Mutagenetix > Incidental Mutation

Incidental Mutation 'R7048:Ank2'

547417

Institutional Source

Beutler Lab

Gene Symbol

Ank2

Ensembl Gene

ENSMUSG00000032826

Gene Name

ankyrin 2, brain

Synonyms

Gm4392, Ank-2, ankyrin B, Ankyrin-B, Ankyrin-2

MMRRC Submission

045146-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7048 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

126715261-127292999 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 126819267 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 468 (Q468L)

Ref Sequence

ENSEMBL: ENSMUSP00000138730 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000182008] [ENSMUST00000182064] [ENSMUST00000182078] [ENSMUST00000182547] [ENSMUST00000182711] [ENSMUST00000182959]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000182008
AA Change: Q468L

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000138730
Gene: ENSMUSG00000032826
AA Change: Q468L

Domain	Start	End	E-Value	Type
ANK	42	71	1.63e3	SMART
ANK	75	104	1.4e-4	SMART
ANK	108	137	6.76e-7	SMART
ANK	141	170	4.46e-7	SMART
ANK	174	202	8.36e1	SMART
ANK	203	232	1.17e2	SMART
ANK	236	265	1.76e-5	SMART
ANK	269	298	6.76e-7	SMART
ANK	302	331	1.43e-5	SMART
ANK	335	364	3.33e-6	SMART
ANK	368	397	2.02e-5	SMART
ANK	401	430	9.55e-7	SMART
ANK	434	463	1.76e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000182064

SMART Domains

Protein: ENSMUSP00000138620
Gene: ENSMUSG00000032826

Domain	Start	End	E-Value	Type
ANK	9	38	1e1	SMART
ANK	42	71	8.9e-7	SMART
ANK	75	104	4.4e-9	SMART
ANK	108	137	2.8e-9	SMART
ANK	141	169	5.3e-1	SMART
ANK	170	199	7.3e-1	SMART
ANK	211	240	1.1e-7	SMART
ANK	244	273	4.4e-9	SMART
ANK	277	306	9.3e-8	SMART
ANK	310	339	2.1e-8	SMART
ANK	343	372	1.3e-7	SMART
ANK	376	405	6.2e-9	SMART
ANK	409	438	1.1e-7	SMART
ANK	442	471	2.9e-8	SMART
ANK	475	504	1.1e-5	SMART
ANK	508	537	6.5e-6	SMART
ANK	541	570	2.3e-7	SMART
ANK	574	603	2.4e-7	SMART
ANK	607	636	3.2e-9	SMART
ANK	640	669	5.5e-5	SMART
ANK	673	702	1.9e-8	SMART
ANK	706	735	3.3e-9	SMART
low complexity region	755	775	N/A	INTRINSIC
low complexity region	793	806	N/A	INTRINSIC
low complexity region	841	853	N/A	INTRINSIC
ZU5	912	1016	2e-63	SMART
low complexity region	1371	1381	N/A	INTRINSIC
low complexity region	1448	1463	N/A	INTRINSIC
low complexity region	1490	1503	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000182078

SMART Domains

Protein: ENSMUSP00000138753
Gene: ENSMUSG00000032826

Domain	Start	End	E-Value	Type
low complexity region	7	18	N/A	INTRINSIC
low complexity region	114	125	N/A	INTRINSIC
low complexity region	191	209	N/A	INTRINSIC
low complexity region	304	312	N/A	INTRINSIC
low complexity region	478	493	N/A	INTRINSIC
low complexity region	527	544	N/A	INTRINSIC
DEATH	591	685	1e-29	SMART
low complexity region	720	736	N/A	INTRINSIC
low complexity region	848	861	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000182547
AA Change: Q476L

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000138602
Gene: ENSMUSG00000032826
AA Change: Q476L

Domain	Start	End	E-Value	Type
ANK	42	71	1.63e3	SMART
ANK	75	104	1.4e-4	SMART
ANK	108	137	6.76e-7	SMART
ANK	141	170	4.46e-7	SMART
ANK	174	202	8.36e1	SMART
ANK	203	232	1.17e2	SMART
ANK	244	273	1.76e-5	SMART
ANK	277	306	6.76e-7	SMART
ANK	310	339	1.43e-5	SMART
ANK	343	372	3.33e-6	SMART
ANK	376	405	2.02e-5	SMART
ANK	409	438	9.55e-7	SMART
ANK	442	471	1.76e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000182711

