Mutagenetix > Incidental Mutation

Incidental Mutation 'R7081:Psg21'

549528

Institutional Source

Beutler Lab

Gene Symbol

Psg21

Ensembl Gene

ENSMUSG00000070796

Gene Name

pregnancy-specific beta-1-glycoprotein 21

Synonyms

1600019C01Rik, 1600026N13Rik, 1600025N01Rik, cea8

MMRRC Submission

045175-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.049) question?

Stock #

R7081 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

18380661-18390650 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 18388774 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 106 (W106*)

Ref Sequence

ENSEMBL: ENSMUSP00000092387 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094793] [ENSMUST00000182128]

AlphaFold

Q9DAV5

Predicted Effect

probably null
Transcript: ENSMUST00000094793
AA Change: W106*

SMART Domains

Protein: ENSMUSP00000092387
Gene: ENSMUSG00000070796
AA Change: W106*

Domain	Start	End	E-Value	Type
IG	39	138	3.04e-3	SMART
IG	159	260	7.25e-1	SMART
IG	276	375	1.25e-4	SMART
IGc2	393	457	1.47e-10	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000182128
AA Change: W106*

SMART Domains

Protein: ENSMUSP00000138173
Gene: ENSMUSG00000070796
AA Change: W106*

Domain	Start	End	E-Value	Type
IG	39	138	3.04e-3	SMART
IG	159	260	7.25e-1	SMART
IG	276	375	1.25e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatf	T	A	11: 84,361,951 (GRCm39)	H337L	possibly damaging	Het
Abcb4	T	A	5: 8,984,263 (GRCm39)	N664K	probably benign	Het
Adh7	A	G	3: 137,934,606 (GRCm39)	D343G	probably benign	Het
Als2cl	G	A	9: 110,723,650 (GRCm39)	R682Q	possibly damaging	Het
Angptl6	A	C	9: 20,786,644 (GRCm39)	I334R	probably damaging	Het
Ankrd45	A	G	1: 160,978,863 (GRCm39)	N101D	probably benign	Het
Arhgef10	C	T	8: 15,047,547 (GRCm39)	Q1137*	probably null	Het
Asap3	T	A	4: 135,968,881 (GRCm39)		probably null	Het
Bmp2k	T	C	5: 97,212,820 (GRCm39)	S568P	unknown	Het
C4b	A	C	17: 34,954,417 (GRCm39)	F917L	probably benign	Het
Cacna1e	A	T	1: 154,576,129 (GRCm39)	V168E	possibly damaging	Het
Ccdc60	T	A	5: 116,264,146 (GRCm39)	I543F	probably benign	Het
Cd36	T	C	5: 18,019,702 (GRCm39)	D133G	probably damaging	Het
Chd4	T	C	6: 125,106,948 (GRCm39)	V1911A	unknown	Het
Cplx4	A	T	18: 66,100,538 (GRCm39)		probably null	Het
Cyp1a2	A	T	9: 57,586,272 (GRCm39)	D415E	possibly damaging	Het
Cyp3a57	T	C	5: 145,318,183 (GRCm39)	I388T	probably damaging	Het
Dnajc5b	T	C	3: 19,601,025 (GRCm39)		probably null	Het
Dock4	A	T	12: 40,671,285 (GRCm39)	I35F	probably damaging	Het
Efna4	A	T	3: 89,241,601 (GRCm39)	L206Q	unknown	Het
Eif2a	T	C	3: 58,449,139 (GRCm39)		probably null	Het
Fam120a	A	G	13: 49,063,801 (GRCm39)	F612L	probably damaging	Het
Fbxw21	A	G	9: 108,990,990 (GRCm39)	L23P	probably damaging	Het
Fcamr	A	T	1: 130,740,949 (GRCm39)	E456V	probably damaging	Het
Fkbp8	G	A	8: 70,983,644 (GRCm39)	R106H	probably benign	Het
Galr2	T	A	11: 116,173,874 (GRCm39)	L168Q	probably damaging	Het
