Mutagenetix > Incidental Mutation

Incidental Mutation 'R7121:Plcd4'

551979

Institutional Source

Beutler Lab

Gene Symbol

Plcd4

Ensembl Gene

ENSMUSG00000026173

Gene Name

phospholipase C, delta 4

Synonyms

4921507K24Rik

MMRRC Submission

045210-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7121 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

74581175-74605137 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 74604524 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 767 (E767K)

Ref Sequence

ENSEMBL: ENSMUSP00000109379 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027315] [ENSMUST00000027362] [ENSMUST00000066986] [ENSMUST00000067916] [ENSMUST00000113737] [ENSMUST00000113747] [ENSMUST00000113749] [ENSMUST00000113750] [ENSMUST00000127921] [ENSMUST00000141412] [ENSMUST00000152707] [ENSMUST00000156613]

AlphaFold

Q8K3R3

Predicted Effect

probably benign
Transcript: ENSMUST00000027315

SMART Domains

Protein: ENSMUSP00000027315
Gene: ENSMUSG00000026135

Domain	Start	End	E-Value	Type
ZnF_C2H2	103	127	1.16e1	SMART
ZnF_C2H2	164	186	1.26e-2	SMART
ZnF_C2H2	193	218	7.78e-3	SMART
ZnF_C2H2	223	247	2.29e0	SMART
ZnF_C2H2	250	272	9.96e-1	SMART
low complexity region	317	329	N/A	INTRINSIC
ZnF_C2H2	363	385	9.96e-1	SMART
ZnF_C2H2	391	411	1.26e1	SMART
ZnF_C2H2	419	442	1.47e-3	SMART
ZnF_C2H2	453	475	2.75e-3	SMART
ZnF_C2H2	486	511	3.34e-2	SMART
ZnF_C2H2	516	540	4.81e0	SMART
ZnF_C2H2	543	566	7.05e-1	SMART
ZnF_C2H2	572	595	2.17e-1	SMART
ZnF_C2H2	601	623	1.56e-2	SMART
ZnF_C2H2	629	651	8.94e-3	SMART
ZnF_C2H2	657	679	1.38e-3	SMART
ZnF_C2H2	685	707	9.44e-2	SMART
ZnF_C2H2	712	735	1.26e-2	SMART
ZnF_C2H2	744	767	1.31e0	SMART
ZnF_C2H2	773	796	2.63e0	SMART
low complexity region	903	919	N/A	INTRINSIC
low complexity region	953	969	N/A	INTRINSIC
low complexity region	996	1007	N/A	INTRINSIC
ZnF_C2H2	1039	1059	2.01e1	SMART
ZnF_C2H2	1069	1089	1.91e1	SMART
low complexity region	1147	1160	N/A	INTRINSIC
ZnF_C2H2	1187	1207	2.7e2	SMART
low complexity region	1209	1222	N/A	INTRINSIC
ZnF_C2H2	1265	1285	3.56e1	SMART
ZnF_C2H2	1295	1318	4.98e-1	SMART
ZnF_C2H2	1331	1354	2.49e-1	SMART
ZnF_C2H2	1360	1382	1.03e-2	SMART
ZnF_C2H2	1388	1411	5.72e-1	SMART
ZnF_C2H2	1417	1440	6.75e0	SMART
ZnF_C2H2	1446	1469	9.58e-3	SMART
ZnF_C2H2	1488	1511	1.64e-1	SMART
ZnF_C2H2	1514	1536	1.1e-2	SMART
ZnF_C2H2	1540	1563	4.05e-1	SMART
ZnF_C2H2	1580	1602	4.45e0	SMART
ZnF_C2H2	1608	1630	8.81e-2	SMART
ZnF_C2H2	1636	1658	1.18e-2	SMART
ZnF_C2H2	1664	1686	1.2e-3	SMART
ZnF_C2H2	1692	1715	3.89e-3	SMART
ZnF_C2H2	1721	1743	4.54e-4	SMART
ZnF_C2H2	1749	1771	1.18e-2	SMART
ZnF_C2H2	1777	1799	3.52e-1	SMART
low complexity region	1829	1838	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000027362