SMART Domains

Protein: ENSMUSP00000138781
Gene: ENSMUSG00000032826

Domain	Start	End	E-Value	Type
ANK	26	55	1e1	SMART
ANK	59	88	8.9e-7	SMART
ANK	92	121	4.4e-9	SMART
ANK	125	154	2.8e-9	SMART
ANK	158	186	5.3e-1	SMART
ANK	187	216	7.3e-1	SMART
ANK	228	257	1.1e-7	SMART
ANK	261	290	4.4e-9	SMART
ANK	294	323	9.3e-8	SMART
ANK	327	356	2.1e-8	SMART
ANK	360	389	1.3e-7	SMART
ANK	393	422	6.2e-9	SMART
ANK	426	455	1.1e-7	SMART
ANK	459	488	2.9e-8	SMART
ANK	492	521	1.1e-5	SMART
ANK	525	554	6.5e-6	SMART
ANK	558	587	2.3e-7	SMART
ANK	591	620	5.3e-7	SMART
ANK	624	653	2.4e-7	SMART
ANK	657	686	3.2e-9	SMART
ANK	690	719	5.5e-5	SMART
ANK	723	752	1.9e-8	SMART
ANK	756	785	3.3e-9	SMART
low complexity region	805	825	N/A	INTRINSIC
low complexity region	843	856	N/A	INTRINSIC
ZU5	961	1098	1.1e-50	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000182959

SMART Domains

Protein: ENSMUSP00000138251
Gene: ENSMUSG00000032826

Domain	Start	End	E-Value	Type
ANK	9	38	1.63e3	SMART
ANK	42	71	1.4e-4	SMART
ANK	75	104	6.76e-7	SMART
ANK	108	137	4.46e-7	SMART
ANK	141	169	8.36e1	SMART
ANK	170	199	1.17e2	SMART
ANK	211	240	1.76e-5	SMART
ANK	244	273	6.76e-7	SMART
ANK	277	306	1.43e-5	SMART
ANK	310	339	3.33e-6	SMART
ANK	343	372	2.02e-5	SMART
ANK	376	405	9.55e-7	SMART
ANK	409	438	1.76e-5	SMART
ANK	442	471	4.71e-6	SMART
ANK	475	504	1.7e-3	SMART
ANK	508	537	1.05e-3	SMART
ANK	541	570	3.51e-5	SMART
ANK	574	603	8.65e-5	SMART
ANK	607	636	3.76e-5	SMART
ANK	640	669	5.12e-7	SMART
ANK	673	702	8.39e-3	SMART
ANK	706	735	2.9e-6	SMART
ANK	739	768	5.12e-7	SMART
low complexity region	788	808	N/A	INTRINSIC
low complexity region	826	839	N/A	INTRINSIC
low complexity region	874	886	N/A	INTRINSIC
Pfam:ZU5	945	1028	1.5e-30	PFAM
Pfam:ZU5	1021	1082	4.4e-21	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

97% (66/68)

