Mutagenetix > Incidental Mutation

Incidental Mutation 'R7055:Trim25'

547869

Institutional Source

Beutler Lab

Gene Symbol

Trim25

Ensembl Gene

ENSMUSG00000000275

Gene Name

tripartite motif-containing 25

Synonyms

Zfp147, estrogen-responsive finger protein

MMRRC Submission

045152-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7055 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

88890202-88911119 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88890750 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 146 (S146P)

Ref Sequence

ENSEMBL: ENSMUSP00000103528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000284] [ENSMUST00000100627] [ENSMUST00000107896]

AlphaFold

Q61510

PDB Structure

PRY-SPRY domain of Trim25 [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000000284
AA Change: S146P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000000284
Gene: ENSMUSG00000000275
AA Change: S146P

Domain	Start	End	E-Value	Type
RING	13	53	8.04e-9	SMART
Blast:SPEC	189	288	5e-34	BLAST
PDB:4LTB\|B	189	380	7e-69	PDB
PRY	453	505	3.44e-17	SMART
SPRY	506	626	9.62e-31	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000100627
AA Change: S146P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000098192
Gene: ENSMUSG00000000275
AA Change: S146P

Domain	Start	End	E-Value	Type
RING	13	53	8.04e-9	SMART
Blast:BBOX	151	186	3e-8	BLAST
Blast:SPEC	189	288	2e-37	BLAST
PDB:4LTB\|B	189	380	2e-71	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000107896
AA Change: S146P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103528
Gene: ENSMUSG00000000275
AA Change: S146P

