Mutagenetix > Incidental Mutation

Incidental Mutation 'R7061:Zfp442'

548242

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

MMRRC Submission

045384-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7061 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

150249061-150293406 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 150249937 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 655 (I655T)

Ref Sequence

ENSEMBL: ENSMUSP00000105542 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916] [ENSMUST00000185796]

AlphaFold

A2AQA0

Predicted Effect

probably benign
Transcript: ENSMUST00000109916
AA Change: I655T

PolyPhen 2 Score 0.332 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130
AA Change: I655T

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000185796
AA Change: I598T

PolyPhen 2 Score 0.258 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130
AA Change: I598T

Domain	Start	End	E-Value	Type
KRAB	3	65	1.4e-21	SMART
ZnF_C2H2	158	180	3.4e-5	SMART
ZnF_C2H2	210	232	3.9e-5	SMART
ZnF_C2H2	238	260	1e-6	SMART
ZnF_C2H2	266	288	5.6e-6	SMART
ZnF_C2H2	294	316	1.8e-5	SMART
ZnF_C2H2	322	344	1.3e-5	SMART
ZnF_C2H2	350	372	6.7e-6	SMART
ZnF_C2H2	378	400	9.6e-5	SMART
ZnF_C2H2	406	428	6.9e-7	SMART
ZnF_C2H2	434	456	7.7e-6	SMART
ZnF_C2H2	462	484	1.9e-5	SMART
ZnF_C2H2	490	512	1.5e-5	SMART
ZnF_C2H2	518	540	2e-5	SMART
ZnF_C2H2	546	568	3.5e-6	SMART
ZnF_C2H2	574	597	1.3e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

