GRCm39)	G626R	probably damaging	Het
Kifc1	A	T	17: 34,102,671 (GRCm39)	V314E	probably damaging	Het
Lurap1l	C	T	4: 80,829,604 (GRCm39)	P5S	probably benign	Het
Mtmr11	A	G	3: 96,077,262 (GRCm39)	Y540C	probably damaging	Het
Myh13	A	G	11: 67,260,142 (GRCm39)	E1860G	possibly damaging	Het
Myl10	G	C	5: 136,726,825 (GRCm39)	V70L	probably benign	Het
Nbeal1	G	A	1: 60,350,106 (GRCm39)	G2385D	probably damaging	Het
Ncaph2	G	A	15: 89,255,559 (GRCm39)	A578T	probably benign	Het
Ncr1	T	C	7: 4,341,144 (GRCm39)	V8A	possibly damaging	Het
Nutm1	T	C	2: 112,086,513 (GRCm39)	T73A	probably damaging	Het
Olfm1	G	A	2: 28,119,348 (GRCm39)	D313N	probably damaging	Het
Or11h4	A	G	14: 50,974,164 (GRCm39)	F152L	possibly damaging	Het
Or13f5	A	G	4: 52,826,089 (GRCm39)	M231V	probably benign	Het
Or4a66	T	A	2: 88,531,164 (GRCm39)	N170Y	probably damaging	Het
Otog	T	A	7: 45,916,822 (GRCm39)		probably null	Het
Peak1	G	T	9: 56,166,991 (GRCm39)	D312E	probably damaging	Het
Plekhg1	T	C	10: 3,890,251 (GRCm39)	I331T	probably damaging	Het
Polr1b	T	C	2: 128,957,562 (GRCm39)	V539A	possibly damaging	Het
Polr2a	A	T	11: 69,638,039 (GRCm39)	H143Q	possibly damaging	Het
Ppargc1b	G	T	18: 61,440,785 (GRCm39)	A711D	probably damaging	Het
Prnd	T	A	2: 131,795,362 (GRCm39)	C161S	possibly damaging	Het
Prrt4	A	G	6: 29,171,147 (GRCm39)	L435P	possibly damaging	Het
Psen2	C	T	1: 180,055,085 (GRCm39)		probably null	Het
Psg23	T	C	7: 18,348,669 (GRCm39)	E46G	possibly damaging	Het
Rasgef1b	C	T	5: 99,380,195 (GRCm39)	R350H	probably damaging	Het
Rfxank	G	C	8: 70,590,820 (GRCm39)	P16A	probably benign	Het
Sema6a	A	G	18: 47,381,637 (GRCm39)	I944T	probably damaging	Het
Serpinc1	A	G	1: 160,825,091 (GRCm39)	T313A	probably benign	Het
Slc27a4	T	C	2: 29,694,283 (GRCm39)	S36P	possibly damaging	Het
Slc36a1	C	A	11: 55,114,563 (GRCm39)	R214S	probably benign	Het
Sorl1	C	T	9: 41,942,279 (GRCm39)	S982N	possibly damaging	Het
Speer1k	G	T	5: 11,000,518 (GRCm39)		probably null	Het
Syt1	A	C	10: 108,526,797 (GRCm39)	D37E	probably benign	Het
Tcl1b5	A	T	12: 105,142,750 (GRCm39)	D26V	probably damaging	Het
Tenm4	A	T	7: 96,544,430 (GRCm39)	K2149*	probably null	Het
Ubc	T	A	5: 125,465,238 (GRCm39)	I30F	probably damaging	Het
Ugt1a7c	A	T	1: 88,023,250 (GRCm39)	E136D	possibly damaging	Het
Utp20	A	G	10: 88,590,337 (GRCm39)		probably null	Het
Vmn1r159	C	T	7: 22,542,289 (GRCm39)	V248I	probably damaging	Het
Vmn2r105	T	C	17: 20,428,874 (GRCm39)	H734R	probably damaging	Het
Xrcc2	T	G	5: 25,897,707 (GRCm39)	I81L	possibly damaging	Het
Zfat	A	C	15: 68,052,864 (GRCm39)	I310S	probably damaging	Het
Zfp119a	A	G	17: 56,173,009 (GRCm39)	V278A	probably benign	Het
Zfp53	A	G	17: 21,720,508 (GRCm39)	K33E	probably benign	Het
Zfp866	G	T	8: 70,218,491 (GRCm39)	H376Q	probably damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,599,186 (GRCm39)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,574,613 (GRCm39)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,569,728 (GRCm39)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,575,465 (GRCm39)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,140 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,139 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,114 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,576,179 (GRCm39)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,575,246 (GRCm39)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,575,362 (GRCm39)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,584,340 (GRCm39)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,569,473 (GRCm39)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,577,001 (GRCm39)	missense	unknown
IGL01625:Muc4	APN	16	32,575,918 (GRCm39)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,555,220 (GRCm39)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,581,722 (GRCm39)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,574,624 (GRCm39)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,570,244 (GRCm39)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,575,383 (GRCm39)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,598,023 (GRCm39)	splice site	probably benign
IGL02210:Muc4	APN	16	32,574,054 (GRCm39)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,569,668 (GRCm39)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,570,894 (GRCm39)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,576,503 (GRCm39)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,596,193 (GRCm39)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,575,656 (GRCm39)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,570,466 (GRCm39)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,569,212 (GRCm39)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,570,866 (GRCm39)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,570,766 (GRCm39)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,570,257 (GRCm39)	nonsense	probably null
IGL03335:Muc4	APN	16	32,574,449 (GRCm39)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,575,436 (GRCm39)	missense	probably benign	0.01
multisplendored	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,590,768 (GRCm39)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,580,625 (GRCm39)	splice site	probably benign