Domain	Start	End	E-Value	Type
RING	13	53	8.04e-9	SMART
Blast:SPEC	189	288	3e-34	BLAST
PDB:4LTB\|B	189	380	8e-69	PDB
low complexity region	382	393	N/A	INTRINSIC
PRY	461	513	3.44e-17	SMART
SPRY	514	634	9.62e-31	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Engineered mutations result in a compromised response to estrogen resulting in functional but small uteri. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	T	C	10: 87,062,083 (GRCm39)	S178P	probably damaging	Het
2610008E11Rik	C	T	10: 78,903,681 (GRCm39)	E212K	probably damaging	Het
Abcc4	A	T	14: 118,832,197 (GRCm39)	L736*	probably null	Het
Acmsd	A	T	1: 127,681,570 (GRCm39)	M178L	probably benign	Het
Adpgk	T	A	9: 59,220,476 (GRCm39)	M266K	possibly damaging	Het
Aldh1b1	G	A	4: 45,802,909 (GRCm39)	R149H	possibly damaging	Het
Aox1	A	G	1: 58,338,927 (GRCm39)	T307A	probably benign	Het
C1galt1	T	C	6: 7,866,585 (GRCm39)	Y144H	probably damaging	Het
Cabin1	G	C	10: 75,579,117 (GRCm39)	Q440E	probably benign	Het
Casq2	A	G	3: 102,049,561 (GRCm39)	S231G	probably damaging	Het
Catsper2	TAGGATGGCTTTTCTCAGGATAGCTTTTCTCAGGATGGCTTTTCTCAGGATAGCTTTTCTCAGGATGGCTTTTCTCAGGATAGCTTTTCT	TAGGATGGCTTTTCTCAGGATAGCTTTTCTCAGGATGGCTTTTCTCAGGATAGCTTTTCT	2: 121,228,053 (GRCm39)		probably benign	Het
Cdan1	A	G	2: 120,558,342 (GRCm39)	I490T	probably damaging	Het
Cep170b	T	A	12: 112,702,149 (GRCm39)	V314E	probably damaging	Het
Col4a4	T	C	1: 82,496,757 (GRCm39)	E413G	unknown	Het
Cyp3a25	A	C	5: 145,929,801 (GRCm39)	F189L	probably benign	Het
Dido1	T	A	2: 180,303,002 (GRCm39)	H1634L	probably benign	Het
Dnaja2	A	C	8: 86,275,303 (GRCm39)	V156G	probably benign	Het
Dpy19l4	T	C	4: 11,290,291 (GRCm39)		probably null	Het
Eomes	A	T	9: 118,309,567 (GRCm39)	N240I	possibly damaging	Het
Fads6	A	G	11: 115,176,229 (GRCm39)	F199L	probably benign	Het
Fbrs	T	C	7: 127,086,956 (GRCm39)		probably null	Het
Fndc4	T	C	5: 31,451,522 (GRCm39)	E153G	probably benign	Het
Fv1	TTCTCTCTCTCTCTC	TTCTCTCTCTCTCTCTC	4: 147,954,775 (GRCm39)		probably null	Het
Fzr1	T	C	10: 81,206,057 (GRCm39)	Y210C	probably damaging	Het
Gjc2	T	A	11: 59,067,856 (GRCm39)	M209L	unknown	Het
Gpr33	A	G	12: 52,071,036 (GRCm39)	M1T	probably null	Het
Gtf2a1	A	T	12: 91,553,523 (GRCm39)	I28N	possibly damaging	Het
Igf2r	A	C	17: 12,923,210 (GRCm39)	Y1200D	probably damaging	Het
Ivd	C	T	2: 118,703,730 (GRCm39)	T212I	probably damaging	Het
Jag1	A	G	2: 136,957,409 (GRCm39)	V101A	probably benign	Het
Kansl3	A	T	1: 36,404,701 (GRCm39)	V83D	possibly damaging	Het
Krt81	T	A	15: 101,359,006 (GRCm39)	I249F	probably benign	Het
Krtap21-1	G	A	16: 89,200,591 (GRCm39)	S17L	unknown	Het
Macf1	A	T	4: 123,302,989 (GRCm39)	H504Q	probably benign	Het
Map2	A	G	1: 66,455,983 (GRCm39)	T1499A	probably damaging	Het
Map3k9	T	C	12: 81,770,982 (GRCm39)	T892A	probably damaging	Het
Mcl1	G	A	3: 95,567,110 (GRCm39)	V178I	probably benign	Het
Mrs2	T	A	13: 25,188,937 (GRCm39)	M126L	probably benign	Het
Msantd1	C	A	5: 35,075,005 (GRCm39)	N9K	probably benign	Het
Myh9	C	T	15: 77,659,398 (GRCm39)	R116H	probably damaging	Het
Nfib	A	T	4: 82,248,662 (GRCm39)	D308E	probably benign	Het
Nme2	T	A	11: 93,846,416 (GRCm39)	I11F	probably damaging	Het
Nmnat3	A	G	9: 98,292,286 (GRCm39)	D111G	probably benign	Het
Or5d37	T	A	2: 87,924,045 (GRCm39)	L78F	probably damaging	Het
Papss2	T	C	19: 32,641,827 (GRCm39)	W501R	probably damaging	Het
Parn	A	G	16: 13,443,998 (GRCm39)	I384T	possibly damaging	Het
Pcdhb18	A	G	18: 37,623,864 (GRCm39)	D398G	possibly damaging	Het
Pdcl2	T	C	5: 76,465,771 (GRCm39)	N102D	probably benign	Het
Pdzrn3	C	A	6: 101,128,735 (GRCm39)	E644*	probably null	Het
Pi4ka	T	A	16: 17,134,879 (GRCm39)		probably benign	Het
Polg2	A	G	11: 106,668,040 (GRCm39)	F216L	probably damaging	Het
Prkcsh	A	G	9: 21,924,457 (GRCm39)	*522W	probably null	Het
Prkcz	G	T	4: 155,374,091 (GRCm39)	D108E	probably benign	Het
Pros1	T	C	16: 62,748,465 (GRCm39)	V646A	possibly damaging	Het
Ptprc	A	G	1: 138,017,309 (GRCm39)	I483T	probably damaging	Het
Rabep2	T	C	7: 126,044,485 (GRCm39)	I527T	possibly damaging	Het
Rad50	T	C	11: 53,578,929 (GRCm39)	K543R	probably benign	Het
Samd4b	A	G	7: 28,103,458 (GRCm39)	I553T	probably benign	Het
Sbpl	A	T	17: 24,172,276 (GRCm39)	N214K	unknown	Het
Scgb2b11	C	T	7: 31,909,907 (GRCm39)	E60K	possibly damaging	Het
Sgk3	G	A	1: 9,956,284 (GRCm39)	E331K	probably damaging	Het
Snx6	A	T	12: 54,830,864 (GRCm39)	L32Q	probably damaging	Het
Srgap3	T	A	6: 112,723,924 (GRCm39)	Q512L	probably damaging	Het
Srsf12	A	G	4: 33,226,157 (GRCm39)	D135G	probably damaging	Het
Steap4	A	G	5: 8,026,858 (GRCm39)	T274A	probably damaging	Het
Svep1	A	T	4: 58,064,275 (GRCm39)	V3236D	probably benign	Het
Svep1	A	T	4: 58,120,642 (GRCm39)	F797Y	probably benign	Het
Tmem116	T	C	5: 121,605,987 (GRCm39)	L113P	probably damaging	Het
Tnfrsf1b	A	G	4: 144,951,457 (GRCm39)	V161A	probably damaging	Het
Tnpo1	G	T	13: 98,991,987 (GRCm39)	Q622K	possibly damaging	Het
Top1mt	A	G	15: 75,550,523 (GRCm39)	V28A	probably benign	Het
Tuba3b	A	G	6: 145,566,935 (GRCm39)	D392G	possibly damaging	Het
Utrn	C	T	10: 12,623,665 (GRCm39)	R191Q	probably benign	Het
Vmn2r111	T	C	17: 22,778,032 (GRCm39)	N549S	possibly damaging	Het
Wdfy2	T	A	14: 63,137,748 (GRCm39)	S84T	probably benign	Het
Zfp873	T	A	10: 81,895,832 (GRCm39)	F225I	probably damaging	Het

