Mutagenetix > Incidental Mutation

Incidental Mutation 'R7048:Taar1'

547447

Institutional Source

Beutler Lab

Gene Symbol

Taar1

Ensembl Gene

ENSMUSG00000056379

Gene Name

trace amine-associated receptor 1

Synonyms

Trar1, Tar1

MMRRC Submission

045146-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.114) question?

Stock #

R7048 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23796304-23797302 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23796722 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 140 (L140P)

Ref Sequence

ENSEMBL: ENSMUSP00000049527 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051532]

AlphaFold

Q923Y8

Predicted Effect

probably benign
Transcript: ENSMUST00000051532
AA Change: L140P

PolyPhen 2 Score 0.403 (Sensitivity: 0.89; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000049527
Gene: ENSMUSG00000056379
AA Change: L140P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	316	1.4e-11	PFAM
Pfam:7tm_1	39	301	2.4e-62	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

97% (66/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a G-protein coupled receptor activated by trace amines. The encoded protein responds little or not at all to dopamine, serotonin, epinephrine, or histamine, but responds well to beta-phenylethylamine, p-tyramine, octopamine, and tryptamine. While primarily functioning in neurologic systems, there is evidence that this gene is involved in blood cell and immunologic functions as well. This gene is thought to be intronless. [provided by RefSeq, Nov 2015]
PHENOTYPE: Mice homozygous for a null mutation display decreased prepulse inhibition and increased sensitivity to amphetamines. Mice homozygous for another knock-out allele exhibit increased sensitivity to MDMA-induced hyperthermia, brain dopamine and serotonin levels, and induced hyperactivity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl2	T	C	3: 148,552,565 (GRCm39)	D629G	probably damaging	Het
Akap11	T	A	14: 78,749,954 (GRCm39)	Q811L		Het
Ank2	T	A	3: 126,819,267 (GRCm39)	Q468L	probably benign	Het
Ano3	A	T	2: 110,513,116 (GRCm39)	Y626*	probably null	Het
Ap1m1	A	G	8: 73,003,642 (GRCm39)	N114S	probably damaging	Het
Asb3	T	A	11: 31,051,121 (GRCm39)	I525N	probably damaging	Het
Atg4a-ps	A	G	3: 103,552,672 (GRCm39)	I223T	probably damaging	Het
B3gnt7	T	C	1: 86,233,308 (GRCm39)	Y68H	probably benign	Het
Bbs5	T	C	2: 69,484,705 (GRCm39)	I125T	probably benign	Het
Cd180	A	G	13: 102,841,431 (GRCm39)	N159S	probably damaging	Het
Cd37	T	C	7: 44,887,888 (GRCm39)		probably benign	Het
Cdk14	T	A	5: 5,143,005 (GRCm39)	Q242L	probably damaging	Het
Clcn1	T	C	6: 42,284,477 (GRCm39)	V605A	probably damaging	Het
Cped1	G	A	6: 22,119,469 (GRCm39)	M309I	probably benign	Het
Cyp2r1	A	T	7: 114,151,971 (GRCm39)	Y120N	probably damaging	Het
Ddx3y	A	T	Y: 1,279,491 (GRCm39)	S124R	probably benign	Het
Dlec1	A	T	9: 118,972,472 (GRCm39)		probably null	Het
Dnah17	T	C	11: 117,936,944 (GRCm39)	E3420G	possibly damaging	Het
Dnajc13	C	T	9: 104,080,613 (GRCm39)		probably null	Het
Dusp23	A	T	1: 172,459,253 (GRCm39)	Y136*	probably null	Het
Eif4b	A	G	15: 102,001,571 (GRCm39)		probably benign	Het
F13a1	A	G	13: 37,082,117 (GRCm39)	V529A	probably benign	Het
Fhl2	A	G	1: 43,162,808 (GRCm39)	Y236H	probably damaging	Het
Gm826	C	T	2: 160,169,026 (GRCm39)	W94*	probably null	Het
Gpsm2	C	T	3: 108,610,361 (GRCm39)	R33H	probably damaging	Het
Hmcn1	T	C	1: 150,475,404 (GRCm39)		probably null	Het
Ifit1bl2	T	C	19: 34,596,551 (GRCm39)	D355G	probably benign	Het
Itga8	A	G	2: 12,115,895 (GRCm39)	V77A	probably damaging	Het
Kcmf1	C	T	6: 72,826,450 (GRCm39)	R40K	probably damaging	Het
Kdm7a	T	C	6: 39,145,982 (GRCm39)	E315G	probably damaging	Het
Kmt2b	C	T	7: 30,268,731 (GRCm39)	G2666D	probably damaging	Het
Lrrtm1	A	G	6: 77,221,152 (GRCm39)	N203S	probably damaging	Het
Mdn1	A	G	4: 32,767,969 (GRCm39)	N5301D	probably benign	Het
Mest	T	G	6: 30,742,723 (GRCm39)	H108Q	probably damaging	Het
Mettl25b	A	G	3: 87,837,167 (GRCm39)	I36T	probably damaging	Het
Moxd1	C	A	10: 24,157,374 (GRCm39)	D335E	probably damaging	Het
Ncf2	A	C	1: 152,683,921 (GRCm39)	N47H	probably benign	Het
Npc1	A	G	18: 12,337,822 (GRCm39)		probably null	Het
Or14j2	A	T	17: 37,886,114 (GRCm39)	S67T	probably damaging	Het
Pcid2	A	G	8: 13,128,243 (GRCm39)	V386A	probably benign	Het
Phactr2	T	A	10: 13,121,168 (GRCm39)	T444S	probably benign	Het
Plekha7	T	C	7: 115,747,559 (GRCm39)	N710D	probably benign	Het
Ppp1r16b	C	T	2: 158,599,174 (GRCm39)	T382I	probably benign	Het
Pramel15	T	C	4: 144,103,754 (GRCm39)	D124G	probably benign	Het
Ptprz1	A	T	6: 22,961,622 (GRCm39)	Y111F	probably benign	Het
Rbbp8	A	G	18: 11,865,277 (GRCm39)	E722G	possibly damaging	Het
Rimbp3	A	G	16: 17,028,190 (GRCm39)	D538G	probably benign	Het
Rims1	A	G	1: 22,511,901 (GRCm39)	S551P	probably damaging	Het
Rsrc1	A	G	3: 67,088,164 (GRCm39)	D166G	probably damaging	Het
Selenon	T	C	4: 134,270,154 (GRCm39)	N350S	probably benign	Het
Sh3gl2	T	A	4: 85,295,802 (GRCm39)	L168H	probably damaging	Het
Smc3	T	C	19: 53,617,682 (GRCm39)	Y560H	probably benign	Het
Syce1	C	T	7: 140,359,281 (GRCm39)	D147N	possibly damaging	Het
Syt10	A	C	15: 89,675,008 (GRCm39)	V446G	probably damaging	Het
Tfpi2	C	A	6: 3,968,032 (GRCm39)	C36F	probably damaging	Het
Thoc5	G	A	11: 4,876,237 (GRCm39)		probably null	Het
Tnrc6c	T	A	11: 117,612,800 (GRCm39)	N319K	probably benign	Het
Trhr2	C	T	8: 123,085,418 (GRCm39)	D189N	probably damaging	Het
Trim42	A	T	9: 97,245,474 (GRCm39)	F442Y	probably damaging	Het
Ubap2	G	A	4: 41,196,033 (GRCm39)	T949I	possibly damaging	Het
Ugt1a8	T	C	1: 88,016,024 (GRCm39)	F146L	probably benign	Het
Vmn2r80	C	A	10: 79,030,153 (GRCm39)	Q660K	probably damaging	Het
Vstm2b	C	T	7: 40,578,800 (GRCm39)	T258I	possibly damaging	Het
Washc2	T	C	6: 116,197,544 (GRCm39)	L259P	possibly damaging	Het
Zscan18	A	T	7: 12,508,671 (GRCm39)		probably benign	Het
Zzef1	T	G	11: 72,757,525 (GRCm39)	Y1193*	probably null	Het