SMART Domains

Protein: ENSMUSP00000027362
Gene: ENSMUSG00000026173

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC
PLCYc	538	654	1.26e-75	SMART
C2	673	779	5.68e-20	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000066986

SMART Domains

Protein: ENSMUSP00000065149
Gene: ENSMUSG00000026135

Domain	Start	End	E-Value	Type
low complexity region	116	128	N/A	INTRINSIC
ZnF_C2H2	162	184	9.96e-1	SMART
ZnF_C2H2	190	210	1.26e1	SMART
ZnF_C2H2	218	241	1.47e-3	SMART
ZnF_C2H2	252	274	2.75e-3	SMART
ZnF_C2H2	285	310	3.34e-2	SMART
ZnF_C2H2	315	339	4.81e0	SMART
ZnF_C2H2	342	365	7.05e-1	SMART
ZnF_C2H2	371	394	2.17e-1	SMART
ZnF_C2H2	400	422	1.56e-2	SMART
ZnF_C2H2	428	450	8.94e-3	SMART
ZnF_C2H2	456	478	1.38e-3	SMART
ZnF_C2H2	484	506	9.44e-2	SMART
ZnF_C2H2	511	534	1.26e-2	SMART
ZnF_C2H2	543	566	1.31e0	SMART
ZnF_C2H2	572	595	2.63e0	SMART
low complexity region	702	718	N/A	INTRINSIC
low complexity region	752	768	N/A	INTRINSIC
low complexity region	795	806	N/A	INTRINSIC
ZnF_C2H2	838	858	2.01e1	SMART
ZnF_C2H2	868	888	1.91e1	SMART
low complexity region	946	959	N/A	INTRINSIC
ZnF_C2H2	986	1006	2.7e2	SMART
low complexity region	1008	1021	N/A	INTRINSIC
ZnF_C2H2	1064	1084	3.56e1	SMART
ZnF_C2H2	1094	1117	4.98e-1	SMART
ZnF_C2H2	1130	1153	2.49e-1	SMART
ZnF_C2H2	1159	1181	1.03e-2	SMART
ZnF_C2H2	1187	1210	5.72e-1	SMART
ZnF_C2H2	1216	1239	6.75e0	SMART
ZnF_C2H2	1245	1268	9.58e-3	SMART
ZnF_C2H2	1287	1310	1.64e-1	SMART
ZnF_C2H2	1313	1335	1.1e-2	SMART
ZnF_C2H2	1339	1362	4.05e-1	SMART
ZnF_C2H2	1379	1401	4.45e0	SMART
ZnF_C2H2	1407	1429	8.81e-2	SMART
ZnF_C2H2	1435	1457	1.18e-2	SMART
ZnF_C2H2	1463	1485	1.2e-3	SMART
ZnF_C2H2	1491	1514	3.89e-3	SMART
ZnF_C2H2	1520	1542	4.54e-4	SMART
ZnF_C2H2	1548	1570	1.18e-2	SMART
ZnF_C2H2	1576	1598	3.52e-1	SMART
low complexity region	1628	1637	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000067916

SMART Domains

Protein: ENSMUSP00000064413
Gene: ENSMUSG00000026173

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC
PLCYc	506	622	1.26e-75	SMART
C2	641	747	5.68e-20	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000113737