BB004:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
BB014:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
IGL02835:Muc4	UTSW	16	32,584,319 (GRCm39)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,580,622 (GRCm39)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,575,542 (GRCm39)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,576,391 (GRCm39)	missense	unknown
R0119:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,591,978 (GRCm39)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,586,120 (GRCm39)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,574,333 (GRCm39)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,582,910 (GRCm39)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,569,887 (GRCm39)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,577,120 (GRCm39)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,570,925 (GRCm39)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,570,488 (GRCm39)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,590,201 (GRCm39)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,590,221 (GRCm39)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,599,329 (GRCm39)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,576,570 (GRCm39)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,569,343 (GRCm39)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,574,448 (GRCm39)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,574,033 (GRCm39)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,569,167 (GRCm39)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,575,875 (GRCm39)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,577,276 (GRCm39)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,576,307 (GRCm39)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,576,625 (GRCm39)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,576,715 (GRCm39)	missense	unknown
R1928:Muc4	UTSW	16	32,569,350 (GRCm39)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,569,460 (GRCm39)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,570,121 (GRCm39)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,574,054 (GRCm39)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,576,783 (GRCm39)	missense	unknown
R2121:Muc4	UTSW	16	32,580,612 (GRCm39)	missense	unknown
R2139:Muc4	UTSW	16	32,581,599 (GRCm39)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,569,294 (GRCm39)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,587,316 (GRCm39)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,576,265 (GRCm39)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,577,846 (GRCm39)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,575,647 (GRCm39)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,576,200 (GRCm39)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,590,666 (GRCm39)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,577,075 (GRCm39)	missense	unknown
R4523:Muc4	UTSW	16	32,555,154 (GRCm39)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,576,217 (GRCm39)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,577,432 (GRCm39)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,589,651 (GRCm39)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,576,220 (GRCm39)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,570,032 (GRCm39)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,596,277 (GRCm39)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,574,916 (GRCm39)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,570,565 (GRCm39)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,576,472 (GRCm39)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,575,437 (GRCm39)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,575,332 (GRCm39)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,576,670 (GRCm39)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,577,432 (GRCm39)	nonsense	probably null
R5161:Muc4	UTSW	16	32,582,895 (GRCm39)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,570,556 (GRCm39)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,570,484 (GRCm39)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,581,635 (GRCm39)	missense	unknown
R5567:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,569,600 (GRCm39)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,597,181 (GRCm39)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,555,059 (GRCm39)	nonsense	probably null
R5708:Muc4	UTSW	16	32,575,515 (GRCm39)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,570,734 (GRCm39)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,595,213 (GRCm39)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,570,113 (GRCm39)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,570,523 (GRCm39)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,574,480 (GRCm39)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,576,652 (GRCm39)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,579,682 (GRCm39)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,596,157 (GRCm39)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,577,169 (GRCm39)	missense	unknown
R6144:Muc4	UTSW	16	32,587,298 (GRCm39)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,554,958 (GRCm39)	start gained	probably benign
