Incidental Mutation 'R7083:Sting1'
ID 549712
Institutional Source Beutler Lab
Gene Symbol Sting1
Ensembl Gene ENSMUSG00000024349
Gene Name stimulator of interferon response cGAMP interactor 1
Synonyms Tmem173, MPYS, Sting, 2610307O08Rik, ERIS
MMRRC Submission 045177-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R7083 (G1)
Quality Score 225.009
Status Not validated
Chromosome 18
Chromosomal Location 35866732-35873607 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 35867703 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Histidine to Arginine at position 331 (H331R)
Ref Sequence ENSEMBL: ENSMUSP00000111393 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000097617] [ENSMUST00000115728]
AlphaFold Q3TBT3
PDB Structure Immune activator bound to receptor [X-RAY DIFFRACTION]
mSTING/c-di-GMP [X-RAY DIFFRACTION]
mSTING [X-RAY DIFFRACTION]
Crystal structure of mSting in complex with c[G(2',5')pA(3',5')p] [X-RAY DIFFRACTION]
Crystal structure of mSting in complex with c[G(3',5')pA(3',5')p] [X-RAY DIFFRACTION]
Crystal structure of mSting in complex with DMXAA [X-RAY DIFFRACTION]
Predicted Effect probably benign
Transcript: ENSMUST00000097617
SMART Domains Protein: ENSMUSP00000095222
Gene: ENSMUSG00000073598

DomainStartEndE-ValueType
transmembrane domain 39 61 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000115728
AA Change: H331R

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000111393
Gene: ENSMUSG00000024349
AA Change: H331R

DomainStartEndE-ValueType
transmembrane domain 20 37 N/A INTRINSIC
Pfam:TMEM173 44 336 4.7e-125 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to viral infection and abnormal innate immunity. Mice homozygous for an ENU-induced allele exhibit altered response to bacterial and viral infection. [provided by MGI curators]
Allele List at MGI