SMART Domains

Protein: ENSMUSP00000109366
Gene: ENSMUSG00000026135

Domain	Start	End	E-Value	Type
ZnF_C2H2	93	115	9.81e1	SMART
ZnF_C2H2	120	144	2.29e0	SMART
ZnF_C2H2	147	169	9.96e-1	SMART
low complexity region	214	226	N/A	INTRINSIC
ZnF_C2H2	260	282	9.96e-1	SMART
ZnF_C2H2	288	308	1.26e1	SMART
ZnF_C2H2	316	339	1.47e-3	SMART
ZnF_C2H2	350	372	2.75e-3	SMART
ZnF_C2H2	383	408	3.34e-2	SMART
ZnF_C2H2	413	437	4.81e0	SMART
ZnF_C2H2	440	463	7.05e-1	SMART
ZnF_C2H2	469	492	2.17e-1	SMART
ZnF_C2H2	498	520	1.56e-2	SMART
ZnF_C2H2	526	548	8.94e-3	SMART
ZnF_C2H2	554	576	1.38e-3	SMART
ZnF_C2H2	582	604	9.44e-2	SMART
ZnF_C2H2	609	632	1.26e-2	SMART
ZnF_C2H2	641	664	1.31e0	SMART
ZnF_C2H2	670	693	2.63e0	SMART
low complexity region	800	816	N/A	INTRINSIC
low complexity region	850	866	N/A	INTRINSIC
low complexity region	893	904	N/A	INTRINSIC
ZnF_C2H2	936	956	2.01e1	SMART
ZnF_C2H2	966	986	1.91e1	SMART
low complexity region	1044	1057	N/A	INTRINSIC
ZnF_C2H2	1084	1104	2.7e2	SMART
low complexity region	1106	1119	N/A	INTRINSIC
ZnF_C2H2	1162	1182	3.56e1	SMART
ZnF_C2H2	1192	1215	4.98e-1	SMART
ZnF_C2H2	1228	1251	2.49e-1	SMART
ZnF_C2H2	1257	1279	1.03e-2	SMART
ZnF_C2H2	1285	1308	5.72e-1	SMART
ZnF_C2H2	1314	1337	6.75e0	SMART
ZnF_C2H2	1343	1366	9.58e-3	SMART
ZnF_C2H2	1385	1408	1.64e-1	SMART
ZnF_C2H2	1411	1433	1.1e-2	SMART
ZnF_C2H2	1437	1460	4.05e-1	SMART
ZnF_C2H2	1477	1499	4.45e0	SMART
ZnF_C2H2	1505	1527	8.81e-2	SMART
ZnF_C2H2	1533	1555	1.18e-2	SMART
ZnF_C2H2	1561	1583	1.2e-3	SMART
ZnF_C2H2	1589	1612	3.89e-3	SMART
ZnF_C2H2	1618	1640	4.54e-4	SMART
ZnF_C2H2	1646	1668	1.18e-2	SMART
ZnF_C2H2	1674	1696	3.52e-1	SMART
low complexity region	1726	1735	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000113747

SMART Domains

Protein: ENSMUSP00000109376
Gene: ENSMUSG00000026173

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC
PLCYc	506	622	1.26e-75	SMART
C2	641	747	5.68e-20	SMART

Predicted Effect

silent
Transcript: ENSMUST00000113749

SMART Domains

Protein: ENSMUSP00000109378
Gene: ENSMUSG00000026173

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC
PLCYc	538	654	1.26e-75	SMART
C2	673	779	5.68e-20	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000113750
AA Change: E767K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000109379
Gene: ENSMUSG00000026173
AA Change: E767K

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC
PLCYc	506	622	1.26e-75	SMART
C2	641	747	7.16e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000127921

Predicted Effect

probably benign
Transcript: ENSMUST00000141412

SMART Domains

Protein: ENSMUSP00000115322
Gene: ENSMUSG00000026173

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000152707

SMART Domains

Protein: ENSMUSP00000121732
Gene: ENSMUSG00000026173

Domain	Start	End	E-Value	Type
PH	17	126	2.6e-12	SMART
EFh	138	166	9.97e-1	SMART
EFh	174	202	1.83e1	SMART
EFh	207	234	4.45e1	SMART
PLCXc	290	435	2.03e-79	SMART
low complexity region	441	459	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000156613