MGI Phenotype

PHENOTYPE: Homozygous mutation of this gene results in death by postnatal day 8, although some animals survive to P20. Mutant animals display reduced body size, impaired balance and locomotion, brain structure dysmorphologies, abnormal lens, and optic nerve degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl2	T	C	3: 148,552,565 (GRCm39)	D629G	probably damaging	Het
Akap11	T	A	14: 78,749,954 (GRCm39)	Q811L		Het
Ano3	A	T	2: 110,513,116 (GRCm39)	Y626*	probably null	Het
Ap1m1	A	G	8: 73,003,642 (GRCm39)	N114S	probably damaging	Het
Asb3	T	A	11: 31,051,121 (GRCm39)	I525N	probably damaging	Het
Atg4a-ps	A	G	3: 103,552,672 (GRCm39)	I223T	probably damaging	Het
B3gnt7	T	C	1: 86,233,308 (GRCm39)	Y68H	probably benign	Het
Bbs5	T	C	2: 69,484,705 (GRCm39)	I125T	probably benign	Het
Cd180	A	G	13: 102,841,431 (GRCm39)	N159S	probably damaging	Het
Cd37	T	C	7: 44,887,888 (GRCm39)		probably benign	Het
Cdk14	T	A	5: 5,143,005 (GRCm39)	Q242L	probably damaging	Het
Clcn1	T	C	6: 42,284,477 (GRCm39)	V605A	probably damaging	Het
Cped1	G	A	6: 22,119,469 (GRCm39)	M309I	probably benign	Het
Cyp2r1	A	T	7: 114,151,971 (GRCm39)	Y120N	probably damaging	Het
Ddx3y	A	T	Y: 1,279,491 (GRCm39)	S124R	probably benign	Het
Dlec1	A	T	9: 118,972,472 (GRCm39)		probably null	Het
Dnah17	T	C	11: 117,936,944 (GRCm39)	E3420G	possibly damaging	Het
Dnajc13	C	T	9: 104,080,613 (GRCm39)		probably null	Het
Dusp23	A	T	1: 172,459,253 (GRCm39)	Y136*	probably null	Het
Eif4b	A	G	15: 102,001,571 (GRCm39)		probably benign	Het
F13a1	A	G	13: 37,082,117 (GRCm39)	V529A	probably benign	Het
Fhl2	A	G	1: 43,162,808 (GRCm39)	Y236H	probably damaging	Het
Gm826	C	T	2: 160,169,026 (GRCm39)	W94*	probably null	Het
Gpsm2	C	T	3: 108,610,361 (GRCm39)	R33H	probably damaging	Het
Hmcn1	T	C	1: 150,475,404 (GRCm39)		probably null	Het
Ifit1bl2	T	C	19: 34,596,551 (GRCm39)	D355G	probably benign	Het
Itga8	A	G	2: 12,115,895 (GRCm39)	V77A	probably damaging	Het
Kcmf1	C	T	6: 72,826,450 (GRCm39)	R40K	probably damaging	Het
Kdm7a	T	C	6: 39,145,982 (GRCm39)	E315G	probably damaging	Het
Kmt2b	C	T	7: 30,268,731 (GRCm39)	G2666D	probably damaging	Het
Lrrtm1	A	G	6: 77,221,152 (GRCm39)	N203S	probably damaging	Het
Mdn1	A	G	4: 32,767,969 (GRCm39)	N5301D	probably benign	Het
Mest	T	G	6: 30,742,723 (GRCm39)	H108Q	probably damaging	Het
Mettl25b	A	G	3: 87,837,167 (GRCm39)	I36T	probably damaging	Het
Moxd1	C	A	10: 24,157,374 (GRCm39)	D335E	probably damaging	Het
Ncf2	A	C	1: 152,683,921 (GRCm39)	N47H	probably benign	Het
Npc1	A	G	18: 12,337,822 (GRCm39)		probably null	Het
Or14j2	A	T	17: 37,886,114 (GRCm39)	S67T	probably damaging	Het
Pcid2	A	G	8: 13,128,243 (GRCm39)	V386A	probably benign	Het
Phactr2	T	A	10: 13,121,168 (GRCm39)	T444S	probably benign	Het
Plekha7	T	C	7: 115,747,559 (GRCm39)	N710D	probably benign	Het
Ppp1r16b	C	T	2: 158,599,174 (GRCm39)	T382I	probably benign	Het
Pramel15	T	C	4: 144,103,754 (GRCm39)	D124G	probably benign	Het
Ptprz1	A	T	6: 22,961,622 (GRCm39)	Y111F	probably benign	Het
Rbbp8	A	G	18: 11,865,277 (GRCm39)	E722G	possibly damaging	Het
Rimbp3	A	G	16: 17,028,190 (GRCm39)	D538G	probably benign	Het
Rims1	A	G	1: 22,511,901 (GRCm39)	S551P	probably damaging	Het
Rsrc1	A	G	3: 67,088,164 (GRCm39)	D166G	probably damaging	Het
Selenon	T	C	4: 134,270,154 (GRCm39)	N350S	probably benign	Het
Sh3gl2	T	A	4: 85,295,802 (GRCm39)	L168H	probably damaging	Het
Smc3	T	C	19: 53,617,682 (GRCm39)	Y560H	probably benign	Het
Syce1	C	T	7: 140,359,281 (GRCm39)	D147N	possibly damaging	Het
Syt10	A	C	15: 89,675,008 (GRCm39)	V446G	probably damaging	Het
Taar1	T	C	10: 23,796,722 (GRCm39)	L140P	probably benign	Het
Tfpi2	C	A	6: 3,968,032 (GRCm39)	C36F	probably damaging	Het
Thoc5	G	A	11: 4,876,237 (GRCm39)		probably null	Het
Tnrc6c	T	A	11: 117,612,800 (GRCm39)	N319K	probably benign	Het
Trhr2	C	T	8: 123,085,418 (GRCm39)	D189N	probably damaging	Het
Trim42	A	T	9: 97,245,474 (GRCm39)	F442Y	probably damaging	Het
Ubap2	G	A	4: 41,196,033 (GRCm39)	T949I	possibly damaging	Het
Ugt1a8	T	C	1: 88,016,024 (GRCm39)	F146L	probably benign	Het
Vmn2r80	C	A	10: 79,030,153 (GRCm39)	Q660K	probably damaging	Het
Vstm2b	C	T	7: 40,578,800 (GRCm39)	T258I	possibly damaging	Het
Washc2	T	C	6: 116,197,544 (GRCm39)	L259P	possibly damaging	Het
Zscan18	A	T	7: 12,508,671 (GRCm39)		probably benign	Het
Zzef1	T	G	11: 72,757,525 (GRCm39)	Y1193*	probably null	Het

