Mutagenetix > Incidental Mutation

Incidental Mutation 'R7045:Zfp85'

547271

Institutional Source

Beutler Lab

Gene Symbol

Zfp85

Ensembl Gene

ENSMUSG00000058331

Gene Name

zinc finger protein 85

Synonyms

Zfp85-rs1, KRAB19, Zfp71

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R7045 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

67895919-67903347 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 67897712 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 120 (Y120C)

Ref Sequence

ENSEMBL: ENSMUSP00000121202 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091520] [ENSMUST00000144183]

AlphaFold

Q7TMC9

Predicted Effect

probably benign
Transcript: ENSMUST00000091520

SMART Domains

Protein: ENSMUSP00000089105
Gene: ENSMUSG00000058331

Domain	Start	End	E-Value	Type
KRAB	5	65	5.78e-29	SMART
ZnF_C2H2	81	103	1.95e-3	SMART
ZnF_C2H2	109	131	1.22e-4	SMART
ZnF_C2H2	137	159	6.32e-3	SMART
ZnF_C2H2	165	187	2.95e-3	SMART
ZnF_C2H2	193	215	3.89e-3	SMART
ZnF_C2H2	221	243	3.89e-3	SMART
ZnF_C2H2	249	271	1.1e-2	SMART
ZnF_C2H2	277	299	2.91e-2	SMART
ZnF_C2H2	305	327	2.49e-1	SMART
ZnF_C2H2	333	355	2.09e-3	SMART
ZnF_C2H2	361	383	4.17e-3	SMART
ZnF_C2H2	389	411	2.95e-3	SMART
ZnF_C2H2	417	439	1.3e-4	SMART
ZnF_C2H2	445	467	1.04e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000144183
AA Change: Y120C

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000121202
Gene: ENSMUSG00000058331
AA Change: Y120C

Domain	Start	End	E-Value	Type
KRAB	45	105	5.78e-29	SMART
ZnF_C2H2	121	143	1.95e-3	SMART
ZnF_C2H2	149	171	1.22e-4	SMART
ZnF_C2H2	177	199	6.32e-3	SMART
ZnF_C2H2	205	227	2.95e-3	SMART
ZnF_C2H2	233	255	3.89e-3	SMART
ZnF_C2H2	261	283	3.89e-3	SMART
ZnF_C2H2	289	311	1.1e-2	SMART
ZnF_C2H2	317	339	2.91e-2	SMART
ZnF_C2H2	345	367	2.49e-1	SMART
ZnF_C2H2	373	395	2.09e-3	SMART
ZnF_C2H2	401	423	4.17e-3	SMART
ZnF_C2H2	429	451	2.95e-3	SMART
ZnF_C2H2	457	479	1.3e-4	SMART
ZnF_C2H2	485	507	1.04e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akt1	A	T	12: 112,628,735 (GRCm39)	Y18*	probably null	Het
Ank2	C	A	3: 126,806,393 (GRCm39)	A583S	probably damaging	Het
Aoc1l3	T	C	6: 48,965,546 (GRCm39)	V518A	possibly damaging	Het
Atp8b4	T	C	2: 126,214,115 (GRCm39)	N706S	probably benign	Het
Bace2	A	G	16: 97,200,865 (GRCm39)	N111S	probably damaging	Het
Cnp	A	T	11: 100,471,184 (GRCm39)	R275S	probably benign	Het
Cs	T	A	10: 128,188,586 (GRCm39)	M104K	probably benign	Het
Ctcfl	G	A	2: 172,954,167 (GRCm39)	T310I	probably damaging	Het
Cyp2d40	T	C	15: 82,645,763 (GRCm39)	I81V	probably benign	Het
Ddx19a	A	G	8: 111,719,706 (GRCm39)	V30A	probably benign	Het
Disp1	C	A	1: 182,869,030 (GRCm39)	R1130L	probably damaging	Het
Dock6	G	A	9: 21,733,107 (GRCm39)	A1062V	probably damaging	Het
Dpysl2	T	C	14: 67,067,395 (GRCm39)	D172G	probably benign	Het
Eml2	A	G	7: 18,935,504 (GRCm39)	D638G	probably damaging	Het
Epb41l4b	C	A	4: 57,103,522 (GRCm39)	A105S	possibly damaging	Het
Fat4	A	G	3: 38,942,750 (GRCm39)	I548V	probably benign	Het
Gabrb2	G	A	11: 42,484,758 (GRCm39)	A272T	probably damaging	Het
Gask1a	T	C	9: 121,794,707 (GRCm39)	L287P	probably damaging	Het
Hcn1	C	T	13: 118,111,998 (GRCm39)	P654L	unknown	Het
Hjurp	TCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCTGCT	TCT	1: 88,194,000 (GRCm39)		probably benign	Het
Hk1	C	A	10: 62,122,349 (GRCm39)	G477C	probably damaging	Het
Hspa5	C	T	2: 34,663,204 (GRCm39)	P127L	probably damaging	Het
Kiss1r	A	G	10: 79,755,259 (GRCm39)		probably null	Het
L3mbtl4	G	T	17: 68,768,561 (GRCm39)	R223L	probably benign	Het
Loxl4	G	T	19: 42,595,074 (GRCm39)	N200K	probably damaging	Het
Lrba	T	C	3: 86,192,398 (GRCm39)	V104A	probably benign	Het
Lyst	T	A	13: 13,809,485 (GRCm39)	V385D	probably benign	Het
Lyst	T	A	13: 13,812,293 (GRCm39)	C902S	probably damaging	Het
Mrpl28	T	C	17: 26,345,261 (GRCm39)	F227S	probably benign	Het
Mtmr3	A	T	11: 4,448,896 (GRCm39)	V289E	possibly damaging	Het
Ndst3	A	G	3: 123,465,732 (GRCm39)	V80A	probably damaging	Het
Nid2	C	T	14: 19,829,749 (GRCm39)	A680V	possibly damaging	Het
Nudcd1	T	C	15: 44,269,226 (GRCm39)	N145D	probably benign	Het
Nup210	A	T	6: 91,031,433 (GRCm39)	I812N	probably damaging	Het
Or2ak6	A	T	11: 58,592,495 (GRCm39)		probably benign	Het
Or2g7	C	T	17: 38,378,862 (GRCm39)	H267Y	probably benign	Het
Or2y17	A	T	11: 49,231,757 (GRCm39)	T133S	probably damaging	Het
Or52n4b	A	G	7: 108,144,452 (GRCm39)	K238R	probably damaging	Het
Or5b114-ps1	C	A	19: 13,352,336 (GRCm39)	N3K	probably damaging	Het
Or8c11	T	A	9: 38,289,729 (GRCm39)	M184K	probably damaging	Het
Or8k16	A	G	2: 85,520,255 (GRCm39)	S161G	possibly damaging	Het
Pcdhb6	A	C	18: 37,469,329 (GRCm39)	Q750P	possibly damaging	Het
Plppr4	T	C	3: 117,153,683 (GRCm39)	Y72C	probably damaging	Het
Rasgrf2	G	T	13: 92,159,100 (GRCm39)		probably benign	Het
Sbk2	A	G	7: 4,961,905 (GRCm39)	I127T	probably damaging	Het
Smchd1	A	C	17: 71,722,039 (GRCm39)	S817A	probably benign	Het
Speer4b	A	T	5: 27,705,123 (GRCm39)	N83K	probably damaging	Het
Strc	A	T	2: 121,201,207 (GRCm39)	L1296Q	probably damaging	Het
Thap11	C	A	8: 106,582,215 (GRCm39)	R75S	possibly damaging	Het
Unc13a	A	C	8: 72,111,407 (GRCm39)	L268R	possibly damaging	Het
Zfp39	A	T	11: 58,781,269 (GRCm39)	C498S	unknown	Het
Zfp507	T	C	7: 35,494,978 (GRCm39)	T22A	possibly damaging	Het
Zfp882	G	A	8: 72,667,093 (GRCm39)		probably null	Het