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

99% (71/72)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011]
PHENOTYPE: Males homozygous for a targeted null mutation are subfertile or sterile. Sperm from mutant males fail to initiate the acrosome reaction. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933411K16Rik	C	T	19: 42,041,112 (GRCm39)	A81V	probably benign	Het
Abcc9	A	T	6: 142,634,853 (GRCm39)	L137*	probably null	Het
Akap10	A	G	11: 61,777,524 (GRCm39)		probably null	Het
Alms1	T	A	6: 85,601,604 (GRCm39)	Y1683N	probably damaging	Het
Atl1	A	G	12: 69,978,408 (GRCm39)	S127G	probably damaging	Het
Cadm1	G	A	9: 47,710,708 (GRCm39)	V204M	probably damaging	Het
Cbr3	T	A	16: 93,487,438 (GRCm39)	I207N	probably damaging	Het
Ccdc148	A	T	2: 58,717,579 (GRCm39)	Y475N	probably damaging	Het
Ccdc33	A	T	9: 57,988,167 (GRCm39)	S144T	probably benign	Het
Ceacam5	C	A	7: 17,479,462 (GRCm39)	A193E	probably benign	Het
Chd2	T	C	7: 73,119,418 (GRCm39)	D1042G	probably benign	Het
Chmp5	A	T	4: 40,952,217 (GRCm39)		probably null	Het
Clca3a1	T	A	3: 144,717,567 (GRCm39)	N467I	probably damaging	Het
D130040H23Rik	T	C	8: 69,754,931 (GRCm39)	V112A	probably damaging	Het
Dbh	A	T	2: 27,058,318 (GRCm39)	D162V	probably damaging	Het
Dnah12	G	A	14: 26,500,869 (GRCm39)		probably null	Het
Dnm2	C	T	9: 21,385,862 (GRCm39)	T295I	probably benign	Het
Faiml	A	C	9: 99,116,446 (GRCm39)	D81E	probably benign	Het
Fer1l4	T	A	2: 155,886,477 (GRCm39)	Y720F	probably benign	Het
Fn1	A	C	1: 71,639,697 (GRCm39)		probably benign	Het
Ftsj3	T	C	11: 106,143,123 (GRCm39)	E397G	probably damaging	Het
Gm16506	T	C	14: 43,964,817 (GRCm39)	K42E		Het
Gpr179	A	G	11: 97,225,556 (GRCm39)	S2200P	probably benign	Het
Gusb	T	C	5: 130,028,884 (GRCm39)	D202G	probably benign	Het
Hspe1	A	G	1: 55,128,310 (GRCm39)	E35G	probably damaging	Het
Kptn	T	A	7: 15,857,023 (GRCm39)	H170Q	probably damaging	Het
Lama3	G	A	18: 12,595,839 (GRCm39)	A923T	probably benign	Het
Lmo7	T	A	14: 102,124,471 (GRCm39)	I432K	probably damaging	Het
Maneal	G	A	4: 124,750,905 (GRCm39)	P284S	probably benign	Het
Mrgbp	A	G	2: 180,224,682 (GRCm39)	T28A	probably benign	Het
Myo1h	T	C	5: 114,476,290 (GRCm39)	V493A		Het
Naxd	T	C	8: 11,556,745 (GRCm39)	L122P	probably damaging	Het
Neto2	T	C	8: 86,397,020 (GRCm39)		probably null	Het
