Mutagenetix > Incidental Mutation

Incidental Mutation 'R7137:Crem'

553157

Institutional Source

Beutler Lab

Gene Symbol

Crem

Ensembl Gene

ENSMUSG00000063889

Gene Name

cAMP responsive element modulator

Synonyms

ICER

MMRRC Submission

045248-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.416) question?

Stock #

R7137 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

3266048-3337748 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 3273459 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Serine at position 245 (A245S)

Ref Sequence

ENSEMBL: ENSMUSP00000121118 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025069] [ENSMUST00000049942] [ENSMUST00000082141] [ENSMUST00000122958] [ENSMUST00000123672] [ENSMUST00000124747] [ENSMUST00000126578] [ENSMUST00000129435] [ENSMUST00000130455] [ENSMUST00000130599] [ENSMUST00000131899] [ENSMUST00000134027] [ENSMUST00000136961] [ENSMUST00000137568] [ENSMUST00000139537] [ENSMUST00000140332] [ENSMUST00000144496] [ENSMUST00000146265] [ENSMUST00000147138] [ENSMUST00000148305] [ENSMUST00000150235] [ENSMUST00000151084] [ENSMUST00000151311] [ENSMUST00000152108] [ENSMUST00000152900] [ENSMUST00000154135] [ENSMUST00000154470] [ENSMUST00000154705] [ENSMUST00000154715] [ENSMUST00000156234] [ENSMUST00000165086]

AlphaFold

P27699

Predicted Effect

possibly damaging
Transcript: ENSMUST00000025069
AA Change: A286S

PolyPhen 2 Score 0.642 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000025069
Gene: ENSMUSG00000063889
AA Change: A286S

Domain	Start	End	E-Value	Type
low complexity region	77	88	N/A	INTRINSIC
Pfam:pKID	112	153	3.1e-20	PFAM
BRLZ	285	343	3.8e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000049942
AA Change: A60S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000061925
Gene: ENSMUSG00000063889
AA Change: A60S

Domain	Start	End	E-Value	Type
BRLZ	59	117	3.8e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000082141
AA Change: A249S

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000080780
Gene: ENSMUSG00000063889
AA Change: A249S

Domain	Start	End	E-Value	Type
Pfam:pKID	63	104	2.6e-20	PFAM
BRLZ	248	306	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000122958
AA Change: A245S

PolyPhen 2 Score 0.889 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000121118
Gene: ENSMUSG00000063889
AA Change: A245S

Domain	Start	End	E-Value	Type
low complexity region	24	35	N/A	INTRINSIC
Pfam:pKID	59	100	7.8e-21	PFAM
BRLZ	244	301	2.73e-7	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000123672
AA Change: A158S

PolyPhen 2 Score 0.950 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000120557
Gene: ENSMUSG00000063889
AA Change: A158S

Domain	Start	End	E-Value	Type
Pfam:pKID	47	88	1.5e-20	PFAM
BRLZ	157	214	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000124747
AA Change: A43S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000121352
Gene: ENSMUSG00000063889
AA Change: A43S

Domain	Start	End	E-Value	Type
BRLZ	42	100	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000126578
AA Change: A245S

PolyPhen 2 Score 0.824 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000114780
Gene: ENSMUSG00000063889
AA Change: A245S

Domain	Start	End	E-Value	Type
low complexity region	24	35	N/A	INTRINSIC
Pfam:pKID	59	100	7.8e-21	PFAM
BRLZ	244	302	3.8e-6	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000118649
Gene: ENSMUSG00000063889
AA Change: A271S

Domain	Start	End	E-Value	Type
low complexity region	63	74	N/A	INTRINSIC
Pfam:pKID	98	139	3.1e-21	PFAM
BRLZ	271	328	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000129435
AA Change: A184S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000117438
Gene: ENSMUSG00000063889
AA Change: A184S

Domain	Start	End	E-Value	Type
Pfam:pKID	10	51	5.6e-21	PFAM
BRLZ	183	240	2.73e-7	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130455
AA Change: A276S

PolyPhen 2 Score 0.837 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000121541
Gene: ENSMUSG00000063889
AA Change: A276S

Domain	Start	End	E-Value	Type
low complexity region	67	78	N/A	INTRINSIC
Pfam:pKID	102	143	2.8e-20	PFAM
BRLZ	275	333	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130599
AA Change: A170S

PolyPhen 2 Score 0.890 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000115471
Gene: ENSMUSG00000063889
AA Change: A170S

Domain	Start	End	E-Value	Type
Pfam:pKID	47	88	1.6e-20	PFAM
BRLZ	169	226	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000131899
AA Change: A225S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000119353
Gene: ENSMUSG00000063889
AA Change: A225S