Other mutations in Trim25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01707:Trim25	APN	11	88,890,517 (GRCm39)	missense	probably damaging	0.96
IGL02398:Trim25	APN	11	88,890,630 (GRCm39)	missense	probably damaging	1.00
IGL03150:Trim25	APN	11	88,890,831 (GRCm39)	missense	probably damaging	1.00
R0003:Trim25	UTSW	11	88,906,598 (GRCm39)	missense	probably benign	0.01
R0184:Trim25	UTSW	11	88,890,466 (GRCm39)	missense	probably damaging	1.00
R0707:Trim25	UTSW	11	88,890,564 (GRCm39)	missense	probably benign	0.03
R1855:Trim25	UTSW	11	88,906,407 (GRCm39)	missense	probably benign	0.04
R1936:Trim25	UTSW	11	88,895,576 (GRCm39)	missense	probably benign	0.03
R2229:Trim25	UTSW	11	88,907,447 (GRCm39)	missense	probably damaging	0.97
R3401:Trim25	UTSW	11	88,901,707 (GRCm39)	missense	probably benign
R5159:Trim25	UTSW	11	88,890,358 (GRCm39)	missense	probably benign	0.20
R5378:Trim25	UTSW	11	88,900,093 (GRCm39)	missense	probably damaging	1.00
R6149:Trim25	UTSW	11	88,906,362 (GRCm39)	missense	probably benign	0.00
R6867:Trim25	UTSW	11	88,901,713 (GRCm39)	missense	probably benign	0.00
R6996:Trim25	UTSW	11	88,890,329 (GRCm39)	missense	probably benign	0.00
R7310:Trim25	UTSW	11	88,906,608 (GRCm39)	missense	probably benign	0.03
R7451:Trim25	UTSW	11	88,906,563 (GRCm39)	missense	possibly damaging	0.76
R7632:Trim25	UTSW	11	88,906,602 (GRCm39)	missense	probably null	0.91
R7767:Trim25	UTSW	11	88,899,943 (GRCm39)	critical splice acceptor site	probably null
R8132:Trim25	UTSW	11	88,907,432 (GRCm39)	missense	probably damaging	0.99
R8785:Trim25	UTSW	11	88,904,340 (GRCm39)	missense	probably benign	0.00
R8978:Trim25	UTSW	11	88,907,027 (GRCm39)	missense	probably benign	0.01
R9135:Trim25	UTSW	11	88,899,988 (GRCm39)	missense	probably benign
R9189:Trim25	UTSW	11	88,901,731 (GRCm39)	missense	probably benign	0.00
R9348:Trim25	UTSW	11	88,900,167 (GRCm39)	nonsense	probably null
R9667:Trim25	UTSW	11	88,907,188 (GRCm39)	missense	probably damaging	1.00
R9731:Trim25	UTSW	11	88,906,391 (GRCm39)	missense	probably benign	0.00
X0022:Trim25	UTSW	11	88,906,422 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- AGAACACGGTAATGTGCGCG -3'
(R):5'- AGCTGATCCAAGCTGTCCTC -3'

Sequencing Primer
(F):5'- AGTTCCTGCAGGCCGAG -3'
(R):5'- AGCTGATCCAAGCTGTCCTCTATAC -3'

Posted On

2019-05-13