Other mutations in Ank2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01298:Ank2	APN	3	126,753,369 (GRCm39)	missense	possibly damaging	0.80
IGL01652:Ank2	APN	3	126,726,690 (GRCm39)	missense	probably benign	0.00
IGL01969:Ank2	APN	3	126,746,872 (GRCm39)	missense	possibly damaging	0.47
IGL02122:Ank2	APN	3	126,731,523 (GRCm39)	splice site	probably benign
IGL02537:Ank2	APN	3	126,749,565 (GRCm39)	missense	probably damaging	1.00
IGL02858:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL02981:Ank2	APN	3	126,728,211 (GRCm39)	missense	possibly damaging	0.58
IGL02981:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03024:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03074:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03111:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03129:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03174:Ank2	APN	3	126,733,744 (GRCm39)	missense	probably damaging	0.98
IGL03177:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03185:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03188:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03242:Ank2	APN	3	126,722,454 (GRCm39)	missense	possibly damaging	0.90
IGL03244:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03248:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03285:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03304:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03358:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03380:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03389:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03400:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
IGL03409:Ank2	APN	3	126,749,519 (GRCm39)	missense	probably damaging	1.00
ballast	UTSW	3	126,736,782 (GRCm39)	missense	unknown
Chain	UTSW	3	126,740,587 (GRCm39)	intron	probably benign
Deadman	UTSW	3	126,723,471 (GRCm39)	missense	probably benign	0.19
drag	UTSW	3	126,797,631 (GRCm39)	missense	probably damaging	1.00
mooring	UTSW	3	126,728,226 (GRCm39)	missense	possibly damaging	0.73
Treasure	UTSW	3	126,740,398 (GRCm39)	missense	unknown
Windlass	UTSW	3	126,739,798 (GRCm39)	missense	probably benign
R0033:Ank2	UTSW	3	126,898,397 (GRCm39)	splice site	probably benign
R0042:Ank2	UTSW	3	126,730,280 (GRCm39)	missense	probably damaging	0.99
R0042:Ank2	UTSW	3	126,730,280 (GRCm39)	missense	probably damaging	0.99
R0079:Ank2	UTSW	3	126,728,264 (GRCm39)	missense	probably benign	0.01
R0423:Ank2	UTSW	3	126,723,509 (GRCm39)	nonsense	probably null
R0699:Ank2	UTSW	3	126,723,478 (GRCm39)	missense	probably benign	0.00
R0724:Ank2	UTSW	3	126,755,986 (GRCm39)	missense	probably damaging	1.00
R0990:Ank2	UTSW	3	126,728,315 (GRCm39)	missense	possibly damaging	0.64
R1450:Ank2	UTSW	3	126,750,951 (GRCm39)	missense	possibly damaging	0.94
R1500:Ank2	UTSW	3	126,726,631 (GRCm39)	missense	probably benign
R1702:Ank2	UTSW	3	126,749,548 (GRCm39)	missense	probably benign	0.00