R6268:Muc4	UTSW	16	32,589,141 (GRCm39)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,570,853 (GRCm39)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,575,237 (GRCm39)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,575,476 (GRCm39)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,587,725 (GRCm39)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,555,061 (GRCm39)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,599,320 (GRCm39)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,570,607 (GRCm39)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,579,694 (GRCm39)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,602,378 (GRCm39)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,587,312 (GRCm39)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,596,177 (GRCm39)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,582,892 (GRCm39)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,570,817 (GRCm39)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,570,628 (GRCm39)	nonsense	probably null
R7098:Muc4	UTSW	16	32,577,465 (GRCm39)	missense
R7123:Muc4	UTSW	16	32,569,509 (GRCm39)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,582,862 (GRCm39)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,576,835 (GRCm39)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,575,710 (GRCm39)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,575,740 (GRCm39)	missense	unknown
R7381:Muc4	UTSW	16	32,601,285 (GRCm39)	missense
R7411:Muc4	UTSW	16	32,570,140 (GRCm39)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,587,324 (GRCm39)	missense
R7539:Muc4	UTSW	16	32,576,770 (GRCm39)	missense
R7544:Muc4	UTSW	16	32,555,016 (GRCm39)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,586,076 (GRCm39)	missense
R7596:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,574,161 (GRCm39)	nonsense	probably null
R7639:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,580,479 (GRCm39)	missense
R7651:Muc4	UTSW	16	32,576,949 (GRCm39)	missense
R7688:Muc4	UTSW	16	32,570,278 (GRCm39)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,574,439 (GRCm39)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,589,108 (GRCm39)	missense
R7763:Muc4	UTSW	16	32,574,739 (GRCm39)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,576,568 (GRCm39)	missense	unknown
R7787:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,570,139 (GRCm39)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
R7930:Muc4	UTSW	16	32,575,369 (GRCm39)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,577,123 (GRCm39)	missense
R8096:Muc4	UTSW	16	32,575,764 (GRCm39)	missense	unknown
R8115:Muc4	UTSW	16	32,575,678 (GRCm39)	missense	unknown
R8162:Muc4	UTSW	16	32,569,197 (GRCm39)	missense	unknown
R8220:Muc4	UTSW	16	32,575,701 (GRCm39)	missense	unknown
R8260:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,575,434 (GRCm39)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,576,271 (GRCm39)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,574,201 (GRCm39)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,574,799 (GRCm39)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,574,421 (GRCm39)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,575,629 (GRCm39)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,602,356 (GRCm39)	missense
R8845:Muc4	UTSW	16	32,576,889 (GRCm39)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,569,461 (GRCm39)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,570,280 (GRCm39)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,575,285 (GRCm39)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,574,009 (GRCm39)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,582,910 (GRCm39)	missense
R9034:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,577,842 (GRCm39)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,589,102 (GRCm39)	missense
R9336:Muc4	UTSW	16	32,569,756 (GRCm39)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,569,971 (GRCm39)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,576,992 (GRCm39)	missense
R9429:Muc4	UTSW	16	32,576,098 (GRCm39)	missense	unknown
R9570:Muc4	UTSW	16	32,569,695 (GRCm39)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,577,234 (GRCm39)	missense
R9696:Muc4	UTSW	16	32,574,712 (GRCm39)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,590,644 (GRCm39)	missense
R9743:Muc4	UTSW	16	32,601,194 (GRCm39)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,570,676 (GRCm39)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,570,643 (GRCm39)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,575,855 (GRCm39)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,579,693 (GRCm39)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,576,450 (GRCm39)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,587,767 (GRCm39)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,595,236 (GRCm39)	missense
Z1177:Muc4	UTSW	16	32,587,768 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACGACTCAAGGTTCTACACTTGC -3'
(R):5'- GTGGTGGCAAGTGTAGAACC -3'

Sequencing Primer
(F):5'- AGGTTCTACACTTGCCACCAC -3'
(R):5'- ACCATGAGTCGTTATTACTGTTGAG -3'

Posted On

2019-05-13