All alleles(4) : Targeted(3) Chemically induced(1)
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam21 A T 12: 81,607,015 (GRCm39) M249K possibly damaging Het
Arhgef38 T A 3: 132,838,197 (GRCm39) Q613L unknown Het
Arpp21 A G 9: 112,012,612 (GRCm39) V70A probably benign Het
AW551984 T C 9: 39,508,943 (GRCm39) N327S probably damaging Het
Bnc1 T C 7: 81,623,058 (GRCm39) K723R probably damaging Het
Btbd7 A G 12: 102,754,594 (GRCm39) L724P probably damaging Het
Btnl10 G T 11: 58,809,963 (GRCm39) V35F probably damaging Het
Cd22 T A 7: 30,567,473 (GRCm39) T704S probably damaging Het
Cd4 T G 6: 124,847,535 (GRCm39) S210R probably benign Het
Cped1 A G 6: 22,123,579 (GRCm39) Q444R probably benign Het
Dusp26 A G 8: 31,581,747 (GRCm39) probably benign Het
Dync1i1 C T 6: 5,969,429 (GRCm39) A418V probably damaging Het
Fibp A G 19: 5,513,659 (GRCm39) D232G probably damaging Het
Frem2 T A 3: 53,444,914 (GRCm39) T2406S probably damaging Het
Gm40460 ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG 7: 141,794,450 (GRCm39) probably benign Het
Gpr39 T A 1: 125,605,155 (GRCm39) W28R probably damaging Het
Greb1 A G 12: 16,773,315 (GRCm39) V253A probably benign Het
Hexim1 T G 11: 103,007,992 (GRCm39) L82W possibly damaging Het
Hmga1 G T 17: 27,779,945 (GRCm39) R49L possibly damaging Het
Irag2 A T 6: 145,115,509 (GRCm39) N349I probably damaging Het
Itch A T 2: 155,052,364 (GRCm39) N655Y probably damaging Het
Izumo2 A G 7: 44,359,757 (GRCm39) E129G probably damaging Het
Klk1b24 G A 7: 43,841,225 (GRCm39) C186Y probably damaging Het
Lnx1 G A 5: 74,788,846 (GRCm39) S31F possibly damaging Het
Lrrc37a T C 11: 103,394,166 (GRCm39) I420V probably benign Het
Ltk A C 2: 119,582,555 (GRCm39) C776G probably damaging Het
Mast4 A C 13: 102,874,223 (GRCm39) L1715R probably damaging Het
Med28 T C 5: 45,680,878 (GRCm39) probably null Het
Naf1 GCCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGA GCCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGA 8: 67,313,138 (GRCm39) probably benign Het
Nckap1l C A 15: 103,390,551 (GRCm39) T774K probably damaging Het
Nfkbiz T C 16: 55,638,663 (GRCm39) K266E possibly damaging Het
Ntrk3 T A 7: 77,900,587 (GRCm39) D584V probably damaging Het
Or1o1 T C 17: 37,717,063 (GRCm39) V208A probably benign Het
Or2h15 T A 17: 38,441,601 (GRCm39) T161S probably benign Het
Or4c126 A G 2: 89,824,201 (GRCm39) I155V probably benign Het
Or4n4b G A 14: 50,536,736 (GRCm39) T10I possibly damaging Het
Or5h25 T C 16: 58,930,400 (GRCm39) D191G probably damaging Het
Picalm T A 7: 89,825,976 (GRCm39) I376K probably benign Het
Pkd1l3 GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA 8: 110,350,827 (GRCm39) probably benign Het
Prps1l3 A T 12: 57,286,034 (GRCm39) I275L probably benign Het
Psg26 G A 7: 18,213,934 (GRCm39) R243* probably null Het
Ranbp2 A G 10: 58,315,052 (GRCm39) H1924R probably damaging Het
Rasef T C 4: 73,709,221 (GRCm39) D4G probably benign Het
Rttn T C 18: 89,108,722 (GRCm39) L1642P probably damaging Het
Shroom3 T C 5: 93,112,384 (GRCm39) L1915P probably damaging Het
Slc26a10 A G 10: 127,013,037 (GRCm39) V319A probably damaging Het
Slc4a10 A G 2: 62,064,839 (GRCm39) N231S probably benign Het
Sox18 T C 2: 181,312,165 (GRCm39) Q322R possibly damaging Het
Syde1 G T 10: 78,422,903 (GRCm39) P490T probably benign Het
Syne2 A G 12: 75,990,662 (GRCm39) I1881M probably damaging Het
Taf1c T C 8: 120,327,407 (GRCm39) D387G probably damaging Het
Tenm4 T A 7: 96,544,556 (GRCm39) Y2228N probably damaging Het
Tubgcp5 C T 7: 55,450,443 (GRCm39) Q185* probably null Het
Vmn2r44 T A 7: 8,381,369 (GRCm39) I175F probably benign Het
Zdhhc7 C A 8: 120,812,166 (GRCm39) C152F probably damaging Het
Zfp52 T A 17: 21,780,392 (GRCm39) M80K possibly damaging Het
Zfp612 T C 8: 110,815,768 (GRCm39) I325T probably damaging Het
Zmiz1 T G 14: 25,652,372 (GRCm39) F597V probably damaging Het
Other mutations in Sting1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00977:Sting1 APN 18 35,867,620 (GRCm39) missense probably damaging 0.99
R0226:Sting1 UTSW 18 35,872,141 (GRCm39) missense probably benign
R0388:Sting1 UTSW 18 35,868,164 (GRCm39) splice site probably null
R0924:Sting1 UTSW 18 35,868,154 (GRCm39) critical splice donor site probably null
R2102:Sting1 UTSW 18 35,868,290 (GRCm39) missense probably damaging 1.00
R4159:Sting1 UTSW 18 35,872,272 (GRCm39) missense probably damaging 1.00
R4604:Sting1 UTSW 18 35,871,743 (GRCm39) missense probably damaging 0.97
R6209:Sting1 UTSW 18 35,869,155 (GRCm39) missense probably damaging 1.00
R6866:Sting1 UTSW 18 35,872,482 (GRCm39) missense probably damaging 0.97
R7008:Sting1 UTSW 18 35,868,224 (GRCm39) missense probably damaging 1.00
R7492:Sting1 UTSW 18 35,871,766 (GRCm39) missense probably damaging 1.00
R7726:Sting1 UTSW 18 35,868,318 (GRCm39) missense probably damaging 1.00
R7899:Sting1 UTSW 18 35,867,626 (GRCm39) missense probably damaging 1.00
R8424:Sting1 UTSW 18 35,872,223 (GRCm39) missense probably benign 0.10
R9084:Sting1 UTSW 18 35,869,155 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GATCACTAGAGCCCAGACAAGG -3'
(R):5'- ATCATCTCTAAAGCAGGAGGGTG -3'

Sequencing Primer
(F):5'- ACAAGGCTGTCCCATGC -3'
(R):5'- GGGCTTCCATGACTTTGATAAC -3'
Posted On 2019-05-15