Incidental Mutation 'B5639:Fam237b'
ID 554
Institutional Source Beutler Lab
Gene Symbol Fam237b
Ensembl Gene ENSMUSG00000073234
Gene Name family with sequence similarity 237, member B
Synonyms Gm8773, Gm29287
Accession Numbers
Essential gene? Probably non essential (E-score: 0.194) question?
Stock # B5639 of strain 3d
Quality Score
Status Validated
Chromosome 5
Chromosomal Location 5624004-5626205 bp(+) (GRCm39)
Type of Mutation utr 5 prime
DNA Base Change (assembly) C to T at 5624060 bp (GRCm39)
Zygosity Homozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000147824 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000101627]
AlphaFold Q3UQ24
Predicted Effect probably benign
Transcript: ENSMUST00000101627
SMART Domains Protein: ENSMUSP00000099149
Gene: ENSMUSG00000073234

DomainStartEndE-ValueType
signal peptide 1 24 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000101627
Coding Region Coverage
  • 1x: 89.7%
  • 3x: 78.3%
Het Detection Efficiency 55.9%
Validation Efficiency 83% (206/248)
Allele List at MGI
Other mutations in this stock
Total: 15 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Dnmt1 G A 9: 20,819,264 (GRCm39) probably benign Het
Eno1 A G 4: 150,329,569 (GRCm39) probably benign Het
Ercc8 G A 13: 108,297,257 (GRCm39) G56R probably damaging Homo
Idh1 A G 1: 65,204,257 (GRCm39) probably null Homo
Incenp G A 19: 9,871,182 (GRCm39) T149I unknown Het
Or5d16 G A 2: 87,773,942 (GRCm39) S10F probably benign Het
Or5k17 A T 16: 58,746,889 (GRCm39) I15K probably benign Homo
Pdk2 T C 11: 94,923,324 (GRCm39) D100G possibly damaging Homo
Prss56 T C 1: 87,114,892 (GRCm39) L465P probably benign Homo
Slc10a3 G A X: 73,413,145 (GRCm39) P416L probably damaging Homo
Syne2 C A 12: 75,976,564 (GRCm39) T1243K probably benign Het
Vwf T C 6: 125,619,947 (GRCm39) Y1542H probably damaging Homo
Zc3h13 G A 14: 75,553,479 (GRCm39) R302Q probably damaging Het
Zfhx4 G T 3: 5,468,235 (GRCm39) G2798W probably damaging Homo
Zfp667 A G 7: 6,293,544 (GRCm39) T15A probably damaging Het
Other mutations in Fam237b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01572:Fam237b APN 5 5,625,475 (GRCm39) missense probably damaging 0.97
IGL03040:Fam237b APN 5 5,625,566 (GRCm39) nonsense probably null
R0098:Fam237b UTSW 5 5,625,355 (GRCm39) missense possibly damaging 0.68
R0972:Fam237b UTSW 5 5,625,512 (GRCm39) missense probably benign 0.09
R1789:Fam237b UTSW 5 5,625,652 (GRCm39) missense possibly damaging 0.82
R4952:Fam237b UTSW 5 5,625,387 (GRCm39) missense probably benign 0.02
R5229:Fam237b UTSW 5 5,625,565 (GRCm39) missense possibly damaging 0.94
R8881:Fam237b UTSW 5 5,625,379 (GRCm39) missense possibly damaging 0.93
R9649:Fam237b UTSW 5 5,625,549 (GRCm39) nonsense probably null
Nature of Mutation

DNA sequencing using the SOLiD technique identified a C to T transition at position 262 of the Gm8773 transcript in exon 1 of 2 total exons.  The mutated nucleotide introduces a premature stop codon at glutamine 63 of the encoded protein. The mutation has been confirmed by DNA sequencing using the Sanger method (see trace files for B5639).

Protein Function and Prediction

The Gm8773 gene is predicted to encode a 132 amino acid protein with no recognized domains.

The premature stop introduced by the 3d mutation in the Gm8773 gene truncates 70 amino acids from the C-terminus of the protein. This allele is likely null.

Posted On 2010-11-19