Mutagenetix > Incidental Mutation

Incidental Mutation 'R7080:Gpr19'

549462

Institutional Source

Beutler Lab

Gene Symbol

Gpr19

Ensembl Gene

ENSMUSG00000032641

Gene Name

G protein-coupled receptor 19

Synonyms

MMRRC Submission

045174-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7080 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

134846055-134875157 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 134847419 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 88 (V88D)

Ref Sequence

ENSEMBL: ENSMUSP00000066287 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046255] [ENSMUST00000066107] [ENSMUST00000111932] [ENSMUST00000116515] [ENSMUST00000165392] [ENSMUST00000203409] [ENSMUST00000203762] [ENSMUST00000204880] [ENSMUST00000215088]

AlphaFold

Q61121

Predicted Effect

possibly damaging
Transcript: ENSMUST00000046255
AA Change: V2D

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000047630
Gene: ENSMUSG00000032641
AA Change: V2D

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	76	345	1.7e-12	PFAM
Pfam:7tm_1	82	330	1.8e-50	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000066107
AA Change: V88D

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000066287
Gene: ENSMUSG00000032641
AA Change: V88D

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	76	345	1.7e-12	PFAM
Pfam:7tm_1	82	330	5.3e-45	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111932

SMART Domains

Protein: ENSMUSP00000107563
Gene: ENSMUSG00000032641

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	70	339	2.6e-12	PFAM
Pfam:7tm_1	76	324	3.5e-50	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000116515

SMART Domains

Protein: ENSMUSP00000112214
Gene: ENSMUSG00000032641

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	70	339	2.6e-12	PFAM
Pfam:7tm_1	76	324	3.5e-50	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000165392

SMART Domains

Protein: ENSMUSP00000127876
Gene: ENSMUSG00000032641

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	70	339	2.6e-12	PFAM
Pfam:7tm_1	76	324	3.5e-50	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203409

SMART Domains

Protein: ENSMUSP00000145128
Gene: ENSMUSG00000032641

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	70	132	9e-5	PFAM
Pfam:7tm_1	76	135	2.6e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203762

Predicted Effect

probably benign
Transcript: ENSMUST00000204880

SMART Domains

Protein: ENSMUSP00000144918
Gene: ENSMUSG00000032641

Domain	Start	End	E-Value	Type
transmembrane domain	59	76	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000215088

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

94% (47/50)

