Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4458001:Prp2'

555909

Institutional Source

Beutler Lab

Gene Symbol

Prp2

Ensembl Gene

ENSMUSG00000058295

Gene Name

proline rich protein 2

Synonyms

MP14

Accession Numbers

Essential gene?

Not available question?

Stock #

PIT4458001 (G1)

Quality Score

176.009

Status

Not validated

Chromosome

Chromosomal Location

132572874-132577665 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 132577510 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 266 (P266S)

Ref Sequence

ENSEMBL: ENSMUSP00000135942 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076061] [ENSMUST00000178961]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000076061
AA Change: P266S

SMART Domains

Protein: ENSMUSP00000075435
Gene: ENSMUSG00000058295
AA Change: P266S

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
low complexity region	24	44	N/A	INTRINSIC
low complexity region	49	299	N/A	INTRINSIC
low complexity region	302	316	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000178961
AA Change: P266S

SMART Domains

Protein: ENSMUSP00000135942
Gene: ENSMUSG00000058295
AA Change: P266S

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
low complexity region	24	44	N/A	INTRINSIC
low complexity region	49	299	N/A	INTRINSIC
low complexity region	302	316	N/A	INTRINSIC

Coding Region Coverage

1x: 93.1%
3x: 90.8%
10x: 85.3%
20x: 73.0%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the heterogeneous family of proline-rich salivary glycoproteins. The expression of this gene is dramatically induced in the parotid and submandibular glands of mice by beta-adrenergic stimulation. The encoded protein serves an important dental function by protecting mice against toxic dietary polyphenols such as tannins and influence the visco-elastic properties of the mucus. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 6. [provided by RefSeq, Nov 2015]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	G	T	11: 9,248,304 (GRCm39)	V2684L	probably benign	Het
Adamts9	T	A	6: 92,866,886 (GRCm39)	I718F	probably damaging	Het
Adcyap1r1	A	G	6: 55,455,067 (GRCm39)	D110G	probably benign	Het
Adgrd1	G	A	5: 129,208,641 (GRCm39)	G281D	probably damaging	Het
Afg1l	A	T	10: 42,330,366 (GRCm39)	C100*	probably null	Het
Atp2a2	G	A	5: 122,595,372 (GRCm39)	Q993*	probably null	Het
Baz1a	A	G	12: 54,977,095 (GRCm39)	M389T	probably benign	Het
Btnl4	T	A	17: 34,693,242 (GRCm39)	M58L	probably benign	Het
Card6	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	15: 5,128,173 (GRCm39)		probably benign	Het
Cpeb1	A	T	7: 80,998,180 (GRCm39)	F533Y	probably damaging	Het
Ctnna1	T	A	18: 35,308,179 (GRCm39)	N166K	possibly damaging	Het
Ecsit	T	C	9: 21,987,580 (GRCm39)	H153R	probably damaging	Het
Gbp8	T	C	5: 105,162,955 (GRCm39)	K480E	probably benign	Het
Glyat	A	T	19: 12,625,373 (GRCm39)	T66S	probably benign	Het
Gm1110	T	C	9: 26,792,124 (GRCm39)	Q632R	probably benign	Het
Gm12185	C	T	11: 48,798,738 (GRCm39)	R585Q	probably damaging	Het
Gm17669	TAA	TAAA	18: 67,695,819 (GRCm39)		probably null	Het
Gm7489	A	C	15: 53,749,195 (GRCm39)	E89A	unknown	Het
Grb7	C	T	11: 98,344,655 (GRCm39)	Q353*	probably null	Het
Ifi207	G	A	1: 173,562,738 (GRCm39)	T136I	unknown	Het
Ighv3-1	T	C	12: 113,928,224 (GRCm39)	Y45C	probably benign	Het
Ing5	A	G	1: 93,739,668 (GRCm39)	M92V	possibly damaging	Het
Ivl	TTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTG	3: 92,479,608 (GRCm39)		probably benign	Het
Kdm6b	A	T	11: 69,290,778 (GRCm39)	D1630E	unknown	Het
Lmo7	C	T	14: 102,124,923 (GRCm39)	Q583*	probably null	Het
Lrrc37a	T	C	11: 103,395,338 (GRCm39)	D29G	probably benign	Het
Mpdz	G	T	4: 81,337,263 (GRCm39)	A10D	probably damaging	Het
Myh4	A	G	11: 67,131,821 (GRCm39)	M94V	possibly damaging	Het
Nectin2	A	C	7: 19,472,252 (GRCm39)	L46V	probably benign	Het
Nynrin	A	T	14: 56,101,425 (GRCm39)	T365S	probably benign	Het
Or4c120	A	T	2: 89,000,977 (GRCm39)	I193K	probably benign	Het
Pde4b	A	C	4: 102,459,875 (GRCm39)	E570A	probably damaging	Het
Phf3	T	C	1: 30,855,622 (GRCm39)	H988R	probably damaging	Het
Ppfia2	A	G	10: 106,763,708 (GRCm39)	K1234E	probably benign	Het
Prl7c1	A	T	13: 27,957,741 (GRCm39)	M233K	probably benign	Het
Ralgds	T	C	2: 28,432,486 (GRCm39)	L160P	probably damaging	Het
Rasa1	A	T	13: 85,375,237 (GRCm39)	M664K	possibly damaging	Het
Ryr2	T	C	13: 11,570,334 (GRCm39)	T4930A	probably benign	Het
Senp8	T	C	9: 59,644,763 (GRCm39)	Y131C	probably damaging	Het
Sp100	A	G	1: 85,635,837 (GRCm39)	I547M	probably benign	Het
Spata20	A	G	11: 94,375,434 (GRCm39)	M120T	probably damaging	Het
Spata31	A	G	13: 65,069,664 (GRCm39)	H604R	probably benign	Het
Sycp1	A	T	3: 102,842,149 (GRCm39)	S53T	probably benign	Het
Tgm1	T	C	14: 55,950,022 (GRCm39)	D62G	unknown	Het
Trpm1	G	C	7: 63,918,309 (GRCm39)	E1434Q	possibly damaging	Het
Tshb	A	T	3: 102,685,480 (GRCm39)	Y50N	probably damaging	Het
Wdr17	A	T	8: 55,126,614 (GRCm39)	Y413*	probably null	Het
Zdhhc16	G	A	19: 41,926,209 (GRCm39)	G55R	possibly damaging	Het