Other mutations in Zfp85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0383:Zfp85	UTSW	13	67,896,791 (GRCm39)	missense	probably benign	0.05
R1187:Zfp85	UTSW	13	67,897,835 (GRCm39)	missense	probably damaging	0.99
R1510:Zfp85	UTSW	13	67,903,084 (GRCm39)	intron	probably benign
R1775:Zfp85	UTSW	13	67,897,823 (GRCm39)	missense	probably damaging	0.99
R1803:Zfp85	UTSW	13	67,899,747 (GRCm39)	missense	probably benign	0.15
R2108:Zfp85	UTSW	13	67,897,003 (GRCm39)	missense	probably benign	0.08
R2412:Zfp85	UTSW	13	67,897,765 (GRCm39)	missense	probably damaging	0.99
R3147:Zfp85	UTSW	13	67,900,612 (GRCm39)	missense	probably damaging	0.96
R4811:Zfp85	UTSW	13	67,897,745 (GRCm39)	missense	probably damaging	0.97
R5308:Zfp85	UTSW	13	67,896,974 (GRCm39)	missense	probably damaging	1.00
R5334:Zfp85	UTSW	13	67,899,803 (GRCm39)	missense	probably damaging	0.99
R5373:Zfp85	UTSW	13	67,897,577 (GRCm39)	missense	probably damaging	1.00
R7391:Zfp85	UTSW	13	67,897,410 (GRCm39)	missense	probably damaging	1.00
R7438:Zfp85	UTSW	13	67,897,064 (GRCm39)	missense	probably benign	0.09
R7544:Zfp85	UTSW	13	67,897,184 (GRCm39)	missense	probably benign	0.02
R8046:Zfp85	UTSW	13	67,897,098 (GRCm39)	nonsense	probably null
R8112:Zfp85	UTSW	13	67,896,893 (GRCm39)	missense	possibly damaging	0.71
R9533:Zfp85	UTSW	13	67,897,722 (GRCm39)	missense	probably benign	0.08
R9615:Zfp85	UTSW	13	67,897,326 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TTGTAGGGTTTCTCTCCAGTATGTAC -3'
(R):5'- GGCAGAGACATGCGGATTT -3'

Sequencing Primer
(F):5'- CTCTTCGGTGAACAATAAGTCCTG -3'
(R):5'- GGAAGGCCTTTGATAGGA -3'

Posted On

2019-05-13