99% (68/69)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aard	G	A	15: 51,903,617 (GRCm39)	M13I	probably benign	Het
Abcb5	A	C	12: 118,841,509 (GRCm39)	Y979D	probably damaging	Het
Adgrb1	G	C	15: 74,441,730 (GRCm39)	V4L	probably benign	Het
Ap3b2	C	T	7: 81,110,757 (GRCm39)	R1006Q	unknown	Het
Bpifa6	A	T	2: 153,834,236 (GRCm39)	T343S	probably benign	Het
Celsr3	T	A	9: 108,724,793 (GRCm39)	C2957*	probably null	Het
Chd6	G	A	2: 160,867,885 (GRCm39)	Q428*	probably null	Het
Col5a1	C	A	2: 27,915,690 (GRCm39)	C191*	probably null	Het
Cpox	A	G	16: 58,491,223 (GRCm39)	I145V	possibly damaging	Het
Csnk2a1	C	T	2: 152,116,091 (GRCm39)	R268C	probably benign	Het
Dclk2	A	G	3: 86,739,038 (GRCm39)		probably null	Het
Dennd5a	T	C	7: 109,504,386 (GRCm39)	E909G	probably benign	Het
Depdc1a	T	A	3: 159,228,489 (GRCm39)	S414T	possibly damaging	Het
Dock5	A	G	14: 68,007,703 (GRCm39)	F1502S	probably damaging	Het
Dop1b	G	T	16: 93,558,951 (GRCm39)	A448S	probably benign	Het
Dsg1c	C	A	18: 20,410,066 (GRCm39)	N511K	probably benign	Het
Epc2	G	T	2: 49,425,334 (GRCm39)	R108L	probably damaging	Het
Erp44	T	A	4: 48,219,375 (GRCm39)	I147F	probably benign	Het
Evc	T	C	5: 37,476,446 (GRCm39)	T368A	possibly damaging	Het
Fbxo41	G	T	6: 85,452,448 (GRCm39)	R738S	probably benign	Het
Fli1	T	C	9: 32,335,518 (GRCm39)	T305A	probably damaging	Het
Grk5	A	G	19: 61,034,530 (GRCm39)	T93A	probably benign	Het
Grm2	A	T	9: 106,528,424 (GRCm39)	N153K	probably damaging	Het
Helz	A	G	11: 107,540,003 (GRCm39)	T1007A	possibly damaging	Het
Helz2	A	G	2: 180,882,307 (GRCm39)	L162P	probably damaging	Het
Hmgcr	T	A	13: 96,802,656 (GRCm39)	Q81L	possibly damaging	Het
Hnrnpu	T	C	1: 178,163,691 (GRCm39)	K218E	unknown	Het
Hydin	A	T	8: 111,329,920 (GRCm39)	I4885F	possibly damaging	Het
Ibsp	A	G	5: 104,457,768 (GRCm39)		probably null	Het
Igfals	T	C	17: 25,099,281 (GRCm39)	L124P	probably damaging	Het
Il24	T	A	1: 130,811,108 (GRCm39)	H142L	possibly damaging	Het
Iqca1l	A	G	5: 24,750,063 (GRCm39)	M660T	probably benign	Het
Kcnh2	A	T	5: 24,536,920 (GRCm39)	H221Q	probably benign	Het
Man1a	A	G	10: 53,796,331 (GRCm39)	S454P	probably damaging	Het
Meiosin	A	T	7: 18,834,053 (GRCm39)		probably benign	Het
Mfsd4b1	C	T	10: 39,879,382 (GRCm39)	V172M	possibly damaging	Het
Mical2	T	A	7: 111,946,008 (GRCm39)	I763K	probably benign	Het
Mybpc3	A	G	2: 90,955,749 (GRCm39)	I594M	possibly damaging	Het
Neo1	T	A	9: 58,897,724 (GRCm39)	R77S	possibly damaging	Het
Nlrp6	A	G	7: 140,502,780 (GRCm39)	I265M	probably benign	Het
Or52n5	T	C	7: 104,587,883 (GRCm39)	L50P	probably damaging	Het
Or5bw2	A	G	7: 6,573,782 (GRCm39)	Y264C	probably damaging	Het
Or8b9	T	A	9: 37,766,942 (GRCm39)	V276D	possibly damaging	Het
Pcdhgb5	C	A	18: 37,864,976 (GRCm39)	P257Q	probably benign	Het
Phactr1	A	G	13: 43,286,457 (GRCm39)	D586G	probably damaging	Het
Pkn3	T	A	2: 29,973,548 (GRCm39)		probably null	Het
Prob1	A	T	18: 35,787,553 (GRCm39)	S234T	probably benign	Het
Rab14	A	T	2: 35,073,429 (GRCm39)	L131*	probably null	Het
Rab5c	G	A	11: 100,610,789 (GRCm39)	R40C	probably damaging	Het
Rhebl1	A	G	15: 98,777,164 (GRCm39)	L103P	probably damaging	Het
Rnf10	G	T	5: 115,395,149 (GRCm39)	F146L	probably damaging	Het
Rrbp1	T	C	2: 143,831,087 (GRCm39)	K360R	possibly damaging	Het
Slc6a9	C	T	4: 117,725,261 (GRCm39)	T575I	probably benign	Het
Smo	A	G	6: 29,760,229 (GRCm39)	H776R	probably damaging	Het
Speer1f	T	C	5: 11,469,071 (GRCm39)	V74A	possibly damaging	Het
Tns2	A	G	15: 102,012,914 (GRCm39)	M1V	probably null	Het
Ttn	C	A	2: 76,725,036 (GRCm39)		probably benign	Het
Ugt3a1	A	T	15: 9,306,240 (GRCm39)	M130L	probably benign	Het
Urb2	C	T	8: 124,755,036 (GRCm39)	H248Y	probably benign	Het
Vit	A	T	17: 78,932,585 (GRCm39)	N564I	probably damaging	Het
Vmn1r60	A	T	7: 5,547,310 (GRCm39)	Y263*	probably null	Het
Vmn2r16	A	T	5: 109,511,620 (GRCm39)	Y609F	probably damaging	Het