Domain	Start	End	E-Value	Type
low complexity region	67	78	N/A	INTRINSIC
Pfam:pKID	102	143	2.2e-20	PFAM
BRLZ	224	282	3.8e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000134027

Predicted Effect

probably damaging
Transcript: ENSMUST00000136961
AA Change: A209S

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000115363
Gene: ENSMUSG00000063889
AA Change: A209S

Domain	Start	End	E-Value	Type
Pfam:pKID	35	76	6.5e-21	PFAM
BRLZ	208	265	2.73e-7	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000137568
AA Change: A164S

PolyPhen 2 Score 0.658 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000115336
Gene: ENSMUSG00000063889
AA Change: A164S

Domain	Start	End	E-Value	Type
Pfam:pKID	53	94	1.5e-20	PFAM
BRLZ	163	221	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000139537
AA Change: A36S

PolyPhen 2 Score 0.658 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000122123
Gene: ENSMUSG00000063889
AA Change: A36S

Domain	Start	End	E-Value	Type
BRLZ	35	93	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000140332
AA Change: A49S

PolyPhen 2 Score 0.945 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000115423
Gene: ENSMUSG00000063889
AA Change: A49S

Domain	Start	End	E-Value	Type
BRLZ	48	105	2.73e-7	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000144496
AA Change: A221S

PolyPhen 2 Score 0.820 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000120349
Gene: ENSMUSG00000063889
AA Change: A221S

Domain	Start	End	E-Value	Type
Pfam:pKID	35	76	6.9e-21	PFAM
BRLZ	220	277	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000146265
AA Change: A213S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000119638
Gene: ENSMUSG00000063889
AA Change: A213S

Domain	Start	End	E-Value	Type
low complexity region	67	78	N/A	INTRINSIC
Pfam:pKID	102	143	2.1e-20	PFAM
BRLZ	212	269	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000147138
AA Change: A61S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000114280
Gene: ENSMUSG00000063889
AA Change: A61S

Domain	Start	End	E-Value	Type
BRLZ	60	117	2.73e-7	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000148305

Predicted Effect

probably damaging
Transcript: ENSMUST00000150235
AA Change: A298S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000121233
Gene: ENSMUSG00000063889
AA Change: A298S

Domain	Start	End	E-Value	Type
low complexity region	77	88	N/A	INTRINSIC
Pfam:pKID	112	153	1.1e-20	PFAM
BRLZ	297	354	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000151084
AA Change: A54S

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000118578
Gene: ENSMUSG00000063889
AA Change: A54S

Domain	Start	End	E-Value	Type
BRLZ	48	106	3.8e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000151311
AA Change: A237S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000118267
Gene: ENSMUSG00000063889
AA Change: A237S

Domain	Start	End	E-Value	Type
Pfam:pKID	63	104	7.5e-21	PFAM
BRLZ	236	293	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000152108
AA Change: A158S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000122241
Gene: ENSMUSG00000063889
AA Change: A158S

Domain	Start	End	E-Value	Type
Pfam:pKID	35	76	4.7e-21	PFAM
BRLZ	157	214	2.73e-7	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000152900
AA Change: A239S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000123515
Gene: ENSMUSG00000063889
AA Change: A239S

Domain	Start	End	E-Value	Type
Pfam:pKID	53	94	2.4e-20	PFAM
BRLZ	238	296	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000154135
AA Change: A186S

PolyPhen 2 Score 0.868 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000122051
Gene: ENSMUSG00000063889
AA Change: A186S

Domain	Start	End	E-Value	Type
Pfam:pKID	63	104	1.9e-20	PFAM
BRLZ	185	243	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000154470
AA Change: A223S

PolyPhen 2 Score 0.907 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000118128
Gene: ENSMUSG00000063889
AA Change: A223S

Domain	Start	End	E-Value	Type
low complexity region	77	88	N/A	INTRINSIC
Pfam:pKID	112	153	2.3e-20	PFAM
BRLZ	222	280	3.8e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000154705
AA Change: A66S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000119194
Gene: ENSMUSG00000063889
AA Change: A66S

Domain	Start	End	E-Value	Type
BRLZ	60	118	3.8e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000154715
AA Change: A270S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000122179
Gene: ENSMUSG00000063889
AA Change: A270S

Domain	Start	End	E-Value	Type
low complexity region	49	60	N/A	INTRINSIC
Pfam:pKID	84	125	8.7e-21	PFAM
BRLZ	269	327	3.8e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000156234
AA Change: A282S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000121388
Gene: ENSMUSG00000063889
AA Change: A282S

Domain	Start	End	E-Value	Type
low complexity region	61	72	N/A	INTRINSIC
Pfam:pKID	96	137	9.1e-21	PFAM
BRLZ	281	339	3.8e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165086
AA Change: A235S

