Mutagenetix > Incidental Mutation

Incidental Mutation 'R7157:Or10a3'

557375

Institutional Source

Beutler Lab

Gene Symbol

Or10a3

Ensembl Gene

ENSMUSG00000046431

Gene Name

olfactory receptor family 10 subfamily A member 3

Synonyms

Olfr518, MOR268-5, GA_x6K02T2PBJ9-11211854-11210853

MMRRC Submission

045258-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R7157 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

108479810-108480811 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 108480475 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 113 (F113L)

Ref Sequence

ENSEMBL: ENSMUSP00000151883 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059617] [ENSMUST00000217803]

AlphaFold

Q8VEW1

Predicted Effect

probably benign
Transcript: ENSMUST00000059617
AA Change: F113L

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000050503
Gene: ENSMUSG00000046431
AA Change: F113L

Domain	Start	End	E-Value	Type
low complexity region	11	22	N/A	INTRINSIC
Pfam:7tm_4	50	327	7.1e-60	PFAM
Pfam:7tm_1	60	318	1.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217803
AA Change: F113L

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	G	A	7: 41,276,400 (GRCm39)	G701D	probably damaging	Het
6030468B19Rik	T	A	11: 117,693,780 (GRCm39)	N82K	probably damaging	Het
Abca17	A	T	17: 24,554,564 (GRCm39)	V130E	possibly damaging	Het
Alpk2	T	A	18: 65,399,348 (GRCm39)	K2077*	probably null	Het
Aox1	T	C	1: 58,322,651 (GRCm39)	F73S	probably benign	Het
Ap3b1	T	A	13: 94,668,542 (GRCm39)	C965*	probably null	Het
Arhgef10	T	A	8: 14,980,030 (GRCm39)	V90E	probably damaging	Het
Atr	T	A	9: 95,751,953 (GRCm39)	H523Q	probably benign	Het
Bicdl1	G	T	5: 115,789,916 (GRCm39)	Q511K	possibly damaging	Het
Capn15	A	T	17: 26,184,228 (GRCm39)	N150K	probably damaging	Het
Card6	A	T	15: 5,129,591 (GRCm39)	W602R	probably benign	Het
Clnk	A	T	5: 38,927,234 (GRCm39)	S82T	possibly damaging	Het
Cmah	T	C	13: 24,620,612 (GRCm39)	I282T	probably damaging	Het
Cog8	A	G	8: 107,779,131 (GRCm39)	I382T	probably benign	Het
Dhrs7c	G	T	11: 67,700,722 (GRCm39)		probably null	Het
Dhrs9	T	A	2: 69,223,502 (GRCm39)	D83E	probably damaging	Het
Dmac2l	A	T	12: 69,788,562 (GRCm39)	N154Y	probably benign	Het
Dmgdh	G	A	13: 93,852,043 (GRCm39)	G624E	probably damaging	Het
Dnajc30	C	A	5: 135,093,569 (GRCm39)	F155L	probably damaging	Het
Drc7	A	C	8: 95,800,778 (GRCm39)	K600T	probably damaging	Het
Efhb	A	C	17: 53,707,928 (GRCm39)	I745S	probably damaging	Het
Elp1	ACTTCTTCTTCTTCTTCTTCTTC	ACTTCTTCTTCTTCTTCTTC	4: 56,781,176 (GRCm39)		probably benign	Het
Fasn	T	A	11: 120,701,291 (GRCm39)	K1988*	probably null	Het
Gad2	T	C	2: 22,525,035 (GRCm39)	L273P	probably damaging	Het
Gm45861	A	T	8: 28,032,536 (GRCm39)	K887*	probably null	Het
Gm45861	A	T	8: 28,032,537 (GRCm39)	K887I	unknown	Het
Gm9195	A	G	14: 72,718,221 (GRCm39)	Y152H	probably damaging	Het
Grip1	G	A	10: 119,781,061 (GRCm39)	D237N	probably damaging	Het
Hamp	A	C	7: 30,641,961 (GRCm39)	C65G	possibly damaging	Het
Hes3	A	G	4: 152,372,581 (GRCm39)		probably benign	Het
Hgh1	T	C	15: 76,254,650 (GRCm39)	I342T	probably damaging	Het
Hlcs	T	A	16: 94,069,023 (GRCm39)	K66*	probably null	Het
Ift80	A	T	3: 68,898,277 (GRCm39)	C19*	probably null	Het
Kbtbd12	C	T	6: 88,595,650 (GRCm39)	C60Y	probably damaging	Het
Kdm4c	T	A	4: 74,263,804 (GRCm39)	V696E	probably benign	Het
Lect2	A	G	13: 56,690,803 (GRCm39)	I117T	unknown	Het
Lipn	T	A	19: 34,054,390 (GRCm39)	Y209*	probably null	Het
Lpin3	G	A	2: 160,740,627 (GRCm39)	V391I	probably benign	Het
Mocs2	A	T	13: 114,961,143 (GRCm39)	I47L	probably benign	Het
Mogs	T	A	6: 83,095,488 (GRCm39)	H768Q	probably benign	Het
Nbeal1	T	A	1: 60,276,317 (GRCm39)	I686N	probably damaging	Het
Nbeal1	T	A	1: 60,299,793 (GRCm39)	C1376*	probably null	Het
Ninl	A	G	2: 150,791,263 (GRCm39)	Y1087H	possibly damaging	Het
Or10ak14	A	G	4: 118,611,615 (GRCm39)	V42A	possibly damaging	Het
Or4k5	A	G	14: 50,385,689 (GRCm39)	L214S	probably damaging	Het
Or51b6b	A	C	7: 103,309,788 (GRCm39)	L223R	probably damaging	Het
Or6s1	A	G	14: 51,308,616 (GRCm39)	V78A	possibly damaging	Het
Or8k30	T	A	2: 86,339,369 (GRCm39)	C189S	probably damaging	Het
Pkn1	A	G	8: 84,398,363 (GRCm39)	F768S	probably damaging	Het
Plekha2	A	G	8: 25,553,957 (GRCm39)	F82L	probably damaging	Het
Ppie	T	C	4: 123,028,900 (GRCm39)	E111G	probably benign	Het
Rps6ka5	T	C	12: 100,547,679 (GRCm39)	Y277C	probably damaging	Het
Scgb2b12	A	G	7: 32,026,102 (GRCm39)	V30A	probably benign	Het
Serac1	A	T	17: 6,124,476 (GRCm39)	S16T	probably benign	Het
Sf3a3	T	C	4: 124,616,693 (GRCm39)	Y192H	probably damaging	Het
Slc14a1	A	G	18: 78,145,626 (GRCm39)	V436A	probably benign	Het
Smc3	T	A	19: 53,630,329 (GRCm39)	M1112K	probably damaging	Het
Tnk2	G	A	16: 32,499,986 (GRCm39)	G1017E	probably damaging	Het
Tnn	T	A	1: 159,953,947 (GRCm39)	K603*	probably null	Het
Trpm6	T	C	19: 18,815,462 (GRCm39)	S1183P	possibly damaging	Het
Ttn	A	T	2: 76,614,724 (GRCm39)	V16964E	possibly damaging	Het
Usp17le	G	A	7: 104,417,696 (GRCm39)	T482I	probably benign	Het
Vmn1r219	T	A	13: 23,347,525 (GRCm39)	M238K	probably damaging	Het
Wfs1	C	T	5: 37,124,516 (GRCm39)	G792S	probably benign	Het
Xpa	A	T	4: 46,185,612 (GRCm39)	L122Q	probably damaging	Het