Gm11111	C	T	5: 98,701,399 (GRCm39)	S22L	unknown	Het
Gnpat	T	C	8: 125,590,008 (GRCm39)	F11S	possibly damaging	Het
H2-T24	A	T	17: 36,328,344 (GRCm39)	D46E	probably damaging	Het
Idh2	GGTCCCAG	GG	7: 79,748,077 (GRCm39)		probably benign	Het
Ifna12	T	C	4: 88,521,440 (GRCm39)	R36G	probably damaging	Het
Kcnma1	G	A	14: 23,350,086 (GRCm39)	P1151L	probably damaging	Het
Kif5c	T	A	2: 49,631,373 (GRCm39)	D683E	probably benign	Het
Krit1	T	C	5: 3,873,651 (GRCm39)	Y477H	possibly damaging	Het
Lipm	T	A	19: 34,098,723 (GRCm39)	V399D	possibly damaging	Het
Lrguk	A	T	6: 34,079,074 (GRCm39)	T770S	probably benign	Het
Lrrc37a	T	C	11: 103,348,781 (GRCm39)	N2638S	unknown	Het
Lrrc40	T	C	3: 157,742,442 (GRCm39)	V27A	probably damaging	Het
Map4k1	T	C	7: 28,690,574 (GRCm39)	V355A	probably benign	Het
Mylk3	T	C	8: 86,091,422 (GRCm39)	I128V	probably benign	Het
Myo1c	A	G	11: 75,551,789 (GRCm39)	D289G	probably benign	Het
Mypn	A	G	10: 62,970,737 (GRCm39)	F889S	probably damaging	Het
Ndufv3	C	T	17: 31,746,407 (GRCm39)	P99L	possibly damaging	Het
Nlrp10	A	C	7: 108,523,855 (GRCm39)	S542A	probably benign	Het
Noc2l	T	A	4: 156,331,477 (GRCm39)	D718E	possibly damaging	Het
Ntng1	T	A	3: 109,759,105 (GRCm39)	I355F	probably benign	Het
Nup210	A	G	6: 91,037,647 (GRCm39)	V742A	possibly damaging	Het
Nup50l	A	G	6: 96,142,798 (GRCm39)	V82A	possibly damaging	Het
Olig2	T	A	16: 91,023,307 (GRCm39)	L7Q	probably damaging	Het
Or51f23b	A	T	7: 102,402,395 (GRCm39)	I247N	probably benign	Het
Or8u10	A	T	2: 85,915,939 (GRCm39)	F61I	probably damaging	Het
Parpbp	A	T	10: 87,929,517 (GRCm39)	W444R	probably damaging	Het
Plekhg3	A	G	12: 76,625,019 (GRCm39)	E1287G	probably benign	Het
Pramel16	T	C	4: 143,675,848 (GRCm39)	D326G	probably damaging	Het
Pramel56	T	A	5: 95,022,641 (GRCm39)	N107K	possibly damaging	Het
Prrc2b	A	T	2: 32,103,075 (GRCm39)	Q851L	probably benign	Het
Rab18	T	G	18: 6,778,529 (GRCm39)	D53E	probably benign	Het
Rbm19	T	A	5: 120,261,216 (GRCm39)		probably null	Het
Rhobtb1	T	C	10: 69,102,127 (GRCm39)	V136A	probably benign	Het
Rsph4a	G	A	10: 33,785,189 (GRCm39)	V367I	probably damaging	Het
Sec24b	T	C	3: 129,781,391 (GRCm39)	N1161S	probably benign	Het
Sgcd	C	A	11: 47,016,428 (GRCm39)	G145*	probably null	Het
Sin3a	A	G	9: 57,001,755 (GRCm39)	K134R	probably null	Het
Slc9b2	A	G	3: 135,027,698 (GRCm39)	E108G	probably benign	Het
Stk24	A	T	14: 121,531,706 (GRCm39)	S317T	probably benign	Het
Stra8	T	A	6: 34,911,302 (GRCm39)		probably null	Het
Tmem131	A	G	1: 36,928,376 (GRCm39)	V71A	possibly damaging	Het
Tmem87a	T	C	2: 120,211,264 (GRCm39)	D227G	possibly damaging	Het
Vnn1	T	A	10: 23,770,903 (GRCm39)	L44M	possibly damaging	Het
Wdr20	G	A	12: 110,769,884 (GRCm39)	V160I	possibly damaging	Het
Zfand4	T	A	6: 116,292,581 (GRCm39)	N667K	possibly damaging	Het
Zfp1005	A	G	2: 150,110,189 (GRCm39)	H293R	possibly damaging	Het
Zfp609	A	G	9: 65,609,723 (GRCm39)	V1080A	possibly damaging	Het
Zhx1	C	T	15: 57,917,734 (GRCm39)	V171I	probably benign	Het