R1703:Ank2	UTSW	3	126,723,415 (GRCm39)	missense	probably damaging	1.00
R1710:Ank2	UTSW	3	126,726,709 (GRCm39)	nonsense	probably null
R1743:Ank2	UTSW	3	126,722,324 (GRCm39)	missense	probably damaging	0.99
R1775:Ank2	UTSW	3	126,728,196 (GRCm39)	missense	probably benign	0.00
R1852:Ank2	UTSW	3	126,791,500 (GRCm39)	critical splice donor site	probably null
R2198:Ank2	UTSW	3	126,728,226 (GRCm39)	missense	possibly damaging	0.73
R2892:Ank2	UTSW	3	127,041,892 (GRCm39)	splice site	probably null
R2893:Ank2	UTSW	3	127,041,892 (GRCm39)	splice site	probably null
R2894:Ank2	UTSW	3	127,041,892 (GRCm39)	splice site	probably null
R3148:Ank2	UTSW	3	126,726,724 (GRCm39)	missense	probably benign	0.00
R3776:Ank2	UTSW	3	126,735,911 (GRCm39)	intron	probably benign
R3784:Ank2	UTSW	3	126,746,842 (GRCm39)	missense	probably damaging	1.00
R3856:Ank2	UTSW	3	126,723,493 (GRCm39)	missense	probably benign	0.00
R3906:Ank2	UTSW	3	126,810,547 (GRCm39)	missense	probably damaging	1.00
R3907:Ank2	UTSW	3	126,810,547 (GRCm39)	missense	probably damaging	1.00
R3953:Ank2	UTSW	3	126,781,809 (GRCm39)	missense	probably damaging	1.00
R3963:Ank2	UTSW	3	126,728,245 (GRCm39)	missense	probably benign
R4367:Ank2	UTSW	3	126,739,798 (GRCm39)	missense	probably benign
R4414:Ank2	UTSW	3	127,019,411 (GRCm39)	critical splice donor site	probably null
R4432:Ank2	UTSW	3	126,741,455 (GRCm39)	intron	probably benign
R4433:Ank2	UTSW	3	126,741,455 (GRCm39)	intron	probably benign
R4579:Ank2	UTSW	3	126,752,612 (GRCm39)	missense	probably damaging	1.00
R4597:Ank2	UTSW	3	126,781,800 (GRCm39)	missense	probably damaging	1.00
R4603:Ank2	UTSW	3	126,825,665 (GRCm39)	missense	probably benign	0.00
R4729:Ank2	UTSW	3	126,770,545 (GRCm39)	nonsense	probably null
R4815:Ank2	UTSW	3	126,730,410 (GRCm39)	missense	probably benign
R4826:Ank2	UTSW	3	126,749,650 (GRCm39)	missense	probably benign	0.35
R4871:Ank2	UTSW	3	126,753,444 (GRCm39)	missense	probably damaging	1.00
R4880:Ank2	UTSW	3	126,840,475 (GRCm39)	splice site	probably null
R4915:Ank2	UTSW	3	126,736,320 (GRCm39)	intron	probably benign
R4935:Ank2	UTSW	3	126,749,713 (GRCm39)	missense	probably damaging	1.00
R4936:Ank2	UTSW	3	126,748,688 (GRCm39)	missense	possibly damaging	0.94
R4937:Ank2	UTSW	3	126,756,050 (GRCm39)	missense	probably damaging	1.00
R4946:Ank2	UTSW	3	126,735,589 (GRCm39)	intron	probably benign
R4963:Ank2	UTSW	3	126,825,745 (GRCm39)	missense	probably benign	0.01
R4989:Ank2	UTSW	3	126,757,094 (GRCm39)	missense	possibly damaging	0.94
R5023:Ank2	UTSW	3	126,735,520 (GRCm39)	intron	probably benign
R5060:Ank2	UTSW	3	126,739,570 (GRCm39)	intron	probably benign
R5078:Ank2	UTSW	3	126,736,002 (GRCm39)	intron	probably benign
R5086:Ank2	UTSW	3	126,740,997 (GRCm39)	intron	probably benign
R5134:Ank2	UTSW	3	126,757,094 (GRCm39)	missense	possibly damaging	0.94
R5148:Ank2	UTSW	3	126,819,285 (GRCm39)	splice site	probably null
R5175:Ank2	UTSW	3	126,797,673 (GRCm39)	missense	probably damaging	1.00