PolyPhen 2 Score 0.882 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000127353
Gene: ENSMUSG00000063889
AA Change: A235S

Domain	Start	End	E-Value	Type
low complexity region	77	88	N/A	INTRINSIC
Pfam:pKID	112	153	2.4e-20	PFAM
BRLZ	234	292	3.8e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters, where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes, including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq, Oct 2012]
PHENOTYPE: Homozygotes for targeted mutations exhibit reduced regenerative capacity after partial hepatectomy and reduced cardiac function. Males are sterile due to a block in spermiogenesis associated with a lack of postmeiotic gene expression. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	T	C	6: 121,654,944 (GRCm39)	S1423P	possibly damaging	Het
Acvr1c	A	G	2: 58,173,399 (GRCm39)		probably null	Het
Adgrf5	A	G	17: 43,761,788 (GRCm39)	K1161R	probably damaging	Het
Aoc1l3	A	G	6: 48,967,083 (GRCm39)	Y677C	probably damaging	Het
Arhgap32	T	G	9: 32,063,232 (GRCm39)	D80E	probably benign	Het
Aspg	T	C	12: 112,078,632 (GRCm39)	V30A	possibly damaging	Het
Bcl2a1d	T	C	9: 88,613,531 (GRCm39)	D81G	probably damaging	Het
Cep170b	T	C	12: 112,701,601 (GRCm39)	V160A	probably benign	Het
Ces1c	T	C	8: 93,857,470 (GRCm39)	Y37C	probably benign	Het
Cntnap5a	T	C	1: 116,017,106 (GRCm39)	L233P	probably damaging	Het
Cyp2d12	C	T	15: 82,442,022 (GRCm39)	A280V	probably benign	Het
Dnah2	C	T	11: 69,382,381 (GRCm39)	G1243D	probably damaging	Het
Eif1ad19	A	T	12: 87,740,316 (GRCm39)	L81Q	possibly damaging	Het
Emcn	T	G	3: 137,109,752 (GRCm39)	N131K	probably damaging	Het
Fat3	A	T	9: 15,908,444 (GRCm39)	D2519E	probably damaging	Het
Fibin	T	A	2: 110,193,001 (GRCm39)	D47V	probably damaging	Het
Fsd1	G	A	17: 56,300,876 (GRCm39)	R245H	probably damaging	Het
Gimap8	C	A	6: 48,627,187 (GRCm39)	L54I	probably damaging	Het
Gm20481	A	G	17: 35,189,071 (GRCm39)	Y27C	unknown	Het
Grin1	T	G	2: 25,203,550 (GRCm39)	M154L	probably benign	Het
Ighv8-13	A	G	12: 115,729,197 (GRCm39)	L20P	probably damaging	Het
Insc	G	A	7: 114,410,850 (GRCm39)	V236I	probably benign	Het
Lrrk1	A	G	7: 65,935,027 (GRCm39)	F1031L	probably benign	Het
Man2a2	C	A	7: 80,009,499 (GRCm39)	R785L	probably benign	Het
Mcur1	C	A	13: 43,697,931 (GRCm39)		probably null	Het
Med12l	A	C	3: 59,165,675 (GRCm39)	R1464S	probably damaging	Het
Mycbp2	A	T	14: 103,520,115 (GRCm39)	M767K	possibly damaging	Het
Naalad2	T	C	9: 18,234,783 (GRCm39)	I762V	probably benign	Het
Nfkbid	A	G	7: 30,125,681 (GRCm39)	T357A	possibly damaging	Het
Pcdh17	A	G	14: 84,770,989 (GRCm39)	R1156G	possibly damaging	Het
Pcdhb1	T	C	18: 37,400,445 (GRCm39)	S799P	possibly damaging	Het
Pde3a	A	T	6: 141,444,472 (GRCm39)	E1093D	probably benign	Het
Plcg1	T	C	2: 160,595,846 (GRCm39)	Y572H	possibly damaging	Het
Plxna4	A	G	6: 32,494,199 (GRCm39)	L139P	probably damaging	Het
Psma1	A	G	7: 113,873,683 (GRCm39)	Y6H	probably damaging	Het
Psmd2	A	G	16: 20,471,377 (GRCm39)	E76G	probably benign	Het
Ptk2	G	A	15: 73,093,658 (GRCm39)	P854S	possibly damaging	Het
Pyy	T	G	11: 101,998,099 (GRCm39)	D27A	possibly damaging	Het
Slc22a2	A	G	17: 12,803,228 (GRCm39)	T21A	probably benign	Het
Slc25a2	T	C	18: 37,771,200 (GRCm39)	I110V	probably benign	Het
Sult1c2	A	C	17: 54,145,422 (GRCm39)	W85G	probably damaging	Het
Syt12	C	T	19: 4,503,978 (GRCm39)	D218N	probably damaging	Het
Tln1	C	T	4: 43,540,616 (GRCm39)	V1462M	probably damaging	Het
Tor1aip2	T	A	1: 155,927,722 (GRCm39)	N33K	possibly damaging	Het
Usp17lb	C	A	7: 104,490,798 (GRCm39)	W43L	probably benign	Het
Vmn1r76	A	G	7: 11,664,612 (GRCm39)	Y201H	possibly damaging	Het
Wnk1	C	A	6: 120,015,173 (GRCm39)		probably benign	Het
Wrnip1	A	G	13: 32,986,732 (GRCm39)	D171G	probably benign	Het
Zar1	T	A	5: 72,738,159 (GRCm39)	N81I	probably damaging	Het
Zbtb45	T	C	7: 12,741,083 (GRCm39)	T392A	probably benign	Het
Zfp445	T	C	9: 122,683,843 (GRCm39)	E272G	probably damaging	Het