Other mutations in Psg21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01571:Psg21	APN	7	18,388,750 (GRCm39)	missense	probably damaging	1.00
IGL02390:Psg21	APN	7	18,386,556 (GRCm39)	missense	probably benign	0.11
IGL02548:Psg21	APN	7	18,388,961 (GRCm39)	missense	possibly damaging	0.89
IGL03001:Psg21	APN	7	18,386,410 (GRCm39)	missense	probably benign	0.03
IGL03135:Psg21	APN	7	18,388,843 (GRCm39)	missense	probably benign	0.00
R0131:Psg21	UTSW	7	18,388,793 (GRCm39)	missense	probably benign	0.39
R0551:Psg21	UTSW	7	18,386,565 (GRCm39)	critical splice acceptor site	probably null
R1512:Psg21	UTSW	7	18,390,425 (GRCm39)	missense	probably benign	0.00
R1874:Psg21	UTSW	7	18,384,741 (GRCm39)	missense	probably benign	0.15
R1993:Psg21	UTSW	7	18,388,695 (GRCm39)	missense	probably benign	0.04
R2327:Psg21	UTSW	7	18,386,378 (GRCm39)	missense	possibly damaging	0.61
R3414:Psg21	UTSW	7	18,386,305 (GRCm39)	missense	probably damaging	1.00
R4422:Psg21	UTSW	7	18,381,257 (GRCm39)	missense	probably damaging	1.00
R5138:Psg21	UTSW	7	18,390,453 (GRCm39)	start codon destroyed	probably null	0.94
R5623:Psg21	UTSW	7	18,388,939 (GRCm39)	missense	probably damaging	1.00
R5686:Psg21	UTSW	7	18,386,183 (GRCm39)	intron	probably benign
R6166:Psg21	UTSW	7	18,390,664 (GRCm39)	unclassified	probably benign
R6177:Psg21	UTSW	7	18,386,279 (GRCm39)	missense	possibly damaging	0.64
R6190:Psg21	UTSW	7	18,388,926 (GRCm39)	missense	possibly damaging	0.61
R6210:Psg21	UTSW	7	18,386,270 (GRCm39)	missense	probably damaging	1.00
R6482:Psg21	UTSW	7	18,388,664 (GRCm39)	splice site	probably null
R6729:Psg21	UTSW	7	18,386,516 (GRCm39)	missense	probably damaging	1.00
R6866:Psg21	UTSW	7	18,386,209 (GRCm39)	missense	probably damaging	1.00
R6992:Psg21	UTSW	7	18,388,668 (GRCm39)	critical splice donor site	probably null
R7075:Psg21	UTSW	7	18,388,786 (GRCm39)	missense	probably damaging	1.00
R7098:Psg21	UTSW	7	18,386,470 (GRCm39)	missense	probably damaging	1.00
R7582:Psg21	UTSW	7	18,381,128 (GRCm39)	makesense	probably null
R7588:Psg21	UTSW	7	18,381,134 (GRCm39)	missense	probably benign	0.00
R7607:Psg21	UTSW	7	18,388,708 (GRCm39)	missense	probably benign	0.02
R7830:Psg21	UTSW	7	18,381,223 (GRCm39)	missense	probably damaging	1.00
R7964:Psg21	UTSW	7	18,381,136 (GRCm39)	missense	probably benign	0.01
R8758:Psg21	UTSW	7	18,384,678 (GRCm39)	missense	probably damaging	1.00
R8972:Psg21	UTSW	7	18,381,293 (GRCm39)	missense	probably benign	0.03
R8988:Psg21	UTSW	7	18,386,389 (GRCm39)	missense	probably benign	0.00
R9119:Psg21	UTSW	7	18,381,409 (GRCm39)	missense	probably benign	0.14
R9446:Psg21	UTSW	7	18,388,865 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGGCCTGATACTTCTATGTGCC -3'
(R):5'- CACCACTGCTAGTGTCACCATC -3'

Sequencing Primer
(F):5'- CCTGATACTTCTATGTGCCGAGTG -3'
(R):5'- GCTAGTGTCACCATCCAGTCAC -3'

Posted On

2019-05-15