R5275:Ank2	UTSW	3	126,825,832 (GRCm39)	missense	probably damaging	1.00
R5295:Ank2	UTSW	3	126,825,832 (GRCm39)	missense	probably damaging	1.00
R5303:Ank2	UTSW	3	126,739,453 (GRCm39)	intron	probably benign
R5309:Ank2	UTSW	3	126,753,417 (GRCm39)	missense	probably damaging	0.99
R5312:Ank2	UTSW	3	126,753,417 (GRCm39)	missense	probably damaging	0.99
R5352:Ank2	UTSW	3	127,292,640 (GRCm39)	utr 5 prime	probably benign
R5355:Ank2	UTSW	3	126,737,698 (GRCm39)	intron	probably benign
R5386:Ank2	UTSW	3	126,775,582 (GRCm39)	missense	probably benign	0.01
R5396:Ank2	UTSW	3	126,746,875 (GRCm39)	missense	probably damaging	1.00
R5518:Ank2	UTSW	3	126,753,348 (GRCm39)	missense	probably damaging	0.98
R5534:Ank2	UTSW	3	126,740,947 (GRCm39)	intron	probably benign
R5554:Ank2	UTSW	3	126,792,622 (GRCm39)	missense	possibly damaging	0.78
R5582:Ank2	UTSW	3	126,739,954 (GRCm39)	intron	probably benign
R5747:Ank2	UTSW	3	126,735,400 (GRCm39)	intron	probably benign
R5794:Ank2	UTSW	3	126,723,669 (GRCm39)	missense	probably benign	0.00
R5831:Ank2	UTSW	3	127,132,808 (GRCm39)	start gained	probably benign
R5925:Ank2	UTSW	3	126,726,612 (GRCm39)	missense	probably benign	0.18
R5954:Ank2	UTSW	3	126,791,510 (GRCm39)	missense	probably benign	0.34
R5956:Ank2	UTSW	3	126,736,337 (GRCm39)	intron	probably benign
R5986:Ank2	UTSW	3	126,806,335 (GRCm39)	missense	possibly damaging	0.94
R5992:Ank2	UTSW	3	126,753,300 (GRCm39)	critical splice donor site	probably null
R6020:Ank2	UTSW	3	126,740,470 (GRCm39)	intron	probably benign
R6027:Ank2	UTSW	3	126,791,528 (GRCm39)	missense	possibly damaging	0.92
R6049:Ank2	UTSW	3	126,736,669 (GRCm39)	missense	possibly damaging	0.95
R6060:Ank2	UTSW	3	126,749,601 (GRCm39)	missense	probably damaging	1.00
R6114:Ank2	UTSW	3	126,804,700 (GRCm39)	missense	probably damaging	1.00
R6124:Ank2	UTSW	3	127,041,800 (GRCm39)	missense	probably benign	0.31
R6156:Ank2	UTSW	3	126,737,886 (GRCm39)	missense	probably damaging	1.00
R6173:Ank2	UTSW	3	126,846,395 (GRCm39)	missense	probably damaging	1.00
R6176:Ank2	UTSW	3	126,739,120 (GRCm39)	missense	probably benign	0.05
R6184:Ank2	UTSW	3	126,756,047 (GRCm39)	missense	probably damaging	1.00
R6199:Ank2	UTSW	3	126,797,655 (GRCm39)	missense	probably damaging	1.00
R6241:Ank2	UTSW	3	126,846,397 (GRCm39)	missense	probably damaging	1.00
R6254:Ank2	UTSW	3	126,735,453 (GRCm39)	intron	probably benign
R6259:Ank2	UTSW	3	126,810,635 (GRCm39)	missense	probably benign	0.28
R6260:Ank2	UTSW	3	126,737,206 (GRCm39)	missense	probably benign
R6321:Ank2	UTSW	3	126,740,587 (GRCm39)	intron	probably benign
R6393:Ank2	UTSW	3	126,723,406 (GRCm39)	missense	probably damaging	1.00
R6406:Ank2	UTSW	3	126,825,874 (GRCm39)	missense	probably damaging	1.00
R6544:Ank2	UTSW	3	126,726,871 (GRCm39)	missense	probably damaging	0.99
R6583:Ank2	UTSW	3	126,810,613 (GRCm39)	missense	probably damaging	1.00
R6739:Ank2	UTSW	3	126,873,643 (GRCm39)	missense	probably damaging	1.00