MGI Phenotype

PHENOTYPE: Homozygous mutation of this gene results in increased anxiety-like behavior. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca2	A	G	2: 25,336,116 (GRCm39)	E2162G	probably benign	Het
Ackr4	A	G	9: 103,976,761 (GRCm39)	V62A	probably damaging	Het
Atox1	T	A	11: 55,341,365 (GRCm39)	K57*	probably null	Het
Atp6v1e1	G	A	6: 120,799,350 (GRCm39)		probably benign	Het
Bmpr2	T	C	1: 59,906,842 (GRCm39)	V645A	probably benign	Het
Brwd1	T	C	16: 95,810,730 (GRCm39)	T1602A	probably benign	Het
Cdc42bpg	T	C	19: 6,365,219 (GRCm39)	V692A	probably damaging	Het
Cdca2	T	C	14: 67,935,551 (GRCm39)	D388G	probably damaging	Het
Celsr1	G	A	15: 85,816,652 (GRCm39)	R1764C	possibly damaging	Het
Copz2	G	T	11: 96,747,538 (GRCm39)	V174L	probably benign	Het
Dnaaf1	T	C	8: 120,309,335 (GRCm39)	L141P	probably damaging	Het
Fam170a	T	A	18: 50,413,740 (GRCm39)		probably null	Het
Fshr	A	T	17: 89,404,539 (GRCm39)		probably null	Het
Gbx1	C	T	5: 24,731,298 (GRCm39)	A173T	probably benign	Het
Gm3604	G	A	13: 62,518,109 (GRCm39)	A83V	probably damaging	Het
Hnrnpd	C	T	5: 100,124,392 (GRCm39)		probably null	Het
Homez	T	C	14: 55,095,112 (GRCm39)	S199G	probably benign	Het
Kit	T	C	5: 75,767,941 (GRCm39)	I108T	probably damaging	Het
Lrrc34	A	C	3: 30,688,705 (GRCm39)	Y199D	probably damaging	Het
Mapk12	T	C	15: 89,017,350 (GRCm39)	D208G	probably damaging	Het
Mon1a	A	G	9: 107,778,985 (GRCm39)	D403G	probably damaging	Het
Myo1d	C	T	11: 80,565,460 (GRCm39)	E426K	probably damaging	Het
Nol11	G	A	11: 107,070,878 (GRCm39)	T307I	probably damaging	Het
Or10d5	A	G	9: 39,861,444 (GRCm39)	C208R	probably damaging	Het
Or52b4	A	T	7: 102,184,172 (GRCm39)	I73F	possibly damaging	Het
Or5aq1b	A	G	2: 86,902,083 (GRCm39)	Y132H	probably damaging	Het
Or6d15	A	T	6: 116,559,314 (GRCm39)	F198I	probably damaging	Het
Or8k28	A	T	2: 86,285,835 (GRCm39)	L260*	probably null	Het
Pcdhb16	C	T	18: 37,611,516 (GRCm39)	Q159*	probably null	Het
Phf19	G	A	2: 34,788,724 (GRCm39)		probably null	Het
Qrfpr	C	T	3: 36,234,198 (GRCm39)	R381H	probably benign	Het
Rad51ap2	A	G	12: 11,506,366 (GRCm39)	D96G	probably benign	Het
Ranbp1	T	C	16: 18,063,097 (GRCm39)	D93G	possibly damaging	Het
Reep1	A	G	6: 71,757,749 (GRCm39)	D116G	possibly damaging	Het
Rinl	T	C	7: 28,496,101 (GRCm39)	C361R	probably damaging	Het
Rps6kb1	T	C	11: 86,397,666 (GRCm39)	D393G	probably damaging	Het
Slc2a12	T	C	10: 22,541,216 (GRCm39)	V357A	probably benign	Het
Spryd3	T	C	15: 102,026,627 (GRCm39)	D348G	probably benign	Het
Syne2	G	A	12: 76,099,501 (GRCm39)	A569T	probably benign	Het
Tcstv5	T	C	13: 120,411,270 (GRCm39)	D112G	probably benign	Het
Thoc6	C	T	17: 23,892,503 (GRCm39)	R6Q	probably null	Het
Tyw3	G	C	3: 154,299,426 (GRCm39)	S94R	probably benign	Het
Unc13b	C	T	4: 43,171,926 (GRCm39)	T918I	unknown	Het
Unc80	A	T	1: 66,685,680 (GRCm39)	H2268L	possibly damaging	Het
Urod	C	T	4: 116,849,838 (GRCm39)	A187T	probably damaging	Het
Usp46	G	T	5: 74,177,344 (GRCm39)	N205K	probably benign	Het
Wapl	T	C	14: 34,414,313 (GRCm39)	F392L	probably benign	Het
Zim1	T	C	7: 6,680,305 (GRCm39)	T453A	possibly damaging	Het

Other mutations in Gpr19

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01310:Gpr19	APN	6	134,846,705 (GRCm39)	missense	probably damaging	0.99
IGL01373:Gpr19	APN	6	134,847,284 (GRCm39)	missense	possibly damaging	0.81
IGL01988:Gpr19	APN	6	134,846,247 (GRCm39)	missense	probably damaging	1.00
R1530:Gpr19	UTSW	6	134,846,961 (GRCm39)	missense	probably damaging	0.97
R1548:Gpr19	UTSW	6	134,847,047 (GRCm39)	missense	possibly damaging	0.92
R1699:Gpr19	UTSW	6	134,847,192 (GRCm39)	missense	possibly damaging	0.93
R2131:Gpr19	UTSW	6	134,847,405 (GRCm39)	start codon destroyed	probably null	0.99
R5016:Gpr19	UTSW	6	134,846,880 (GRCm39)	nonsense	probably null
R6605:Gpr19	UTSW	6	134,847,398 (GRCm39)	missense	probably benign	0.02
R7746:Gpr19	UTSW	6	134,846,355 (GRCm39)	missense	probably damaging	1.00
R8014:Gpr19	UTSW	6	134,846,436 (GRCm39)	missense	probably damaging	1.00
R9176:Gpr19	UTSW	6	134,846,718 (GRCm39)	missense	probably damaging	0.99
R9408:Gpr19	UTSW	6	134,864,704 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CTGAAGCTCTGTCCTGTTGC -3'
(R):5'- TTGCTGGGAGGAGACAGATTC -3'

Sequencing Primer
(F):5'- GAAGCTCTGTCCTGTTGCTCATC -3'
(R):5'- CTCGGTGAATGAGAATGGCTC -3'

Posted On

2019-05-15