Xpo7	T	C	14: 70,908,512 (GRCm39)	I876V	probably benign	Het
Zfp574	T	A	7: 24,779,622 (GRCm39)	C215S	possibly damaging	Het
Zfp608	T	C	18: 55,121,069 (GRCm39)	T173A	probably benign	Het
Zfp69	A	T	4: 120,788,598 (GRCm39)	V239D	possibly damaging	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150,251,267 (GRCm39)	nonsense	probably null
IGL02566:Zfp442	APN	2	150,251,711 (GRCm39)	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150,251,714 (GRCm39)	splice site	probably benign
LCD18:Zfp442	UTSW	2	150,261,768 (GRCm39)	intron	probably benign
PIT4812001:Zfp442	UTSW	2	150,251,661 (GRCm39)	nonsense	probably null
R0219:Zfp442	UTSW	2	150,253,160 (GRCm39)	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150,253,169 (GRCm39)	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150,250,260 (GRCm39)	nonsense	probably null
R1702:Zfp442	UTSW	2	150,251,100 (GRCm39)	nonsense	probably null
R1829:Zfp442	UTSW	2	150,250,983 (GRCm39)	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150,250,100 (GRCm39)	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150,250,582 (GRCm39)	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150,250,042 (GRCm39)	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150,251,526 (GRCm39)	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150,250,149 (GRCm39)	missense	probably damaging	1.00
R4894:Zfp442	UTSW	2	150,253,130 (GRCm39)	critical splice donor site	probably null
R4932:Zfp442	UTSW	2	150,251,635 (GRCm39)	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150,250,415 (GRCm39)	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150,251,530 (GRCm39)	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150,250,079 (GRCm39)	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150,249,944 (GRCm39)	nonsense	probably null
R6042:Zfp442	UTSW	2	150,250,016 (GRCm39)	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150,293,321 (GRCm39)	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150,250,028 (GRCm39)	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150,251,499 (GRCm39)	missense	possibly damaging	0.72
R6931:Zfp442	UTSW	2	150,252,860 (GRCm39)	critical splice donor site	probably null
R7184:Zfp442	UTSW	2	150,250,056 (GRCm39)	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150,251,201 (GRCm39)	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150,250,925 (GRCm39)	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150,250,676 (GRCm39)	missense	unknown
R7591:Zfp442	UTSW	2	150,250,092 (GRCm39)	nonsense	probably null
R7679:Zfp442	UTSW	2	150,252,917 (GRCm39)	nonsense	probably null
R7768:Zfp442	UTSW	2	150,250,241 (GRCm39)	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150,251,639 (GRCm39)	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150,251,402 (GRCm39)	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150,253,146 (GRCm39)	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150,251,096 (GRCm39)	missense	possibly damaging	0.83
R8192:Zfp442	UTSW	2	150,250,629 (GRCm39)	missense	unknown
R8528:Zfp442	UTSW	2	150,250,962 (GRCm39)	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150,250,093 (GRCm39)	missense	probably benign	0.30
R9269:Zfp442	UTSW	2	150,251,287 (GRCm39)	missense	probably benign	0.19
R9371:Zfp442	UTSW	2	150,250,676 (GRCm39)	missense	unknown
R9401:Zfp442	UTSW	2	150,251,615 (GRCm39)	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150,250,668 (GRCm39)	missense	unknown
R9711:Zfp442	UTSW	2	150,250,207 (GRCm39)	missense	possibly damaging	0.93
Z1177:Zfp442	UTSW	2	150,250,399 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TAGTGTACATTCACAGTTTCCCTTCAG -3'
(R):5'- GCCTTTGCTCGAAACACTC -3'

Sequencing Primer
(F):5'- ACATTCACAGTTTCCCTTCAGTATGG -3'
(R):5'- AGAATACATACTGGGCAGAAACC -3'

Posted On

2019-05-13