R6754:Ank2	UTSW	3	126,890,488 (GRCm39)	intron	probably benign
R6786:Ank2	UTSW	3	126,752,581 (GRCm39)	missense	probably damaging	0.99
R6798:Ank2	UTSW	3	126,737,913 (GRCm39)	intron	probably benign
R6882:Ank2	UTSW	3	126,739,406 (GRCm39)	intron	probably benign
R6940:Ank2	UTSW	3	126,735,621 (GRCm39)	intron	probably benign
R6949:Ank2	UTSW	3	126,804,533 (GRCm39)	missense	probably benign	0.00
R7001:Ank2	UTSW	3	126,871,230 (GRCm39)	missense	probably damaging	1.00
R7033:Ank2	UTSW	3	126,738,499 (GRCm39)	nonsense	probably null
R7036:Ank2	UTSW	3	126,740,041 (GRCm39)	intron	probably benign
R7045:Ank2	UTSW	3	126,806,393 (GRCm39)	missense	probably damaging	1.00
R7054:Ank2	UTSW	3	126,736,952 (GRCm39)	intron	probably benign
R7069:Ank2	UTSW	3	126,739,947 (GRCm39)	intron	probably benign
R7091:Ank2	UTSW	3	126,817,000 (GRCm39)	missense	probably damaging	0.98
R7107:Ank2	UTSW	3	126,797,631 (GRCm39)	missense	probably damaging	1.00
R7175:Ank2	UTSW	3	126,740,590 (GRCm39)	missense	unknown
R7191:Ank2	UTSW	3	126,740,041 (GRCm39)	missense	unknown
R7272:Ank2	UTSW	3	126,736,782 (GRCm39)	missense	unknown
R7381:Ank2	UTSW	3	126,730,277 (GRCm39)	missense	possibly damaging	0.46
R7394:Ank2	UTSW	3	126,730,302 (GRCm39)	missense	possibly damaging	0.77
R7462:Ank2	UTSW	3	126,736,683 (GRCm39)	missense	unknown
R7490:Ank2	UTSW	3	126,752,538 (GRCm39)	missense	probably damaging	0.99
R7514:Ank2	UTSW	3	126,819,252 (GRCm39)	missense	probably benign	0.06
R7534:Ank2	UTSW	3	126,727,982 (GRCm39)	splice site	probably null
R7540:Ank2	UTSW	3	126,781,808 (GRCm39)	missense	possibly damaging	0.94
R7547:Ank2	UTSW	3	126,738,852 (GRCm39)	missense	unknown
R7579:Ank2	UTSW	3	126,740,047 (GRCm39)	missense	unknown
R7584:Ank2	UTSW	3	126,739,777 (GRCm39)	nonsense	probably null
R7625:Ank2	UTSW	3	126,846,449 (GRCm39)	missense	probably damaging	1.00
R7698:Ank2	UTSW	3	126,825,860 (GRCm39)	missense	probably benign	0.35
R7716:Ank2	UTSW	3	126,736,815 (GRCm39)	missense	unknown
R7718:Ank2	UTSW	3	126,758,662 (GRCm39)	missense	possibly damaging	0.88
R7722:Ank2	UTSW	3	126,822,951 (GRCm39)	missense	probably benign	0.01
R7738:Ank2	UTSW	3	126,741,271 (GRCm39)	missense
R7977:Ank2	UTSW	3	126,739,356 (GRCm39)	missense	unknown
R7987:Ank2	UTSW	3	126,739,356 (GRCm39)	missense	unknown
R8007:Ank2	UTSW	3	126,730,096 (GRCm39)	intron	probably benign
R8150:Ank2	UTSW	3	126,741,162 (GRCm39)	missense
R8161:Ank2	UTSW	3	126,825,778 (GRCm39)	missense
R8196:Ank2	UTSW	3	126,723,532 (GRCm39)	missense	probably damaging	0.99
R8248:Ank2	UTSW	3	126,731,434 (GRCm39)	missense	possibly damaging	0.78
R8255:Ank2	UTSW	3	126,740,398 (GRCm39)	missense	unknown
R8279:Ank2	UTSW	3	126,726,820 (GRCm39)	missense	probably benign	0.04
R8300:Ank2	UTSW	3	126,804,555 (GRCm39)	missense
R8716:Ank2	UTSW	3	126,736,488 (GRCm39)	nonsense	probably null
R8724:Ank2	UTSW	3	126,737,405 (GRCm39)	missense	unknown
R8765:Ank2	UTSW	3	126,850,731 (GRCm39)	missense	possibly damaging	0.94