Other mutations in Or10a3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02713:Or10a3	APN	7	108,480,060 (GRCm39)	missense	probably damaging	0.99
IGL02995:Or10a3	APN	7	108,480,198 (GRCm39)	missense	probably damaging	1.00
IGL03162:Or10a3	APN	7	108,480,811 (GRCm39)	start codon destroyed	probably null
IGL03389:Or10a3	APN	7	108,479,982 (GRCm39)	missense	probably damaging	0.99
R0731:Or10a3	UTSW	7	108,480,740 (GRCm39)	missense	probably damaging	1.00
R1669:Or10a3	UTSW	7	108,479,920 (GRCm39)	missense	probably benign	0.00
R2235:Or10a3	UTSW	7	108,480,172 (GRCm39)	missense	probably benign	0.09
R4740:Or10a3	UTSW	7	108,480,689 (GRCm39)	missense	probably benign	0.05
R4902:Or10a3	UTSW	7	108,480,624 (GRCm39)	missense	probably benign	0.00
R5343:Or10a3	UTSW	7	108,480,205 (GRCm39)	missense	possibly damaging	0.87
R6744:Or10a3	UTSW	7	108,480,037 (GRCm39)	missense	probably damaging	0.99
R7326:Or10a3	UTSW	7	108,480,023 (GRCm39)	missense	probably damaging	1.00
R7713:Or10a3	UTSW	7	108,479,889 (GRCm39)	missense	probably damaging	1.00
R7819:Or10a3	UTSW	7	108,480,610 (GRCm39)	missense	probably damaging	0.99
R7939:Or10a3	UTSW	7	108,480,481 (GRCm39)	missense	probably benign	0.05
R8057:Or10a3	UTSW	7	108,480,571 (GRCm39)	missense	probably damaging	1.00
R8096:Or10a3	UTSW	7	108,480,248 (GRCm39)	nonsense	probably null
R8472:Or10a3	UTSW	7	108,479,973 (GRCm39)	missense	possibly damaging	0.95
R8766:Or10a3	UTSW	7	108,480,453 (GRCm39)	missense	probably benign	0.05
R9283:Or10a3	UTSW	7	108,480,289 (GRCm39)	missense	probably benign	0.03
R9570:Or10a3	UTSW	7	108,480,504 (GRCm39)	missense	possibly damaging	0.95
R9763:Or10a3	UTSW	7	108,480,874 (GRCm39)	start gained	probably benign
X0066:Or10a3	UTSW	7	108,480,679 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCACAGTAGCCACCATGATC -3'
(R):5'- ACTCCAAGGGCAGATGTTTGG -3'

Sequencing Primer
(F):5'- CATGATCCCAGATACCCATGAG -3'
(R):5'- CCTGGTAATTTATCTGGTGACCCTG -3'

Posted On

2019-06-26