Other mutations in Taar1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00782:Taar1	APN	10	23,796,344 (GRCm39)	missense	probably benign	0.00
R0022:Taar1	UTSW	10	23,796,625 (GRCm39)	missense	probably benign	0.45
R0126:Taar1	UTSW	10	23,796,445 (GRCm39)	missense	probably benign	0.00
R0442:Taar1	UTSW	10	23,796,380 (GRCm39)	missense	possibly damaging	0.80
R0720:Taar1	UTSW	10	23,796,971 (GRCm39)	missense	probably damaging	1.00
R0931:Taar1	UTSW	10	23,797,181 (GRCm39)	missense	probably damaging	1.00
R1270:Taar1	UTSW	10	23,796,431 (GRCm39)	missense	probably damaging	1.00
R1834:Taar1	UTSW	10	23,797,087 (GRCm39)	missense	probably benign	0.04
R2137:Taar1	UTSW	10	23,797,168 (GRCm39)	missense	probably benign
R3765:Taar1	UTSW	10	23,797,205 (GRCm39)	missense	probably damaging	1.00
R3873:Taar1	UTSW	10	23,796,482 (GRCm39)	missense	probably damaging	1.00
R5334:Taar1	UTSW	10	23,796,443 (GRCm39)	missense	probably damaging	1.00
R5418:Taar1	UTSW	10	23,797,214 (GRCm39)	missense	possibly damaging	0.91
R5578:Taar1	UTSW	10	23,796,718 (GRCm39)	missense	possibly damaging	0.71
R7096:Taar1	UTSW	10	23,796,809 (GRCm39)	missense	possibly damaging	0.48
R7163:Taar1	UTSW	10	23,796,918 (GRCm39)	missense	probably benign	0.38
R7707:Taar1	UTSW	10	23,797,135 (GRCm39)	missense	possibly damaging	0.69
R7854:Taar1	UTSW	10	23,796,680 (GRCm39)	missense	probably benign	0.00
R8036:Taar1	UTSW	10	23,797,033 (GRCm39)	missense	probably benign	0.01
R8442:Taar1	UTSW	10	23,796,522 (GRCm39)	nonsense	probably null
R8855:Taar1	UTSW	10	23,796,976 (GRCm39)	missense	possibly damaging	0.50
R9777:Taar1	UTSW	10	23,796,676 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GGCCATTGTCGACTTTCTGC -3'
(R):5'- AGGAAGTCATGAACGCCAGTAC -3'

Sequencing Primer
(F):5'- GCTGTCTGATAATGCCCTGCAG -3'
(R):5'- CCCCAGATACTTTACTAAAGAAGGG -3'

Posted On

2019-05-13