Mutagenetix > Incidental Mutation

Incidental Mutation 'R7083:Shroom3'

549667

Institutional Source

Beutler Lab

Gene Symbol

Shroom3

Ensembl Gene

ENSMUSG00000029381

Gene Name

shroom family member 3

Synonyms

Shrm3, D5Ertd287e, Shrm

MMRRC Submission

045177-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7083 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

92831294-93113177 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 93112384 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 1915 (L1915P)

Ref Sequence

ENSEMBL: ENSMUSP00000108678 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000113051] [ENSMUST00000113054] [ENSMUST00000113055] [ENSMUST00000168878] [ENSMUST00000225438]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000113051
AA Change: L1740P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108674
Gene: ENSMUSG00000029381
AA Change: L1740P

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
low complexity region	83	93	N/A	INTRINSIC
low complexity region	572	586	N/A	INTRINSIC
low complexity region	621	639	N/A	INTRINSIC
low complexity region	685	704	N/A	INTRINSIC
Pfam:ASD1	706	885	2.3e-65	PFAM
low complexity region	939	952	N/A	INTRINSIC
low complexity region	1132	1143	N/A	INTRINSIC
low complexity region	1172	1184	N/A	INTRINSIC
low complexity region	1274	1288	N/A	INTRINSIC
low complexity region	1333	1345	N/A	INTRINSIC
Pfam:ASD2	1478	1765	3.1e-107	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000113054
AA Change: L1740P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108677
Gene: ENSMUSG00000029381
AA Change: L1740P

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
low complexity region	83	93	N/A	INTRINSIC
low complexity region	572	586	N/A	INTRINSIC
low complexity region	621	639	N/A	INTRINSIC
low complexity region	685	704	N/A	INTRINSIC
Pfam:ASD1	706	885	2.3e-65	PFAM
low complexity region	939	952	N/A	INTRINSIC
low complexity region	1132	1143	N/A	INTRINSIC
low complexity region	1172	1184	N/A	INTRINSIC
low complexity region	1274	1288	N/A	INTRINSIC
low complexity region	1333	1345	N/A	INTRINSIC
Pfam:ASD2	1478	1765	3.1e-107	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000113055
AA Change: L1915P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108678
Gene: ENSMUSG00000029381
AA Change: L1915P

Domain	Start	End	E-Value	Type
PDZ	35	109	5.81e-11	SMART
low complexity region	191	202	N/A	INTRINSIC
low complexity region	258	268	N/A	INTRINSIC
low complexity region	747	761	N/A	INTRINSIC
low complexity region	796	814	N/A	INTRINSIC
low complexity region	860	879	N/A	INTRINSIC
Pfam:ASD1	882	1060	1e-57	PFAM
low complexity region	1114	1127	N/A	INTRINSIC
low complexity region	1307	1318	N/A	INTRINSIC
low complexity region	1347	1359	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1508	1520	N/A	INTRINSIC
Pfam:ASD2	1654	1940	9.9e-112	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000168878
AA Change: L1784P