Obscn	T	A	11: 58,904,078 (GRCm39)	R7299*	probably null	Het
Odf2l	G	A	3: 144,845,581 (GRCm39)	V363I	possibly damaging	Het
Or2g25	T	C	17: 37,970,699 (GRCm39)	H175R	probably damaging	Het
Or2t35	A	G	14: 14,407,998 (GRCm38)	T257A	possibly damaging	Het
Or4c100	A	T	2: 88,356,170 (GRCm39)	D81V	probably damaging	Het
Or52a5b	A	T	7: 103,416,940 (GRCm39)	Y221*	probably null	Het
Or5b105	T	A	19: 13,080,537 (GRCm39)	I44F	probably benign	Het
Otud3	G	A	4: 138,624,067 (GRCm39)	P325L	probably benign	Het
Palb2	T	C	7: 121,724,057 (GRCm39)	N564S	probably benign	Het
Pcnt	A	G	10: 76,263,761 (GRCm39)	V401A	possibly damaging	Het
Ppp3ca	T	C	3: 136,574,387 (GRCm39)	F95S	probably damaging	Het
Prkar2a	A	G	9: 108,569,821 (GRCm39)	T56A	probably benign	Het
Psma2	T	A	13: 14,799,815 (GRCm39)	D186E	probably benign	Het
Psmd11	T	A	11: 80,329,099 (GRCm39)	Y72*	probably null	Het
Ror1	G	A	4: 100,160,142 (GRCm39)	D53N	probably benign	Het
Rubcn	C	T	16: 32,656,839 (GRCm39)	R527Q	probably damaging	Het
Sgca	G	A	11: 94,860,373 (GRCm39)	P255S	possibly damaging	Het
Skint11	A	T	4: 114,084,993 (GRCm39)	R167S	probably benign	Het
Slco5a1	A	G	1: 13,060,661 (GRCm39)	V20A	probably benign	Het
Snai2	T	C	16: 14,524,970 (GRCm39)	S159P	probably benign	Het
Taar2	T	G	10: 23,816,725 (GRCm39)	S88R	probably damaging	Het
Tacc3	T	A	5: 33,824,509 (GRCm39)	N378K	possibly damaging	Het
Tek	A	G	4: 94,699,647 (GRCm39)	K342E	probably benign	Het
Tm9sf3	G	T	19: 41,233,944 (GRCm39)	S198*	probably null	Het
Tmbim4	T	A	10: 120,051,514 (GRCm39)	F56I	possibly damaging	Het
Tsen34	T	A	7: 3,697,986 (GRCm39)	S85T	probably benign	Het
Ttc27	C	A	17: 75,054,710 (GRCm39)	Q339K	probably benign	Het
Ubap2	G	T	4: 41,205,550 (GRCm39)	P636T	probably benign	Het
Ubr1	G	A	2: 120,705,979 (GRCm39)	L1495F	probably benign	Het
Vsig10	T	C	5: 117,481,967 (GRCm39)	S386P	probably damaging	Het
Wnk2	C	A	13: 49,300,653 (GRCm39)	R19L	probably benign	Het
Wsb2	T	C	5: 117,508,944 (GRCm39)	L126P	probably damaging	Het
Xylb	A	G	9: 119,211,358 (GRCm39)	I402V	probably benign	Het
Yrdc	A	G	4: 124,744,748 (GRCm39)	S61G	probably benign	Het
Zbtb3	A	G	19: 8,780,771 (GRCm39)	D128G	probably damaging	Het
Zfp423	C	T	8: 88,507,489 (GRCm39)	G952R	probably damaging	Het
Zfp646	A	G	7: 127,478,944 (GRCm39)	T374A	possibly damaging	Het