R8779:Ank2	UTSW	3	126,758,751 (GRCm39)	missense	probably damaging	0.99
R8783:Ank2	UTSW	3	126,846,455 (GRCm39)	missense	probably damaging	1.00
R8785:Ank2	UTSW	3	126,791,570 (GRCm39)	missense	probably damaging	1.00
R8826:Ank2	UTSW	3	126,740,951 (GRCm39)	missense	unknown
R8872:Ank2	UTSW	3	126,791,525 (GRCm39)	missense	possibly damaging	0.88
R8903:Ank2	UTSW	3	126,840,431 (GRCm39)	missense	probably damaging	1.00
R8906:Ank2	UTSW	3	126,726,720 (GRCm39)	missense	probably benign	0.00
R8918:Ank2	UTSW	3	126,737,380 (GRCm39)	missense	unknown
R8947:Ank2	UTSW	3	126,736,396 (GRCm39)	intron	probably benign
R8977:Ank2	UTSW	3	126,738,575 (GRCm39)	missense	unknown
R8990:Ank2	UTSW	3	126,841,829 (GRCm39)	critical splice donor site	probably null
R8994:Ank2	UTSW	3	126,723,471 (GRCm39)	missense	probably benign	0.19
R9009:Ank2	UTSW	3	126,728,025 (GRCm39)	unclassified	probably benign
R9123:Ank2	UTSW	3	126,733,744 (GRCm39)	missense	probably damaging	1.00
R9125:Ank2	UTSW	3	126,733,744 (GRCm39)	missense	probably damaging	1.00
R9130:Ank2	UTSW	3	126,810,565 (GRCm39)	missense
R9175:Ank2	UTSW	3	126,722,402 (GRCm39)	missense	possibly damaging	0.52
R9220:Ank2	UTSW	3	126,737,086 (GRCm39)	missense	unknown
R9225:Ank2	UTSW	3	126,736,111 (GRCm39)	missense	unknown
R9286:Ank2	UTSW	3	126,846,381 (GRCm39)	missense	probably damaging	0.99
R9325:Ank2	UTSW	3	126,775,504 (GRCm39)	missense	probably damaging	0.98
R9367:Ank2	UTSW	3	126,738,678 (GRCm39)	missense	unknown
R9385:Ank2	UTSW	3	126,753,366 (GRCm39)	missense	probably benign	0.00
R9391:Ank2	UTSW	3	126,731,394 (GRCm39)	missense	probably damaging	0.99
R9422:Ank2	UTSW	3	126,890,505 (GRCm39)	missense	unknown
R9536:Ank2	UTSW	3	126,736,031 (GRCm39)	missense	unknown
R9647:Ank2	UTSW	3	126,792,623 (GRCm39)	missense	possibly damaging	0.93
R9650:Ank2	UTSW	3	126,735,829 (GRCm39)	missense	unknown
R9666:Ank2	UTSW	3	126,726,838 (GRCm39)	nonsense	probably null
R9686:Ank2	UTSW	3	126,740,550 (GRCm39)	missense	unknown
R9730:Ank2	UTSW	3	127,019,493 (GRCm39)	missense
R9738:Ank2	UTSW	3	126,737,121 (GRCm39)	missense	unknown
R9743:Ank2	UTSW	3	126,733,794 (GRCm39)	missense	possibly damaging	0.81
R9747:Ank2	UTSW	3	126,752,667 (GRCm39)	missense	probably damaging	1.00
R9800:Ank2	UTSW	3	126,740,149 (GRCm39)	missense	unknown
R9803:Ank2	UTSW	3	126,752,726 (GRCm39)	missense	possibly damaging	0.64
RF020:Ank2	UTSW	3	126,739,125 (GRCm39)	missense	unknown
Z1088:Ank2	UTSW	3	126,823,158 (GRCm39)	missense	possibly damaging	0.45
Z1177:Ank2	UTSW	3	126,738,006 (GRCm39)	missense	unknown
Z1187:Ank2	UTSW	3	126,749,601 (GRCm39)	missense	probably damaging	1.00
Z1190:Ank2	UTSW	3	126,749,601 (GRCm39)	missense	probably damaging	1.00
Z1192:Ank2	UTSW	3	126,749,601 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTCAGGGAGAGAAATGTATCTGC -3'
(R):5'- TATTGGGACATGGCTACTGGATC -3'

Sequencing Primer
(F):5'- CAAATGTCTGAGTTATTGGTGCATC -3'
(R):5'- ATGGCTACTGGATCCAGAACTCTG -3'

Posted On

2019-05-13