SMART Domains

Protein: ENSMUSP00000130419
Gene: ENSMUSG00000029381
AA Change: L1784P

Domain	Start	End	E-Value	Type
PDZ	35	109	5.81e-11	SMART
low complexity region	191	202	N/A	INTRINSIC
low complexity region	258	268	N/A	INTRINSIC
low complexity region	747	761	N/A	INTRINSIC
low complexity region	796	814	N/A	INTRINSIC
low complexity region	860	879	N/A	INTRINSIC
low complexity region	983	996	N/A	INTRINSIC
low complexity region	1176	1187	N/A	INTRINSIC
low complexity region	1216	1228	N/A	INTRINSIC
low complexity region	1318	1332	N/A	INTRINSIC
low complexity region	1377	1389	N/A	INTRINSIC
Pfam:ASD2	1522	1809	8.9e-108	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000225438
AA Change: L1834P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a PDZ-domain-containing protein that belongs to a family of Shroom-related proteins. This protein may be involved in regulating cell shape in certain tissues. A similar protein in mice is required for proper neurulation. [provided by RefSeq, Jan 2011]
PHENOTYPE: Homozygous mutation of this locus results in failed neural tube closure leading to exencephaly, acrania, facial clefting, and spina bifida. Homozygotes develop to term but die either at birth or shortly thereafter. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam21	A	T	12: 81,607,015 (GRCm39)	M249K	possibly damaging	Het
Arhgef38	T	A	3: 132,838,197 (GRCm39)	Q613L	unknown	Het
Arpp21	A	G	9: 112,012,612 (GRCm39)	V70A	probably benign	Het
AW551984	T	C	9: 39,508,943 (GRCm39)	N327S	probably damaging	Het
Bnc1	T	C	7: 81,623,058 (GRCm39)	K723R	probably damaging	Het
Btbd7	A	G	12: 102,754,594 (GRCm39)	L724P	probably damaging	Het
Btnl10	G	T	11: 58,809,963 (GRCm39)	V35F	probably damaging	Het
Cd22	T	A	7: 30,567,473 (GRCm39)	T704S	probably damaging	Het
Cd4	T	G	6: 124,847,535 (GRCm39)	S210R	probably benign	Het
Cped1	A	G	6: 22,123,579 (GRCm39)	Q444R	probably benign	Het
Dusp26	A	G	8: 31,581,747 (GRCm39)		probably benign	Het
Dync1i1	C	T	6: 5,969,429 (GRCm39)	A418V	probably damaging	Het
Fibp	A	G	19: 5,513,659 (GRCm39)	D232G	probably damaging	Het
Frem2	T	A	3: 53,444,914 (GRCm39)	T2406S	probably damaging	Het
Gm40460	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 141,794,450 (GRCm39)		probably benign	Het
Gpr39	T	A	1: 125,605,155 (GRCm39)	W28R	probably damaging	Het
Greb1	A	G	12: 16,773,315 (GRCm39)	V253A	probably benign	Het
Hexim1	T	G	11: 103,007,992 (GRCm39)	L82W	possibly damaging	Het
Hmga1	G	T	17: 27,779,945 (GRCm39)	R49L	possibly damaging	Het