Other mutations in Plcd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00489:Plcd4	APN	1	74,591,274 (GRCm39)	missense	probably damaging	1.00
IGL01806:Plcd4	APN	1	74,591,192 (GRCm39)	missense	probably benign	0.42
IGL02537:Plcd4	APN	1	74,595,209 (GRCm39)	missense	possibly damaging	0.55
IGL02574:Plcd4	APN	1	74,603,539 (GRCm39)	missense	probably damaging	1.00
IGL02812:Plcd4	APN	1	74,596,967 (GRCm39)	missense	probably damaging	1.00
IGL03350:Plcd4	APN	1	74,588,460 (GRCm39)	missense	probably damaging	1.00
R0415:Plcd4	UTSW	1	74,591,256 (GRCm39)	missense	probably damaging	0.99
R1699:Plcd4	UTSW	1	74,587,394 (GRCm39)	missense	probably benign	0.01
R1852:Plcd4	UTSW	1	74,588,520 (GRCm39)	missense	possibly damaging	0.92
R2120:Plcd4	UTSW	1	74,603,584 (GRCm39)	missense	probably benign	0.00
R2125:Plcd4	UTSW	1	74,604,311 (GRCm39)	missense	probably damaging	1.00
R3023:Plcd4	UTSW	1	74,587,351 (GRCm39)	missense	probably damaging	1.00
R3157:Plcd4	UTSW	1	74,590,313 (GRCm39)	splice site	probably null
R4535:Plcd4	UTSW	1	74,602,627 (GRCm39)	missense	probably damaging	1.00
R4581:Plcd4	UTSW	1	74,587,383 (GRCm39)	missense	probably damaging	1.00
R4863:Plcd4	UTSW	1	74,604,961 (GRCm39)	splice site	probably null
R4911:Plcd4	UTSW	1	74,603,572 (GRCm39)	missense	possibly damaging	0.78
R4987:Plcd4	UTSW	1	74,587,118 (GRCm39)	unclassified	probably benign
R5102:Plcd4	UTSW	1	74,604,313 (GRCm39)	missense	probably damaging	1.00
R5752:Plcd4	UTSW	1	74,587,131 (GRCm39)	splice site	probably null
R5887:Plcd4	UTSW	1	74,590,249 (GRCm39)	missense	probably damaging	1.00
R6318:Plcd4	UTSW	1	74,602,753 (GRCm39)	missense	possibly damaging	0.61
R6648:Plcd4	UTSW	1	74,591,172 (GRCm39)	missense	probably benign	0.00
R6796:Plcd4	UTSW	1	74,601,229 (GRCm39)	missense	probably benign	0.03
R6920:Plcd4	UTSW	1	74,604,994 (GRCm39)	unclassified	probably benign
R7047:Plcd4	UTSW	1	74,591,148 (GRCm39)	missense	possibly damaging	0.87
R7134:Plcd4	UTSW	1	74,593,662 (GRCm39)	missense	probably benign	0.05
R7270:Plcd4	UTSW	1	74,593,838 (GRCm39)	missense	possibly damaging	0.89
R7344:Plcd4	UTSW	1	74,593,811 (GRCm39)	missense	probably damaging	1.00
R7363:Plcd4	UTSW	1	74,590,231 (GRCm39)	missense	probably null	0.99
R7749:Plcd4	UTSW	1	74,604,292 (GRCm39)	missense	possibly damaging	0.60
R7980:Plcd4	UTSW	1	74,604,464 (GRCm39)	missense	probably benign	0.00
R8240:Plcd4	UTSW	1	74,593,660 (GRCm39)	missense	probably benign	0.38
R8762:Plcd4	UTSW	1	74,591,213 (GRCm39)	missense	possibly damaging	0.71
R9000:Plcd4	UTSW	1	74,601,024 (GRCm39)	nonsense	probably null
R9114:Plcd4	UTSW	1	74,591,307 (GRCm39)	missense	possibly damaging	0.60
R9162:Plcd4	UTSW	1	74,601,362 (GRCm39)	missense	probably benign	0.00
R9252:Plcd4	UTSW	1	74,588,359 (GRCm39)	missense	probably damaging	0.97
R9579:Plcd4	UTSW	1	74,596,948 (GRCm39)	missense	probably benign	0.00
Z1176:Plcd4	UTSW	1	74,596,951 (GRCm39)	missense	probably damaging	1.00
Z1176:Plcd4	UTSW	1	74,587,285 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- CAAACCTTGGACTCTTGGCC -3'
(R):5'- TCTCACATAAGAGTGCACAAAAGG -3'

Sequencing Primer
(F):5'- TGGCCAATATCCCAACTGTGG -3'
(R):5'- GAGCAGCTCACGCCTTTAATG -3'

Posted On

2019-05-15