Other mutations in Crem

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01085:Crem	APN	18	3,299,236 (GRCm39)	missense	probably damaging	1.00
IGL01532:Crem	APN	18	3,276,732 (GRCm39)	missense	probably benign	0.02
IGL02500:Crem	APN	18	3,273,477 (GRCm39)	missense	probably damaging	1.00
IGL03280:Crem	APN	18	3,273,415 (GRCm39)	splice site	probably benign
menthe	UTSW	18	3,268,070 (GRCm39)	missense	probably damaging	1.00
R0379:Crem	UTSW	18	3,299,226 (GRCm39)	missense	probably damaging	1.00
R0987:Crem	UTSW	18	3,288,060 (GRCm39)	missense	probably damaging	0.98
R1829:Crem	UTSW	18	3,295,037 (GRCm39)	splice site	probably null
R1932:Crem	UTSW	18	3,299,284 (GRCm39)	missense	probably benign	0.27
R2086:Crem	UTSW	18	3,288,098 (GRCm39)	intron	probably benign
R2093:Crem	UTSW	18	3,299,256 (GRCm39)	missense	probably damaging	1.00
R4152:Crem	UTSW	18	3,288,055 (GRCm39)	missense	probably damaging	0.99
R4568:Crem	UTSW	18	3,299,175 (GRCm39)	missense	probably damaging	0.98
R4758:Crem	UTSW	18	3,327,527 (GRCm39)	missense	probably damaging	1.00
R6032:Crem	UTSW	18	3,267,673 (GRCm39)	missense	probably damaging	1.00
R6032:Crem	UTSW	18	3,267,673 (GRCm39)	missense	probably damaging	1.00
R6445:Crem	UTSW	18	3,309,671 (GRCm39)	missense	probably benign
R6525:Crem	UTSW	18	3,268,070 (GRCm39)	missense	probably damaging	1.00
R6651:Crem	UTSW	18	3,325,428 (GRCm39)	missense	probably benign	0.18
R7035:Crem	UTSW	18	3,327,503 (GRCm39)	missense	probably damaging	1.00
R7401:Crem	UTSW	18	3,295,329 (GRCm39)	missense	probably damaging	1.00
R7463:Crem	UTSW	18	3,295,094 (GRCm39)	missense	probably benign	0.06
R7516:Crem	UTSW	18	3,299,141 (GRCm39)	splice site	probably null
R8095:Crem	UTSW	18	3,295,106 (GRCm39)	missense	probably benign	0.00
R8146:Crem	UTSW	18	3,288,007 (GRCm39)	missense	possibly damaging	0.68
R8266:Crem	UTSW	18	3,309,535 (GRCm39)	intron	probably benign
R8308:Crem	UTSW	18	3,295,397 (GRCm39)	missense	possibly damaging	0.55
R8825:Crem	UTSW	18	3,268,061 (GRCm39)	missense	probably damaging	0.99
R8899:Crem	UTSW	18	3,295,370 (GRCm39)	missense	probably damaging	0.99
R8976:Crem	UTSW	18	3,268,088 (GRCm39)	missense	possibly damaging	0.88
R9681:Crem	UTSW	18	3,268,067 (GRCm39)	missense	probably damaging	1.00
Z1176:Crem	UTSW	18	3,267,730 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATGGGCAGAGCATAAGCAC -3'
(R):5'- GGGAACAGGCTAGAGTATTTGTAGC -3'

Sequencing Primer
(F):5'- GAGCATAAGCACACACACGAAG -3'
(R):5'- ACAATCTTTACTGACATGTACAAGG -3'

Posted On

2019-05-15