Irag2	A	T	6: 145,115,509 (GRCm39)	N349I	probably damaging	Het
Itch	A	T	2: 155,052,364 (GRCm39)	N655Y	probably damaging	Het
Izumo2	A	G	7: 44,359,757 (GRCm39)	E129G	probably damaging	Het
Klk1b24	G	A	7: 43,841,225 (GRCm39)	C186Y	probably damaging	Het
Lnx1	G	A	5: 74,788,846 (GRCm39)	S31F	possibly damaging	Het
Lrrc37a	T	C	11: 103,394,166 (GRCm39)	I420V	probably benign	Het
Ltk	A	C	2: 119,582,555 (GRCm39)	C776G	probably damaging	Het
Mast4	A	C	13: 102,874,223 (GRCm39)	L1715R	probably damaging	Het
Med28	T	C	5: 45,680,878 (GRCm39)		probably null	Het
Naf1	GCCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGA	GCCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGA	8: 67,313,138 (GRCm39)		probably benign	Het
Nckap1l	C	A	15: 103,390,551 (GRCm39)	T774K	probably damaging	Het
Nfkbiz	T	C	16: 55,638,663 (GRCm39)	K266E	possibly damaging	Het
Ntrk3	T	A	7: 77,900,587 (GRCm39)	D584V	probably damaging	Het
Or1o1	T	C	17: 37,717,063 (GRCm39)	V208A	probably benign	Het
Or2h15	T	A	17: 38,441,601 (GRCm39)	T161S	probably benign	Het
Or4c126	A	G	2: 89,824,201 (GRCm39)	I155V	probably benign	Het
Or4n4b	G	A	14: 50,536,736 (GRCm39)	T10I	possibly damaging	Het
Or5h25	T	C	16: 58,930,400 (GRCm39)	D191G	probably damaging	Het
Picalm	T	A	7: 89,825,976 (GRCm39)	I376K	probably benign	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Prps1l3	A	T	12: 57,286,034 (GRCm39)	I275L	probably benign	Het
Psg26	G	A	7: 18,213,934 (GRCm39)	R243*	probably null	Het
Ranbp2	A	G	10: 58,315,052 (GRCm39)	H1924R	probably damaging	Het
Rasef	T	C	4: 73,709,221 (GRCm39)	D4G	probably benign	Het
Rttn	T	C	18: 89,108,722 (GRCm39)	L1642P	probably damaging	Het
Slc26a10	A	G	10: 127,013,037 (GRCm39)	V319A	probably damaging	Het
Slc4a10	A	G	2: 62,064,839 (GRCm39)	N231S	probably benign	Het
Sox18	T	C	2: 181,312,165 (GRCm39)	Q322R	possibly damaging	Het
Sting1	T	C	18: 35,867,703 (GRCm39)	H331R	probably damaging	Het
Syde1	G	T	10: 78,422,903 (GRCm39)	P490T	probably benign	Het
Syne2	A	G	12: 75,990,662 (GRCm39)	I1881M	probably damaging	Het
Taf1c	T	C	8: 120,327,407 (GRCm39)	D387G	probably damaging	Het
Tenm4	T	A	7: 96,544,556 (GRCm39)	Y2228N	probably damaging	Het
Tubgcp5	C	T	7: 55,450,443 (GRCm39)	Q185*	probably null	Het
Vmn2r44	T	A	7: 8,381,369 (GRCm39)	I175F	probably benign	Het
Zdhhc7	C	A	8: 120,812,166 (GRCm39)	C152F	probably damaging	Het
Zfp52	T	A	17: 21,780,392 (GRCm39)	M80K	possibly damaging	Het
Zfp612	T	C	8: 110,815,768 (GRCm39)	I325T	probably damaging	Het
Zmiz1	T	G	14: 25,652,372 (GRCm39)	F597V	probably damaging	Het