Other mutations in Prp2

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL01383:Prp2	APN	6	132,576,841 (GRCm39)	missense	unknown
IGL02951:Prp2	APN	6	132,576,788 (GRCm39)	missense	unknown
R2034:Prp2	UTSW	6	132,572,947 (GRCm39)	splice site	probably null
R2432:Prp2	UTSW	6	132,576,874 (GRCm39)	missense	unknown
R5283:Prp2	UTSW	6	132,577,606 (GRCm39)	missense	unknown
R7761:Prp2	UTSW	6	132,577,306 (GRCm39)	nonsense	probably null
R7877:Prp2	UTSW	6	132,572,928 (GRCm39)	missense	unknown
R8033:Prp2	UTSW	6	132,577,391 (GRCm39)	missense	unknown
R8062:Prp2	UTSW	6	132,577,651 (GRCm39)	missense	unknown
R8695:Prp2	UTSW	6	132,576,932 (GRCm39)	missense	unknown
R8696:Prp2	UTSW	6	132,577,322 (GRCm39)	missense	unknown
R8938:Prp2	UTSW	6	132,577,581 (GRCm39)	missense	unknown
RF005:Prp2	UTSW	6	132,577,464 (GRCm39)	small deletion	probably benign
RF016:Prp2	UTSW	6	132,577,475 (GRCm39)	small deletion	probably benign
RF039:Prp2	UTSW	6	132,577,464 (GRCm39)	small deletion	probably benign
RF052:Prp2	UTSW	6	132,577,475 (GRCm39)	small deletion	probably benign
RF054:Prp2	UTSW	6	132,577,484 (GRCm39)	frame shift	probably null
RF057:Prp2	UTSW	6	132,577,493 (GRCm39)	frame shift	probably null
X0053:Prp2	UTSW	6	132,577,559 (GRCm39)	missense	unknown
Z1176:Prp2	UTSW	6	132,577,200 (GRCm39)	missense	unknown
Z1177:Prp2	UTSW	6	132,572,933 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GCAGCGATACCCTCAAAGC -3'
(R):5'- CACAAGGGGATAACAATGAGTCA -3'

Sequencing Primer
(F):5'- GCCCACCACCACCAGGAG -3'
(R):5'- AATGAGTCATACCTGTCACGG -3'

Posted On

2019-06-07