Other mutations in Shroom3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00850:Shroom3	APN	5	93,098,924 (GRCm39)	missense	probably damaging	1.00
IGL01086:Shroom3	APN	5	93,096,311 (GRCm39)	missense	probably benign	0.01
IGL01363:Shroom3	APN	5	93,088,852 (GRCm39)	missense	probably benign	0.01
IGL01468:Shroom3	APN	5	93,088,201 (GRCm39)	missense	probably damaging	1.00
IGL01675:Shroom3	APN	5	93,089,539 (GRCm39)	missense	probably damaging	0.99
IGL01862:Shroom3	APN	5	93,110,148 (GRCm39)	missense	probably damaging	1.00
IGL01987:Shroom3	APN	5	93,090,048 (GRCm39)	missense	probably damaging	0.99
IGL02104:Shroom3	APN	5	93,088,248 (GRCm39)	missense	probably benign	0.32
IGL03248:Shroom3	APN	5	93,100,399 (GRCm39)	missense	probably benign	0.00
IGL03386:Shroom3	APN	5	93,096,342 (GRCm39)	splice site	probably benign
R0167:Shroom3	UTSW	5	93,096,254 (GRCm39)	splice site	probably benign
R0388:Shroom3	UTSW	5	93,099,152 (GRCm39)	missense	probably benign	0.39
R0395:Shroom3	UTSW	5	92,928,762 (GRCm39)	missense	probably damaging	1.00
R0567:Shroom3	UTSW	5	93,112,312 (GRCm39)	missense	possibly damaging	0.53
R1496:Shroom3	UTSW	5	93,090,693 (GRCm39)	missense	possibly damaging	0.69
R1772:Shroom3	UTSW	5	93,088,515 (GRCm39)	missense	probably damaging	0.97
R1845:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R1921:Shroom3	UTSW	5	93,110,224 (GRCm39)	critical splice donor site	probably null
R2059:Shroom3	UTSW	5	92,831,643 (GRCm39)	missense	probably damaging	1.00
R2203:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2204:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2205:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2301:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2344:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2345:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2346:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2348:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2371:Shroom3	UTSW	5	92,928,729 (GRCm39)	missense	probably damaging	1.00
R2435:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2829:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2830:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2831:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2897:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R2898:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3079:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3080:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3433:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3729:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3730:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3735:Shroom3	UTSW	5	93,112,303 (GRCm39)	missense	possibly damaging	0.84
R3736:Shroom3	UTSW	5	93,112,303 (GRCm39)	missense	possibly damaging	0.84
R3851:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3852:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3943:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R3969:Shroom3	UTSW	5	93,088,738 (GRCm39)	missense	probably benign	0.05
R4008:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4009:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4012:Shroom3	UTSW	5	93,096,342 (GRCm39)	splice site	probably benign
R4154:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4157:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4172:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4173:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4201:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4202:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4204:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4205:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4206:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4284:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4285:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4364:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4384:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4456:Shroom3	UTSW	5	93,088,858 (GRCm39)	missense	probably benign	0.14
R4707:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4712:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4751:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4755:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4760:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4773:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4774:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4776:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4801:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4802:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4856:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4857:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4860:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4860:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4882:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R4883:Shroom3	UTSW	5	93,098,993 (GRCm39)	missense	probably benign	0.14
R4886:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R5262:Shroom3	UTSW	5	93,112,432 (GRCm39)	missense	probably damaging	1.00
R5271:Shroom3	UTSW	5	93,110,107 (GRCm39)	missense	probably damaging	1.00
R5719:Shroom3	UTSW	5	93,090,877 (GRCm39)	missense	probably benign	0.04
R5726:Shroom3	UTSW	5	93,090,864 (GRCm39)	missense	probably benign	0.00
R5993:Shroom3	UTSW	5	93,088,047 (GRCm39)	missense	probably damaging	1.00
R6078:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R6079:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R6138:Shroom3	UTSW	5	93,090,945 (GRCm39)	missense	probably damaging	1.00
R6153:Shroom3	UTSW	5	93,112,267 (GRCm39)	missense	probably damaging	0.99
R6493:Shroom3	UTSW	5	93,089,420 (GRCm39)	missense	probably benign	0.03
R6495:Shroom3	UTSW	5	93,089,928 (GRCm39)	missense	possibly damaging	0.66
R6693:Shroom3	UTSW	5	93,088,617 (GRCm39)	missense	possibly damaging	0.61
R6801:Shroom3	UTSW	5	93,088,795 (GRCm39)	missense	probably damaging	1.00
R6893:Shroom3	UTSW	5	93,090,063 (GRCm39)	missense	probably damaging	0.97
R6912:Shroom3	UTSW	5	93,090,876 (GRCm39)	missense	probably benign	0.02
R6924:Shroom3	UTSW	5	93,112,262 (GRCm39)	missense	probably damaging	1.00
R7197:Shroom3	UTSW	5	93,090,463 (GRCm39)	missense	probably damaging	1.00
R7366:Shroom3	UTSW	5	93,112,465 (GRCm39)	nonsense	probably null
R7712:Shroom3	UTSW	5	93,098,806 (GRCm39)	missense	probably benign	0.01
R7725:Shroom3	UTSW	5	93,089,512 (GRCm39)	missense	probably benign	0.19
R7728:Shroom3	UTSW	5	92,831,566 (GRCm39)	missense	possibly damaging	0.73
R7774:Shroom3	UTSW	5	93,098,348 (GRCm39)	missense	probably damaging	0.98
R7795:Shroom3	UTSW	5	93,067,508 (GRCm39)	missense	probably damaging	0.99
R7821:Shroom3	UTSW	5	93,088,705 (GRCm39)	missense	probably damaging	0.98
R7971:Shroom3	UTSW	5	93,098,933 (GRCm39)	missense	probably damaging	1.00
R8276:Shroom3	UTSW	5	93,088,339 (GRCm39)	missense	probably damaging	0.99
R8934:Shroom3	UTSW	5	93,089,584 (GRCm39)	missense	probably damaging	1.00
R8938:Shroom3	UTSW	5	93,090,930 (GRCm39)	missense	probably damaging	1.00
R9083:Shroom3	UTSW	5	93,098,533 (GRCm39)	missense	probably damaging	0.97
R9108:Shroom3	UTSW	5	93,087,975 (GRCm39)	missense	probably damaging	1.00
R9124:Shroom3	UTSW	5	93,112,401 (GRCm39)	missense	probably benign	0.19
R9295:Shroom3	UTSW	5	93,098,478 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TAGAGCACTGGTTTGCAGCC -3'
(R):5'- GTCAACGTTGGGAAAACACC -3'

Sequencing Primer
(F):5'- TTACCCTGGCAGAGTTCTCTGAAC -3'
(R):5'- GTTGGGAAAACACCACCGAATACAG -3'

Posted On

2019-05-15